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Plant Communities Suitable for Green Roofs in Arid Regions

Rachel Gioannini, Malik Al-Ajlouni, Richard Kile, Dawn VanLeeuwen, Rolston St. Hilaire
2018 Sustainability  
In extensive green roof settings, plant communities can be more robust than monocultures.  ...  In addition, native plants might be hardier and more ecologically sound choices than non-native plants in green roof systems.  ...  To date, few green roofs exist in the desert southwest region of the US, and few studies have explored the use of green roof plants for this zone.  ... 
doi:10.3390/su10061755 fatcat:3jn24dxj5ndgngbem2aijqwlqi

Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Dendritic Cell Maturation and Function

Monica Reis, Emily Mavin, Lindsay Nicholson, Kile Green, Anne M. Dickinson, Xiao-nong Wang
2018 Frontiers in Immunology  
We found a predominant association between MSC-EVs and DCs detected by the fluorescence staining overlap of PKH26 labeled MSC-EVs (red) and HLA-DR labeled DCs (green).  ...  Representative images from confocal microscopy showing that EV-PKH26 + labeled MSC-EVs (red) are closely associated with DCs (green). No uptake of MSC-EVs by CD3 + T cells (blue) was detected.  ... 
doi:10.3389/fimmu.2018.02538 pmid:30473695 pmcid:PMC6237916 fatcat:qkmuc3j4avasnpowqhpni4uul4

Early Molecular Stratification of High-risk Primary Biliary Cholangitis

Claire Hardie, Kile Green, Laura Jopson, Ben Millar, Barbara Innes, Sarah Pagan, Dina Tiniakos, Jessica Dyson, Muzlifah Haniffa, Venetia Bigley, David E Jones, John Brain (+1 others)
2016 EBioMedicine  
Pathological Scoring The scoring of pathological specimens was simultaneously performed by two pathologists (JGB and DGT) using H&E, Orcein and Sirius red fast green histological stains.  ... 
doi:10.1016/j.ebiom.2016.11.021 pmid:27913155 pmcid:PMC5161439 fatcat:3rgb5aucafavfgtdwuli7je7cu

Expression of Serum microRNAs is Altered During Acute Graft-versus-Host Disease

Rachel E. Crossland, Jean Norden, Mateja Kralj Juric, Kile Green, Kim F. Pearce, Clare Lendrem, Hildegard T. Greinix, Anne M. Dickinson
2017 Frontiers in Immunology  
Xiao et al. based their assessment on Sybr-green dye, which lacks specificity in comparison to the TaqMan low-density array employed by Gimondi et al. (17) .  ... 
doi:10.3389/fimmu.2017.00308 pmid:28392786 pmcid:PMC5364146 fatcat:43x6eh64ozhddpzvgvnjm35uvy

Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace

Laura Jardine, Sarah Wiscombe, Gary Reynolds, David McDonald, Andrew Fuller, Kile Green, Andrew Filby, Ian Forrest, Marie-Helene Ruchaud-Sparagano, Jonathan Scott, Matthew Collin, Muzlifah Haniffa (+1 others)
2019 Nature Communications  
square), intermediate (divided green square) and non-classical (open green square) monocytes.  ...  blood and arise independently of monocyte- Fig. 3 CD1c + DCs in LPS-BAL are likely to be tissue-recruited blood CD1c + DCs. a Expression of surface antigens by flow cytometry on DC2/3 from HC blood (green  ... 
doi:10.1038/s41467-019-09913-4 pmid:31040289 pmcid:PMC6491485 fatcat:hvewjfqm2jevfp3v7hcz5ewjp4

Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis

Arianna Bianchi, Letizia Marchetti, Zoe Hall, Henrique Lemos, Michele Vacca, Hannah Paish, Kile Green, Bronte Elliott, Dina Tiniakos, João F. Passos, Diana Jurk, Derek A. Mann (+1 others)
2021 Journal of Immunology  
Right graph:The sedentary group (green line) shows a higher n6/n3 ratio correlates with a higher number of B220 + , B cells.  ...  (G) SPSS correlations: Left graph: In the sedentary group (green line), a higher number of CD3 cells positively correlates with a higher n6/n3 ratio of PCs.  ... 
doi:10.4049/jimmunol.2001022 pmid:33441438 pmcid:PMC7851741 fatcat:ck74cttwlnearn6eemyvcevgki

Care pathway and prioritization of rapid testing for COVID-19 in UK hospitals: a qualitative evaluation

Timothy Hicks, Amanda Winter, Kile Green, Patrick Kierkegaard, D Ashley Price, Richard Body, A Joy Allen, Sara Graziadio, CONDOR Steering Group
2021 BMC Health Services Research  
Following the results, the patients were cohorted accordingly into Red, Pink, Yellow, Green, or Hyper Green zones. Zones donated as a different colour, i.e.  ...  In general, these areas can be split into 3 categories: Green, Yellow, and Red.  ... 
doi:10.1186/s12913-021-06460-x pmid:34059036 fatcat:kh54bshhc5hkngvpqgcxdxajbi

Donor monocyte-derived macrophages promote human acute graft versus host disease [article]

Laura Jardine, Urszula Cytlak, Merry Gunawan, Gary Reynolds, Kile Green, Xiao-Nong Wang, Sarah Pagan, Maharani Pradnya, Christopher A Lamb, Anna K Long, Erin Hurst, Smeera Nair (+6 others)
2019 biorxiv/medrxiv   pre-print
(B) Whole-mount immunofluorescence of dermis from healthy controls and patients with GVHD, as indicated with antibodies to CD3 (red), CD11c, (green), and FXIIIA (blue). Scale bar: 50 μm.  ... 
doi:10.1101/787036 fatcat:ivqqmyxmgfdxvb3dywmp4mubjy

Utility of Routine Laboratory Biomarkers to Detect COVID-19: A Systematic Review and Meta-Analysis

Jana Suklan, James Cheaveau, Sarah Hill, Samuel G Urwin, Kile Green, Amanda Winter, Timothy Hicks, Anna E Boath, Ashleigh Kernohan, D Ashley Price, A Joy Allen, Eoin Moloney (+1 others)
2021 Viruses  
No routine laboratory biomarkers perform well enough in diagnosing COVID-19 in isolation for them to be used as a standalone diagnostic test or to help clinicians prioritize patients for treatment. Instead, other diagnostic tests are needed. The aim of this work was to statistically summarise routine laboratory biomarker measurements in COVID-19-positive and -negative patients to inform future work. A systematic literature review and meta-analysis were performed. The search included names of
more » ... monly used, routine laboratory tests in the UK NHS, and focused on research papers reporting laboratory results of patients diagnosed with COVID-19. A random effects meta-analysis of the standardized mean difference between COVID-19-positive and -negative groups was conducted for each biomarker. When comparing reported laboratory biomarker results, we identified decreased white blood cell, neutrophil, lymphocyte, eosinophil, and platelet counts; while lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase were elevated in COVID-19-positive compared to COVID-19-negative patients. Differences were identified across a number of routine laboratory biomarkers between COVID-19-positive and -negative patients. Further research is required to identify whether routine laboratory biomarkers can be used in the development of a clinical scoring system to aid with triage of patients.
doi:10.3390/v13050803 pmid:33946171 fatcat:yorrxetpbzgazmrh7wv7d7wbzy

Epithelial damage and tissue γδ T cells promote a unique tumor-protective IgE response

Greg Crawford, Mark David Hayes, Rocio Castro Seoane, Sophie Ward, Tim Dalessandri, Chester Lai, Eugene Healy, David Kipling, Charlotte Proby, Colin Moyes, Kile Green, Katie Best (+4 others)
2018 Nature Immunology  
Real-time PCR product was detected with SYBR Green (Life) measured continuously with a ViiA 7 Real- Immunoglobulin sequencing and analysis Mice were treated twice with DMBA or TPA to the dorsal side  ... 
doi:10.1038/s41590-018-0161-8 pmid:30013146 pmcid:PMC6071860 fatcat:4mwvaqhb2nfa5ig73cie3a6tny

Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans

Urszula Cytlak, Anastasia Resteu, Sarah Pagan, Kile Green, Paul Milne, Sheetal Maisuria, David McDonald, Gillian Hulme, Andrew Filby, Benjamin Carpenter, Rachel Queen, Sophie Hambleton (+6 others)
2020 Immunity  
The formation of mammalian dendritic cells (DCs) is controlled by multiple hematopoietic transcription factors, including IRF8. Loss of IRF8 exerts a differential effect on DC subsets, including plasmacytoid DCs (pDCs) and the classical DC lineages cDC1 and cDC2. In humans, cDC2-related subsets have been described including AXL+SIGLEC6+ pre-DC, DC2 and DC3. The origin of this heterogeneity is unknown. Using high-dimensional analysis, in vitro differentiation, and an allelic series of human IRF8
more » ... deficiency, we demonstrated that cDC2 (CD1c+DC) heterogeneity originates from two distinct pathways of development. The lymphoid-primed IRF8hi pathway, marked by CD123 and BTLA, carried pDC, cDC1, and DC2 trajectories, while the common myeloid IRF8lo pathway, expressing SIRPA, formed DC3s and monocytes. We traced distinct trajectories through the granulocyte-macrophage progenitor (GMP) compartment showing that AXL+SIGLEC6+ pre-DCs mapped exclusively to the DC2 pathway. In keeping with their lower requirement for IRF8, DC3s expand to replace DC2s in human partial IRF8 deficiency.
doi:10.1016/j.immuni.2020.07.003 pmid:32735845 pmcid:PMC7447982 fatcat:vzz2mvd625dtdpntsb25hs2aqa

FebriDx point-of-care test in patients with suspected COVID-19: a pooled diagnostic accuracy study [article]

Samuel G Urwin, B Clare Lendrem, Jana Suklan, Kile Green, Sara Graziadio, Peter Buckle, Paul M Dark, Adam L Gordon, Daniel S Lasserson, Brian Nicholson, D Ashley Price, Charles Reynard (+7 others)
2020 medRxiv   pre-print
Point-of-care (POC) tests for COVID-19 could relieve pressure on isolation resource, support infection prevention and control, and help commence more timely and appropriate treatment. We aimed to undertake a systematic review and pooled diagnostic test accuracy study of available individual patient data (IPD) to evaluate the diagnostic accuracy of a commercial POC test (FebriDx) in patients with suspected COVID-19. Methods: A literature search was performed on the 1st of October 2020 to
more » ... studies reporting diagnostic accuracy statistics of the FebriDx POC test versus real time reverse transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2. Studies were screened for risk of bias. IPD were sought from studies meeting the inclusion and exclusion criteria. Logistic regression was performed to investigate the study effect on the outcome of the RT-PCR test result in order to determine whether it was appropriate to pool results. Diagnostic accuracy statistics were calculated with 95% confidence intervals (CIs). Results: 15 studies were screened, and we included two published studies with 527 hospitalised patients. 523 patients had valid FebriDx results for Myxovirus resistance protein A (MxA), an antiviral host response protein. The FebriDx test produced a pooled sensitivity of 0.920 (95% CI: 0.875-0.950) and specificity of 0.862 (0.819-0.896) compared with RT-PCR, where there was an estimated true COVID-19 prevalence of 0.405 (0.364-0.448) and overall FebriDx test yield was 99.2%. Patients were tested at a median of 4 days [interquartile range: 2:9] after symptom onset. No differences were found in a sub-group analysis of time tested since the onset of symptoms. Conclusions: Based on a large sample of patients from two studies during the first wave of the SARS-CoV-2 pandemic, the FebriDx POC test had reasonable diagnostic accuracy in a hospital setting with high COVID-19 prevalence, out of influenza season. More research is required to determine how FebriDx would perform in other healthcare settings with higher or lower COVID-19 prevalence, different patient populations, or when other respiratory infections are in circulation.
doi:10.1101/2020.10.15.20213108 fatcat:24dpgklru5fydpr6y2eqsmyxsm

Impact of Alemtuzumab Scheduling on Graft-versus-Host Disease after Unrelated Donor Fludarabine and Melphalan Allografts

Kile Green, Kim Pearce, Rob S. Sellar, Laura Jardine, Phillip L.R. Nicolson, Sandeep Nagra, Venetia Bigley, Graham Jackson, Anne M. Dickinson, Kirsty Thomson, Stephen Mackinnon, Charles Craddock (+2 others)
2017 Biology of Blood and Marrow Transplantation  
Authorship contributions Kile Green analyzed data and produced figures Kim Pearce performed statistical analysis Rob S Sellar provided clinical data Laura Jardine analyzed data and produced figures Phillip  ... 
doi:10.1016/j.bbmt.2017.02.007 pmid:28212937 fatcat:kbbgsewjbfdllncuthyl5l27sm

How to Ease the Pain of Taking a Diagnostic Point of Care Test to the Market: A Framework for Evidence Development

Sara Graziadio, Amanda Winter, B. Clare Lendrem, Jana Suklan, William S. Jones, Samuel G. Urwin, Rachel A. O'Leary, Rachel Dickinson, Anna Halstead, Kasia Kurowska, Kile Green, Andrew Sims (+3 others)
2020 Micromachines  
Products below the diagonal green line (D1) may provide an opportunity for savings, while those above the line may not be good value.  ...  The green diagonal line shows the threshold above which the product would be considered poor value (B1), and below which it would be considered good value (B2).  ... 
doi:10.3390/mi11030291 pmid:32164393 pmcid:PMC7142698 fatcat:ohj35m3aorbhjd2tj24g3n5eba

Human Dermal CD14 + Cells Are a Transient Population of Monocyte-Derived Macrophages

Naomi McGovern, Andreas Schlitzer, Merry Gunawan, Laura Jardine, Amanda Shin, Elizabeth Poyner, Kile Green, Rachel Dickinson, Xiao-nong Wang, Donovan Low, Katie Best, Samuel Covins (+13 others)
2014 Immunity  
(D) Pseudocolor images of whole-mount skin immunostained for LYVE-1 (green), CD209 (DCSIGN) (red), and FXIIIa (blue).  ...  Lower panel; T48 depicts HLA-DR + (green) cells mainly located within lymphatic vessels but DCSIGN + cells are outside the lymphatic vessels.  ... 
doi:10.1016/j.immuni.2014.08.006 pmid:25200712 pmcid:PMC4175180 fatcat:v5x4pjwkpffr3i4hypff5c4rvq
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