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Maes, ed., Joris Helleputte. Architekt en politicus 1852-1925, I, Biografie, II, Oeuvrecatalogus (Reeks KADOC-Artes I; Leuven: Universitaire pers, KADOC, 1998, 296 en 288 blz., ISBN 90 6186 863 7). ... De loopbaan van de Vlaming Joris Helleputte ...doi:10.18352/bmgn-lchr.5124 fatcat:jltj4gd2f5e2na7mcbnryt65mq
An overview is given on the state-of-the-art in synthesis of sequence-controlled and sequence-defined oligomers and polymers.doi:10.1039/c8py01190g fatcat:cgfkcrjd55asph7g3i27l24c34
The ccd addiction system plays a crucial role in the stable maintenance of the Escherichia coli F plasmid. It codes for a stable toxin (CcdB) and a less stable antidote (CcdA). Both are expressed at low levels during normal cell growth. Upon plasmid loss, CcdB outlives CcdA and kills the cell by poisoning gyrase. The interactions between CcdB, CcdA, and its promoter DNA were analyzed. In solution, the CcdA-CcdB interaction is complex, leading to various complexes with different stoichiometry.doi:10.1074/jbc.m105505200 pmid:11741897 fatcat:l3obi66kizggxjuisv37svx7vu
more »... dA has two binding sites for CcdB and vice versa, permitting soluble hexamer formation but also causing precipitation, especially at CcdA:CcdB ratios close to one. CcdA alone, but not CcdB, binds to promoter DNA with high on and off rates. The presence of CcdB enhances the affinity and the specificity of CcdA-DNA binding and results in a stable CcdA⅐ CcdB⅐DNA complex with a CcdA:CcdB ratio of one. This (CcdA 2 CcdB 2 ) n complex has multiple DNA-binding sites and spirals around the 120-bp promoter region.
Manipulation of high-density materials, such as crystals and liquid condensates, is of great importance for many applications, including serial crystallography, structural and molecular biology, chemistry, and medicine. In this work, we describe an acoustic technique to focus and harvest flowing crystals and liquid condensates. Moreover, we show, based on numerical simulations, that the acoustic waves can be used for size-based particle (crystals, droplets, etc.) separation. This is andoi:10.3390/cryst9030120 fatcat:rugjfuons5gl3iu6tiy4ufvvtu
more »... technological step in biological research, medical applications, and industrial processes. The presented technology offers high precision, biocompatibility, ease of use and additionally, is non-invasive and inexpensive. With the recent advent of X-ray Free Electron Laser (XFEL) technology and the associated enormous importance of a thin jet of crystals, this technology might pave the way to a novel type of XFEL injector.
Physics Letters B
Motivated by the results in hep-th/0508228, we perform a careful analysis of the allowed linear constraints on N=(2,2) scalar superfields. We show that only chiral, twisted-chiral and semi-chiral superfields are possible. Various subtleties are discussed.doi:10.1016/j.physletb.2006.10.002 fatcat:ah3iil52vbdf3hlq427q7ws5ci
BMC Public Health
Although alcohol is socially accepted in most Western societies, studies are clear about its associated negative consequences, especially among university and college students. Studies on the relationship between alcoholrelated consequences and both beverage type and drinking onset, however, are scarce, especially in a European context. The aim of this research was, therefore, twofold: (1) What is the relationship between beverage type and the negative consequences experienced by students? anddoi:10.1186/s12889-018-5120-7 pmid:29433466 pmcid:PMC5809947 fatcat:ahgjktceprcp7gfkdo3ablicoi
more »... 2) Are these consequences determined by early drinking onset? We will examine these questions within the context of a wide range of alcohol-related consequences. Methods: The analyses are based on data collected by the inter-university project 'Head in the clouds?', measuring alcohol use among students in Flanders (Belgium). In total, a large dataset consisting of information from 19,253 anonymously participating students was available. Negative consequences were measured using a shortened version of the Core Alcohol and Drug Survey (CADS_D). Data were analysed using negative binomial regression. Results: Results vary depending on the type of alcohol-related consequences: Personal negative consequences occur frequently among daily beer drinkers. However, a high rate of social negative consequences was recorded for both daily beer drinkers and daily spirits drinkers. Finally, early drinking onset was significantly associated with both personal and social negative consequences, and this association was especially strong between beer and spirits drinking onset and social negative consequences. Conclusions: Numerous negative consequences, both personal and social, are related to frequent beer and spirits drinking. Our findings indicate a close association between drinking beer and personal negative consequences as well as between drinking beer and/or spirits and social negative consequences. Similarly, early drinking onset has a major influence on the rates of both personal and social negative consequences. The earlier students started drinking, the more negative consequences they experienced during college or university. Several (policy) interventions are discussed. This study is the first to incorporate detailed information on both beverage type and drinking onset, and its associated negative consequences, as measured by the CADS_D, in a large student population.
Understanding the kinetics and underlying physicochemical forces of liquid-liquid phase separation (LLPS) is of paramount importance in cell biology, requiring reproducible methods for the analysis of often severely aggregation-prone proteins. Frequently applied approaches, such as dilution of the protein from an urea-containing solution or cleavage of its fused solubility tag, however, often lead to very different kinetic behaviors. Here we suggest that at extreme pH values even proteins suchdoi:10.1101/563700 fatcat:q5k7zrs7g5c6vnrlpdq6spvnla
more »... s the low-complexity domain (LCD) of hnRNPA2, TDP-43, and NUP-98 can be kept in solution, and then their LLPS can be induced by a jump to native pH, resulting in a system that can be easily controlled. This approach represents a generic method for studying LLPS under near native conditions, providing a platform for studying the phase-separation behavior of diverse proteins.
2013;8(9):303. Cardiologia CROATICA Objectives: VT ablations could benefit from dynamic 3D (i.e. 4D) visualisation of the left ventricle (LV) as roadmap for anatomy-guided procedures, e.g. by means of rotational angiography (3DRA). To limit radiation burden, low-dose, noisy 3DRA image datasets have to be used. Our aim was to develop an algorithm combining information of several cardiac phases to filter out noise, enabling accurate semi-automatic segmentation (SAS) and generation of multi-phasedoi:10.15836/ccar.2013.303 fatcat:tgqu3dmsx5cjzphajzn32yep24
more »... egmentation surfaces. Methods and Results: We explored non-rigid interphase registration (IPR) using image warping and subsequent image averaging of 4 cardiac phases in low-dose 3DRA images from 5 porcine experiments, acquired with a novel protocol of slow atrial pacing. IPR parameter settings were optimised against manual delineations of the LVs using a score (Q) composed of standardised similarity measures. SAS was done for varying voxel intensity thresholds (ISO). Figure A
25 BACKGROUND: Binge drinking in higher education is an important problem. To target binge drinking 26 in students it is necessary to study the social context of students. Faculties (i.e., colleges or schools in 27 Northern American education) are social contexts in which students behave, but little is known about 28 how the faculty structure relates to monthly binge drinking. In this study, the relationship with 29 student-perceived binge drinking norms at faculty-level are investigated indoi:10.1016/j.addbeh.2016.03.011 pmid:27077965 fatcat:votykpj6cjbutlfmp2df3xsiby
more »... tion to known 30 personal determinants. 31 METHODS: Data were collected from 7,181 students in 22 faculty-level units, using an anonymous 32 online survey. Multilevel analyses were used to investigate the relationship of both individual-level 33 determinants (e.g., perceived norms, social drinking motives) and student-perceived binge drinking 34 norms at faculty-level on monthly binge drinking. 35 RESULTS: Two-third (62.2%) of the sample were female and the mean age was 21.06 (SD = 2.85) 36 years. In males, significant faculty-level variance in monthly binge drinking was found. At faculty-level 37 only same-sex student-perceived binge drinking norms showed a positive relationship (OR=2.581; 38 95%CI=[1.023,6.509]). At individual level, both opposite-and same-sex perceived binge drinking 39 norms and social drinking motives positively related to monthly binge drinking. In females, no 40 significant faculty-level variance was found. Only individual-level determinants positively related to 41 monthly binge drinking. No cross-level interactions were found. 42 CONCLUSION: Besides individual determinants faculties are, especially in men, relevant 43 environmental structures and networks to take into account when targeting binge drinking in higher 44 education. 45 46 Keywords 47 Social environment, student, binge drinking, peer group, university, perceived norm 48
Aims Interventional left ventricular (LV) procedures integrating static 3D anatomy visualization are subject to mismatch with dynamic catheter movements due to prominent LV motion. We aimed to evaluate the accuracy of a recently developed acquisition and post-processing protocol for low radiation dose LV multi-phase rotational angiography (4DRA) in patients. Methods and results 4DRA image acquisition of the LV was performed as investigational acquisition in patients undergoing left-sideddoi:10.1093/ehjci/jev120 pmid:26003152 pmcid:PMC4882875 fatcat:i6uf7exsmzcwbkp3k3vu6ekxti
more »... n (11 men; BMI ¼ 24.7 + 2.5 kg/m 2 ). Iodine contrast was injected in the LA, while pacing from the RA at a cycle length of 700 ms. 4DRA acquisition and reconstruction were possible in all 11 studies. Reconstructed images were post-processed using streak artefact reduction algorithms and an interphase registration-based filtering method, increasing contrast-tonoise ratio by a factor 8.2 + 2.1. This enabled semi-automatic segmentation, yielding LV models of five equidistant phases per cardiac cycle. For evaluation, off-line 4DRA fluoroscopy registration was performed, and the 4DRA LV contours of the different phases were compared with the contours of five corresponding phases of biplane LV angiography, acquired in identical circumstances. Of the distances between these contours, 95% were ,4 mm in both incidences. Effective radiation dose for 4DRA, calculated by patient-specific Monte-Carlo simulation, was 5.1 + 1.1 mSv. Conclusion Creation of 4DRA LV models in man is feasible at near-physiological heart rate and with clinically acceptable radiation dose. They showed high accuracy with respect to LV angiography in RAO and LAO. The presented technology not only opens perspectives for full cardiac cycle dynamic anatomical guidance during interventional procedures, but also for 3DRA without need for very rapid pacing. ---
AbstractUnderstanding the kinetics, thermodynamics, and molecular mechanisms of liquid–liquid phase separation (LLPS) is of paramount importance in cell biology, requiring reproducible methods for studying often severely aggregation-prone proteins. Frequently applied approaches for inducing LLPS, such as dilution of the protein from an urea-containing solution or cleavage of its fused solubility tag, often lead to very different kinetic behaviors. Here we demonstrate that at carefully selecteddoi:10.1038/s42003-020-01596-8 pmid:33469149 fatcat:6pp3xrtc2jf2fjccs4rosqzddu
more »... H values proteins such as the low-complexity domain of hnRNPA2, TDP-43, and NUP98, or the stress protein ERD14, can be kept in solution and their LLPS can then be induced by a jump to native pH. This approach represents a generic method for studying the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behavior of diverse proteins.
VT ablations could benefit from Dynamic 3D (4D) left ventricle (LV) visualization as road-map for anatomy-guided procedures. We developed a registration-based method that combines information of several cardiac phases to filter out noise and artifacts in low-dose 3D Rotational Angiography (3DRA) images. This also enables generation of accurate multi-phase surface models by semi-automatic segmentation (SAS). The method uses B-spline non-rigid inter-phase registration (IPR) and subsequentdoi:10.1117/12.2043480 dblp:conf/miigp/WielandtsBENMH14 fatcat:wf5dt5svcjarpk2dfqrtkfnqia
more »... g of the registered 3DRA images of 4 cardiac phases, acquired with a slow atrial pacing protocol, and was validated on data from 5 porcine experiments. IPR parameter settings were optimized against manual delineations of the LVs using a composed similarity score (Q), dependent on DICE-coefficient, RMSDistance, Hausdorff (HD) and the percentage of inter-surface distances ≤3mm and ≤4mm. The latter are clinically acceptable error cut-off values. Validation was performed after SAS for varying voxel intensity thresholds (ISO), by comparison between models with and without prior use of IPR. Distances to the manual delineations at optimal ISO were reduced to ≤3mm for 95.6±2.7% and to ≤4mm for 97.1±2.0% of model surfaces. Improved quality was proven by significant mean Qincrease irrespective of ISO (7.6% at optimal ISO (95%CI 4.6-10.5,p<0.0001)). Quality improvement was more important at suboptimal ISO values. Significant (p<0.0001) differences were also noted in HD (-20.5%;95%CI -12.1%--29.0%), RMSD (-28.3%;95%CI -21.7%--35.0%) and DICE (1.7%;95%CI 0.9%-2.6%). Generating 4D LV models proved feasible, with sufficient accuracy for clinical applications, opening the perspective of more accurate overlay and guidance during ablation in locations with high degrees of movement.
Preeclampsia (PE) has been associated with placental dysfunction, resulting in fetal hypoxia, accelerated erythropoiesis, and increased erythroblast count in the umbilical cord blood (UCB). Although the detailed effects remain unknown, placental dysfunction can also cause inflammation, nutritional, and oxidative stress in the fetus that can affect erythropoiesis. Here, we compared the expression of surface adhesion molecules and the erythroid differentiation capacity of UCB hematopoieticdoi:10.3390/ijms20082038 fatcat:yo7srpuibjgipmbt76qiky3xyy
more »... ogenitor cells (HSPCs), UCB erythroid profiles along with the transcriptome and proteome of these cells between male and female fetuses from PE and normotensive pregnancies. While no significant differences were observed in UCB HSPC migration/homing and in vitro erythroid colony differentiation, the UCB HSPC transcriptome and the proteomic profile of the in vitro differentiated erythroid cells differed between PE vs. normotensive samples. Accordingly, despite the absence of significant differences in the UCB erythroid populations in male or female fetuses from PE or normotensive pregnancies, transcriptional changes were observed during erythropoiesis, particularly affecting male fetuses. Pathway analysis suggested deregulation in the mammalian target of rapamycin complex 1/AMP-activated protein kinase (mTORC1/AMPK) signaling pathways controlling cell cycle, differentiation, and protein synthesis. These results associate PE with transcriptional and proteomic changes in fetal HSPCs and erythroid cells that may underlie the higher erythroblast count in the UCB in PE.
The concept of social capital has been extensively used to explain the relationship between socioeconomic status (SES) and adolescent health and well-being. Much less is known about the specific mechanism through which social capital impacts the relationship. This paper investigates whether an individual's perception of community social capital moderates or mediates the association between SES and life satisfaction. Methods: This study employs cross-sectional data from the 2009-2010 Czechdoi:10.1186/s12939-016-0490-x pmid:27955660 pmcid:PMC5154096 fatcat:w5r7kjc44rgojlpde2l7z6r6ae
more »... Behaviour in School-Aged Children survey: a WHO Collaborative Cross-National Study (HBSC). A sample of 4425 adolescents from the 5 th , 7 th and 9 th grade (94.5% school response rate, 87% student response) was used to perform multilevel analysis. Results: We found that pupils' life satisfaction was positively related to both family affluence and perceived wealth. Moreover, we found the cognitive component of social capital to be positively associated with life satisfaction. Additionally, a significant interaction was found, such that the social gradient in life satisfaction was flattened when pupils reported high levels of perceived community social capital. Conclusions: The present findings suggest that community social capital acts as an unequal health resource for adolescents, but could potentially represent opportunities for public health policy to close the gap in socioeconomic disparities.
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