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Characterization of Recombinant Mannan-Binding Lectin-Associated Serine Protease (MASP)-3 Suggests an Activation Mechanism Different from That of MASP-1 and MASP-2

S. Zundel, S. Cseh, M. Lacroix, M. R. Dahl, M. Matsushita, J.-P. Andrieu, W. J. Schwaeble, J. C. Jensenius, T. Fujita, G. J. Arlaud, N. M. Thielens
2004 Journal of Immunology  
Activation was prevented in the presence of protease inhibitors iodoacetamide and 1,10-phenanthroline but was not abolished upon substitution of Ala for the active site Ser 645 of MASP-3, indicating extrinsic  ...  Ultracentrifugation analysis indicated that rMASP-3 sedimented as a homodimer (s 20,w ‫؍‬ 6.2 ؎ 0.1 S) in the presence of Ca 2؉ , and as a monomer (s 20,w ‫؍‬ 4.6 ؎ 0.1 S) in EDTA.  ...  Acknowledgments We thank Christine Ebel for her help and advice with analytical ultracentrifugation and Bernard Dublet for performing mass spectrometry analyses.  ... 
doi:10.4049/jimmunol.172.7.4342 pmid:15034049 fatcat:aettabtjovcbdjkgpj2etov7oy

MASP-1 and MASP-3 Bind Directly to Aspergillus fumigatus and Promote Complement Activation and Phagocytosis

Anne Rosbjerg, Reinhard Würzner, Peter Garred, Mikkel-Ole Skjoedt
2021 Journal of Innate Immunity  
We have encountered an unknown pathway for complement activation and found that MASP-1 and MASP-3 have dual functions as enzymes and as PRMs.  ...  Among these were <i>Aspergillus fumigatus</i> – a dangerous pathogen, especially for immunocompromised patients.  ...  Acknowledgements The authors thank Jytte Bryde Clausen for technical assistance regarding the production of recombinant proteins and Katrine Pilely for assisting with the microscopy.  ... 
doi:10.1159/000514546 pmid:33780946 pmcid:PMC8460983 fatcat:lwreu7wa7bamzc77q4gvtcabqq

Herpes simplex encephalitis in adult patients with MASP-2 deficiency

Stéphanie Bibert, Jocelyne Piret, Mathieu Quinodoz, Emilie Collinet, Vincent Zoete, Olivier Michielin, Rafik Menasria, Pascal Meylan, Titus Bihl, Véronique Erard, Florence Fellmann, Carlo Rivolta (+3 others)
2019 PLoS Pathogens  
In a murine model of HSE, animals deficient in mannose binding lectins (MBL, the main pattern recognition molecule associated with MASP-2) had a decreased survival rate and an increased brain burden of  ...  Both MASP2 variants induced functional defects in vitro, including a reduced (R203W) or abolished (G634R) protein secretion, a lost capability to cleave MASP-2 precursor into its active form (G634R) and  ...  Michel Cuendet for providing template GROMACS input files and Julie-Christine Levesque from Luminex technology platform of Research Center of the CHU of Quebec-Laval University for assistance in Bio-Plex  ... 
doi:10.1371/journal.ppat.1008168 pmid:31869396 pmcid:PMC6944389 fatcat:cmopixzfenbifcqzqjlk277owi

Origin of Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 and MASP-3 Involved in the Lectin Complement Pathway Traced Back to the Invertebrate, Amphioxus

Y. Endo, M. Nonaka, H. Saiga, Y. Kakinuma, A. Matsushita, M. Takahashi, M. Matsushita, T. Fujita
2003 Journal of Immunology  
Kanno for excellent technical assistance.  ...  Zhang, Institute of Oceanology, Chinese Academy of Sciences for kindly giving us the amphioxus notochord cDNA library and amphioxus genomic DNA, respectively. We also thank K.  ...  W software (24) .  ... 
doi:10.4049/jimmunol.170.9.4701 pmid:12707349 fatcat:6u4atj5n2bekzm5auqosms4zua

Quantitative Characterization of the Activation Steps of Mannan-binding Lectin (MBL)-associated Serine Proteases (MASPs) Points to the Central Role of MASP-1 in the Initiation of the Complement Lectin Pathway

Márton Megyeri, Veronika Harmat, Balázs Major, Ádám Végh, Júlia Balczer, Dávid Héja, Katalin Szilágyi, Dániel Datz, Gábor Pál, Péter Závodszky, Péter Gál, József Dobó
2013 Journal of Biological Chemistry  
Based on the rate constants a kinetic model of lectin pathway activation was outlined.  ...  In summary, autoactivation of MASP-1 is crucial for the activation of MBL/ficolin-MASP complexes, and in the proenzymic phase zymogen MASP-1 controls the process. http://www.jbc.org/cgi/  ...  Hence, the autoactivation process can be modeled using recombinant fragments, and a stable zymogen form of these enzymes can be obtained by mutating a specific Arg to Gln.  ... 
doi:10.1074/jbc.m112.446500 pmid:23386610 pmcid:PMC3610966 fatcat:prnsaa77ozec3mmvfqvxivrvfu

MASP-1 of the complement system enhances clot formation in a microvascular whole blood flow model

Lorenz Jenny, József Dobó, Péter Gál, Gábor Pál, Wilbur A. Lam, Verena Schroeder, Robert B Sim
2018 PLoS ONE  
Here we describe the role of MASP-1 and complement activation in fibrin clot formation in a microvascular, whole blood flow model.  ...  We show for the first time a strong influence of MASP-1, complement activation and pathway-specific inhibition on coagulation in a microvascular flow system that is closest to human physiology, further  ...  microvascular flow model.  ... 
doi:10.1371/journal.pone.0191292 pmid:29324883 pmcid:PMC5764403 fatcat:dhnbwckr3zcovdz7x4562auaxm

The Serine Protease Domain of MASP-3: Enzymatic Properties and Crystal Structure in Complex with Ecotin

Christine Gaboriaud, Rajesh Kumar Gupta, Lydie Martin, Monique Lacroix, Laurence Serre, Florence Teillet, Gérard J. Arlaud, Véronique Rossi, Nicole M. Thielens, Claudio M. Soares
2013 PLoS ONE  
recently, revealing an unexpected important role of MASP-3 in early developmental processes.  ...  A tetrameric complex comprising an ecotin dimer and two MASP-3 SP domains was isolated and its crystal structure was solved and refined to 3.2 Å.  ...  We thank Sandor Cseh for construction of the pNT-Bac vector containing the MASP-3 ap-SP segment, Ludovic Lenclume for production of PAR1 and Evelyne Gout for baculovirus amplification and insect cells  ... 
doi:10.1371/journal.pone.0067962 pmid:23861840 pmcid:PMC3701661 fatcat:yjjs4nzhjbhy7ozwzmk6abj6fi

Lectin Pathway Enzyme MASP-2 and Downstream Complement Activation in COVID-19

Maximilian Peter Götz, Mikkel-Ole Skjoedt, Rafael Bayarri-Olmos, Cecilie Bo Hansen, Laura Pérez-Alós, Ida Jarlhelt, Thomas Benfield, Anne Rosbjerg, Peter Garred
2022 Journal of Innate Immunity  
To study a possible association between MASP-2 and COVID-19, we aimed at developing a sensitive and reliable MASP-2 ELISA.  ...  Overall, we developed a specific quantitative MASP-2 sandwich ELISA.  ...  Acknowledgments We thank Sif Kaas Nielsen, Emilie Bøgestad, Victoria Larsen, Mads Knudsen, and Bettina Eide Holm for excellent technical assistance and Kristina Dvončova for statistical advice.  ... 
doi:10.1159/000525508 pmid:35816998 fatcat:a6wbzyxxabcznbb5ocl3dolfui

Impact of MBL and MASP-2 gene polymorphism and its interaction on susceptibility to tuberculosis

Mengshi Chen, Ying Liang, Wufei Li, Mian Wang, Li Hu, Benjamin Kwaku Abuaku, Xin Huang, Hongzhuan Tan, Shi Wu Wen
2015 BMC Infectious Diseases  
Method: A total of 503 patients with TB and 419 healthy controls were recruited to participate in this case-control study.  ...  Unconditional logistic regression analysis was used to identify association between the various factors and TB whilst Marginal Structural Linear Odds Models were used to estimate the interactions.  ...  Acknowledgements This investigation was supported by Fundamental Research Funds for the Central Universities of Central South University and Global Foundation for China's Implementation Research in TB  ... 
doi:10.1186/s12879-015-0879-y pmid:25887173 pmcid:PMC4399571 fatcat:2rowpueeebhmnetanv7qj7nr4u

Establishing EGFP Congenic Mice in a NOD/Shi-scid IL2Rgnull (NOG) Genetic Background Using a Marker-Assisted Selection Protocol (MASP)

Hiroshi SUEMIZU, Chie YAGIHASHI, Tomoko MIZUSHIMA, Tomoyuki OGURA, Tomoo ETOH, Kenji KAWAI, Mamoru ITO
2008 Experimental animals  
a marker-assisted selection protocol (MASP).  ...  These results suggest that the NOG-EGFP mice, which were generated using the congenic method with MASP, acquired the immunological properties of the NOG mice.  ...  The electrophoresis data were processed using GeneMapper 4.0 software (Applied Biosystems).  ... 
doi:10.1538/expanim.57.471 pmid:18946184 fatcat:aoflvgukebh6bfjmkwem5nzlzu

Leprosy Association with Low MASP-2 Levels Generated by MASP2 Haplotypes and Polymorphisms Flanking MAp19 Exon 5

Angelica Beate Winter Boldt, Isabela Goeldner, Ewalda R. S. Stahlke, Steffen Thiel, Jens Christian Jensenius, Iara José Taborda de Messias-Reason, Masaru Katoh
2013 PLoS ONE  
Results: Two polymorphisms flanking exon 5 of MASP2 were associated with a dominant effect on high MASP-2 levels and an additive effect on low MAp19 levels.  ...  The *1C2-l [AG] haplotype, which harbors p.126L and the deficiency-causing p.439H variant, has a dominant effect on the susceptibility to the disease (P = 0.007, OR = 4.15).  ...  Nisihara for excellent technical assistance in performing ELISA assays. Contributed reagents/materials/analysis tools: ERS JCJ ST IJTMR. Wrote the paper: ABWB.  ... 
doi:10.1371/journal.pone.0069054 pmid:23935922 pmcid:PMC3728295 fatcat:6taeuk2f7ng43arjozwvyj33t4

The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles

Luis Miguel De Pablos, Isabel María Díaz Lozano, Maria Isabel Jercic, Markela Quinzada, Maria José Giménez, Eva Calabuig, Ana Margarita Espino, Alejandro Gabriel Schijman, Inés Zulantay, Werner Apt, Antonio Osuna
2016 Scientific Reports  
The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite's genome and plays a key role in immune evasion.  ...  A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide  ...  We thank Dr Silvia Longhi (LabMECh, INGEBI-CONICET) for her assistance with the serum samples from chronic Chagas disease patient.  ... 
doi:10.1038/srep27293 pmid:27270330 pmcid:PMC4897614 fatcat:v57a5cvnbjfizfais6dg5kutmq

Plasma MASP-1 concentration and its relationship to recovery from coronary artery lesion in children with Kawasaki disease

Rui-Xia Song, Qing-Mei Zou, Xiao-Hui Li, Nan-Ping Xu, Ting Zhang, Jin Fu, Xiao-Dai Cui
2015 Pediatric Research  
At 3-mo follow-up, there was a positive correlation between plasma MASP-1 concentration and recovery time of the patients with CAL older than 1 y. conclusion: Plasma MASP-1 concentration at the early stage  ...  Plasma Concentrations of MASP-1, C5b-9, and CD59 for Each Group The plasma MASP-1 concentration was significantly lower in the CAL group than that of healthy control and febrile control  ...  ACKNOWLEDGMENT We thank the families of the children for their active participation in this study. STATEMENT OF FINANCIAL SUPPORT No financial assistance was received to support this study.  ... 
doi:10.1038/pr.2015.223 pmid:26536449 fatcat:jxogahyevbau7obagrrezga4aq

CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity

Mo-fei Li, Jun Li, Li Sun
2016 Scientific Reports  
These results indicate for the first time that a teleost MAP acts one hand as a regulator that promotes the lectin pathway of complement activation via its ability to recruit MBL to MASP, and other hand  ...  as a modulator of immune cell activity.  ...  activation process.  ... 
doi:10.1038/srep39287 pmid:28008939 pmcid:PMC5180248 fatcat:j7epfke47jbqtgfgyiwfhxqvn4

MASP-1 Induces a Unique Cytokine Pattern in Endothelial Cells: A Novel Link between Complement System and Neutrophil Granulocytes

Péter K. Jani, Erika Kajdácsi, Márton Megyeri, József Dobó, Zoltán Doleschall, Krisztina Futosi, Csaba I. Tímár, Attila Mócsai, Veronika Makó, Péter Gál, László Cervenak, Ger van Zandbergen
2014 PLoS ONE  
Citation: Jani PK, Kajdácsi E, Megyeri M, Dobó J, Doleschall Z, et al. (2014) MASP-1 Induces a Unique Cytokine Pattern in Endothelial Cells: A Novel Link between Complement System and Neutrophil Granulocytes  ...  We have previously shown that mannan-binding lectin-associated serine protease-1 (MASP-1), the most abundant enzyme of the complement lectin pathway, can induce p38-MAPK activation, NFkappaB signaling,  ...  Simon and Anna Tóth for expert technical assistance with the neutrophil chemotaxis assays at Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary.  ... 
doi:10.1371/journal.pone.0087104 pmid:24489848 pmcid:PMC3906121 fatcat:5emorhdg2za7vhfpbbl4sdoeja
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