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Light field microscopy

Marc Levoy, Ren Ng, Andrew Adams, Matthew Footer, Mark Horowitz
2006 ACM Transactions on Graphics  
Figure 1 : At left is a light field captured by photographing a speck of fluorescent crayon wax through a microscope objective and microlens array.  ...  We also show that synthetic focusing followed by 3D deconvolution is equivalent to applying limited-angle tomography directly to the 4D light field.  ...  Acknowledgments We thank Gordon Kino, Olav Solgaard, and Joseph Goodman for advice on optics, Julie Theriot, Stephen Smith, and Rudolf Oldenbourg for advice on microscopy and applications, David Biggs  ... 
doi:10.1145/1141911.1141976 fatcat:nlzqwc5jdbazpdimradftusbge

Light field microscopy

Marc Levoy, Ren Ng, Andrew Adams, Matthew Footer, Mark Horowitz
2006 ACM SIGGRAPH 2006 Papers on - SIGGRAPH '06  
Figure 1 : At left is a light field captured by photographing a speck of fluorescent crayon wax through a microscope objective and microlens array.  ...  We also show that synthetic focusing followed by 3D deconvolution is equivalent to applying limited-angle tomography directly to the 4D light field.  ...  Acknowledgments We thank Gordon Kino, Olav Solgaard, and Joseph Goodman for advice on optics, Julie Theriot, Stephen Smith, and Rudolf Oldenbourg for advice on microscopy and applications, David Biggs  ... 
doi:10.1145/1179352.1141976 fatcat:tg3doyyiq5empn5j32oy7uup24

Fourier light-field microscopy

Changliang Guo, Wenhao Liu, Xuanwen Hua, Haoyu Li, Shu Jia
2019 Optics Express  
Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric imaging ranging from single-cell specimens to the mammalian brain.  ...  To address the challenge, in this work, we report Fourier LFM (FLFM), a system that processes the light-field information through the Fourier domain.  ...  Here, we introduce Fourier light-field microscopy (FLFM) to achieve high-quality imaging and rapid light-field reconstruction.  ... 
doi:10.1364/oe.27.025573 pmid:31510428 pmcid:PMC6825611 fatcat:mui37iafhvhbriqh77zphipjru

Light-Field Microscopy with a Consumer Light-Field Camera

Lois Mignard-Debise, Ivo Ihrke
2015 2015 International Conference on 3D Vision  
We analyze the new virtual imaging mode and use it in two different setups for implementing light-field microscopy using a Lytro camera.  ...  We explore the use of inexpensive consumer lightfield camera technology for the purpose of light-field microscopy. Our experiments are based on the Lytro (first generation) camera.  ...  Light-field microscopy was introduced by Levoy et al. [9] and later augmented with light-field illumination [10] .  ... 
doi:10.1109/3dv.2015.45 dblp:conf/3dim/Mignard-DebiseI15 fatcat:e6dk4eevpfdshnsm2g3jws4cti

Light-field Microscopy with a Consumer Light-field Camera [article]

Lois Mignard-Debise, Ivo Ihrke
2015 arXiv   pre-print
We analyze the new vir- tual imaging mode and use it in two different setups for im- plementing light-field microscopy using a Lytro camera.  ...  We explore the use of inexpensive consumer light- field camera technology for the purpose of light-field mi- croscopy. Our experiments are based on the Lytro (first gen- eration) camera.  ...  Light-field microscopy was introduced by Levoy et al. [9] and later augmented with light-field illumination [10] .  ... 
arXiv:1508.03590v2 fatcat:it3dh6uupfa5bgse72jqxqse3a

Single Molecule Light Field Microscopy [article]

Ruth R Sims, Sohaib Abdul Rehman, Martin O Lenz, Adam Clark, Edward W Sanders, Aleks Ponjavic, Leila Muresan, Steven F Lee, Kevin O'Holleran
2020 bioRxiv   pre-print
We introduce single molecule light field microscopy (SMLFM), a novel 3D single molecule localization technique that is capable of up to 20 nm isotropic precision across a 6 μm depth of field.  ...  Light field microscopy Light field microscopy (LFM) offers single-shot threedimensional imaging by simultaneously collecting light from a large, continuous depth-of-field.  ...  This configuration is known as Fourier light field microscopy (25) (26) (27) .  ... 
doi:10.1101/2020.05.20.104802 fatcat:xe4xfl3x45chbhulbo5lvnfute

Robust Depth Estimation for Light Field Microscopy

Luca Palmieri, Gabriele Scrofani, Nicolò Incardona, Genaro Saavedra, Manuel Martínez-Corral, Reinhard Koch
2019 Sensors  
Light field technologies have seen a rise in recent years and microscopy is a field where such technology has had a deep impact.  ...  In this work, a robust approach is proposed where accurate depth maps can be produced exploiting the information recorded in the light field, in particular, images produced with Fourier integral Microscope  ...  The main limitation of light field in microscopy is the spatial resolution [5] .  ... 
doi:10.3390/s19030500 fatcat:hq74aw2pkbfjrfovhcczqaen5q

Artifact-free deconvolution in light field microscopy

Anca Stefanoiu, Josue Page, Panagiotis Symvoulidis, Gil G. Westmeyer, Tobias Lasser
2019 Optics Express  
The sampling patterns of the light field microscope (LFM) are highly depth-dependent, which implies non-uniform recoverable lateral resolution across depth.  ...  Moreover, reconstructions using state-of-the-art approaches suffer from strong artifacts at axial ranges, where the LFM samples the light field at a coarse rate.  ...  Aliasing in light field microscopy The light field microscope A light field microscope (LFM) is built by placing a micro-lens array (MLA) into the optical path of a conventional wide-field microscope  ... 
doi:10.1364/oe.27.031644 fatcat:hlbarh7pjvdundecf63hyhyori

Quasi-spectral characterization of intracellular regions in bright-field light microscopy images [article]

Kirill Lonhus, Renata Rychtarikova, Ganna Platonova, Dalibor Stys
2020 arXiv   pre-print
Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement makes this method being state-of-the-art.  ...  The method is compatible with existent, ordinary bright-field microscopes and requires mathematically sophisticated data processing.  ...  Indeed, classical bright-field microscopy measures properties of incoming light affected by a sample.  ... 
arXiv:1908.03696v2 fatcat:osj6mjvajfdc5kq6qhl6m7uqpa

A Vignetting Model for Light Field Cameras with an Application to Light Field Microscopy

Lois Mignard-Debise, Ivo Ihrke
2019 IEEE Transactions on Computational Imaging  
A Vignetting Model for Light Field Cameras with an Application to Light Field Microscopy.  ...  to a custom-built light field microscope.  ...  INTRODUCTION & RELATED WORKS T HE field of light field imaging has seen the emergence of many different types of cameras to measure the light field of a scene.  ... 
doi:10.1109/tci.2019.2911856 fatcat:bequuraf7rflhnm75hagmjk254

Dual-dimensional microscopy: real-time in vivo three-dimensional observation method using high-resolution light-field microscopy and light-field display

Jonghyun Kim, Seokil Moon, Youngmo Jeong, Changwon Jang, Youngmin Kim, Byoungho Lee
2018 Journal of Biomedical Optics  
Dualdimensional microscopy: real-time in vivo three-dimensional observation method using highresolution light-field microscopy and light-field display," J. Abstract.  ...  Here, we present dual-dimensional microscopy that captures both two-dimensional (2-D) and lightfield images of an in-vivo sample simultaneously, synthesizes an upsampled light-field image in real time,  ...  Dual-Dimensional Microscopy and Light-Field Upsampling Figure 1 (a) shows the schematic diagram of a DDM setup.  ... 
doi:10.1117/1.jbo.23.6.066502 pmid:29931838 fatcat:poalef4l5jfpphetemvgnfoley

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data [article]

Ganna Platonova, Dalibor Stys, Pavel Soucek, Kirill Lonhus, Jan Valenta, Renata Rychtarikova
2021 arXiv   pre-print
The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy.  ...  At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped with a high-resolution (4872x3248) image  ...  In label-free bright-field microscopy, the signal is generated mainly by diffraction of a wide range of light wavelengths in the sample.  ... 
arXiv:1903.06519v4 fatcat:ifu2puiinbbnzbprakhj4ofawu

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light

J. Nylk, C. Mitchell, T. Vettenburg, F. J. Gunn-Moore, K. Dholakia, Thomas G. Brown, Carol J. Cogswell, Tony Wilson
2014 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI  
In this paper we explore wavefront shaping for the enhancement of light sheet microscopy with scattered light.  ...  We show experimental verification of this result, demonstrating the use of the propagation invariant Bessel beam to extend the field of view of a high resolution scattered light, light sheet microscope  ...  INTRODUCTION Light sheet microscopy (LSM) is a powerful, wide-field imaging method that has revolutionized fluorescence imaging in developmental biology and related disciplines [1] [2] [3] [4] .  ... 
doi:10.1117/12.2038525 fatcat:gtzjnrs35zhj5e5pivhwibj6gq

Fourier DiffuserScope: Single-shot 3D Fourier light field microscopy with a diffuser [article]

Fanglin Linda Liu, Grace Kuo, Nick Antipa, Kyrollos Yanny, Laura Waller
2020 arXiv   pre-print
Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample, without any moving parts.  ...  Unfortunately, the 3D capability of LFM comes with a significant loss of lateral resolution at the focal plane, which is highly undesirable in microscopy.  ...  System overview for Fourier DiffuserScope and Fourier light field microscopy (FLFM).  ... 
arXiv:2006.16343v1 fatcat:itesjsazgbdyhgfp6zu2ihgbny

Transformation of Liponsomes Revealed by Dark-field Light Microscopy

Hirokazu HOTANI
1987 MEMBRANE  
doi:10.5360/membrane.12.281 fatcat:75nlumgc6fatlm74iyt7ot5zpe
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