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Linear and Non-Linear Middle Infrared Spectra of Penicillin G in the CO Stretching Mode Region

Elena Ragnoni, Sara Catalini, Maurizio Becucci, Andrea Lapini, Paolo Foggi
2021 Symmetry  
In order to better characterize the spectral IR features in the CO stretching region, broadband middle infrared pump-probe spectra are recorded.  ...  In this work we report the linear and non-linear IR spectral response characterization of the CO bonds of PenicillinG sodium salt in D2O and in DMSO−d6 solutions.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/sym13010106 fatcat:zscc5tub7vb25jygr2lnlqt7y4

Distinguishing nuclei-specific benzo[a]pyrene-induced effects from whole-cell alterations in MCF-7 cells using Fourier-transform infrared spectroscopy

Blessing E. Obinaju, Nigel J. Fullwood, Francis L. Martin
2015 Toxicology  
Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes.  ...  B[a]P-induced effects in whole-cells included alterations to lipids, DNA and protein spectral regions.  ...  Finally, significant correlation between the peak areas for intact cells and isolated nuclei may imply that the protein/DNA regions, as well as alterations to these regions in IR spectra of whole cells  ... 
doi:10.1016/j.tox.2015.07.001 pmid:26148868 fatcat:vjumjtsx3vc43cybwou5ckqrdq

Correlation of p16INK4A expression and HPV copy number with cellular FTIR spectroscopic signatures of cervical cancer cells

Kamila M. Ostrowska, Amaya Garcia, Aidan D. Meade, Alison Malkin, Ifeoluwapo Okewumi, John J. O'Leary, Cara Martin, Hugh J. Byrne, Fiona M. Lyng
2011 The Analyst  
Principal Component Analysis (PCA) of the infrared absorption spectra revealed differences between nuclear and cytoplasmic spectroscopic signatures for all cell lines, and furthermore clearly differentiated  ...  the groups of spectra representing each cell line.  ...  Acknowledgements This work was funded by the Department of Education and Science Technological Sector Research Strand III programme and enabled through the National Biophotonics and Imaging Platform, Ireland  ... 
doi:10.1039/c0an00910e pmid:21290054 fatcat:iqwsey4wsndvvneveudglwlbse

Study of the intracellular nanoparticle-based radiosensitization mechanisms in F98 glioma cells treated with charged particle therapy through synchrotron-based infrared microspectroscopy

I. Martínez-Rovira, O. Seksek, I. Dokic, S. Brons, A. Abdollahi, I. Yousef
2020 The Analyst  
This work provides new insights into the molecular changes in response to nanoparticle-based radiotherapy treatments using ion beams through synchrotron-based infrared microspectroscopy.  ...  Acknowledgements The authors acknowledge the granted beamtime at ALBA-CELLS Synchrotron.  ...  The infrared spectra were collected using the transmission operation mode of the FTIR microscope.  ... 
doi:10.1039/c9an02350j pmid:31993615 fatcat:asbutkm6orferoxinkxbujvoui

Embryonic stem cell differentiation studied by FT-IR spectroscopy

Diletta Ami, Tui Neri, Antonino Natalello, Paolo Mereghetti, Silvia Maria Doglia, Mario Zanoni, Maurizio Zuccotti, Silvia Garagna, Carlo Alberto Redi
2008 BBA - Molecular Cell Research  
Between days 4 to 7 of differentiation, these spectral changes in the protein amide I band (1700-1600 cm − 1 ) and in the nucleic acid absorption region (1050-850 cm − 1 ) indicated that mRNA translation  ...  Principal component analysis and subsequent linear discriminant analysis enabled us to segregate stem cell spectra into separate clusterscorresponding to different differentiation timesand to identify  ...  Acknowledgements We are grateful to Astrid Gräslund (Stockholm University) for reading the manuscript and to Luca De Gioia (University  ... 
doi:10.1016/j.bbamcr.2007.08.003 pmid:17916392 fatcat:lphusm2iszf5tnv3tk3k6e2svy

High spatial resolution (1.1 μm and 20 nm) FTIR polarization contrast imaging reveals pre-rupture disorder in damaged tendon

Richard Wiens, Catherine R. Findlay, Samuel G. Baldwin, Laurent Kreplak, J. Michael Lee, Samuel P. Veres, Kathleen M. Gough
2016 Faraday discussions  
The results and possibilities for the application of these high-spatial-resolution FTIR techniques in spectral pathology, and eventually in clinical applications, are discussed.  ...  Our goal is to explore the changes induced in tendon after the application of damaging mechanical stress, and the consequences for the healing processes.  ...  FTIR PCI at 90 showed (C) a high degree of molecular alignment with fibrils in the case of uniform stretch and (D) alternating regions of alignment and crimp in the case of non-uniform stretch.  ... 
doi:10.1039/c5fd00168d pmid:27048856 fatcat:6boemxi4ozdxhijkoeambuetgy

Large amplitude inversion tunneling motion in ammonia, methylamine, hydrazine, and secondary amines: From structure determination to coordination chemistry

Ha Vinh Lam Nguyen, Iwona Gulaczyk, Marek Kręglewski, Isabelle Kleiner
2021 Coordination chemistry reviews  
Regarding the spectrum of hydrazine, the vibrational ground state lies in the microwave region.  ...  its group theoretical considerations, theoretical studies, electronic ground state spectra in the infrared range, and its coordination complexes, especially those formed with calcium and copper.  ...  In newer contributions, the high resolution infrared spectrum of methylamine has been studied in the regions of the C-H stretching [173, 174] and NH 2 scissors modes [175] .  ... 
doi:10.1016/j.ccr.2021.213797 fatcat:wtxzl6kr5vbsbjnf5px42bucci

Multicolored stain-free histopathology with coherent Raman imaging

Christian W Freudiger, Rolf Pfannl, Daniel A Orringer, Brian G Saar, Minbiao Ji, Qing Zeng, Linda Ottoboni, Wei Ying, Christian Waeber, John R Sims, Philip L De Jager, Oren Sagher (+5 others)
2012 Laboratory Investigation  
Here we describe the development of an alternative, multicolored way to visualize tissue in real time through the use of coherent Raman imaging (CRI), without the use of dyes.  ...  However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system.  ...  John Sims for the mouse stroke model, Chrissy Rooney for the invasive glioma model, Dr. Fa-Ke Lu and Srinjan Basu for the cell images, and Dr. Anita Huettner for early pathology work.  ... 
doi:10.1038/labinvest.2012.109 pmid:22906986 pmcid:PMC3622202 fatcat:xebk57j2lvcahemqj4v67ltdja

Erratum: Multicolored stain-free histopathology with coherent Raman imaging

Christian W Freudiger, Rolf Pfannl, Daniel A Orringer, Brian G Saar, Minbiao Ji, Qing Zeng, Linda Ottoboni, Wei Ying, Christian Waeber, John R Sims, Philip L De Jager, Oren Sagher (+5 others)
2012 Laboratory Investigation  
Here we describe the development of an alternative, multicolored way to visualize tissue in real time through the use of coherent Raman imaging (CRI), without the use of dyes.  ...  However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system.  ...  John Sims for the mouse stroke model, Chrissy Rooney for the invasive glioma model, Dr. Fa-Ke Lu and Srinjan Basu for the cell images, and Dr. Anita Huettner for early pathology work.  ... 
doi:10.1038/labinvest.2012.139 fatcat:syxdub33k5c7zjovyjvo7blbqi

Spider Toxin and Raventoxin Time–Resolved Absorption and Resonance FT–IR and Raman Biospectroscopy and Density Functional Theory (DFT) Investigation of Vibronic–Mode Coupling Structure in Vibrational Spectra Analysis

Alireza Heidari, Jennifer Esposito, Angela Caissutti
2019 Zenodo  
Vibration modes of methylene, carboxyl acid and phenyl cycle are separately investigated. The obtained values confirm high accuracy and validity of results obtained from calculations.  ...  levels of F/6–31G*, HF/6–31++G**, MP2/6–31G, MP2/6–31++G**, BLYP/6–31G, BLYP/6–31++G**, B3LYP/6–31G and B3LYP6–31–HEG**.  ...  In the current paper, stretching vibration of carbonyl mode is at 2197 cm -1 which that is a mid-range value.  ... 
doi:10.5281/zenodo.3870727 fatcat:c2wsqudj3bfa5fkpdmbokn4ifu

Receptor-Meditated Endocytosis by Hyaluronic Acid@Superparamagnetic Nanovetor for Targeting of CD44-Overexpressing Tumor Cells

Kwang Yu, Meng Lin, Hyun-Ju Lee, Ki-Sik Tae, Bo-Sun Kang, Je Lee, Nam Lee, Young Jeong, Seung-Yun Han, Do Kim
2016 Nanomaterials  
Fourier transform infrared (FTIR) and zeta-potential studies confirmed the chemical bonding between amino acid linker and polysaccharides. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (  ...  The synthesis of HA-Glutamic Acid (GA)@SPIONs was included oxidization of nanoparticle's surface with H 2 O 2 followed by activation of hydroxyl group and reacting glutamic acid as an intermediate molecule  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/nano6080149 pmid:28335277 pmcid:PMC5224623 fatcat:3tqcd3g37nezlg4n7wveftjfua

Different Raman spectral patterns of primary rat pancreatic β cells and insulinoma cells

Qiu-Li Zhou, Xi Rong, Fang Wei, Rui-Qiong Luo, Hong Liu
2015 Journal of Biomedical Optics  
The details of the optical heterogeneity between these two populations were determined based on different Raman regions primarily involving nucleic acid and protein contents, which are the most distinct  ...  The results of this work indicate that Raman spectroscopy is a promising tool for the noninvasive and label-free differentiation of insulinoma cells and normal pancreatic β cells.  ...  We thank the Laboratory of Biophysics of Guangxi Academy of Sciences for providing the laboratory apparatus, including the Raman spectroscopy setup.  ... 
doi:10.1117/1.jbo.20.4.047001 pmid:25848805 fatcat:gqhiqhdrundnresvwb7mmrnesa

Biomimetic gradient scaffold of collagen–hydroxyapatite for osteochondral regeneration

Cristian Parisi, Luca Salvatore, Lorenzo Veschini, Maria Paola Serra, Carl Hobbs, Marta Madaghiele, Alessandro Sannino, Lucy Di Silvio
2020 Journal of Tissue Engineering  
in different sub-regions of the scaffold, according to their high chondrogenic or osteogenic characteristics.  ...  In this study, a novel gradient scaffold has been fabricated, implementing a gradient structure in the design to mimic the anatomical, biological and physicochemical properties of bone and cartilage as  ...  collagen fibres and HAp was given by the detection of the characteristic peak of carbonate stretching at 874 cm −1 (ν 2 CO 3 ) in the spectrum of the bone-like region of the gradient scaffold (Figure  ... 
doi:10.1177/2041731419896068 pmid:35003613 pmcid:PMC8738858 fatcat:zg6ml62gz5a4lpyjroue7zwb4y

FTIR-spectroscopic and LA-ICP-MS imaging for combined hyperspectral image analysis of tumor models

A. Balbekova, M. Bonta, S. Török, J. Ofner, B. Döme, A. Limbeck, B. Lendl
2017 Analytical Methods  
Advantages of combined image analysis were demonstrated using infrared spectroscopy and mass spectrometry while characterizing biochemical changes occurring in tumor models.  ...  spectra of the amide I IR spectral region (C), and mean raw IR spectrum of the spectral region of the band assigned to phosphate symmetric stretching vibrational mode (D).  ...  spectra of the amide I IR spectral region (C), and mean raw IR spectrum of the spectral region of the band assigned to phosphate symmetric stretching vibrational mode (D).  ... 
doi:10.1039/c7ay01369h fatcat:x3rcb3dan5hrrhcf5eoykpwwfu

Fabrication of naturel pumice/hydroxyapatite composite for biomedical engineering

Baran Komur, Tim Lohse, Hatice Merve Can, Gulnar Khalilova, Zeynep Nur Geçimli, Mehmet Onur Aydoğdu, Cevriye Kalkandelen, George E. Stan, Yesim Muge Sahin, Ahmed Zeki Sengil, Mediha Suleymanoglu, Serap Erdem Kuruca (+5 others)
2016 BioMedical Engineering OnLine  
In the world, the lifetime risk of a bone fracture is 40-50 % for women and 13-22 % for men [2] .  ...  Results: Variances in the sintering temperature (for 5 wt% NP composites) between 1000 and 1300 °C, reveal about 700 % increase in the microhardness (~100 and 775 HV, respectively).  ...  GES is thankful for the financial support of the NIMP PN4501-3 Core Programme. Competing interests The authors declare that they have no competing interests.  ... 
doi:10.1186/s12938-016-0203-0 pmid:27388324 pmcid:PMC4937607 fatcat:ukh6kznmdfb6bm3xcxpgy2v5c4
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