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AbstractWe report a new computed tomography reconstruction method, named quantisation units reconstruction technique (QURT), applicable to electron and other fields of tomography. Conventional electron tomography methods such as filtered back projection, weighted back projection, simultaneous iterative reconstructed technique, etc. suffer from the 'missing wedge' problem due to the limited tilt-angle range. QURT demonstrates improvements to solve this problem by recovering a structural imagedoi:10.1038/s41598-020-77156-1 pmid:33214577 pmcid:PMC7678869 fatcat:5cy5xsbr55h2zbhbw7jx4fzwqi
more »... rred due to the missing wedge and substantially reconstructs the structure even if the number of projection images is small. QURT reconstructs a cross-section image by arranging grey-level quantisation units (QU pieces) in three-dimensional image space via unique discrete processing. Its viability is confirmed by model simulations and experimental results. An important difference from recently developed methods such as discrete algebraic reconstruction technique (DART), total variation regularisation—DART, and compressed sensing is that prior knowledge of the conditions regarding the specimen or the expected cross-section image is not necessary.
Prekallikrein (PK) deficiency is an uncommon disorder in dogs. In this report, we describe a case of a dog that was referred for neurological defects and had a prolonged activated partial thromboplastin time (aPTT) and normal prothrombin time (PT) with no hemostatic defects. By using human PK-deficient plasma, the dog was diagnosed to have PK deficiency. The nucleotide sequence of normal canine PK cDNA was determined and compared with the genomic sequences of PK in the affected dog. Thedoi:10.1292/jvms.10-0207 pmid:20736516 fatcat:hzyyla6ufzh6phooobo7o4hywa
more »... on revealed that the dog had a point mutation in exon 8 that leads to an amino acid substitution in the fourth apple domain of PK. This is the first report showing a point mutation of PK in a dog with PK deficiency. KEY WORDS: canine, mutation, prekallikrein deficiency.
The Japanese Journal of Antibiotics
Baba K et al. ...doi:10.2332/allergolint.54.203 fatcat:dvztvu7f2vhinod7k2l2zflcdq
Objectives: To investigate the correlation between electroneurography (ENoG) findings and the prognosis of Bell's palsy in children compared with adults. Methods: Twenty-two children and 92 adults with Bell's palsy who underwent ENoG between 8 days and 4 weeks from the onset of symptoms were retrospectively enrolled. The time to maximal recovery and rate of favorable recovery (House-Brackmann grade I or II) was assessed. Children (C) and adults (A) were further subdivided into low (G10%) ordoi:10.1097/mao.0b013e31823556ae pmid:21997587 fatcat:fnx4vymgubd2hero3uapfrcb3u
more »... (R10%) subgroups according to their ENoG values (affected versus unaffected side) at initial evaluation. The numbers in each subgroup were as follows: C-low (n = 8), A-low (n = 21), C-high (n = 14), and A-high (n = 71). Results: Of the 22 children assessed, 2 of the 4 patients who showed a total loss of evoked potentials on the affected side (0% ENoG value) exhibited an unfavorable recovery. The remaining 20 patients achieved a favorable recovery eventually. Patients in group C-low reached a maximal recovery of facial movement significantly later than those in group C-high ( p G 0.001). Time to maximal recovery of facial movement in group A-low was later than that in group C-low, although the difference was not statistically significant ( p = 0.15). The patients in group A-high reached a maximal recovery significantly later than those in group C-high ( p G 0.05). Conclusion: Bell's palsy seems to recover earlier in children than adults when matched for severity. The presence of an identifiable response in ENoG, irrespective of its amplitude, may indicate a favorable recovery of facial movement in children.
The aim of the present study was to investigate the aberrant methylation and altered expression of the interferon regulatory factor 8 (IRF8) gene in non-small cell lung cancer (NSCLC). Pyrosequencing assays were performed on 191 tumor specimens from NSCLC patients. The changes in IRF8 mRNA expression, prior to and following treatment with a demethylating agent and methylation itself, were examined in 13 lung cancer cell lines by quantitative polymerase chain reaction (qPCR) and pyrosequencing.doi:10.3892/ol.2014.2234 pmid:25120651 pmcid:PMC4114630 fatcat:y77nidkglrcufder3xom33qkbi
more »... RF8 protein expression was examined in 94 of the 191 NSCLC specimens by immunohistochemical analysis. The IRF8 methylation level was significantly higher in the tumor tissues than in matched non-malignant lung tissues (P<0.0001). IRF8 was more frequently methylated in tumor tissues compared with matched non-malignant lung tissues, as defined by a predetermined cut-off value (P<0.0001). The IRF8 methylation level was strongly correlated with the change in mRNA expression in lung cancer cell lines and with the protein expression level in primary tumors. The IRF8 gene was more frequently methylated in patients without an epidermal growth factor receptor (EGFR) mutation than in patients with an EGFR mutation (P=0.015). IRF8 methylation correlated with recurrent prognosis in adenocarcinomas (log-rank test, P=0.048). IRF8 protein expression was frequently silenced in males, smokers, patients with non-adenocarcinoma or with wild-type EGFR, or in an advanced stage. IRF8 is often silenced by its methylation, which is a frequent event in NSCLC and, therefore, methylation of IRF8 may act as a prognostic marker for recurrence. Analysis of IRF8 methylation status may provide novel opportunities for improved prognosis and therapy of resected NSCLC.
Recently, droughts have become widespread in the Northern Hemisphere, including in Mongolia. The ground surface condition, particularly vegetation coverage, affects the occurrence of dust storms. The main sources of dust storms in the Asian region are the Taklimakan and Mongolian Gobi desert regions. In these regions, precipitation is one of the most important factors for growth of plants especially in arid and semi-arid land. The purpose of this study is to clarify the relationship betweendoi:10.3390/land7010019 fatcat:aq437axhirbjnb6ysaliwjhp3u
more »... ipitation and vegetation cover dynamics over 29 years in the Gobi region. We compared the patterns between precipitation and Normalized Difference Vegetation Index (NDVI) for a period of 29 years. The precipitation and vegetation datasets were examined to investigate the trends during 1985-2013. Cross correlation analysis between the precipitation and the NDVI anomalies was performed. Data analysis showed that the variations of NDVI anomalies in the east region correspond well with the precipitation anomalies during this period. However, in the southwest region of the Gobi region, the NDVI had decreased regardless of the precipitation amount, especially since 2010. This result showed that vegetation in this region was more degraded than in the other areas.
Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectabledoi:10.1002/stem.1300 pmid:23225744 pmcid:PMC4043220 fatcat:c6zoakfoyjdwrao7gk6l4r7hhe
more »... red bone (TEB) composed of mesenchymal stem cells (MSCs) and platelet-rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out large-scale clinical studies in patients with long-term follow-up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months were significantly increased over the preoperation baseline (p < .001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during longterm follow-up. Injectable TEB restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering.
In the Mongolian Plateau, the desert steppe, mountains, and dry lake bed surfaces may affect the process of dust storm emissions. Among these three surface types, dry lake beds are considered to contribute a substantial amount of global dust emissions and to be responsible for "hot spots" of dust outbreaks. The land cover types in the study area were broadly divided into three types, namely desert steppe, mountains, and dry lake beds, by a classification based on Normalized Difference Waterdoi:10.3390/land6040088 fatcat:wmewhjbfbbd3djog36234b5one
more »... x (NDWI) calculated from MODIS Terra satellite images, and Digital Elevation Model (DEM). This dry lake beds extracting method using remote sensing offers a new technique for identifying dust hot spots and potential untapped groundwater in the dry lands of the Gobi region. In the study area, frequencies of dry lake bed formation were calculated during the period of 2001 to 2014. The potential dry lake area corresponded well with the length of the river network based on hydrogeological characterization (R 2 = 0.59, p < 0.001). We suggest that the threshold between dry lake bed areas and the formation of ephemeral lakes in semi-arid regions is eight days of total precipitation.
Jibi Inkoka Tembo
LONG-TERM OUTCOME OF PATIENTS WITH KAWASAKI DISEASE Kenji Waki, Kiyoshi Baba, Yoshio Arakaki, Shin-ichiro Yoshimura Division of Pediatrics, Heart Institute, Kurashiki Central Hospital, Kurashiki, Japan ...doi:10.1203/00006450-200301000-00191 fatcat:p4qdgp4bsnco3pocmfj6glf7d4
Genome-wide DNA hypomethylation and gene hypermethylation play important roles in instability and carcino genesis. Methylation in long interspersed nucleotide element 1 (LINE-1) is a good indicator of the global DNA methylation level within a cell. Slit homolog 2 (SLIT2), myelin and lymphocyte protein gene (MAL) and insulin-like growth factor binding protein 7 (IGFBP7) are known to be hypermethylated in various malignancies. The aim of the present study was to assess the precise methylationdoi:10.3892/or.2013.2266 pmid:23381221 pmcid:PMC3621652 fatcat:mzr3gk6fjbgnxl7bnkeokbw53a
more »... ls of LINE-1, SLIT2, MAL and IGFBP7 in non-small cell lung cancer (NSCLC) using a pyrosequencing assay. Methylation of all regions was examined in 56 primary NSCLCs using a pyrosequencing assay. Changes in mRNA expression levels of SLIT2, MAL and IGFBP7 were measured before and after treatment with a demethylating agent. Methylation of these genes was also examined in 9 lung cancer cell lines using RT-PCR and a pyrosequencing assay. Frequencies of hypomethylation of LINE-1 and hypermethylation of SLIT2, MAL and IGFBP7, defined by predetermined cut off values, were 55, 64, 46 and 54% in NSCLCs, respectively and exhibited tumor-specific features. The hypermethylation of all genes was well correlated with changes in expression. The methylation level and frequency of MAL were significantly higher in smokers and in patients without EGFR mutations. Through accurate measurement of methylation levels using pyrosequencing, hypomethylation of LINE-1 and hypermethylation of SLIT2, MAL and IGFBP7 were frequently detected in NSCLCs and associated with various clinical features. Analysis of the methylation profiles of these genes may, therefore, provide novel opportunities for the therapy of NSCLCs. Abbreviations: LINE-1, long interspersed nucleotide element 1; SLIT2, slit homolog 2; MAL, myelin and lymphocyte protein gene; IGFBP7, insulin-like growth factor binding protein 7; NSCLC, non-small cell lung cancer
, DDS, PhD a,p , Kenji Fueki, DDS, PhD a,q , Eri Makihara, DDS, PhD a,r , Sawako Yokoyama, DDS, PhD a,i , Shuji Ohkawa, DDS, PhD e,s , Kazuyoshi Baba, DDS, PhD b,i and Tetsuo Ichikawa, DDS, PhD f,t 学生技能 ... , Takashi Sawase, DDS, PhD d,j , Shuichiro Yamashita, DDS, PhD c,k , Enami-Tanaka Yumiko, DDS l , Yasunori Ayukawa, DDS, PhD a,m , Takayuki Ueda, DDS, PhD a,n , Hiroshi Egusa, DDS, PhD a,o , Shunsuke Baba ...doi:10.2186/ajps.12.5 fatcat:qztc7vbdjvf3jcp3yixoq2b76q
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