A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is
A broadband photonic analog-to-digital converter (ADC) for X-band radar applications is proposed and experimentally demonstrated. An X-band signal with arbitrary waveform and a bandwidth up to 2 GHz can be synchronously sampled and processed due to the optical sampling structure. In the experiment, the chirp signal centered at 9 GHz with a bandwidth of 1.6 GHz is sampled and down-converted with a signal-to-noise ratio of 7.20 dB and an improved noise figure. Adopting the photonic ADC in thedoi:10.3788/col201816.060605 fatcat:4i25gyztfrhzvbxdsnkigd3zhu
more »... r receiver and the above signal as the transmitted radar signal, an X-band inverse synthetic aperture radar system is set up, and the range and cross-range resolutions of 9.4 and 8.3 cm are obtained, respectively.
Over the centuries, scientists and engineers have been fascinated by the directional transport of water on the peristome of pitcher plant. Through experimental investigation and theoretical analysis, here we reveal the more complex picture of droplet transport on this peculiar natural surface. First, we demonstrate that in addition to the presence of the asymmetric arch-shaped microcavity with gradient wedge corners and sharp edges, the structural gradient in the first-tier microgroove of thedoi:10.1038/s42005-018-0038-z fatcat:5yx5spnxlnbf5nb5vnhgvr5eea
more »... tcher's peristome also plays an important role in the regulation of the directional droplet transport. Moreover, the directional liquid transport only occurs in a limited condition. Without the intricate control of the interplay between its multiscale structures and multiscale sources of water, as well as the dynamic conditions of water, the preferential directional droplet transport will collapse. The new transport phenomenon and the mechanisms we reveal will provide important insights for the design of asymmetric morphologies for droplet manipulation.
Multimodal sentiment analysis has currently identified its significance in a variety of domains. For the purpose of sentiment analysis, different aspects of distinguishing modalities, which correspond to one target, are processed and analyzed. In this work, we propose the targeted aspect-based multimodal sentiment analysis (TABMSA) for the first time. Furthermore, an attention capsule extraction and multi-head fusion network (EF-Net) on the task of TABMSA is devised. The multi-head attentionarXiv:2103.07659v1 fatcat:z2px4ll7ong7fpkdqride47lyy
more »... A) based network and the ResNet-152 are employed to deal with texts and images, respectively. The integration of MHA and capsule network aims to capture the interaction among the multimodal inputs. In addition to the targeted aspect, the information from the context and the image is also incorporated for sentiment delivered. We evaluate the proposed model on two manually annotated datasets. the experimental results demonstrate the effectiveness of our proposed model for this new task.
Shikonin, as a traditional Chinese herbal medicine with a role of anti-cancer, anti-inflammatory, anti-bacterial and other effects. However, there are few studies on the effect of shikonin on osteoporosis. Therefore, the purpose of this study aims to investigate the role and mechanism of shikonin on differentiation of BMSCs and BMMs into osteoblasts and osteoclasts formation. In our study, we treated the cells with different concentrations of shikonin, and then illuminated its effect onpmid:33437384 pmcid:PMC7791499 fatcat:nw6zminelna4zgy6xyo7jtrzuq
more »... sis and osteoclast differentiation by ALP/alizarin red staining, ALP activity, qRT-PCR, immunofluorescence, Western blot, and TRAP staining. The result showed that shikonin may promote BMSCs differentiate into osteoblasts through the Wnt/β-catenin signaling pathway. At the same time, it may also inhibit the formation of osteoclasts mediated by RANK/RANKL/OPG pathway in vitro. Our research explains excellently the mechanism of shikonin alleviating osteoporosis in vitro, which maybe contributing to the exploration of a new way to prevent osteoporosis.
The profiling of physiological response of cells to external stimuli at the single cell level is of importance. Traditional approaches to study cell responses are often limited by ensemble measurement, which is challenging to reveal the complex single cell behaviors under a dynamic environment. Here we report the development of a simple microfluidic device to investigate intracellular calcium response to dynamic hypertonic conditions at the single cell level in real-time. Interestingly, adoi:10.1038/srep23591 pmid:27004604 pmcid:PMC4804238 fatcat:tsqqyskpprexdjeee6r4xs4gde
more »... ic elevation in the intracellular calcium signaling is found in both suspension cells (human leukemic cell line, HL-60) and adherent cells (lung cancer cell line, A549), which is ascribed to the exposure of cells to the hydrodynamic stress. We also demonstrate that the calcium response exhibits distinct single cell heterogeneity as well as cell-type-dependent responses to the same stimuli. Our study opens up a new tool for tracking cellular activity at the single cell level in real time for high throughput drug screening. The study of cellular physiological responses to external microenvironments with high spatial and temporal resolution is of importance for probing cell signaling and function 1-5 . Exposed to different stimuli, various cellular responses will be activated such as the cell shrinkage (volume decrease) 6,7 , gene expression 8 and underlying ion movement (potassium, sodium and calcium) 9-11 . In particular, the calcium signaling of most of cells is mediated by various molecular pathways, e.g., inositol trisphosphate (IP3), adenosine 5′ -triphosphate (ATP), prostaglandin E2 (PGE2), and nitric oxide (NO). IP3 can lead to a rapid release of calcium stored in the ER via binding to the ER membrane receptor. After the cytosolic calcium concentration is elevated to a critical level by intra/extracellular sources, the depleted intracellular calcium stores tend to recover their calcium reservation to original level and become ready for the next release of calcium 12-14 . Traditional approaches to study the intracellular calcium response typically involve the exposure of a group of cells to external environments. Such ensemble measurement could not reflect the heterogeneity of individual cells in transient response subject to dynamically changing environments 15 . Different from ensemble measurements, single cell analysis is capable of revealing the complex, highly orchestrated physiology of individual cellular processes 16, 17 . Although patch clamp could be used to detect the cellular response to external conditions in real-time, the immobilization of single cells by patch clamp is rather labor-intensive and time-consuming, which is inherently not amenable for high-throughput analysis 18 . Flow cytometry could achieve high-throughput single cell detection, but it is an end-point analysis and the dynamic change in cellular activity could not be monitored in real-time 16, 19 . Microfluidic technologies offer advantages of high-throughput single-cell measurements of cellular responses 20-22 . One promising approach is to trap single cell in large microwell arrays, which have been demonstrated for drug screening, toxicology, and fundamental cell biology     . In this work, we report the monitoring time resolved intracellular calcium response to dynamic hypertonic conditions using a simple microwell-based microfluidic device. The hypertonic condition is regulated by the liquid evaporation which serves to mimic dynamic osmolality decrease that cells might encounter in vivo. Interestingly, a substantial elevation in the intracellular calcium signaling is found in both suspension cells (human leukemic HL-60 cells) and adherent cells (lung cancer A549 cells) at certain hypertonic condition, though the intracellular calcium response exhibits obvious cell-type-specific difference as well as pronounced
Purpose The purpose of this study was to provide cumulative data about the intermediate to long-term outcome of Scandinavian total ankle replacement (STAR) in the literature and to provide a summary of survival rate, implant failure rate and reasons. Methods A comprehensive search for all relevant articles published in English and German from January 1995 to May 2011 was conducted. Two reviewers evaluated each study to determine whether it was eligible for inclusion and, if so, collected datadoi:10.1007/s00264-011-1339-y pmid:21881886 pmcid:PMC3224628 fatcat:ffk56loqonemhd2hozb52fc5j4
more »... interest. The intermediate to longterm outcomes were determined. Evidence-based metaanalytic pooling of results across studies was performed to determine survival and failure rates. Results Sixteen primary studies with 2,088 implants were identified. The mean American Orthopaedic Foot and Ankle Society (AOFAS) score was 77.8 points, and the mean Kofoed ankle score was 76.4 points. The pooled mean five year survival rate was 85.9% [95% confidence interval (CI) 80.9-90.3], and the pooled mean ten year survival rate was 71.1% (95% CI 60.9-81.5). Pooled failure rate was 11.1% (95% CI 7.6 -14.9), with a mean follow-up time of 52 months; 41% failed within one year of initial operation. The first three reasons associated with implant failure were aseptic loosening (5.2%), malalignment (1.7%) and deep infection (1.0%). Conclusions We found that STAR prosthesis achieved encouraging results in terms of intermediate to long-term outcome. The five and ten year survival rates were acceptable. However, the failure rate was still high. The major reasons for implant failure were aseptic loosening and malalignment. Maybe the increase of surgeons' experience and patient selection could improve outcomes and decrease failure rate.
Yuling Tang, Jinliang Zhang, Xiubin Li, Chong Zhang, Yanxiao Wang and Jiaqian Xu performed the experiments and analysed data. Guan Yang and Yeguang Chen provided technical assistance. ... Acknowledgements Author contributions Yuling Tang, Yan Teng, and Xiao Yang conceived and coordinated the study and wrote the paper. ...doi:10.7150/ijbs.28879 fatcat:445mlfjzlrfaxdau26lpnbari4
and Aim: Biliary atresia (BA), an inflammatory destruction of the bile ducts, leads to liver fibrosis in infants and accounts for half of cases undergoing pediatric liver transplantation. Yes-associated protein (YAP), an effector of the Hippo signaling pathway, is critical in maintaining identities of bile ductal cells. Here, we evaluated the expression of YAP and YAP target genes in BA livers and a rhesus rotavirus (RRV)-induced BA mice model.Methods: Liver specimens collected from 200 BAdoi:10.3389/fped.2020.618226 pmid:33553074 pmcid:PMC7859521 fatcat:knkqzerijrhdpmf42jdc3hz334
more »... nts were compared with those of 30 non-BA patients. Model mice liver tissues were also collected. The expression of YAP and YAP target genes were measured by transfection, RNA-seq, immunohistochemistry, immunoblot, and quantitative PCR. Masson's trichrome staining and the Biliary Atresia Research Consortium (BARC) system were utilized to score liver fibrosis status.Results: The expression of YAP is elevated and positively correlated with fibrosis in BA livers. Moreover, ANKRD1, which is identified as the target gene of YAP, is also highly expressed in BA livers. Consistent with clinical data, YAP and ANKRD1 are significantly upregulated in RRV-induced BA mouse model.Conclusions: YAP expression is closely correlated with the bile duct hyperplasia and liver fibrosis, and may serve as an indicator for liver fibrosis and BA progression. This study indicates an involvement of the Hippo signaling pathway in the development of BA, and the YAP induced ANKRD1 expression may also be related to bile duct hyperplasia in BA. This provides a new direction for more in-depth exploration of the etiology and pathogenesis of biliary atresia.
RNA editing (DNA/RNA differences) as a post-transcriptional modification approach to enrich genetic information, plays the crucial role in regulating diverse biological processes in eukaryotes. Although it has been extensively studied in plant chloroplast and mitochondria genome, RNA editing in plant nuclear genome, especially those associated with Fusarium head blight (FHB), is not well studied at present. Here, we investigated the DNA/RNA differences associated with FHB through a novel methoddoi:10.1101/2021.06.25.449684 fatcat:34iofhqlhbgc5osghebppk7g2u
more »... by comparing the RNA-seq data from Fusarium-infected and control samples from 4 wheat genotypes. A total of 187 DNA/RNA differences were identified in 36 wheat genes, representing the first landscape of the FHB-responsive RNA editome in wheat. Furthermore, all of these 36 edited genes were located in the FHB related co-expression gene modules, which may involve in regulating FHB response. Finally, the effects of DNA/RNA differences were systematically investigated to show that they could cause the change of RNA structure and protein structure in edited genes. In particular, the G to C editing (chr3A_487854715) in TraesCS3A02G263900, which is the orthology of OsRACK1, resulted that it was targeted by tae-miR9664-3p to control its expression in different genotype through different editing efficiency, suggesting RNA editing could mediate miRNA to participate in the regulation network of FHB tolerance. This study reported the first wheat DNA/RNA differences associated with FHB, which not only contribute to better understand the molecular basis underlying FHB tolerance, but also shed light on improving FHB tolerance through epigenetic method in wheat and beyond.
To reduce the possibility of bacterial infection and implant-related complications, surface modification on polyurethane (PU) film is an ideal solution to endow hydrophobic PU with antibacterial and antifouling properties. In this work, a variety of polyhexamethylene guanidine/ hyaluronic acid (PHMG/HA) multilayer films were self-assembled layer-by-layer on PU films using polyanions, carboxyl-activated HA, and polycations PHMG by controlling the concentration of these polyelectrolytes as welldoi:10.3390/polym13060934 pmid:33803560 fatcat:tfuqe2yg4bcqfaharnmn2jp4gi
more »... the number of layers self-assembled. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) spectra, water contact angle (WCA), and A Atomic force microscope (AFM) of PU and modified PU films were studied. Protein adsorption and bacterial adhesion as well as the cytotoxicity against L929 of the film on selected PU-(PHMG/HA)5/5-5 were estimated. The results showed that PU-(PHMG/HA)5/5-5 had the best hydrophilicity among all the prepared films, possessing the lowest level of protein adsorption. Meanwhile, this film showed efficient broad-spectrum antibacterial performance as well as significant resistance of bacterial adhesion of more than a 99.9% drop for the selected bacteria. Moreover, almost no influence on cell viability of L929 enhanced the biocompatibility of film. Therefore, the modified PU films with admirable protein absorption resistance, antimicrobial performance, and biocompatibility would have promising applications in biomedical aspect.
Dissociation pressures of some gas hydrates have been evaluated using the Lennard-Jones 12-6 28-7 and Kihara potentials in the Lennard-Jones-Devonshire cell model. The Lennard-Jones 28-7 pot~ntial 'gives the least satisfactory results. The Lennard-Jones 12-6 potential works satisfactorily for the monatomic gases and ClL but poorly for the rodlike molecules C2H6, C02, N2, 02, C2fu. This failure may be due to (i) d~~.tortio~s of t~e hydrate la~tice, (ii) neglect of.m?lecul~r shape and size indoi:10.1063/1.3469780 pmid:20815623 fatcat:c6tb467ud5galdaqsxhhovxjje
more »... rmining the cavity potential (m) bamer to mternal rotatIOn of the molecule m Its cavity. A crude model for the lattice shows that it is not distorted. The Kihara potential predicts better dissociation pressures for the hydrates of the rodlike moleules. U,nlike the previously used Lennard-Jones 12-6 potential, it depends on the size and shape of the mteractmg molecules. The absence of lattice distortions, improved dissociation pressures through the use of the Kihara potential and the restriction of the motion of the solute molecule to around the center of a cavity makes a large barrier to rotation unlikely. A small barrier may still be present.
Lung cancer is the leading cause of cancer related deaths worldwide. TGF-β-induced epithelial-mesenchymal transition (EMT) is a key cell-intrinsic identity for tumor cell migration, invasion, and stemness acquisition in cancer metastasis. Long noncoding RNAs (lncRNAs) have not been fully investigated for their involvement in regulating TGF-β-induced EMT and metastasis in lung cancer. Here, we demonstrated that the transcription of lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1) wasdoi:10.7150/ijbs.27197 pmid:30416386 pmcid:PMC6216035 fatcat:7772lpanovgwhet6pg7dr7fwxy
more »... inhibited by TGF-β1 in a SMAD4-dependent manner. LINP1 suppressed EMT of lung cancer cells, thereby controlling cancer cell migration, invasion, and stemless. Moreover, LINP1 inhibited TGF-β-induced EMT and cell invasion in lung cancer cells. Our study reveals the role of LINP1 in the regulation of TGF-β-induced EMT in human lung cancer.
Qiang Yang is currently a Chair Professor in the Department of Computer Science and Engineering of the Hong Kong University of Science and Technology. ... has • has Jiaqian Ren is with Institute of Information Engineering, Chinese Academy of Sciences, and the School of Cyber Security, University of Chinese Academy of Sciences, Beijing 100041, China. ... Jiaqian Ren is currently a Ph.D. candidate in Institute of Information Engineering, Chinese Academy of Sciences. ...arXiv:2108.03084v2 fatcat:kiq6t3w7qnfxxa4bvitt6gdpse
Li J, Tseng K-K, Hsieh ZY, Yang CW, Huang H-N (2020) Correction: Staining Pattern Classification of Antinuclear Autoantibodies Based on Block Segmentation in Indirect Immunofluorescence Images. ...doi:10.1371/journal.pone.0236463 pmid:32726332 fatcat:hl6jgxcflbgmzlzgmhcvlbpk6e
Date of publication xxxx 00, 0000, date of current version xxxx 00, 0000.doi:10.1109/access.2021.3126782 fatcat:z2utgiyl55hixgon2oe4pxubf4
« Previous Showing results 1 — 15 out of 91 results