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Cortical neurons were grown from iPSCs using the methods described (Xu et al. 2016) . RNAs were isolated with the miRNeasy kit (Qiagen) according to the manufacturer's protocol. ...doi:10.1101/120394 fatcat:n3aq7lvn4bfepfjvdmirl3btbq
A novel fluorenone derivative, FO52, is designed and synthesized.doi:10.1039/d1ra01134k pmid:35481210 pmcid:PMC9031121 fatcat:t6cazbgesjgsbe6kj5enymmpqu
Cortical neurons were grown from iPSCs using the methods described (Xu et al. 2016) . RNAs were isolated with the miRNeasy kit (Qiagen) according to the manufacturer's protocol. ...doi:10.1101/gr.222067.117 pmid:28877962 pmcid:PMC5630040 fatcat:sp2weuqccndpnaigbzzusldf3u
Botch promotes embryonic neurogenesis by inhibiting the initial S1 furin-like cleavage step of Notch maturation. The biochemical process by which Botch inhibits Notch maturation is not known. Here, we show that Botch has γ-glutamyl cyclotransferase (GGCT) activity that deglycinates Notch, which prevents the S1 furin-like cleavage. Moreover, Notch is monoglycinated on the γ-glutamyl carbon of glutamate 1,669. The deglycinase activity of Botch is required for inhibition of Notch signaling both indoi:10.1016/j.celrep.2014.03.048 pmid:24767995 pmcid:PMC4031649 fatcat:627rsvygsvg3hjrzy2t4twgjby
more »... vitro and in vivo. When the γ-glutamyl-glycine at position 1,669 of Notch is degylcinated, it is replaced by 5-oxy-proline. These results reveal that Botch regulates Notch signaling through deglycination and identify a posttranslational modification of Notch that plays an important role in neurogenesis.
Human pluripotent stem cells (PSCs) are a promising cell resource for various applications in regenerative medicine. Highly efficient approaches that differentiate human PSCs into functional lineagespecific neurons are critical for modeling neurological disorders and testing potential therapies. Proneural transcription factors are crucial drivers of neuron development and hold promise for driving highly efficient neuronal conversion in PSCs. Here, we study the functions of proneuraldoi:10.5966/sctm.2013-0213 pmid:24904172 pmcid:PMC4116248 fatcat:mu6dmgljenad3ee4twsqqcvirm
more »... n factor Atoh1 in the neuronal differentiation of PSCs. We show that Atoh1 is induced during the neuronal conversion of PSCs and that ectopic Atoh1 expression is sufficient to drive PSCs into neurons with high efficiency. Atoh1 induction, in combination with cell extrinsic factors, differentiates PSCs into functional dopaminergic (DA) neurons with >80% purity. Atoh1-induced DA neurons recapitulate key biochemical and electrophysiological features of midbrain DA neurons, the degeneration of which is responsible for clinical symptoms in Parkinson's disease (PD). Atoh1-induced DA neurons provide a reliable disease model for studying PD pathogenesis, such as neurotoxin-induced neurodegeneration in PD. Overall, our results determine the role of Atoh1 in regulating neuronal differentiation and neuron subtype specification of human PSCs. Our Atoh1-mediated differentiation approach will enable large-scale applications of PD patient-derived midbrain DA neurons in mechanistic studies and drug screening for both familial and sporadic PD. STEM CELLS
Springer, New York, NY, 1996 edition, November 2013.  Haoyin Xu at al. When are deep networks really better than decision forests at small sample sizes, and how? ... Cowan1 ; Maxim Bichuch1 ; Archana Venkataraman1 ; Chen Li1 ; Ni- tish Thakor1 ; Justus M Kebschull1 ; Marilyn Albert1 ; Jinchong Xu1 ; Marshall Hussain Shuler1 ; Brian Caffo1 ; Tilak Ratnanather1 ; Ali ...arXiv:2201.07372v1 fatcat:6qktqmoffvd4zl37ubd4wo2f2m
Acknowledgements We are grateful to Jinchong Xu and Yifang Cui for insightful suggestions for experimental procedures, Achim Dahlmann for genotyping, and Andreas Faissner and Niels Danbolt for antibodies ...doi:10.1242/jcs.088120 pmid:22159417 fatcat:iy6txvcpnvgi7jgj6shmrsq7lq
He was fond of socialising in civil academic groups such as the Dunshe Society, Chengjiao Society, and Jinchong Shan Tang (Yang, 1981) . ... Xu Jiyu was one of the few who made a substantial breakthrough in the distinction between Cathay and barbarians. ...doi:10.25949/19428308 fatcat:v74ecoedprgznpfzmo336kzlzu