A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
doi:10.1111/cns.12133 pmid:23876019 fatcat:mxqizhnvebcrppvttuf7z56rai
Morphine is widely used in patients undergoing surgical operations and is also reported to mediate cardioprotection of preconditioning. The current study determined effects of morphine at therapeutic to pharmacologic concentrations on cardiac action potential, L-type Ca 2؉ current (I Ca.L ), delayed rectifier K ؉ current (I K ), and inward rectifier K ؉ current (I K1 ) in isolated rabbit ventricular myocytes. Methods: Ventricular myocytes were enzymatically isolated from rabbit hearts. Actiondoi:10.1097/00000542-200508000-00011 pmid:16052110 fatcat:pefsipggzbchdhjp6t2za2ppje
more »... tential and membrane currents were recorded in current and voltage clamp modes. Results: Morphine at concentrations from 0.01 to 1 M significantly prolonged cardiac action potential, and at 0.1 and 1 M slightly but significantly hyperpolarized the resting membrane potential. In addition, morphine at 0.1 M significantly augmented I Ca.L (at ؉10 mV) from 5.9 ؎ 1.9 to 7.3 ؎ 1.7 pA/pF (by 23%; P < 0.05 vs. control) and increased I K1 (at ؊60 mV) from 2.8 ؎ 1.0 to 3.5 ؎ 0.9 pA/pF (by 27%; P < 0.05 vs. control). Five M naltrindole (a selective ␦-opioid receptor antagonist) or 5 M norbinaltorphimine (a selective -opioid receptor antagonist) prevented the increase in I Ca.L induced by morphine, but 5 M CTOP (a selective -opioid receptor antagonist) did not. The three types of opioid antagonists did not affect the augmentation of I K1 by morphine. Morphine had no effect on I K . Conclusions: These results indicate that morphine prolongs action potential duration by increasing I Ca.L , an effect mediated by ␦and -opioid
E-mail: firstname.lastname@example.org (Corresponding author) ORCID iD: 0000-0001-7538-553X Sheng-Feng Qin received the Ph. D. degree in product design from University of Wales Institute, UK in 2000. ...doi:10.1007/s11633-018-1138-7 fatcat:mevpvwhzsrhgbefwwbi6xjjmj4
Interactions among tumor cells, stromal cells, and extracellular matrix compositions are mediated through cytokines during tumor progression. Our analysis of 132 known cytokines and growth factors in published clinical breast cohorts and our 84 patient-derived xenograft models revealed that the elevated connective tissue growth factor (CTGF) in tumor epithelial cells significantly correlated with poor clinical prognosis and outcomes. CTGF was able to induce tumor cell epithelial-mesenchymaldoi:10.18632/oncotarget.4659 pmid:26318291 pmcid:PMC4694834 fatcat:aduduj7dpfbtba3jlxq7swgf2m
more »... sition (EMT), and promote stroma deposition of collagen I fibers to stimulate tumor growth and metastasis. This process was mediated through CTGF-tumor necrosis factor receptor I (TNFR1)-IκB autocrine signaling. Drug treatments targeting CTGF, TNFR1, and IκB signaling each prohibited the EMT and tumor progression.
Self-organized critical states (SOCs) and stochastic oscillations (SOs) are simultaneously observed in neural systems, which appears to be theoretically contradictory since SOCs are characterized by scale-free avalanche sizes but oscillations indicate typical scales. Here, we show that SOs can emerge in SOCs of small size systems due to temporal correlation between large avalanches at the finite-size cutoff, resulting from the accumulation-release process in SOCs. In contrast, the criticaldoi:10.1103/physrevlett.116.018101 pmid:26799044 fatcat:xrj2b5nhqfgkjcwm22f5fi5oeq
more »... hing process without accumulation-release dynamics cannot exhibit oscillations. The reconciliation of SOCs and SOs is demonstrated both in the sandpile model and robustly in biologically plausible neuronal networks. The oscillations can be suppressed if external inputs eliminate the prominent slow accumulation process, providing a potential explanation of the widely studied Berger effect or event-related desynchronization in neural response. The features of neural oscillations and suppression are confirmed during task processing in monkey eye-movement experiments. Our results suggest that finite-size, columnar neural circuits may play an important role in generating neural oscillations around the critical states, potentially enabling functional advantages of both SOCs and oscillations for sensitive response to transient stimuli. Self-organized criticality  is a key concept for describing the emergence of complexity in many natural systems  . The fingerprint of self-organized critical states (SOCs), the power-law distribution of avalanche sizes, means that the activity has no characteristic scale in the thermodynamic limit. As excellent functional complex systems in nature, neural systems in the brain have been supposed to operate at SOCs. Indeed, SOCs of neuronal firing activity have been observed in experiments with electrode arrays [2-4] and have been studied intensively in computational models [2, 5, 6] . It has been shown that critical states have functional advantages for both the sensory system  and memory , and they play an important role in the development of neural systems  . On the other hand, stochastic oscillation (SO) in brain activity has been observed for more than 80 years  . Oscillations characterized by repetition of activities with typical scales are believed to be essential to brain functions, especially to provide timing, predictability, coherence, and integration in neural information processing  . Several different oscillation bands exist and appear in different states of the brain  . The synchronization between inhibitory neurons has been found to be crucial for gamma oscillations (30-70 Hz)  . Neural field models  indicated that resonance between thalamus and cortex can generate alpha oscillations (8-13 Hz). Despite many modeling studies, a commonly accepted mechanism of alpha rhythm is still lacking  . This slow oscillation is particularly obvious during the resting states without systematic external stimuli (i.e., eyes closed). It has been known through the Berger effect, or alpha blockade, for a long time that when observers open their eyes, the electroencephalography (EEG) alpha oscillations decrease in amplitude or disappear completely  . Such oscillations can also be suppressed during cognitive task processing, typically known as event-related desynchronization (ERD)  , apparently suggesting that alpha oscillations and suppression are due to synchronization and desynchronization processes. ERD has been widely used as a sensitive measure for brain-computer interfaces (see a review in Ref.  ). SOCs and SOs of neural systems are often studied in different models with the implicit assumption that different activities do not coexist  . However, experiments on PRL 116,
Earthquake tremor from neighbouring country had caused several cases of structural and non-structural damages toward the buildings in Malaysia. This issue had raised public attention about the safeness of the low-rise, mid-rise and high-rise building in the country. Buildings without proper seismic resistance design will collapse during the earth earthquake and people who could not evacuated from the building will be buried alive. Viscous Damper is one of the passive structural control systemsdoi:10.1051/e3sconf/202234703012 fatcat:2jup4tq6fvgnpd6tdiiszpgvrm
more »... n attenuating the seismic response on building. The damper utilized principle of fluid flow through orifices to create friction that turns the movement into heat energy to be release into atmosphere. This study is to investigate the effectiveness and behaviour of the mechanical shock absorber as damper in resisting seismic force. Three different type of dampers are being tested under 6 different loading displacement amplitude to measure the hysteresis loops and energy dissipation capability. The results showed that the extra features of the coil over in EX5 Kriss Wave damper with 17 kNmm had significantly increase the energy dissipation capability up to 5.6 time better than the lowest 3 kNmm APIDO type damper.
Purpose To investigate choroidal thickness (CT) and its associations in children in a school-based study. Methods The cross-sectional school-based Gobi Desert Children Eye Study included 1565 out of 1911 (81.9%) eligible children from all schools in the oasis region of Ejina in the Gobi Desert. A detailed ophthalmic examination was performed, including spectral-domain optical coherence tomography with enhanced depth imaging for CT measurement. (0.90±0.06;range:0.66,1.23) and ratio of CT(2500μmdoi:10.1371/journal.pone.0179579 pmid:28617854 pmcid:PMC5472304 fatcat:s7ft7ubwazhuji5mfcwnjpujne
more »... asal to fovea)/SFCT (0.70±0.13; range:0.28,1.23) decreased with older age (P = 0.01;and P = 0.001, respectively), while ratio of CT(1000μm temporal to fovea)/SFCT (1.02±0.06;range:0.56,1.37) and ratio of CT (2500μm temporal to fovea)/SFCT (0.99±0.11;range:0.54,1.84) increased with older age (both P<0.001). Time spent outdoors or indoors was not significantly associated with CTrelated parameter in multivariate analysis. Conclusions In contrast to SFCT in adults and despite elongating axial length, SFCT in children increased in cross-sectional analysis with older age (up to 11 years of age) and then started to decrease with further ageing. It suggests an increase in choroidal volume up to the age of 11 years. In children, the choroid was thickest at 1000μm temporal to the fovea, followed by the subfoveal region, and this difference significantly increased with older age. In contrast, CT nasal to the fovea in relationship to SFCT decreased with older age. CT was independent of lifestyle-associated parameters. Choroidal thickness in children PLOS ONE | https://doi.org/10.
The nanotubes generated within anodized aluminum oxide (AAO) were filled with Fe3O4 nanoparticles to form blue fluorescent and magnetic nanocomposites.doi:10.1039/c4cc01877j pmid:24931060 fatcat:juqbbpakzbgo3h5t67hlgkrt74
Wong: interpreted results; designed and supervised the project; and drafted and critically revised the manuscript. Dr. ... Wong is an employee of Baylor Miraca Genetics Laboratories, is a faculty member of BCM with joint appointment at BMGL, and serves on the editorial board of the journal Mitochondrion. Dr. ...doi:10.1212/nxg.0000000000000015 pmid:27066551 pmcid:PMC4807910 fatcat:omkwlbpfvzfobis3fvs534yec4
Chee Cheung http:// orcid. org/ 0000-0003-1216-729X Jasper Ka Wai Wong http:// orcid. org/ 0000-0003-4039-5468 Ian Y Wong http:// orcid. org/ 0000-0002-5328-1796 ... 0000-0003-0221-2325 Bonnie N K Choy http:// orcid. org/ 0000-0002-9740-3935 Jonathan Cheuk Hung Chan http:// orcid. org/ 0000-0002-0177-8178 Alex L K Ng http:// orcid. org/ 0000-0002-8321-5634 Janice Jing ...doi:10.1136/bjophthalmol-2020-318199 pmid:34016574 fatcat:nvylw6aft5bybmj2bf6tt4ifpy
Background: Focused cardiac ultrasound (FOCUS) examination using a portable device is increasingly used for bedside diagnosis of cardiovascular diseases. This is a 4-week pilot project aiming to teach medical students to perform FOCUS to detect valvular heart lesions. Methods: Patients undergoing routine transthoracic echocardiography (TTE) were recruited by third year medical students who performed physical examination (PE) and FOCUS after 6-hour training to detect significantdoi:10.1016/j.ijcha.2018.03.007 pmid:29946563 pmcid:PMC6016074 fatcat:cfouhtpfpjcsdeohm5iia3fzd4
more »... re) valvular lesions. Performance of FOCUS and PE was compared to TTE as reference using kappa statistics. Results: 10 medical students performed 212 PE and FOCUS on 107 patients with mean age 63.7 ± 14.9 years. TTE detected 126 significant valvular lesions of which FOCUS correctly identified 54 lesions (κ = 0.45) compared to 32 lesions by PE (κ = 0.28, p b 0.01). FOCUS was better than PE in identifying mitral stenosis (κ = 0.51 vs. 0.17), aortic stenosis (κ = 0.45 vs. 0.16) and tricuspid regurgitation (κ = 0.39 vs. 0.09, all p b 0.01). Students became more proficient in performing FOCUS examination with time. Conclusions: Teaching junior medical students to perform and interpret FOCUS was feasible after brief training and better than PE in detecting significant valvular lesions. Further studies are warranted to determine the utility of incorporating this new technology into mainstream medical training.
Rice is one of the major exposure pathways of arsenic (As) in the human food chain which threatens over half the global population. A series of greenhouse pot investigations were conducted to examine the effects of Si application on iron plaque formation, As uptake and rice grain As speciation in indica and hybrid rice genotypes with different radial oxygen loss (ROL) patterns. The results demonstrated that Si significantly increased root (p<0.05) and grain (p<0.001) biomass. Indica genotypesdoi:10.1016/j.envpol.2016.01.004 pmid:26840513 fatcat:5ufruotfuvgnjgojuunwsvbiki
more »... th higher ROL induced greater Fe plaque formation compared to hybrid genotypes (p<0.005) and sequestered more As in Fe plaque. Silicon applications significantly increased Fe concentrations in iron plaque of different genotypes. Silicon application significantly decreased As concentrations in roots (p<0.005), straws (p<0.05) and husks (p<0.001) by 28-35%、15-35% and 32-57% respectively, whilst also reducing inorganic As (iAs) and DMA in rice grains. Indica genotypes with higher ROL accumulated lower concentrations of iAs in grains than hybrid genotypes with lower ROL.
Age-related macular degeneration (AMD) is a leading cause of irreversible central blindness among the elderly worldwide. We use exome sequencing to analyse nonsynonymous singlenucleotide variants (SNVs) across the whole genome of 216 neovascular AMD cases and 1,553 controls. As a follow-up validation, we evaluate 3,772 neovascular AMD cases and 6,942 controls from five independent cohorts in the East Asian population. Here we show strong evidence of an association at a novel, missense SNV,doi:10.1038/ncomms7687 pmid:25872646 fatcat:pjth6dlmqrgczjuyxzxwmbrmva
more »... 9323, which is located in the ubiquitin protein ligase E3D (UBE3D) gene (P meta ¼ 1.46 Â 10 À 9 , odds ratio (OR) ¼ 0.74, 95% confidence interval (CI): 0.63-0.88). Furthermore, ablation of the UBE3D protein lead to an abnormal amount of pigment granules deposited in retinal pigment epithelium microvilli area and an abnormal response on electroretinography (ERG) in UBE3D þ / À heterozygous mice. Our findings indicate that the ubiquitin-proteasome system may play a role in the pathogenesis of neovascular AMD.
Epithelial ovarian cancer (EOC) is one of the most malignant gynecological tumors worldwide. Deregulation of long non-coding RNAs (lncRNAs) has been implicated in various oncogenic processes in multiple cancers. In this study, we aim to identify and characterize clinically relevant lncRNA deregulation in EOC. Methods: LncRNAs, mRNAs and miRNAs were profiled using expression microarrays and validated using reverse transcription quantitative PCR in EOC cells and tissues. siRNAs targeting eitherdoi:10.1186/s13046-019-1103-5 fatcat:xlbyw2oox5bedaswopz2f6uxy4
more »... XD-AS1 or PIK3R3 together with miR-186-5p inhibitors were used to modulate endogenous target expression in EOC cell lines in vitro. In vitro wound healing assay, trans-well assay, Western-blot assay,and Dual-luciferase reporter assay were used to explore the biological roles and molecular function underlying HOXD-AS1 in the EOC cells. Progression-free survival (PFS) and overall survival (OS) were statistically analyzed by Kaplan-Meier method test. Results: HOXD-AS1 was found to be significantly over-expressed in EOC tumors. High HOXD-AS1 expression significantly correlated with poorer PFS and OS of EOC patients. Multivariate Cox proportional hazards modeling indicated that HOXD-AS1 was an independent risk predictor of EOC patients (HR = 1.92, p = 0.004). SiRNA inhibition of HOXD-AS1 reduced cell migration, invasion, and epithelial-mesenchymal transition (EMT) in EOC cells in vitro by preventing HOXD-AS1 directly binding to miR-186-5p, and resulting in down-regulating of PIK3R3. The novel HOXD-AS1/miR-186-5p/PIK3R3 pathway was clinically relevant as we observed a significantly inverse correlation between HOXD-AS1/miR-186-5p and between miR-186-5p/PIK3R3 in an independent cohort of 200 EOC tissues. Conclusions: HOXD-AS1/miR-186-5p/PIK3R3 is a novel pathway to promote cell migration, invasion, and EMT in EOC.
« Previous Showing results 1 — 15 out of 1,856 results