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Production of offspring from a germline stem cell line derived from neonatal ovaries

Kang Zou, Zhe Yuan, Zhaojuan Yang, Huacheng Luo, Kejing Sun, Li Zhou, Jie Xiang, Lingjun Shi, Qingsheng Yu, Yong Zhang, Ruoyu Hou, Ji Wu
2009 Nature Cell Biology  
Figure S1 Putative FGSCs in day 5 and adult mouse ovaries. (a) Dual immunofluorescence for MVH (red) and BrdU (green) in day 5 ovary. Arrows indicate putative FGSCs in ovarian surface epithelium. (b, c) Dual immunofluorescence for MVH (red) and BrdU (green) (b) corresponding to histological analysis of the section (c). Arrow in (b) indicates putative FGSC corresponding to its morphology (arrow in c) in neonatal ovarian surface epithelium. (d) Dual immunofluorescence for MVH (red) and BrdU
more » ... ) in adult ovary. Arrow indicates putative FGSC undergoing mitosis in ovarian surface epithelium. (e, f) Dual immunofluorescence for MVH (red) and BrdU (green) (e) corresponding to histological analysis of the section (f). Arrow in (e) denotes putative FGSC corresponding to its morphology (arrow in f) in adult ovarian surface epithelium. Scale bars, 10 µm. d e f a b c
doi:10.1038/ncb1869 pmid:19363485 fatcat:rd2l4zalgjd6fekllyng4p56ge

Production of transgenic mice by random recombination of targeted genes in female germline stem cells

Yong Zhang, Zhaojuan Yang, Yunze Yang, Shuzeng Wang, Lingjun Shi, Wenhai Xie, Kejing Sun, Kang Zou, Lei Wang, Ji Xiong, Jie Xiang, Ji Wu
2010 Journal of Molecular Cell Biology  
Oocyte production in most mammalian species is believed to cease before birth. However, this idea has been challenged with the finding that postnatal mouse ovaries possess mitotically active germ cells. A recent study showed that female germline stem cells (FGSCs) from adult mice were isolated, cultured long term and produced oocytes and progeny after transplantation into infertile mice. Here, we demonstrate the successful generation of transgenic or gene knock-down mice using FGSCs. The FGSCs
more » ... rom ovaries of 5-day-old and adult mice were isolated and either infected with recombinant viruses carrying green fluorescent protein, Oocyte-G1 or the mouse dynein axonemal intermediate chain 2 gene, or transfected with the Oocyte-G1 specific shRNA expression vector (pRS shOocyte-G1 vector), and then transplanted into infertile mice. Transplanted cells in the ovaries underwent oogenesis and produced heterozygous offspring after mating with wild-type male mice. The offspring were genetically characterized and the biological functions of the transferred or knock-down genes were investigated. Efficiency of genetransfer or gene knock-down was 29% -37% and it took 2 months to produce transgenic offspring. Gene manipulation of FGSCs is a rapid and efficient method of animal transgenesis and may serve as a powerful tool for biomedical science and biotechnology.
doi:10.1093/jmcb/mjq043 pmid:21149239 fatcat:fms4nfwyfbbgloouxrub345jfa

Distributed Analysis in CMS

Alessandra Fanfani, Anzar Afaq, Jose Afonso Sanches, Julia Andreeva, Giusepppe Bagliesi, Lothar Bauerdick, Stefano Belforte, Patricia Bittencourt Sampaio, Ken Bloom, Barry Blumenfeld, Daniele Bonacorsi, Chris Brew (+67 others)
2010 Journal of Grid Computing  
The CMS experiment expects to manage several Pbytes of data each year during the LHC programme, distributing them over many A. Fanfani et al. location for physics analysis to support a wide community with thousands potential users. This represents an unprecedented experimental challenge in terms of the scale of distributed computing resources and number of user. An overview of the computing architecture, the software tools and the distributed infrastructure is reported. Summaries of the
more » ... ce in establishing efficient and scalable operations to get prepared for CMS distributed analysis are presented, followed by the user experience in their current analysis activities.
doi:10.1007/s10723-010-9152-1 fatcat:jvs2ccg7r5em7b3vringhmobye

Author Index

2021 2021 International Conference on Digital Society and Intelligent Systems (DSInS)  
10.1109/DSInS54396.2021.9670554 Cao, Kejing  ...  .......................... 292 Zhang, Jiaxiang ............................................... 300 Wang, Feiyu..................................................... 67 Zhang, Kang  ... 
doi:10.1109/dsins54396.2021.9670554 fatcat:o6fcyjkh5vbe5o526la5znre3q

Extraction and Antioxidant Activity Evaluation of Polyphenols of Brown Rice Tea from Japonica Rice and Indica Rice

Shuangqi Tian, Zhicheng Chen, Kejing Yang
2020 Journal of Nutritional Health & Food Science  
Figure 1 : 1 Experimental Design purchased from Yunnan Puqi Kang Tea Co., Ltd. (Yunnan, China). Burdock tea was purchased from China's burdock village, Lanling (Shandong, China).  ...  Page 3 of 6 6 Citation: Zhicheng Chen, Shuangqi Tian, Kejing Yang (2020) Extraction and Antioxidant Activity Evaluation of Polyphenols of Brown Rice Tea from Japonica Rice and Indica Rice.  ... 
doi:10.15226/jnhfs.2020.001172 fatcat:hrr24gpluvhbfgw2ro6p6lpkqu

Fabrication of an ordered mesoporous nanoparticle SiO2/Mc and their CMP of fused silica application

L Xu, C L Zou, X Zhang, C X Kang, G H Luo, G S Pan
2018 IOP Conference Series: Materials Science and Engineering  
The wafer was processed with different size of SiO 2 abrasives via a UNIPOL 1000S machine from Kejing Co., Ltd under the conditions of the pressure 64g/cm 2 , slurry flow rate 20 mL/min, carrier plate  ... 
doi:10.1088/1757-899x/307/1/012028 fatcat:jjexollu7rdmrf4723coycqj5y

On the monophthongization of /ɨi/ in Seoul Corpus

박선우, 한혜승, Cho Sung-Moon
2016 Studies in Phonetics Phonology and Morphology  
이 러한 문제점을 해결하려면 Kang (1999)와 같이 현지답사를 통하여 자연발화 를 녹음하고 전사하거나 자연발화를 담은 음성코퍼스를 분석해야 한다. 본 연구는 40시간 분량의 자연발화를 담고 있는 '서울코퍼스'(Yun et al. 2015)로부터 /ɨi/가 포함된 2,370개의 음성자료를 추출하고 /ɨi/의 단모음화 를 분석한 결과이다.  ...  일부 단어만을 조사하였으나, 음절두음을 갖는 경우('희망'), 비어두 환 경('논의'), 관형격조사('우리+의')에서 단모음화가 우세한 경향은 Kang (1999) (5) 단모음화의 양상 분석에서 제외한 어절 걔의 [keje] 거의 [kəwi] 거의 [koju] 국회의원 [kukʰeən] 그들의 [kɨdɨɾje] 나라의 [naɾe] 때의 [t'eje  ...  위의 도표는 Kang (1999: 31-34 (2) /ɨi/의 단모음화 결과와 비율 (조성문 2005) 위의 도표는 조성문(2005: 385-391)의 논의 결과를 요약하여 정리한 것이 다.  ... 
doi:10.17959/sppm.2016.22.1.35 fatcat:ehqqqfqefve25kb4v62fsbsr64

Synthesis and Electrochemical Performance of LiMnPO4by Hydrothermal Method

Daichi Fujimoto, Yu Lei, Zheng-Hong Huang, Feiyu Kang, Junichi Kawamura
2014 International Journal of Electrochemistry  
LiMnPO 4 /C compound was mixed with PVDF (polyvinyliden fluoride: Shenzhen Kejing Star Technology Company) and carbon black (Shenzhen Kejing Star Technology Company) and then The solution was then pasted  ...  Sample Mn2p 3/2 [eV] Sample A 641.58 Sample B 641.57 Sample C 642.04 Sample D 641.99 dispersed in NMP (N-methylpyrrolidone: Shenzhen Kejing Star Technology Company).  ... 
doi:10.1155/2014/768912 fatcat:3v2kbl6mobgrpnqv673dxxocla

Survey and Analysis of Literature in "E-waste Management"

Swapnali Lotlikar
2017 International Journal Of Mechanical Engineering And Information Technology  
Hai-Yong Kang and Julie M.  ...  [36] [Kejing Zhang 2010] has developed STOF model for Ewaste recycling service system. [Hua ZHONG ,et-al, 2011 ] has proposed framework for deposit refund system .  ... 
doi:10.18535/ijmeit/v5i1.01 fatcat:hlwngpudd5h73pcjytyb3notiu

A rapid and durable response to cabozantinib in an osimertinib- resistant lung cancer patient with MET D1228N mutation: a case report

Yukun Kuang, Jiyu Wang, Peihang Xu, Yifan Zheng, Lihong Bai, Xue Sun, Zimu Li, Runjing Gan, Huixia Li, Zunfu Ke, Kejing Tang
2021 Annals of Translational Medicine  
In a recent case reported by Kang et al., one patient benefited from the combination of osimertinib and cabozantinib after simultaneous development of secondary MET mutations upon crizotinib treatment  ... 
doi:10.21037/atm-21-3861 pmid:34532491 pmcid:PMC8422132 fatcat:2nw2bcn2jjglvmw5hwgfaznbnm

Mutation Analysis of Isocitrate Dehydrogenase in Acute Lymphoblastic Leukemia

Yiqun Zhang, Hui Wei, Kejing Tang, Dong Lin, Cuiping Zhang, Yingchang Mi, Lin Wang, Cuicui Wang, Min Wang, Jianxiang Wang
2012 Genetic Testing and Molecular Biomarkers  
Kang et al. (2009) reported one B-acute lymphoblastic leukemia (B-ALL) case with an IDH1 R132C mutation (1/60; 1.7%).  ...  Only three IDH-mutated cases were so far identified in ALL (Kang et al., 2009; Abbas et al., 2010; Andersson et al., 2011) . Our observation confirmed the occurrence of IDH1 mutation in ALL.  ... 
doi:10.1089/gtmb.2011.0323 pmid:22809434 pmcid:PMC3422558 fatcat:xpo3fsgck5ccxadon2d7urg2jy

"Ngasag" di dalam Geguritan Gambaran Konflik Nilai Budaya Tani Jawa

Dhanu Priyo Prabowo
2017 Atavisme  
Ajaran Jawa melarang seseorang berlaku pelit karena seseorang yang su-dah diberi predikat pelit 'cethil' adalah orang yang sudah dicereni 'dicatat keje-lekannya' oleh masyarakat lingkungan-nya (Santosa  ...  , temanku' ngindhit obor ngindhit dhunak--e 'membawa obor membawa tenggok besar' isi bakiyak nanting capinge 'berisi bakiyak membawa capingnya' kawit parak esuk 'sejak pagi menjelang' nglarag gentine kang  ...  Akan tetapi, sebagai pelaku ngasag, me-reka tidak pernah menengok ke bela-kang atau menyesali hasil karyanya di-ambil oleh yang empunya sawah (wong kang winasis).  ... 
doi:10.24257/atavisme.v20i1.304.98-109 fatcat:2knelxvhlbe5fchnudk7367uhu

Issue Information

2021 Journal of food processing and preservation  
with ultrahigh pressure pretreatments on color, moisture characteristic, tissue structure, and sensory quality of freeze-dried strawberry slices Lihui Zhang, Yu Qiao, Chao Wang, Li Liao, Defang Shi, Kejing  ...  An, and Jianzhong Hu e15200 Effective rebaudioside a separation from stevia extracts by enzymatic bioconversion Ha-Nul Lee, Seong-Jin Hong, HyeJin Kang, Jung-Hyun Ju, Bo-Ram Park, Jin-A Ko, and Young-Min  ... 
doi:10.1111/jfpp.14612 fatcat:ay3hpa2crbgtphad4rqyxg3lbi

Experimental process and kinetic behavior of carbothermal reduction of lithium cobaltate as a cathode for waste lithium batteries

Cao Zhi-Kang, Li Ji-Dong, Li Zhen, Wang Xue-Lian, Yue Ling-Feng
2021 Materials Express  
The carbothermal reduction experiment device was a vacuum tube high-temperature sintering furnace (Hefei Kejing Material Technology Co., Ltd., OTF-1000X).  ...  207.241.225.157 On: Fri, 21 Jan 2022 04:05:25 Copyright: American Scientific Publishers Delivered by Ingenta Materials Express Experimental process and kinetic behavior of carbothermal reduction Zhi-Kang  ... 
doi:10.1166/mex.2021.2116 fatcat:rfsvofiic5f5lhhwonddtbfjki

HASIL BELAJAR PESERTA DIDIK KELAS V SD NEGERI 9 LAHAT PADA TEMA PERISTIWA DALAM KEHIDUPAN MELALUI PENERAPAN MEDIA AUDIO VISUAL

Madian Anggraini, Toybah Toybah, Sugiono Sugiono
2021 Jurnal Inovasi Sekolah Dasar  
JURNAL INOVASI SEKOLAH DASAR VOLUME 8, NOMOR 2, NOVEMBER 2021 Berdasarkan uraian latar bela-kang yang telah dikemukakan di atas, peneliti terdorong untuk melakukan pene-litian yang diberi judul "Upaya  ...  penggunaan media audio visual yang dikemukakan Sanaky (2015:123) , yaitu: (1) memiliki daya tarik tersendiri yang dapat menjadi pemicu atau memotivasi peserta didik untuk belajar, (2) dapat mengatasi keje-nuhan  ... 
doi:10.36706/jisd.v8i2.15856 fatcat:dszrmqzhrvektdu6udm47lqgdi
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