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Macrophage response to methacrylate conversion using a gradient approach

Nancy J. Lin, LeeAnn O. Bailey, Matthew L. Becker, Newell R. Washburn, Lori A. Henderson
2007 Acta Biomaterialia  
Incomplete conversion, an ongoing challenge facing photopolymerized methacrylate-based polymers, affects leachables as well as the resulting polymer network. As novel polymers and composites are developed, methods to efficiently screen cell response to these materials and their properties, including conversion, are needed. In this study, an in vitro screening methodology was developed to assess cells cultured directly on cross-linked polymer networks. A gradient in methacrylate double bond
more » ... rsion was used to increase the experimental throughput. A substrate of 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl] propane (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) was prepared with a conversion ranging from 43.0% to 61.2%. Substrates aged for 7 days had no significant differences in surface roughness or hydrophilicity as a function of conversion. Leachables were detectable for at least 7 days using UV absorption, but their global cytotoxicity was insignificant after 5 days of aging. Thus, RAW 264.7 macrophage-like cells were cultured on aged substrates to evaluate the cell response to conversion, with possible contributions from the polymer network and local leachables. Conversions of 45% and 50% decreased viability (via calcein/ethidium staining) and increased apoptosis (via annexin-V staining). No significant changes (p > 0.05) in tumor necrosis factor-a and interleukin-1b gene expression, as measured by quantitative, real-time reverse transcription-polymerase chain reaction, were seen as conversion increased. Thus, conversions greater than 50% are recommended for equimolar BisGMA/TEGDMA. The ability to distinguish cell response as a function of conversion is useful as an initial biological screening platform to optimize dental polymers.
doi:10.1016/j.actbio.2006.10.001 pmid:17140868 fatcat:enmv7srosvdjtnjyrhgsgdog4m

Development of a model for the representation of nanotechnology-specific terminology

LeeAnn O Bailey, Christopher H Kennedy, Martin J Fritts, Francis W Hartel
2006 AMIA Annual Symposium Proceedings  
Nanotechnology is an important, rapidly-evolving, multidisciplinary field. The tremendous growth in this area necessitates the establishment of a common, open-source terminology to support the diverse biomedical applications of nanotechnology. Currently, the consensus process to define and categorize conceptual entities pertaining to nanotechnology is in a rudimentary stage. We have constructed a nanotechnology-specific conceptual hierarchy that can be utilized by end users to retrieve
more » ... controlled terminology regarding emerging nanotechnology and corresponding clinical applications.
pmid:17238469 pmcid:PMC1839578 fatcat:2rrsmsmdz5eaxk5hvkp4f26nsi

Encapsulated chondrocyte response in a pulsatile flow bioreactor

James A. Cooper, Wan-Ju Li, LeeAnn O. Bailey, Steve D. Hudson, Sheng Lin-Gibson, Kristi S. Anseth, Rocky S. Tuan, Newell R. Washburn
2007 Acta Biomaterialia  
We have developed a bioreactor-based millifluidic technique that allows for dynamic culture conditions and measurement of the fluid flow impinging upon a three-dimensional tissue engineering scaffold. Chondrocytes in scaffolds have been shown to require mechanical stimulation to produce an extracellular matrix that resembles native cartilage. This study investigates the effect of pulsatile flow on chondrocyte response in a model poly(ethylene glycol) dimethacrylate hydrogel. Bovine chondrocytes
more » ... were encapsulated in the hydrogel and cultured for 7, 14 and 21 days at pulsatile flow frequencies of 0.5 Hz (15 ml/min) and 1.5 Hz (17 ml/min). The scaffolds cultured under dynamic conditions were compared to those cultured under static (non-flow) conditions. Quantitative real-time reverse transcription polymerase chain reaction was used to quantify collagen type I, collagen type II and aggrecan gene copy numbers as markers for chondrocyte phenotypic expression. Histological sections stained with hematoxylin & eosin, and Alcian blue confirmed chondrocyte morphology and matrix formation. Interestingly, regulation of the collagen type II gene was particularly sensitive to the flow conditions. The understanding of the cell response to encapsulation and flow could be used to identify the appropriate culture conditions necessary to design and develop hydrogel carriers to promote the formation of extracellular matrix as well as to further our knowledge of chondrocyte mechanobiology.
doi:10.1016/j.actbio.2006.08.010 pmid:17097360 fatcat:gkgoplcswzfkfbq4fg4yfeobeu

Quantification of inflammatory cellular responses using real-time polymerase chain reaction

LeeAnn O. Bailey, Newell R. Washburn, Carl G. Simon, Edward S. Chan, Francis W. Wang
2004 Journal of Biomedical Materials Research  
Bailey; e-mail: leeann.bailey@nist.gov Contract grant sponsor: NIST/NIDCR; contract grant number: Y1-DE-1021 (B) demonstrates the amplification plot of the RAW 264.7 samples only.  ... 
doi:10.1002/jbm.a.20134 pmid:15058003 fatcat:dcewkydfpreexfeyaqza4vynym

The Continuing Umbrella of Research Experiences (CURE): A Model for Training Underserved Scientists in Cancer Research

Idalid Franco, LeeAnn O. Bailey, Alexis D. Bakos, Sanya A. Springfield
2010 Journal of Cancer Education  
Mentoring is a critical aspect of research and training; and the adoption of a successful mentoring model for guiding researchers through the educational pipeline is lacking. The Continuing Umbrella of Research Experiences (CURE) program was established in the Comprehensive Minority Biomedical Branch; which is part of the National Cancer Institute. This program offers unique training and career development opportunities to enhance diversity in cancer research. The CURE initiative focuses on
more » ... dening the cadre of underserved investigators engaging in cancer research. CURE begins with high school students and fosters scientific, academic and research excellence throughout the trainee's educational progression. The program supports students throughout the entirety of their training career. During this period, the trainee matures into a competitive early stage investigator; capable of securing advanced research project funding in academic and industry workforces. Thus, the CURE program provides a comprehensive vehicle for training and reinforces the critical mass of underserved investigators conducting cancer research.
doi:10.1007/s13187-010-0126-6 pmid:20568030 pmcid:PMC3008508 fatcat:aakvf33pxzcrjjxps4btvelili

Cellular response to phase-separated blends of tyrosine-derived polycarbonates

LeeAnn O. Bailey, Matthew L. Becker, Jean S. Stephens, Nathan D. Gallant, Christine M. Mahoney, Newell R. Washburn, Aarti Rege, Joachim Kohn, Eric J. Amis
2006 Journal of Biomedical Materials Research. Part A  
polycarbonates were synthesized as described previously. 9 In these studies, the alkyl ester pendent chains (R) of the poly(desaminotyrosyl tyrosine ester carbonate) are either an ethyl (E) or octyl (O)  ... 
doi:10.1002/jbm.a.30527 pmid:16278865 pmcid:PMC2996268 fatcat:mvyj6b3izncj7iehrbvess6gti

Screen to Save: Results from NCI's Colorectal Cancer Outreach and Screening Initiative to Promote Awareness and Knowledge of Colorectal Cancer in Racial/Ethnic and Rural Populations

Damiya E. Whitaker, Frederick R. Snyder, Sandra L. San Miguel-Majors, LeeAnn O. Bailey, Sanya A. Springfield
2020 Cancer Epidemiology, Biomarkers and Prevention  
The Center to Reduce Cancer Health Disparities (CRCHD), NCI, implemented Screen to Save, NCI's Colorectal Cancer Outreach and Screening Initiative to promote awareness and knowledge of colorectal cancer in racial/ethnic and rural populations. The initiative was implemented through CRCHD's National Outreach Network (NON). NON is a national network of Community Health Educators (CHE), aligned with NCI-designated Cancer Centers across the nation. In phases I and II, the CHEs focused on the
more » ... ation of cancer-related information and implementation of evidence-based educational outreach. In total, 3,183 pre/post surveys were obtained from male and female participants, ages 50 to 74 years, during the 347 educational events held in phase I. Results demonstrated all racial/ethnic groups had an increase in colorectal cancer-related knowledge, and each group strongly agreed that the educational event increased the likelihood that they would engage in colorectal cancer-related healthful behaviors (e.g., obtain colorectal cancer screening and increase physical activity). For phase II, Connections to Care, event participants were linked to screening. Eighty-two percent of the participants who obtained colorectal cancer screening during the 3-month follow-up period obtained their screening results. These results suggest that culturally tailored, standardized educational messaging and data collection tools are key change agents that can serve to inform the effectiveness of educational outreach to advance awareness and knowledge of colorectal cancer. Future initiatives should focus on large-scale national efforts to elucidate effective models of connections to care, related to colorectal cancer screening, follow-up, and treatments that are modifiable to meet community needs.
doi:10.1158/1055-9965.epi-19-0972 pmid:32358038 fatcat:aibtqnnoynaddcjuedowwu73qa

Page 1009 of Psychological Abstracts Vol. 82, Issue 2 [page]

1995 Psychological Abstracts  
Bailey, Paul, 5567 Bailey, Richard & 7345 Bailey, S.  ...  Chris, 5918 Bagley, Christopher, 5741 ito, Stephen J., 4117 Bahro, Marcel, 6769 Bailey, Elaine K., "7510 Bailey, J., 7194 Bailey, Jon S., 6642 Bailey, P.  ... 

Page 2511 of Psychological Abstracts Vol. 89, Issue 6 [page]

2002 Psychological Abstracts  
, Di, 18822 Bailey, Glynis K., 16465 Bailey, Karl G.  ...  D., 16341 Bailey, Nicola M., 18691 Bailey-Rogers, Sarah, 16157 Baker, Bruce L., 18711 Baker, David W. , 16185 Baker, Forest, 16419 Baker, Tom, 19308 Bakken, Jeffrey P., 19101 Bakker, Frank C. , 16359 Balaban  ... 

Franklin R. Buchanan: (October 18, 1914–October 11, 2003)

Lucien Ellington
2004 Journal of Asian Studies  
He will be missed, especially by his wife, Emily, and his daughters, Kwan-Yin and LeeAnn.  ...  He is one of the three pioneers (along with Jackson Bailey and Elgin Heinz) who created the movement to improve teaching about Asia in the nation's schools.  ... 
doi:10.1017/s0021911804000932 fatcat:unpeagtucbehtprxfpwfqtkpty

CON volume 32 issue 3 Cover and Back matter

2017 Continuity and Change  
email: mnagata@fmarion.edu Dr Julie Marfany, Department of History, University of Durham, 43 North Bailey, Durham DH1 3EX, UK.  ...  H E E B Ø L L H O L M , Law, order and plunder at sea: a comparison of England and France in the fourteenth century 37 J U S T Y N A W U B S M R O Z E W I C Z , The late medieval and early modern Hanse  ... 
doi:10.1017/s0268416017000388 fatcat:ehwfjhia4fej5bpnqg3ixyxf5y

Page 835 of The Athenaeum Vol. , Issue 3687 [page]

1898 The Athenaeum  
If Leeann obtain it of your tradesman, a tin will be sent post free for ne sta ape JAMES EPPS & CO., Limited, Homeopathic Chemists, London.  ...  Meetkerke. o SHAKESPEARE'S EARL OF PEMBROKE. By Sylvanus Urban. London: CHATTO & WINDUS, 111, St. Martin’s Lane, W.C.  ... 

Page 20 of None Vol. 27, Issue 76 [page]

1977 None  
I you are and want to go and possibh have some means of transportation, o are into forming a club, let me know Sue 831-2560. I Ship your stuff home with us— I I TO NYC. YONKERS.  ...  LEEANNE, want to get- together? Dan from Calculus 636-4294. GOOD RATES, good work, good people. Need your car fixed? Call Rock Automotive for excellent service. All work guaranteed. 695-3607.  ... 

Page 224 of Journal of Education Vol. 11, Issue 264 [page]

1880 Journal of Education  
BAILEY, Agt., 23 Franklin St., Boston. os os B 4s Psychology, English Literature, etc. Full list, with specimen pages, mailed on application . GEO. B.  ...  Pk pre- ‘mite et +e I lem, of Static as nd lS me Typography, Pr Puste oo Leeann are in the) Speller, e « ° © 10 15 ae Gradation of exerciss at ance af ror introductory Prices Greatly Reduced. oe ¥ VAN  ... 

Stability of SARS-CoV-2 IgG in multiple laboratory conditions and blood sample types

Jamil N Kanji, Ashley Bailey, Jayne Fenton, L. Robbin Lindsay, Antonia Dibernardo, Nikki PL Toledo, Brooks Waitt, Nadine Lecoq, Carla Osiowy, Elizabeth Giles, Jacqueline Day, William Stokes (+3 others)
2021 Journal of Clinical Virology  
at -70 o C, -20 o C, 4 o C, or room temperature; and two quantitative assays at -20 o C.  ...  Page 5 of 17 patient were stored at three different temperatures (-70 o C, 4 o C, and room temperature (21-25 o C)) and thawed only on the day of testing to assess the stability of SARS-CoV-2 IgG.  ... 
doi:10.1016/j.jcv.2021.104933 pmid:34364133 pmcid:PMC8310571 fatcat:qegrqxjjwzbfteihamen7lq6ra
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