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Circulating extracellular miR-22, miR-155, and miR-365 as candidate biomarkers to assess transport-related stress in turkeys

C. Lecchi, A. T. Marques, M. Redegalli, S. Meani, L. J. Vinco, V. Bronzo, F. Ceciliani
2016 Animal  
The expression levels of five circulating miRNA, namely miR-22, miR-155-5p, miR-181a-3p, miR-204 and miR-365-3p, were detected and assessed by quantitative polymerase chain reaction using TaqMan®probes  ...  A panel of three stress-responsive miRNA, miR-22, miR-155 and miR-365 were identified; their expression levels were significantly higher after road transportation and the area under the curve (AUC) were  ...  Statistical analysis was performed using XLSTAT for Windows (Addinsoft, New York, NY, USA) and MedCalc 14.0 (MedCalc Software bvba, Ostend, Belgium).  ... 
doi:10.1017/s1751731115003043 pmid:26760121 fatcat:74tlbxemend75oyiwntd2b6kuu

miR-372 and miR-373 enhance the stemness of colorectal cancer cells by repressing differentiation signaling pathways

Lu-Qin Wang, Peng Yu, Bin Li, Yan-Hua Guo, Zi-Rui Liang, Ling-Ling Zheng, Jian-Hua Yang, Hui Xu, Shun Liu, Li-Si Zheng, Hui Zhou, Liang-Hu Qu
2018 Molecular Oncology  
Numerous new targets of miR-372/373 were identified, including SPOP, VDR and SETD7, all of which are factors important for cell differentiation.  ...  These results provide new insights for understanding the function and mechanisms of stem cell-specific microRNAs in the development of metastasis and drug resistance in CRC.  ...  Sphere formation assay Cells (3 9 10 3 ) were seeded in six-well ultra-low attachment surface plates (Corning, New York, NY, USA) and cultured in DMEM/F12 serum-free medium (Gibco, Life Technologies, Scoresby  ... 
doi:10.1002/1878-0261.12376 pmid:30171794 pmcid:PMC6210048 fatcat:x3b3brdkajbqzgwp5jaxkkyg4u

MiR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1

L Sun, Y Yao, B Liu, Z Lin, L Lin, M Yang, W Zhang, W Chen, C Pan, Q Liu, E Song, J Li
2011 Oncogene  
-carrying mutated seed sequences of miR-200b or miR-15b at its 3 0 -UTR recapitulated chemotherapy-induced EMT.  ...  Ectopic expression of miR-200b and miR-15b with miRNA mimics effectively reversed the phenotype of EMT in CAL27-res and SCC25-res cells, and sensitized them to chemotherapy, but inhibition of miR-200b  ...  Furthermore, we examined the invasion and migration of chemo-resistant cells using Boyden chamber (Corning, New York, NY, USA) assays.  ... 
doi:10.1038/onc.2011.263 pmid:21725369 fatcat:disx7u2o5re2je5y4ldr6qvq5q

MiR‐195 and miR‐497 suppress tumorigenesis in lung cancer by inhibiting SMURF2‐induced TGF‐β receptor Ⅰ ubiquitination

Dong‐Kyu Chae, Jinyoung Park, Moonsoo Cho, Eunmi Ban, Mihue Jang, Young Sook Yoo, Eunice EunKyeong Kim, Ja‐Hyun Baik, Eun Joo Song
2019 Molecular Oncology  
These findings demonstrate that miR-195 and miR-497 act as a tumor suppressor by suppressing ubiquitination-mediated degradation of TGF-β receptors through SMURF2, and suggest that miR-195 and miR-497  ...  Both miR-195 and miR-497 bind to the 3'-UTR of the SMURF2 mRNA and inhibit SMURF2 expression.  ...  Invasion assay Cell invasion assays were performed in 6.5-mm Transwells (8.0 lm pore size) (Coring Incorporation, New York, NY, USA).  ... 
doi:10.1002/1878-0261.12581 pmid:31581360 pmcid:PMC6887584 fatcat:lm2t6fvcjnex7abormvxlgxdma

Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation

MIN YE, YAN-LEI DU, YU-QIANG NIE, ZHI-WEI ZHOU, JIE CAO, YING-FEI LI
2014 Molecular Medicine Reports  
The association between miR-9 and ALCAM mRNA expression was determined by quantitative polymerase chain reaction.  ...  The expression of miR-9 was reduced and inversely correlated with ALCAM mRNA levels in GC tissues and cell lines.  ...  For the detection of sALCAM, Costar flexible 96-well microtiter plates (Corning Incorporated, New York, NY, USA) were coated with a monoclonal human anti-mouse ALCAM antibody (#MAB6561; R&D Systems, Inc  ... 
doi:10.3892/mmr.2014.2933 pmid:25395097 fatcat:uvrf74ua45hntawiy5vh6wqu7a

miR‑127 aggravates myocardial failure by promoting the TGF‑β1/Smad3 signaling

Hainian Xu, Fengmei Li
2018 Molecular Medicine Reports  
the functions of miR-127 in the development of myocardial failure.  ...  The results confirmed that miR-127 regulates the expression of TGF-β1/Smad3.  ...  The animal model was established as previously described using 6 intraperitoneal injections with 2.5 mg/kg DOX (Adriamycin ® ; Pfizer, Inc., New York, NY, USA) every 48 h to achieve an accumulative dose  ... 
doi:10.3892/mmr.2018.9514 fatcat:7o7fqsxoufcspgb2mpc345uhvu

ssc-miR-185 targets CDC42 and promotes the proliferation of intestinal porcine epithelial cell

Wei Wang, Pengfei Wang, Kaihui Xie, Ruirui Luo, Xiaoli Gao, Zunqiang Yan, Xiaoyu Huang, Qiaoli Yang, Shuangbao Gun
2020 Asian-Australasian Journal of Animal Sciences  
We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2, and explored the effect of miR-185 on the proliferation of IPEC-J2 cells.  ...  Further we found that the mRNA and protein expression level of CDC42 were down-regulated after transfection with miR-185 mimics, while the opposite trend was observed after transfection with miR-185 inhibitor  ...  The cells were cultured in DMEM/F12 medium (HyClone, New York, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Inc., New York, USA), and 1% penicillinstreptomycin at  ... 
doi:10.5713/ajas.20.0325 pmid:33152231 pmcid:PMC8100468 fatcat:opilndevdnhotpvgrkmielf3qe

Increased miR-132-3p expression is associated with chronic neuropathic pain

M. Leinders, N. Üçeyler, R.A. Pritchard, C. Sommer, L.S. Sorkin
2016 Experimental Neurology  
Taken together these results indicate a pro-nociceptive effect of miR-132-3p in chronic neuropathic pain.  ...  For this purpose miR-132-3p expression levels were measured in dorsal root ganglia and spinal cord of rats.  ...  All rats received subcutaneous lactated Ringer's solution (1 cc/50 g body weight, Baxter HealthCare Corporation, Deerfield, IL, USA) with carprofen (5 mg/kg, Rimadyl Pfizer Inc., New York, NY, USA) immediately  ... 
doi:10.1016/j.expneurol.2016.06.025 pmid:27349406 pmcid:PMC4992589 fatcat:ve32o3ohkzdb7jqf5vmkcrqw4m

Decreased miR-134 expression and its tumor-suppressive function in human osteosarcoma

Y. Bao, L. Peng, J. Ma, K. Liu, W. Li
2015 Genetics and Molecular Research  
Dysregulation of microRNA (miR) is often associated with cancer development and progression. Aberrant expression of miR-134 has been found in some types of cancer.  ...  MiR-134 was significantly downregulated in osteosarcoma cell lines and clinical specimens.  ...  Transwell invasion and migration assays Cell migration and invasion assays were performed using a 24-well transwell plate (8-mm pore size; Corning, New York, NY, USA).  ... 
doi:10.4238/2015.december.14.4 pmid:26681023 fatcat:autfxlzfcvccxdyajgbkqd5qfa

Towards automatic analysis of social interaction patterns in a nursing home environment from video

Datong Chen, Jie Yang, Howard D. Wactlar
2004 Proceedings of the 6th ACM SIGMM international workshop on Multimedia information retrieval - MIR '04  
This research is supported by the National Science Foundation (USA) through project CareMedia No. 0205219.  ...  Concisely, we can omit the subjects (persons) and interpret the video (2-4) as: (2) standing conversation -> wheelchair pushing; , New York, NY, USA.  ...  MIR'04, October 15-16, 2004 Interaction is mutual or reciprocal action that involves two or more people. Social interaction produces many visual patterns.  ... 
doi:10.1145/1026711.1026757 dblp:conf/mir/ChenYW04 fatcat:b65pmbngsnarlitmvmorclcbm4

Decreased expression of miR-874 and its tumor suppressive function in human colorectal cancer

X.J. Wang, M. Xia, W.P. Bi
2016 Genetics and Molecular Research  
Taken together, these findings indicate that miR-874 may act as a tumor suppressor in CRC, and may serve as a novel therapeutic target for miR-based therapy.  ...  For example, aberrant expression of miR-874 has been found in some types of cancer. However, miR-874 expression and its clinical significance in colorectal cancer (CRC) have not yet been explored.  ...  Cell invasion and migration assays Cell invasion and migration assays were performed using 24-well transwell plates (8-mm pore size, Corning, New York, NY, USA).  ... 
doi:10.4238/gmr.15028077 pmid:27173188 fatcat:xcuku4ju4zbspg4o7ab445unku

Methylation-associated miR-193b silencing activates master drivers of aggressive prostate cancer

Ying Z. Mazzu, Yuki Yoshikawa, Subhiksha Nandakumar, Goutam Chakraborty, Joshua Armenia, Lina E. Jehane, Gwo-Shu Mary Lee, Philip W. Kantoff
2019 Molecular Oncology  
Knockdown of miR-193b targets FOXM1 and RRM2 in prostate cancer cells phenocopied overexpression of miR-193b.  ...  We found that a majority of the top 150 genes downregulated when miR-193b was overexpressed in liposarcoma are overexpressed in metastatic prostate cancer and that 41 miR-193b target genes overlapped with  ...  ., Bavarian Nordic Immunotherapeutics, DRGT, GE Healthcare, Janssen, Metamark, New England Research Institutes, Inc., OncoCellMDX, Progenity, Sanofi, Seer Biosciences, Tarveda Therapeutics, and Thermo  ... 
doi:10.1002/1878-0261.12536 pmid:31225930 pmcid:PMC6717747 fatcat:owgu7nej6fgmbnokmy7bi4zslu

The American Presidency and the 25th Amendment in Contemporary TV Series: Fiction, Reality, and the Warped Mir

Monica Michlin
2012 TV Series  
Not only has actor Dennis Haysbert, 15 Read in particular Brian Stelter's article in The New York Times online, October 30, 2008: "Following the Script: Obama, McCain, and 'The West Wing'" (last consulted  ...  See this recent 2011 Pew Poll: http://peoplepress.org/2011/06/02/section-2-candidate-traits-and-experience/ See: "Feminist Fantasy: Geena Davis in the Oval Office", Alessandra Stanley, The New York  ... 
doi:10.4000/tvseries.1095 fatcat:hhmdr2uvvjhlpcrq3w6m3ljm2u

Abstracts from the Astrobiology Science Conference 2004

2004 International Journal of Astrobiology  
Phylogenetic analysis of chromosomal data Ward Wheeler, Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th St., New York, NY 10024-5192; wheeler@amnh.org Large-scale  ...  Astrobiology In Museums Martin Weiss, PhD, New York Hall of Science mweiss@nyscience.org; Acknowledgements We wish to thank The University of Hawaii and NASA-NAI (USA), PPARC (UK), and Osaka Vacuum (  ...  Mars Public and media reactions to 2003/2004 Mars missions: A case for the Internet as the primary source of astrobiology-related news Carol A.  ... 
doi:10.1017/s1473550404001648 fatcat:7edgphlk6bb2xf73w3rrb2vc3m

MiR-9 is involved in TGF-β1-induced lung cancer cell invasion and adhesion by targeting SOX7

Lichun Han, Wei Wang, Wei Ding, Lijian Zhang
2017 Journal of Cellular and Molecular Medicine  
Moreover, SOX7 expression was negatively correlated with miR-9 expression. miR-9 knockdown or SOX7 overexpression could suppress TGF-b1-induced NSCLC cell invasion and adhesion. miR-9 directly targets  ...  MicroRNA (miR)-9 plays different roles in different cancer types.  ...  Cell invasion assay The transwell inserts with 8-lm pore polycarbonate membrane (Corning, New York, NY, USA) were pre-coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). 1 9 10 5 cells in serum-free  ... 
doi:10.1111/jcmm.13120 pmid:28266181 pmcid:PMC5571535 fatcat:xxuczb5pqfbbfnuj6v44ypbxwq
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