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Lei Wang: Expanding the language of life Once you've removed transfection effi ciency as a limiting factor, what limitations remain? ... While a graduate student in Peter Schultz's lab at UC Berkeley, Lei Wang figured out a way to stick unnatural amino acids into E. coli proteins at rare stop codons. ...doi:10.1084/jem.20513pi pmid:19103882 pmcid:PMC2605214 fatcat:udjng4r7ejgkvnzssse26hscpe
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus causing AHPND (VPAHPND) is the most serious disease affecting shrimp farming. The PirAvp and PirBvp toxins of VPAHPND are known virulence factors. However, the corresponding target protein in shrimp that mediates their action has not been identified. By screening yeast two-hybrid cDNA libraries from intestine, stomach, and hepatopancreas of Litopenaeus vannamei, the protein with the largest increase in genedoi:10.3389/fimmu.2022.940405 pmid:35860240 pmcid:PMC9289683 doaj:72b93607c1ba47aaa0a9e8dc1d500ff7 fatcat:prmg4jivf5cxvobl4pgne3muoa
more »... ion in shrimp hepatopancreas in response to VPAHPND challenge was identified and designated LvFABP. Analysis revealed high sequence homology of the LvFABP gene and a lipocalin/cytosolic fatty acid binding gene. Yeast two-hybrid pairwise analysis, GST-pull down assay, and far-western blot assay were performed to determine the interaction between LvFABP and PirBvp. LvFABP was able to directly bind to PirBvp. The expression of LvFABP in the hepatopancreas was significantly higher at P23 and P27 developmental stages of L. vannamei. RNA interference (RNAi) of LvFABP reduced the mortality, histopathological signs of AHPND in the hepatopancreas, and the number of virulent VPAHPND bacteria in the intestine, stomach, and hepatopancreas after VPAHPND challenge. We concluded that the LvFABP was involved in AHPND pathogenesis and acted as a VPAHPND toxin interacting protein. This is the first identification of VPAHPND toxin interacting protein from the shrimp digestive system by yeast two-hybrid library screening and were confirmed by in vitro protein interaction verification and in vivo challenge experiments. This study provides novel insight into the contributions of LvFABP towards AHPND pathogenesis in shrimp. The findings could inform AHPND preventative measures in shrimp farming.
Most of current ontology mapping methods can not treat different mapping tasks in different ways referred to the features of the input ontology. And they combine different features of ontology without full consideration of the influences on mapping results caused mapping features. In view of the above questions, this paper proposes mapping method which can use entropy decision-making method to determine the combined weight of the different features of ontology. Experiments show that this methoddoi:10.1016/j.phpro.2012.03.365 fatcat:poqnmp6gyjezvmewtjy3y6yzgu
more »... can maintain the stability and the commonality, and improve the recall ratio and the precision ratio at the same time
Rationale: Breast cancer is the most prevalent malignancy in women worldwide. Our patient presented with a history of breast liposarcoma (LPS) and was found to have pleural metastasis during the initial workup. Patient concerns: The patient was complaining about chest pain and dyspnea that had persisted for a week. Diagnoses: After a full evaluation and histological diagnosis, she was diagnosed as metastatic breast LPS. Interventions: We adopted 6 cycles of pegylated liposomal doxorubicin (PLD)doi:10.1097/md.0000000000007340 pmid:28682882 pmcid:PMC5502155 fatcat:tbcjmjij4bfexaharj2iwfyy4e
more »... plus ifosfamide as 1st-line palliative chemotherapy, combined with local pleural effusion management. Outcomes: The patient's symptoms were notably relieved, and both malignant metastatic lesions and pleural effusion were controlled. Lessons: Although metastatic breast LPS is rarely reported and incurable, more clinical experience and use of next-generation sequencing should be helpful in finding the effective treatment for metastatic LPS.
Because of its ability to study specifically labeled structures, fluorescence microscopy is the most widely used technique for investigating live cell dynamics and function. Fluorescence correlation spectroscopy is an established method for studying molecular transport and diffusion coefficients at a fixed spatial scale. We propose a new approach, dispersion-relation fluorescence spectroscopy (DFS), to study the transport dynamics over a broad range of spatial and temporal scales. The moleculesdoi:10.1103/physrevlett.109.188104 pmid:23215337 fatcat:477zlzz4nbcilcltjnshwp752u
more »... of interest are labeled with a fluorophore whose motion gives rise to spontaneous fluorescence intensity fluctuations that are analyzed to quantify the governing mass transport dynamics. These data are characterized by the effective dispersion relation. We report on experiments demonstrating that DFS can distinguish diffusive from advection motion in a model system, where we obtain quantitatively accurate values of both diffusivities and advection velocities. Because of its spatially resolved information, DFS can distinguish between directed and diffusive transport in living cells. Our data indicate that the fluorescently labeled actin cytoskeleton exhibits active transport motion along a direction parallel to the fibers and diffusive in the perpendicular direction.
The performances of the model were enough for further analysis (Wang et al., 2010) . ... The Chinese total annual catch accounting for 80% of global catches of O. bartramii in the Northwest Pacific Ocean (Wang et al., 2020) . ...doi:10.3389/fmars.2021.741620 doaj:08f3e13dd9724dbfabe3befe462e27aa fatcat:vyjxioxhwndyxmxulhh7py5wza
Acknowledgments: The authors are grateful to Michelle Gleeson (Hunter Medical Research Institute, University of Newcastle, Australia), Dan Wang (West China Hospital, Sichuan University, China), and Zhi ... (Gang Wang, email@example.com) and X.Z. conceived the study, performed the data interpretation and manuscript revision, and took accountability for all aspects of the work. ... (Gang Wang, firstname.lastname@example.org) interpreted the results and contributed to the manuscript revision. All authors have read and agreed to the published version of the manuscript. Funding ...doi:10.3390/nu14122525 pmid:35745259 pmcid:PMC9229860 fatcat:p36fsvuaojcqpa7ifrdwypi6ja
Xiaohui Wang from Institute of Metal Research, Chinese Academy of Sciences, for their helpful discussions. ... wrote Sections 5.1 and 5.2; Lei Su wrote Section 6.4; Lei Miao wrote Section 5.8; Guojun Zhang wrote Sections 4.6 and 5.4; Yiguang Wang wrote Section 4.5; Xiwei Qi wrote Sections 4.8 and 4.9; Lei Yao ... Recently, Wang et al. ...doi:10.1007/s40145-021-0477-y fatcat:vs3dwnclcfbh7eacobzr6kgt4q
Learning discriminative image representations plays a vital role in long-tailed image classification because it can ease the classifier learning in imbalanced cases. Given the promising performance contrastive learning has shown recently in representation learning, in this work, we explore effective supervised contrastive learning strategies and tailor them to learn better image representations from imbalanced data in order to boost the classification accuracy thereon. Specifically, we proposearXiv:2103.14267v1 fatcat:n733ddutxnevrljf3uqp3whueq
more »... novel hybrid network structure being composed of a supervised contrastive loss to learn image representations and a cross-entropy loss to learn classifiers, where the learning is progressively transited from feature learning to the classifier learning to embody the idea that better features make better classifiers. We explore two variants of contrastive loss for feature learning, which vary in the forms but share a common idea of pulling the samples from the same class together in the normalized embedding space and pushing the samples from different classes apart. One of them is the recently proposed supervised contrastive (SC) loss, which is designed on top of the state-of-the-art unsupervised contrastive loss by incorporating positive samples from the same class. The other is a prototypical supervised contrastive (PSC) learning strategy which addresses the intensive memory consumption in standard SC loss and thus shows more promise under limited memory budget. Extensive experiments on three long-tailed classification datasets demonstrate the advantage of the proposed contrastive learning based hybrid networks in long-tailed classification.
Wang R, Lei L, Wang Y, Levine A, Popescu G. Dispersion relation second time scale. Biophys Chem. 1993;48(2):221–239. fluorescence spectroscopy. ... 1,2 Abstract: While it is well known that the cytoskeleton plays a fundamental role in maintaining Lei Lei 3 cell shape, performing cell ...doi:10.2147/chc.s62865 fatcat:kv3zlu5qf5cwzoxj2si3xvdn6a
It is well known that bees cluster together in cold weather, in the process of swarming (when the "old" queen leaves with part of the colony) or absconding (when the queen leaves with all the colony) and in defense against intruders such as wasps or hornets. In this paper we describe a fairly different clustering process which occurs at any temperature and independently of any special stimulus or circumstance. As a matter of fact, this process is about four times faster at 28 degree CelsiusarXiv:1112.5526v1 fatcat:xv3oo7zn7zcstdre6by7g3xzbi
more »... at 15 degrees. Because of its simplicity and low level of "noise" we think that this phenomenon can provide a means for exploring the strength of inter-individual attraction between bees or other living organisms. For instance, and at first sight fairly surprisingly, our observations showed that this attraction does also exist between bees belonging to different colonies. As this study is aimed at providing a comparative perspective, we also describe a similar clustering experiment for red fire ants.
Gradient-index dielectric lenses are generated based on the coordinate transformation by compressing the homogeneous air spaces quasi-conformally towards and outwards the primary source. The threedimensional modeling is then performed through revolving the prescribed transformational media 180 degrees around the focal point to reach the architecture of barrel-vaults. It is found that all these twoand three-dimensional transformation-optical designs are capable of producing fan-beams efficientlydoi:10.1038/srep20530 pmid:26847048 pmcid:PMC4742821 fatcat:lusmpc2pjzbulc327lzlxeolmq
more »... over a broad frequency range with their main lobes possessing the narrow beamwidth in one dimension and the wide beamwidth in the other, while having the great ability of the wide angular scanning. Finally, we propose to construct such four types of fan-beam lenses through multiple-layered dielectrics with non-uniformed perforations and experimentally demonstrate their excellent performances in the fan-beam synthesis. With simultaneously high gain and wide azimuth coverage, fan-beam radiator has been widely adopted in various applications among different disciplines 1 . It demonstrates highly efficiency for the bone scan in the medical imaging 2 , yields a significant link-budget advantage for the indoor wireless communication 3 , and also presents superior qualities for the height-finding as well as the obstacle detection in the radar system 4-6 . The truncated parabolic reflector (TPR) has been so far the most commonly used device to produce the fan-beams 7 . Due to the inequality of the radiating aperture, the primary spherical waves from the focus are collimated differently. The narrower beam-width is achieved in the dimension where the reflector has the intact radiating aperture and the wider beam-width is obtained in the other dimension. The dielectric lens, at the same time, has also been proved to be a promising candidate to synthesize the fan-beams. Different from the three-dimensional (3D) construction of the TPR, most of the lens designs only adopt two-dimensional (2D) structures, such as the cylindrical Luneburg lenses 8-10 , and the up-to-date meta-surface lenses 11-14 , serving in the parallel plate waveguides or on the grounded metamaterial surfaces. These demonstrations successfully fulfill the functionality as fan-beam generators to collimate the radiation in the lens locating plane and maintain the beam pattern nearly unchanged in others, possessing the merits of low profile and convenience in the conformal design. However, such 2D proposals of the thin sheets also constrain their application at the same time due to the structural limitation of small perception windows connecting the outside in the direction of the main beam. Therefore, the practical utilization of the dielectric lenses compared with the ubiquitous TPRs in the radar antenna system for the fan-beam applications is still limited. Transformation optics 15-17 , as a promising strategy to control the electromagnetic fields since 2006, has been widely adopted to build up the new antenna system 18-24 . These proposals, either based on the 2D coordinate transformation to synthesize the flat lenses, or creating the 3D construction of the transformational media by rotating the 2D permittivity map around its symmetry axis 25 , have demonstrated the great efficiency to collimate the cylindrical wave from a primary line source or the spherical wave from an isotropic point source. Although 2D lenses should be considered as the valid candidate for fan-beam lenses, the existing literatures have scarcely mentioned the synthesis of fan-beams using the transformation optics, with most of the 2D designs focusing on the perfect generation of the directive radiations, and the 3D set-ups aiming for the sharp pencil-beams. Transformation optics, having much wider applications in the wave control, should be powerful for different kinds of beam-forming and function as a very useful tool for the design of fan-beams. Based on these considerations, we demonstrate the transformational constructions of the 2D and the 3D lenses for the fan-beam generation in this paper. The homogeneous air spaces are compressed quasi-conformally based on the 2D coordinate transformation to come up with two kinds of the gradient-index dielectric lenses having the plano-concave profile and flat profile. Different from the conventional 3D transformation-optical pencil-beam lenses, we carry out the 3D modeling through revolving the prescribed transformational media 180 degrees
Wang et al. ... Lei and Liu suggest that actors tend to form densely-knit groups according to particular relationships [29, 30] . ...doi:10.1007/s10115-012-0555-0 fatcat:kufnddbugrbzfkfgta2ngiqzcq
Cytotoxicity of PEG oligomers (with different molecular weights) and PEG based monomers (with different chain end groups) was studied in detail.doi:10.1039/c7ra00861a fatcat:nooqn3yr7jfafij5rbyajpxjjy
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