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Principios para la elaboración del Atlas de incidencia delictiva de las principales ciudades de Quintana Roo

Thomas Ihl, Oscar Frausto, Javier Tun Chim, Celina Izquierdo, Manfred Rolfes
2009 Teoría y Praxis  
Quintana Roo, atlas, incidencia delictiva, mapeo del delito. tlas, incidencia delictiva, mapeo del delito.  ...  El presente artículo trata los principios teóricos y metodólogicos para la elaboración del atlas del delito de Quintana Roo, el cual se basa en la experiencia acumulada por el Observatorio Urbano de la  ...  Roo a través de la Generación, Manejo y Uso del Atlas de incidencia delictiva (Clave: Qroo-2006-C01-55797); a la Universidad de Quintana Roo, campus Cozumel; a la Universidad del Caribe; al Observatorio  ... 
doi:10.22403/uqroomx/typ06/05 fatcat:wspdqeutbrg4tlyinw464zn4r4

Methodological limitations to a study on interpersonal therapy

J Louw Roos, Christa Kruger, Manfred Bohmer
2013 South African Journal of Psychiatry  
J L Roos C Krüger M W Böhmer Department of Psychiatry, University of Pretoria, Gauteng, South Africa 1. Moosa MYH, Jeenah FY.  ... 
doi:10.7196/sajp.457 fatcat:c3hwvpy7mjeqlmw7kryf6lidpu

Methodological limitations to a study on interpersonal therapy

J Louw Roos, Christa Kruger, Manfred Bohmer
2013 South African Journal of Psychiatry  
J L Roos C Krüger M W Böhmer Department of Psychiatry, University of Pretoria, Gauteng, South Africa 1. Moosa MYH, Jeenah FY.  ... 
doi:10.4102/sajpsychiatry.v19i2.457 fatcat:ltk4w2n4hzh3pj76p3qzol74su

Vitamin D: a critical and essential micronutrient for human health

Igor Bendik, Angelika Friedel, Franz F. Roos, Peter Weber, Manfred Eggersdorfer
2014 Frontiers in Physiology  
Vitamin D is a micronutrient that is needed for optimal health throughout the whole life. Vitamin D 3 (cholecalciferol) can be either synthesized in the human skin upon exposure to the UV light of the sun, or it is obtained from the diet. If the photoconversion in the skin due to reduced sun exposure (e.g., in wintertime) is insufficient, intake of adequate vitamin D from the diet is essential to health. Severe vitamin D deficiency can lead to a multitude of avoidable illnesses; among them are
more » ... ell-known bone diseases like osteoporosis, a number of autoimmune diseases, many different cancers, and some cardiovascular diseases like hypertension are being discussed. Vitamin D is found naturally in only very few foods. Foods containing vitamin D include some fatty fish, fish liver oils, and eggs from hens that have been fed vitamin D and some fortified foods in countries with respective regulations. Based on geographic location or food availability adequate vitamin D intake might not be sufficient on a global scale. The International Osteoporosis Foundation (IOF) has collected the 25-hydroxy-vitamin D plasma levels in populations of different countries using published data and developed a global vitamin D map. This map illustrates the parts of the world, where vitamin D did not reach adequate 25-hydroxyvitamin D plasma levels: 6.7% of the papers report 25-hydroxyvitamin D plasma levels below 25 nmol/L, which indicates vitamin D deficiency, 37.3% are below 50 nmol/Land only 11.9% found 25-hydroxyvitamin D plasma levels above 75 nmol/L target as suggested by vitamin D experts. The vitamin D map is adding further evidence to the vitamin D insufficiency pandemic debate, which is also an issue in the developed world. Besides malnutrition, a condition where the diet does not match to provide the adequate levels of nutrients including micronutrients for growth and maintenance, we obviously have a situation where enough nutrients were consumed, but lacked to reach sufficient vitamin D micronutrient levels. The latter situation is known as hidden hunger. The inadequate vitamin D status impacts on health care costs, which in turn could result in significant savings, if corrected. Since little is known about the effects on the molecular level that accompany the pandemic like epigenetic imprinting, the insufficiency-triggered gene regulations or the genetic background influence on the body to maintain metabolic resilience, future research will be needed. The nutrition community is highly interested in the molecular mechanism that underlies the vitamin D insufficiency caused effect. In recent years, novel large scale technologies have become available that allow the simultaneous acquisition of transcriptome, epigenome, proteome, or metabolome data in cells of organs. These important methods are now used for nutritional approaches summarized in emerging scientific fields of nutrigenomics, nutrigenetics, or nutriepigenetics. It is believed that with the help of these novel concepts further understanding can be generated to develop future sustainable nutrition solutions to safeguard nutrition security.
doi:10.3389/fphys.2014.00248 pmid:25071593 pmcid:PMC4092358 fatcat:bwdry6nrofbaxakttrympgjkx4

Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U

Qun Ren, Guy de Roo, Bernard Witholt, Manfred Zinn, Linda Thöny-Meyer
2010 BMC Microbiology  
doi:10.1186/1471-2180-10-254 pmid:20937103 pmcid:PMC2959000 fatcat:mq66ibh4craklm7fjqdmqyedju

Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1

Qun Ren, Guy de Roo, Bernard Witholt, Manfred Zinn, Linda Thöny-Meyer
2009 Microbial Cell Factories  
Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two different class II polymerases, encoded by phaC1 and phaC2. Although numerous studies have been carried out on PHA
more » ... merases and they are well characterized at the molecular level, the biochemical properties of the class II polymerases have not been studied in detail. Previously we and other groups purified the polymerases, however, the activities of the purified enzymes were several magnitude lower than the granule-bound enzymes. It is problematic to study the intrinsic properties of these enzymes with such low activities, although they are pure. Results: PHA polymerase 1 (PhaC1) and PHA polymerase 2 (PhaC2) from P. putida GPo1 were overexpressed in the PHA-negative host P. putida GPp104 and purified from isolated PHA granules. Only minor activity (two to three orders of magnitude lower than that of the granule bound proteins) could be recovered when the enzymes were purified to homogeneity. Therefore, kinetic properties and substrate ranges were determined for the granule bound polymerases. The polymerases differed significantly with respect to their association with PHA granules, enzyme kinetics and substrate specificity. PhaC2 appeared to bind PHA granules more tightly than PhaC1. When R-3-hydroxyoctanoic acid was used as substrate, the granule-bound PhaC1 exhibited a Km of 125 (± 35) μM and a Vmax of 40.8 (± 6.2) U/mg PhaC1, while a Km of 37 (± 10) μM and a Vmax of 2.7 (± 0.7) U/mg PhaC2 could be derived for the granule-bound PhaC2. Granulebound PhaC1 showed a strong preference for medium chain length (mcl-) 3-hydroxyacly-CoAs, with highest affinity towards 3-hydroxydecanoyl-CoA (40 U/mg PhaC1). Granule-bound PhaC2 demonstrated a far broader specificity ranging from short chain length up to long chain length substrates. Activity increased with increasing chain length with a maximum activity for 3-hydroxyacyl-CoAs containing 12 or more C-atoms. Conclusion: The kinetic properties and substrate ranges were determined for both granule bound polymerases. Evidence was provided for the first time that two PHA polymerases exhibited significant differences in granule release and in vitro activity profiles, suggesting that there are substantial functional differences between granule bound PhaC1 and PhaC2.
doi:10.1186/1475-2859-8-60 pmid:19925642 pmcid:PMC2788523 fatcat:becjk7gj7jejpbsi3kasfb63oi

Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida

Qun Ren, Guy de Roo, Jan B. van Beilen, Manfred Zinn, Birgit Kessler, Bernard Witholt
2005 Applied Microbiology and Biotechnology  
hydroxyoctanoyl-CoA was purified by using a preparative C8-spherisorb-RP column (Bischoff, Germany) together with a preparative HPLC system (Labomatic liquid chromatography system, Allschwil, Switzerland) (de Roo  ... 
doi:10.1007/s00253-005-1995-1 pmid:15846484 fatcat:fpydeg5ndfa7ziqwh6vyqz76ce

Students' performing of practical research tasks for their scientific qualification - an approach within the family practice internship in undergraduate education

Dirk Moßhammer, Marco J Roos, Andrea Kronenthaler, Gernot Lorenz, Manfred Eissler, Stefanie Joos
2011 GMS Zeitschrift für Medizinische Ausbildung  
Roos 2,3 both practical medical and basic research competencies.  ...  Therefore the aim of this article is to develop a didactic Manfred Eissler 1 and methodological concept for research-based teaching and learning based on the initial results from the block placement  ... 
doi:10.3205/zma000736 pmid:21818234 pmcid:PMC3149465 fatcat:aukhhdifkffezfhbaaabmz3xdm

The Conduit System Transports Soluble Antigens from the Afferent Lymph to Resident Dendritic Cells in the T Cell Area of the Lymph Node

Michael Sixt, Nobuo Kanazawa, Manuel Selg, Thomas Samson, Gunnel Roos, Dieter P. Reinhardt, Reinhard Pabst, Manfred B. Lutz, Lydia Sorokin
2005 Immunity  
can phagocytose antigens and, subsequently, traffic via 22185 Lund the afferent lymphatics into the T cell area of the draining Sweden lymph node to initiate immune responses (Cavanagh 2 Department of Dermatology and Von Andrian, 2002; Manickasingham and Reis e University of Erlangen Sousa, 2001; Randolph, 2001). This pathway is well char-91052 Erlangen acterized, and there is recent evidence that steady state Germany migration of DC into the lymph node also occurs in 3 Department of
more » ... l Medicine I the healthy organism, which may serve to continuously University of Erlangen tolerize T cells against self antigens (Lutz and Schuler, 91054 Erlangen 2002; Steinman et al., 2003). A second pathway of anti-Germany gen delivery is less well defined and functions indepen-4 Department of Anatomy and Cell Biology dently of cellular trafficking along the lymphatics. Sev-McGill University eral studies have shown that peripherally applied Montreal H3A 2B2 soluble antigen is taken up, presented, and cross pre-Canada sented by resident DC in the T cell area of the draining 5 Department of Functional and Applied Anatomy lymph node. This happens before there is any detectable Medical School of Hannover immigration of DC from the periphery (Ingulli et al., 2002; 30625 Hannover Itano et al., 2003; Maurer et al., 2002; Pior et al., 1999). Germany These pathways of antigen delivery result in two temporally distinct "waves" of antigen presentation within the lymph node, which have been shown to induce function-Summary ally different T cell responses (Itano and Jenkins, 2003). However, to date, the physical means of transport of Resident dendritic cells (DC) within the T cell area of the soluble antigen from the injection site to the DC has the lymph node take up soluble antigens that enter not been identified, although it was speculated as early via the afferent lymphatics before antigen carrying DC as 1964 that the reticular fiber network of the lymph arrive from the periphery. The reticular network within node may play a central role in this process (Moe, 1964). the lymph node is a conduit system forming the infra- The reticular network is a three-dimensional frame of structure for the fast delivery of soluble substances collagen fibers that is closely ensheathed by the nonhefrom the afferent lymph to the lumen of high endothematopoietic, fibroblastic reticular cells (FRC), resulting lial venules (HEVs). Using high-resolution light microsin a scaffold that physically connects the subcapsular copy and 3D reconstruction, we show here that these and paracortical sinuses with the walls of the blood conduits are unique basement membrane-like struc- vessels (Anderson and Anderson, 1975; Anderson and tures ensheathed by fibroblastic reticular cells with Shaw, 1993; Gretz et al., 1997; Sainte-Marie and Peng, occasional resident DC embedded within this cell 1986; Ushiki et al., 1995). The FRC are tightly interconlayer. Conduit-associated DC are capable of taking up nected, resulting in a compartment that is separate from and processing soluble antigens transported within the T and B cell microenvironments. The concept that the conduits, whereas immigrated mature DC occur this network can serve as a conduit system for the transremote from the reticular fibers. The conduit system port of fluid and soluble substances within the lymph is, therefore, not a closed compartment that shuttles node stems from early morphological studies (Anderson substances through the lymph node but represents and Anderson, 1975; Sainte-Marie and Peng, 1986). In the morphological equivalent to the filtering function addition, recent studies by Shaw and coworkers (Gretz of the lymph node. et al., 2000) have shown that the conduit system allows low molecular weight substances (below 70 kDa), car-Introduction ried within the afferent lymph, to move directly from the subcapsular sinus to the lumen of HEVs without Most primary adaptive immune responses are initiated percolating through the lymphocyte compartment. in the T cell area of the lymph node. At this site, naive The physiological significance of this conduit system T cells, recirculating from the blood via HEVs, encounter was recently shown in two studies (Baekkevold et al., DC that present processed peptides of previously ac-2001; Palframan et al., 2001): the authors demonstrated quired antigens in an MHC context (Banchereau and that within minutes after subcutaneous injection, che-Steinman, 1998). Despite enormous progress in the field mokines are presented on the lumen of HEVs where of DC biology and rapidly accumulating data on molecuthey can act to recruit leukocytes. The fast delivery of chemokines occurred via the conduit system and was interpreted as a "remote control" function that serves *Correspondence: Immunity 20
doi:10.1016/j.immuni.2004.11.013 pmid:15664156 fatcat:f5ctknb3rvh6dbnbvehliplu54

A Novel System of Polymorphic and Diverse NK Cell Receptors in Primates

Anne Averdam, Beatrix Petersen, Cornelia Rosner, Jennifer Neff, Christian Roos, Manfred Eberle, Fabienne Aujard, Claudia Münch, Werner Schempp, Mary Carrington, Takashi Shiina, Hidetoshi Inoko (+8 others)
2009 PLoS Genetics  
There are two main classes of natural killer (NK) cell receptors in mammals, the killer cell immunoglobulin-like receptors (KIR) and the structurally unrelated killer cell lectin-like receptors (KLR). While KIR represent the most diverse group of NK receptors in all primates studied to date, including humans, apes, and Old and New World monkeys, KLR represent the functional equivalent in rodents. Here, we report a first digression from this rule in lemurs, where the KLR (CD94/NKG2) rather than
more » ... IR constitute the most diverse group of NK cell receptors. We demonstrate that natural selection contributed to such diversification in lemurs and particularly targeted KLR residues interacting with the peptide presented by MHC class I ligands. We further show that lemurs lack a strict ortholog or functional equivalent of MHC-E, the ligands of nonpolymorphic KLR in "higher" primates. Our data support the existence of a hitherto unknown system of polymorphic and diverse NK cell receptors in primates and of combinatorial diversity as a novel mechanism to increase NK cell receptor repertoire.
doi:10.1371/journal.pgen.1000688 pmid:19834558 pmcid:PMC2757895 fatcat:3bnmbvh3pvedngrwvfrpnptinu

SAMHD1 mutations in mantle cell lymphoma: identification as disease driver conferring in vitro resistance to nucleoside analogues [article]

Marco M Bühler, Junyan Lu, Sebastian Scheinost, Ferran Nadeu, Damien Roos-Weil, Manfred Hensel, Tharshika Thavayogarajah, Holger Moch, Markus G Manz, Eugenia Haralambieva, Ewerton Marques Maggio, Silvia Bea (+5 others)
2020 bioRxiv   pre-print
The genomic landscape of mantle cell lymphoma (MCL) includes frequent alterations of TP53, ATM, CCND1 and KMT2D. Thus far, the mutational landscape provides little information for treatment stratification. We characterized a cohort of MCL by targeted next generation sequencing and discovered SAMHD1 as a novel recurrently mutated gene (8.5% of investigated cases, 4/47 samples). Furthermore, we provide evidence of in vitro resistance of SAMHD1 mutated patient-derived MCL cells to cytarabine and fludarabine.
doi:10.1101/2020.06.09.140376 fatcat:whrnpuuibnfvnpdnlrlrwhiuly

Homology-based inference sets the bar high for protein function prediction

Tobias Hamp, Rebecca Kassner, Stefan Seemayer, Esmeralda Vicedo, Christian Schaefer, Dominik Achten, Florian Auer, Ariane Boehm, Tatjana Braun, Maximilian Hecht, Mark Heron, Peter Hönigschmid (+8 others)
2013 BMC Bioinformatics  
doi:10.1186/1471-2105-14-s3-s7 pmid:23514582 pmcid:PMC3584931 fatcat:gwjjghihvrffdluv6lvlyzqoqu

Active site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone demonstrates the full activity of the refolded and purified tissue-type plasminogen activator variant BM 06.022

Ulrich Kohnert, Manfred Wozny, Manuel Llinas, Anita Roos, Stephan Fischer
1995 Applied Biochemistry and Biotechnology  
BM 06.022 is a tissue-type plasminogen activator deletion variant that is comprised of the kringle 2 and the protease domain of the native molecule. BM 06.022 is expressed as inactive inclusion bodies in E. coli and transferred into the active enzyme by an in vitro folding process. Active site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone provides evidence that the purified BM 06.022 is fully active and that misfolded species are completely removed by affinity chromatography
more » ... n ETI-Sepharose. The comparison of the kinetics of the inhibition of BM 06.022 with that of CHO-t-PA indicates that the active centers of both enzymes are rather similar. The further evaluation of the site of interaction of BM 06.022 and DnsEGRck by mass spectroscopy and amino acid sequence analysis revealed that the inhibitor is bound selectively to His322, which is part of the catalytic triad of this serine protease.
doi:10.1007/bf02783556 pmid:7495332 fatcat:7gatfmrwk5hz7av3rx5zvyfq2a

PredictProtein—an open resource for online prediction of protein structural and functional features

Guy Yachdav, Edda Kloppmann, Laszlo Kajan, Maximilian Hecht, Tatyana Goldberg, Tobias Hamp, Peter Hönigschmid, Andrea Schafferhans, Manfred Roos, Michael Bernhofer, Lothar Richter, Haim Ashkenazy (+8 others)
2014 Nucleic Acids Research  
PredictProtein is a meta-service for sequence analysis that has been predicting structural and functional features of proteins since 1992. Queried with a protein sequence it returns: multiple sequence alignments, predicted aspects of structure (secondary structure, solvent accessibility, transmembrane helices (TMSEG) and strands, coiled-coil regions, disulfide bonds and disordered regions) and function. The service incorporates analysis methods for the identification of functional regions
more » ... rf), homology-based inference of Gene Ontology terms (metastudent), comprehensive subcellular localization prediction (LocTree3), protein-protein binding sites (ISIS2), protein-polynucleotide binding sites (SomeNA) and predictions of the effect of point mu-tations (non-synonymous SNPs) on protein function (SNAP2). Our goal has always been to develop a system optimized to meet the demands of experimentalists not highly experienced in bioinformatics. To this end, the PredictProtein results are presented as both text and a series of intuitive, interactive and visually appealing figures. The web server and sources are available at
doi:10.1093/nar/gku366 pmid:24799431 pmcid:PMC4086098 fatcat:c5pmgwyx7zg4jjncj4eywixmf4

Circulating Haptoglobin and Metabolic Syndrome in Renal Transplant Recipients

Isidor Minović, Michele F. Eisenga, Ineke J. Riphagen, Else van den Berg, Jenny Kootstra-Ros, Anne-Roos S. Frenay, Harry van Goor, Gerald Rimbach, Tuba Esatbeyoglu, Andy P. Levy, Carlo A. J. M. Gaillard, Johanna M. Geleijnse (+4 others)
2017 Scientific Reports  
Haptoglobin (Hp) is an acute phase protein that has recently been linked to components of the metabolic syndrome (MetS). We aimed to evaluate Hp as marker of MetS, and to assess its association with long-term outcome in renal transplant recipients (RTR). We measured plasma Hp in a prospective cohort of 699 stable RTR and 149 healthy controls. Median plasma Hp concentration in RTR was 1.4 [interquartile range (IQR), 1.0-1.8] g/L, which was higher compared to 1.1 [0.9-1.4] g/L in controls (P <
more » ... 01). Hp was independently associated with the MetS (β = 0.10) (P = 0.005). During follow-up of 5.4 [4.8-6.1] years, 150 (21%) recipients died, of whom 60 (9%) due to cardiovascular causes, and 83 (12%) RTR developed graft failure. High (≥2.0 g/L) and low (≤0.9 g/L) plasma Hp were associated with increased risk of mortality (HR's 2.3 [1.3-4.1] and 1.9 [1.0-3.5], resp.), predominantly cardiovascular. The association of high Hp lost significance upon adjustment for inflammation markers (HR 1.5 [0.8-2.7]), while low Hp was independently associated with mortality (HR 2.2 [1.2-4.0]). Hp was not associated with graft failure (P = 0.49). In conclusion, plasma Hp is independently associated with MetS in RTR. Importantly, high and low Hp are associated with increased mortality risk, independent of MetS. Haptoglobin (Hp) is a hepatic glycoprotein that is traditionally used to monitor in vivo hemolysis 1 . As such, Hp is assessed in transplant recipients as marker of calcineurin-induced microangiopathy short after transplantation. However, the role of Hp in transplant recipients under non-hemolytic circumstances, e.g. late after transplantation, has not been fully evaluated. Previous studies describing the non-hemolytic significance of Hp have primarily been cross-sectional in nature and have mainly focused on the role of Hp in inflammation 2-4 . These studies have established Hp as a positive acute phase protein as a result of induced gene transcription by pro-inflammatory cytokines. Besides inflammation, Hp has recently gained additional attention as it has been related to key components of the metabolic syndrome (MetS) 5-11 . The MetS is increasingly prevalent among renal transplant recipients (RTR) where it has been found to be an independent risk factor for development of post-transplantation diabetes mellitus, cardiovascular disease, mortality, and graft failure 12,13 . Since Hp has been related to key components of the MetS, Hp could possibly be interpreted as a marker of the MetS. However, as the MetS per se is associated with inflammation 14,15 , it is difficult to distinguish to what extent the previously found associations of Hp with components of the MetS are attributable to inflammation. Furthermore, it is not known whether Hp is associated with long-term outcome in RTR and it is also not known whether potential prospective associations of Hp are influenced by the MetS or inflammation.
doi:10.1038/s41598-017-14302-2 pmid:29079835 pmcid:PMC5660219 fatcat:3i2wmh2vx5bsheseewvlolt5tm
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