A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
Taken together our data suggest that MK-STYX may be OPEN ACCESS Citation: Flowers BM, Rusnak LE, Wong KE, Banks DA, Munyikwa MR, et al. (2014) The Pseudophosphatase MK-STYX Induces Neurite-Like Outgrowths ...doi:10.1371/journal.pone.0114535 pmid:25479605 pmcid:PMC4257672 fatcat:5nsp6avpbjd6fbuauncxddsqh4
In this manuscript, Zenggang and colleagues have developed protein-protein interaction networks in lung cancer cells to identify many new interactions. They have also reported new regulatory mechanisms for the genes such as MYC, STK11 and CDK4 in lung canc er. This manuscript also aims to associate tumor suppressors to drug targets and also explores therapeutic options. The presented dataset of protein-protein interactions in lung cancer cells would be useful for detailed mechanisticdoi:10.1038/ncomms14356 pmid:28205554 pmcid:PMC5316855 fatcat:654xnndk7jhzdnvmxm3eqdfe7m
more »... ng of lung ca ncer related genes. The high-throughput approach followed in the manuscript to identify PPI networks is impressive, but the conclusions drawn are not well supported from the reported experiments and need more evidence to strengthen them. In addition, man y key methodological details are lacking, which make the manuscript hard to follow at many places in its current form. Some additional analyses would also improve the significance of this study. Specific comments that need to be addressed are listed below: Major comments: 1. The high-throughput identification of PPI networks have historically had a high false positive rate. How false positive connections were identified and filtered out from the FRET experiments is not clear. The authors could consider a set of random pairs of genes, which are known to be non-binders as a "negative control" in their FRET experiments? Figure 2A shows very high connectivity of the genes altered in lung cancer, which doesn't seem plausible. It is not clear how each bait protein is interacting with almost every other protein considered in this study (Table S2) . Is it because of the bias in the scoring scheme? Why FOC values were not normalized for signals of any two non-binders? In particular the choice of negative control is crucial for HT experiments such as these as they can be biased by promiscuity in interactors, and low off-rate of FRET signal. In addition, as a control the authors should show that FRET signal is independent of the single Venus or GST signal. 2. Authors have performed three independent experiments in triplicates for both fusions for 83 lung cancer genes in pairwise manner. However, they haven't discussed the reproducibility of interactions in different replicates. What is the specificity of the identified interactions? How correlated are signals when the Venus-GST pairs are swapped (so called A-B, B-A correlation)? These variables will help in identifying the high confidence interactors from such a huge datasets. 3. It is not clear if the overlap of the identified PPI dataset with other available datasets is significant. An enrichment analysis using known interactions can show the significance of the overlap. A sufficiently detailed venn diagram representation of overlap with all the known datasets (eg. STRING, Bioplex) can better explain how many known interactions were
Rusnak, Alain Philippe Salas, David A. Sandefur, Windie Christine Sims-McKay, John Travis Smiley, Jeannine Gale Mehlberg- Smith, TTiomas Patrick Smith, III, Sean Karl Stevens, Cherie D. ... Baker, Eugene Everett Bledsoe, Kent Richard Bohac, Michael George Carbone, Ralph Steven Cardin, Curtis Erikson Cook, Lauren Rachel Davis, Rhonda S. Felheim, Scott M. Ferguson, Steven Finck, Kassie R. ...
Rose, Lauren L. .......... RNIN. besueiscsssncvees eben cae vane 65074 Rosen, Michael I. ............... See * 11002 Rosenberg, I. G. .......... III, NS... ... Rusnak, J. Russo, E. RO FONGE GS, 506. 600 <ctcccccs Rustamov, Kh. P. Ruszczynski, Andrzej Ny 5 ene ee ema, eT 47015 PT Ne oc cinacesecevsccssvenere 03006 Rybicki, Slawomir Ryckkov, S. ...
J., 3148 Rush, Stephen, 4505 Rusnak, Milan, 4591 Russ, Frederick A., 6065 Russell, B. ... ., 4976 Ratcliffe, Laurene, 3579 Ratinckx, Elie, 3964 Ratsepp, Marju, 5138 Rau, Devaki, 4366 Rauch, Jodee, 6001 Raudenbush, Stephen L., 4364 Raudenbush, Stephen W., 3235 Ravaglia, Giovanni, 5049 Ravindran ...
Medical writing support was provided by Lauren Rusnak, data (provided animals, acquired and managed patients, provided facilities, etc.): 349 Y.-K. Kang, Y.-J. Bang, S. Kondo, H.C. Chung, K. Muro, T. ... Tumor histology was initially captured using the Japanese or World Health Organization 134 classification systems and later mapped to the Lauren classification system (Supplementary 128 133 135 ...doi:10.1158/1078-0432.ccr-19-3806 pmid:32299818 fatcat:tnqrekboivhadm2al4pp6jrlm4
Medical writing support was provided by Lauren Rusnak, PhD, of ClinicalThinking, Inc, and funded by Merck KGaA and GlaxoSmithKline in accordance with Good Publication Practice (GPP3) guidelines (http:/ ... Tumor histology was initially captured using the Japanese or World Health Organization classification systems and later mapped to the Lauren classification system (Supplementary Table S1 ; refs.  ... with heavily pretreated advanced GC/GEJC (61% received ≥3 prior lines of Histology was initially captured using the Japanese or World Health Organization classification systems and later mapped to the Lauren ...doi:10.1158/1078-0432.ccr-19-3806 fatcat:qytibseqgfakrmwl6e67bi633y
British Journal of Psychotherapy, 1996(Fal), Vol 13(1), 37-52. 30624 Russell, Connie L.; Bard, Kim A. & Adamson, Lauren B. Social referencing by young chimpanzees (Pan troglodytes). ... American Journal of Geriatric Psychiatry, 1996(Sum), Vol 4(3), 208-217. 19165 Rusnak, Kristin— See Carlson, John G. 1737 Russ, Mark J.; Clark, W. Crawford; Cross, Lisa W.; Kemper- man, Ingrid et al. ...
We thank Lauren Rusnak for editing the manuscript. ...doi:10.1038/onc.2017.180 pmid:28628118 pmcid:PMC5648601 fatcat:yuydmfcgazgz7kddauiyozozpu
Medical writing support was provided by Lauren Rusnak, PhD, of ClinicalThinking Inc., Hamilton, NJ, USA, which was also funded by Merck KGaA and GlaxoSmithKline in accordance with Good Publication Practice ...doi:10.1007/s11523-021-00810-9 pmid:33840050 pmcid:PMC8266718 fatcat:bz4aqgkdlbbvbi24i7vst3utm4
Additionally, video chat may offer opportunities for young children to explore and experiment with how screen media relate to their in-person world (Myers et al., 2017; Rusnak & Barr, 2020; Strouse et ... young children to eventually build a nascent concept of screen-based digital technology: how events on a screen relate to (but are not the same as) events in the real world (Myers, Keyser, & Cors, 2019; Rusnak ...doi:10.1002/hbe2.268 fatcat:64qqt7zrjngxxkojxo4csb3cge
Genotyping and mouse colony maintenance were done by Tatiana Iakovleva, Gretchen Ireland, Lauren Rusnak, and Brenda Lager. ...doi:10.1128/mcb.01456-06 pmid:17030606 pmcid:PMC1800641 fatcat:noqyhcmd2vgzjbetxcwkuyqcpa
Wengenack NL, Uhl JR, St Amand AL, Tomlinson AJ, Benson LM, Naylor S, Kline BC, Cockerill FR, 557 3rd, Rusnak F. 1997. ...doi:10.1101/322750 fatcat:xuy6iixvazeofnegaosqzto4ri
., Rusnak, D. W., Owens, G., Alligood, K. J., and Spector, N. ... Derived from MD Simulations of Candidate Compounds Predicted to Coordinate the Bridging Water HIVPR ■ ACKNOWLEDGMENTS ACKNOWLEDGMENTS The authors thank Dwight McGee, Yuchen Zhou, Courtney Singleton, Lauren ...doi:10.1021/acs.biochem.8b00524 pmid:29975516 pmcid:PMC6110523 fatcat:oy6o4tlfd5ao5ceqcoevpez624
Second row L to R: Mi- chele Raya, Maria Rusnak, Chiarme Paras, Rosemary Boatright (Secre tary), Sharon Cook, Monique Van- Son (Treasurer), Terri Melvin. ... Front row L to R: Lauren Ledbetter, Bonnie Kieta, Pablo Mendoza, Roxanne Short, Cindy Beezley, Michele Raya. ...fatcat:25v4k7t5g5esxbjk3u3pkse3jq
« Previous Showing results 1 — 15 out of 26 results