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Fc-sialylated IgGs are rare in humans as well as in industrial host cell lines such as Chinese hamster ovary (CHO) cells. ... method for the efficient Fc glycan a2,6sialylation of a wild-type and a F243A IgG1 mutant by transient co-expression with the human a2,6-sialyltransferase 1 (ST6) and b1,4-galactosyltransferase 1 (GT) in CHO ... Materials and Methods Cell line CHO cells expressing a truncated Epstein-Barr virus Nuclear Antigen-1 (CHO-3E7) were grown in suspension in serum-free FreeStyle TM F17 medium (Invitrogen, cat# A13835 ...doi:10.1080/19420862.2015.1029215 pmid:25875452 pmcid:PMC4622614 fatcat:qfexay2ncvgvdggkjwxf5lww24
Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype microRNA-mediated engineering of CHO cells is an attractive method for enhancing various industrially ... CHO clones depleted of miR-23 using a miR-sponge decoy demonstrated an average ~3-fold enhanced specific productivity with no impact on cell growth. ...doi:10.1002/biot.201500101 pmid:26097147 fatcat:it4xxhy5xjh2fomcunexmtx55e
In order to reach yields closer to 200 mg/L, stable pools of CHO cells expressing GT, ST6 and an IgG1 were selected. ... The Fc domain of mabs produced in CHO typically possesses N-glycans with low galactosylation and very low sialylation (0-2% of α2,3-sialylated glycans). ...doi:10.1186/1753-6561-9-s9-o3 fatcat:pt2izikr25g57n5vzacqwxh45u
., 1997; Cho et al., 2002c,d) and neuroendocrine cells (Cho et al., 2002a,b) examined, at near nm resolution and in real time. ... As eluded earlier resting GH secreting cells of the pituitary gland (Cho et al., 2002a) and chromaffin cells of the adrenal medulla (Cho et al., 2002b) , both revealed the presence of pits and depressions ...doi:10.1016/s0006-3495(03)74631-1 pmid:12944316 pmcid:PMC1303375 fatcat:7fowm4b34vf3zejpssldbiapw4
A pilot study using portable GPS (global positioning system) units concurrently with the diary showed moderate to high agreement for the number of walk trips taken during the week (Cho GH, personal communication ...doi:10.1186/1476-072x-8-62 pmid:19925658 pmcid:PMC2789060 fatcat:awnzdg3tzvborflln6w7ydu7b4
The structure and arrangement of the SNARE complex formed as a result, and any changes in capacitance or conductance were recorded using AFM (Schneider et al., 1997; Cho et al., 2002a,b,c) and a bilayer ... The KCl gradient provided a driving force for vesicle incorporation that was independent of the presence of SNARE proteins (Kelly and Woodbury, 1996) . ...doi:10.1016/s0006-3495(02)75263-6 pmid:12414686 pmcid:PMC1302338 fatcat:3dxhmylwebdqzdoeqjyraxnxpq
We utilised the expression data in conjunction with in-silico tools to identify potential miRNA-mediated regulation of mRNA/proteins involved in CHO cell growth rate. ... Conclusions: The evidence presented in this study indicates that biological processes such as mRNA processing and protein synthesis are correlated with growth rate in CHO cells. ... The recent publication of the CHO genome  along with sequence from similar initiatives [22, 23] promises to facilitate analyses at all levels of the CHO biological system not only through direct ...doi:10.1186/1471-2164-13-656 pmid:23170974 pmcid:PMC3544584 fatcat:lzsc3sqlyvenzc6624j4z7p42a
Distributed systems designs often employ replication to solve two different kinds of availability problems. First, to prevent the loss of data through the permanent destruction or disconnection of a distributed node, and second, to allow prompt retrieval of data when some distributed nodes respond slowly. For simplicity, many systems further handle crash-restart failures and timeouts by treating them as a permanent disconnection followed by the birth of a new node, relying on peer replicationdblp:conf/usenix/YooKKCP12 fatcat:z7l6z5bm55aytoow2hmju2uxiu
more »... ther than persistent storage to preserve data. We posit that for applications deployed in modern managed infrastructures, delays are typically transient and failed processes and machines are likely to be restarted promptly, so it is often desirable to resume crashed processes from persistent checkpoints. In this paper we present MaceKen, a synthesis of complementary techniques including Ken, a lightweight and decentralized rollback-recovery protocol that transparently masks crash-restart failures by careful handling of messages and state checkpoints; and Mace, a programming toolkit supporting development of distributed applications and application-specific availability via replication. MaceKen requires near-zero additional developer effort-systems implemented in Mace can immediately benefit from the Ken protocol by virtue of following the Mace execution model. Moreover, this model allows multiple, independently developed application components to be seamlessly composed, preserving strong global reliability guarantees. Our implementation is available as open source software.
and Kelly, supra note 18 at 497-98. 98 100 Id., at 624. 101 Id., at 624-25. 102 Id., at 625. ... See also Sungjoon Cho, The Nature of Remedies in International Trade Law, 65 U. PITT. L. ...doi:10.2139/ssrn.2088368 fatcat:d36avbfugre2tod4rvxveqodya
The results reported in this paper contribute to the debate about gender skill gaps in at least three ways. First, we document the large differences in early gender gaps across developed countries using a large scale, modern, representative data source. Second, we show that countries with pro-female sorting, countries that place girls in classes with higher than average scores have smaller gender test score gaps, at least in math. Third, we show that the degree of academic tracking isdoi:10.1016/j.econedurev.2009.10.015 fatcat:23yoqcarmvapdiagcrwvp2ft6q
more »... with observed gender gaps across developed countries.
Conclusions: Within the limits of this study, administration of this CHO-derived surrogate of ponezumab to 18-month-old amyloid ß-bearing Tg2576 mice for up to 26 weeks did not result in an increase in ... P3-014 DEVELOPMENT OF PRECLINICAL MODELS OF VASOGENIC EDEMA (VE) AND MICROHEMORRHAGE (MCH) USING IN-VIVO BIOIMAGING AND HISTOPATHOLOGY Gary Freeman 1 , Thomas Brown 1 , James Goodman 2 , Kelly Bales 1 ...doi:10.1016/j.jalz.2011.05.1452 fatcat:aihcgodk65arlicvyqodrieroa
Kelly. 1994. Proc. Natl. Acad. Sci. USA. 91: 12750-12754.) . ... cose transporter, is examined in transfected CHO cells. ...doi:10.1083/jcb.140.3.565 pmid:9456317 pmcid:PMC2140164 fatcat:vxze672uxfcqxjkcntd7st5j6y
However, no spectral library for CHO cells is publicly available. ... Here we present a comprehensive CHO global spectral library to measure the abundance of more than 10,000 proteins consisting of 199,102 identified peptides from a CHO-K1 cell proteome. ... two CHO cell lines, namely CHO-DG44 and CHO-S. ...doi:10.1038/s41597-020-00594-z pmid:32782267 fatcat:y27dh5esbbctbfwtrysmythk2u
We transfected Chinese hamster ovary (CHO) cells with a cloned v-mos gene (pHT25). The mos family of oncogenes has previously been shown to have serine-threonine kinase activity. ... The characteristics of altered morphology and depressed growth rate were reminiscent of changes seen after the activation of the cyclic AMP-dependent protein kinase (PKA) in CHO cells. ... All CHO cell lines were grown as described previously (16) . ...doi:10.1128/mcb.8.11.4685 pmid:2974919 pmcid:PMC365558 fatcat:vkonanzysbckfjuvbthzwjluou
Background-In patients undergoing pancreatic resection (PR), identification of subgroups at increased risk for postoperative complications can allow focused interventions that may improve outcomes.doi:10.1007/s11605-013-2399-7 pmid:24277570 pmcid:PMC4804699 fatcat:5pweeoo5l5hkvjob3im2oldowa
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