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Circulating extracellular miR-22, miR-155, and miR-365 as candidate biomarkers to assess transport-related stress in turkeys
2016
Animal
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The expression levels of five circulating miRNA, namely miR-22, miR-155-5p, miR-181a-3p, miR-204 and miR-365-3p, were detected and assessed by quantitative polymerase chain reaction using TaqMan®probes ...
A panel of three stress-responsive miRNA, miR-22, miR-155 and miR-365 were identified; their expression levels were significantly higher after road transportation and the area under the curve (AUC) were ...
Statistical analysis was performed using XLSTAT for Windows (Addinsoft, New York, NY, USA) and MedCalc 14.0 (MedCalc Software bvba, Ostend, Belgium). ...
doi:10.1017/s1751731115003043
pmid:26760121
fatcat:74tlbxemend75oyiwntd2b6kuu
miR-372 and miR-373 enhance the stemness of colorectal cancer cells by repressing differentiation signaling pathways
2018
Molecular Oncology
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Numerous new targets of miR-372/373 were identified, including SPOP, VDR and SETD7, all of which are factors important for cell differentiation. ...
These results provide new insights for understanding the function and mechanisms of stem cell-specific microRNAs in the development of metastasis and drug resistance in CRC. ...
Sphere formation assay Cells (3 9 10 3 ) were seeded in six-well ultra-low attachment surface plates (Corning, New York, NY, USA) and cultured in DMEM/F12 serum-free medium (Gibco, Life Technologies, Scoresby ...
doi:10.1002/1878-0261.12376
pmid:30171794
pmcid:PMC6210048
fatcat:x3b3brdkajbqzgwp5jaxkkyg4u
MiR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1
2011
Oncogene
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-carrying mutated seed sequences of miR-200b or miR-15b at its 3 0 -UTR recapitulated chemotherapy-induced EMT. ...
Ectopic expression of miR-200b and miR-15b with miRNA mimics effectively reversed the phenotype of EMT in CAL27-res and SCC25-res cells, and sensitized them to chemotherapy, but inhibition of miR-200b ...
Furthermore, we examined the invasion and migration of chemo-resistant cells using Boyden chamber (Corning, New York, NY, USA) assays. ...
doi:10.1038/onc.2011.263
pmid:21725369
fatcat:disx7u2o5re2je5y4ldr6qvq5q
MiR‐195 and miR‐497 suppress tumorigenesis in lung cancer by inhibiting SMURF2‐induced TGF‐β receptor Ⅰ ubiquitination
2019
Molecular Oncology
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These findings demonstrate that miR-195 and miR-497 act as a tumor suppressor by suppressing ubiquitination-mediated degradation of TGF-β receptors through SMURF2, and suggest that miR-195 and miR-497 ...
Both miR-195 and miR-497 bind to the 3'-UTR of the SMURF2 mRNA and inhibit SMURF2 expression. ...
Invasion assay Cell invasion assays were performed in 6.5-mm Transwells (8.0 lm pore size) (Coring Incorporation, New York, NY, USA). ...
doi:10.1002/1878-0261.12581
pmid:31581360
pmcid:PMC6887584
fatcat:lm2t6fvcjnex7abormvxlgxdma
Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation
2014
Molecular Medicine Reports
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The association between miR-9 and ALCAM mRNA expression was determined by quantitative polymerase chain reaction. ...
The expression of miR-9 was reduced and inversely correlated with ALCAM mRNA levels in GC tissues and cell lines. ...
For the detection of sALCAM, Costar flexible 96-well microtiter plates (Corning Incorporated, New York, NY, USA) were coated with a monoclonal human anti-mouse ALCAM antibody (#MAB6561; R&D Systems, Inc ...
doi:10.3892/mmr.2014.2933
pmid:25395097
fatcat:uvrf74ua45hntawiy5vh6wqu7a
miR‑127 aggravates myocardial failure by promoting the TGF‑β1/Smad3 signaling
2018
Molecular Medicine Reports
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the functions of miR-127 in the development of myocardial failure. ...
The results confirmed that miR-127 regulates the expression of TGF-β1/Smad3. ...
The animal model was established as previously described using 6 intraperitoneal injections with 2.5 mg/kg DOX (Adriamycin ® ; Pfizer, Inc., New York, NY, USA) every 48 h to achieve an accumulative dose ...
doi:10.3892/mmr.2018.9514
fatcat:7o7fqsxoufcspgb2mpc345uhvu
ssc-miR-185 targets CDC42 and promotes the proliferation of intestinal porcine epithelial cell
2020
Asian-Australasian Journal of Animal Sciences
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We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2, and explored the effect of miR-185 on the proliferation of IPEC-J2 cells. ...
Further we found that the mRNA and protein expression level of CDC42 were down-regulated after transfection with miR-185 mimics, while the opposite trend was observed after transfection with miR-185 inhibitor ...
The cells were cultured in DMEM/F12 medium (HyClone, New York, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Inc., New York, USA), and 1% penicillinstreptomycin at ...
doi:10.5713/ajas.20.0325
pmid:33152231
pmcid:PMC8100468
fatcat:opilndevdnhotpvgrkmielf3qe
Increased miR-132-3p expression is associated with chronic neuropathic pain
2016
Experimental Neurology
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Taken together these results indicate a pro-nociceptive effect of miR-132-3p in chronic neuropathic pain. ...
For this purpose miR-132-3p expression levels were measured in dorsal root ganglia and spinal cord of rats. ...
All rats received subcutaneous lactated Ringer's solution (1 cc/50 g body weight, Baxter HealthCare Corporation, Deerfield, IL, USA) with carprofen (5 mg/kg, Rimadyl Pfizer Inc., New York, NY, USA) immediately ...
doi:10.1016/j.expneurol.2016.06.025
pmid:27349406
pmcid:PMC4992589
fatcat:ve32o3ohkzdb7jqf5vmkcrqw4m
Decreased miR-134 expression and its tumor-suppressive function in human osteosarcoma
2015
Genetics and Molecular Research
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Dysregulation of microRNA (miR) is often associated with cancer development and progression. Aberrant expression of miR-134 has been found in some types of cancer. ...
MiR-134 was significantly downregulated in osteosarcoma cell lines and clinical specimens. ...
Transwell invasion and migration assays Cell migration and invasion assays were performed using a 24-well transwell plate (8-mm pore size; Corning, New York, NY, USA). ...
doi:10.4238/2015.december.14.4
pmid:26681023
fatcat:autfxlzfcvccxdyajgbkqd5qfa
Towards automatic analysis of social interaction patterns in a nursing home environment from video
2004
Proceedings of the 6th ACM SIGMM international workshop on Multimedia information retrieval - MIR '04
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This research is supported by the National Science Foundation (USA) through project CareMedia No. 0205219. ...
Concisely, we can omit the subjects (persons) and interpret the video (2-4) as: (2) standing conversation -> wheelchair pushing;
, New York, NY, USA. ...
MIR'04, October 15-16, 2004 Interaction is mutual or reciprocal action that involves two or more people. Social interaction produces many visual patterns. ...
doi:10.1145/1026711.1026757
dblp:conf/mir/ChenYW04
fatcat:b65pmbngsnarlitmvmorclcbm4
Decreased expression of miR-874 and its tumor suppressive function in human colorectal cancer
2016
Genetics and Molecular Research
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Taken together, these findings indicate that miR-874 may act as a tumor suppressor in CRC, and may serve as a novel therapeutic target for miR-based therapy. ...
For example, aberrant expression of miR-874 has been found in some types of cancer. However, miR-874 expression and its clinical significance in colorectal cancer (CRC) have not yet been explored. ...
Cell invasion and migration assays Cell invasion and migration assays were performed using 24-well transwell plates (8-mm pore size, Corning, New York, NY, USA). ...
doi:10.4238/gmr.15028077
pmid:27173188
fatcat:xcuku4ju4zbspg4o7ab445unku
Methylation-associated miR-193b silencing activates master drivers of aggressive prostate cancer
2019
Molecular Oncology
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Knockdown of miR-193b targets FOXM1 and RRM2 in prostate cancer cells phenocopied overexpression of miR-193b. ...
We found that a majority of the top 150 genes downregulated when miR-193b was overexpressed in liposarcoma are overexpressed in metastatic prostate cancer and that 41 miR-193b target genes overlapped with ...
., Bavarian Nordic Immunotherapeutics, DRGT, GE Healthcare, Janssen, Metamark, New England Research Institutes, Inc., OncoCellMDX, Progenity, Sanofi, Seer Biosciences, Tarveda Therapeutics, and Thermo ...
doi:10.1002/1878-0261.12536
pmid:31225930
pmcid:PMC6717747
fatcat:owgu7nej6fgmbnokmy7bi4zslu
The American Presidency and the 25th Amendment in Contemporary TV Series: Fiction, Reality, and the Warped Mir
2012
TV Series
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Not only has actor Dennis Haysbert, 15 Read in particular Brian Stelter's article in The New York Times online, October 30, 2008: "Following the Script: Obama, McCain, and 'The West Wing'" (last consulted ...
See this recent 2011 Pew Poll: http://peoplepress.org/2011/06/02/section-2-candidate-traits-and-experience/
See: "Feminist Fantasy: Geena Davis in the Oval Office", Alessandra Stanley, The New York ...
doi:10.4000/tvseries.1095
fatcat:hhmdr2uvvjhlpcrq3w6m3ljm2u
Abstracts from the Astrobiology Science Conference 2004
2004
International Journal of Astrobiology
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Phylogenetic analysis of chromosomal data Ward Wheeler, Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th St., New York, NY 10024-5192; wheeler@amnh.org Large-scale ...
Astrobiology In Museums Martin Weiss, PhD, New York Hall of Science mweiss@nyscience.org;
Acknowledgements We wish to thank The University of Hawaii and NASA-NAI (USA), PPARC (UK), and Osaka Vacuum ( ...
Mars Public and media reactions to 2003/2004 Mars missions: A case for the Internet as the primary source of astrobiology-related news Carol A. ...
doi:10.1017/s1473550404001648
fatcat:7edgphlk6bb2xf73w3rrb2vc3m
MiR-9 is involved in TGF-β1-induced lung cancer cell invasion and adhesion by targeting SOX7
2017
Journal of Cellular and Molecular Medicine
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Moreover, SOX7 expression was negatively correlated with miR-9 expression. miR-9 knockdown or SOX7 overexpression could suppress TGF-b1-induced NSCLC cell invasion and adhesion. miR-9 directly targets ...
MicroRNA (miR)-9 plays different roles in different cancer types. ...
Cell invasion assay The transwell inserts with 8-lm pore polycarbonate membrane (Corning, New York, NY, USA) were pre-coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). 1 9 10 5 cells in serum-free ...
doi:10.1111/jcmm.13120
pmid:28266181
pmcid:PMC5571535
fatcat:xxuczb5pqfbbfnuj6v44ypbxwq
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