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Form and Function in Protein Dephosphorylation

John M Denu, Jeanne A Stuckey, Mark A Saper, Jack E Dixon
1996 Cell  
A sub-family of ranching enzymes.  ...  These structures revealed a common Oregon Health Sciences University Medical School motif of a central ␤-␣-␤-␣-␤ scaffold containing a di-Department of Biochemistry and Molecular Biology L224 nuclear metal  ...  It is attractive to (Stuckey et al., 1994) .  ... 
doi:10.1016/s0092-8674(00)81356-2 pmid:8898189 fatcat:2pwajimep5gpjnav66etkzunk4

Hydrolytic catalysis and structural stabilization in a designed metalloprotein

Melissa L. Zastrow, Anna F. A. Peacock, Jeanne A. Stuckey, Vincent L. Pecoraro
2011 Nature Chemistry  
Here, we present an artificial metallohydrolase, which has been shown by X-ray crystallography to contain two different metal ions -a Zn(II) ion which is important for catalytic activity and a Hg(II) ion  ...  Reproducing these functions in a designed protein is the ultimate challenge to our understanding of them.  ...  a .  ... 
doi:10.1038/nchem.1201 pmid:22270627 pmcid:PMC3270697 fatcat:uelxhy2spnc4bkyknvevfboule

A ligand-induced conformational change in theyersiniaprotein tyrosine phosphatase

Heidi L. Schubert, Eric B. Fauman, Jeanne A. Stuckey, Jack E. Dixon, Mark A. Saper
1995 Protein Science  
The structures of several different PTPases have revealed a conserved active site architecture in which a phosphate-binding loop, together with an invariant arginine, cradle the phosphate of a phosphotyrosine  ...  We previously reported that binding of tungstate to the Yop5 l PTPase from Yersiniu induced a loop conformational change that moved aspartic acid 356 into the active site, where it can function as a general  ...  oxygen of a phosphotyrosine substrate (Stuckey et al., 1994) .  ... 
doi:10.1002/pro.5560040924 pmid:8528087 pmcid:PMC2143214 fatcat:crbc53tly5e2lmnripm4sbn5lq

The Tail of KdsC

Tapan Biswas, Li Yi, Parag Aggarwal, Jing Wu, John R. Rubin, Jeanne A. Stuckey, Ronald W. Woodard, Oleg V. Tsodikov
2009 Journal of Biological Chemistry  
B, a diagram of the specific interactions of Kdo and P i in the KdsC active site. Interatomic distances shorter than 4 Å are shown by dashed lines.  ...  Here, we present a set of seven structures of tetrameric Escherichia coli KdsC (ranging from 1.4 to 3.06 Å in resolution) that model different intermediate states in its catalytic mechanism.  ...  The backbones of the loops undergo a movement of several Å, e.g. the C␣ atom of Arg-78 of loop 1 moves by 3.01 Å, and the C␣ atom of Ser-100 of loop 2 moves by 4.13 Å.  ... 
doi:10.1074/jbc.m109.012278 pmid:19726684 pmcid:PMC2781614 fatcat:t2wyzqag7vai7jbga7ykwlq5oi

A Unique Carbohydrate Binding Domain Targets the Lafora Disease Phosphatase to Glycogen

Jianyong Wang, Jeanne A. Stuckey, Matthew J. Wishart, Jack E. Dixon
2001 Journal of Biological Chemistry  
EPM2A encodes a 331-amino acid protein containing a carboxyl-terminal phosphatase catalytic domain.  ...  (Novagen) to produce a recombinant protein with a COOH-terminal 6-histidine tag.  ...  A, COS1 cells were transiently co-transfected with pEGFP-Laf and pcDNA4/Myc-GS.  ... 
doi:10.1074/jbc.c100686200 pmid:11739371 fatcat:istsp7ouqrb53lyyuoe2tkul2e

Switching the Chirality of the Metal Environment Alters the Coordination Mode in Designed Peptides

Anna F. A. Peacock, Jeanne A. Stuckey, Vincent L. Pecoraro
2009 Angewandte Chemie  
) 3 À  ...  to a three-coordinate site  ... 
doi:10.1002/ange.200902166 fatcat:e4aqzklborgx7jnx5ezikwivxq

LplA1-dependent utilization of host lipoyl peptides enables Listeria cytosolic growth and virulence

Kristie M. Keeney, Jeanne A. Stuckey, Mary X. D. O'Riordan
2007 Molecular Microbiology  
Thus, the ability of L. monocytogenes and other intracellular pathogens to efficiently use host lipoyl peptides as a source of lipoate may be a requisite adaptation for life within the mammalian cell.  ...  L. monocytogenes is a lipoate auxotroph and must scavenge this critical cofactor, using lipoate ligases to facilitate attachment of the lipoyl moiety to metabolic enzyme complexes.  ...  Stuckey and M. X. D. O'Riordan describes how LplA1 contributes to L. monocytogenes pathogenesis. Table 1 . 1 Strains used.  ... 
doi:10.1111/j.1365-2958.2007.05956.x pmid:17908209 pmcid:PMC2367003 fatcat:kgas5yiktnb25bmvundawsywci

A Crystallographic Examination of Predisposition versus Preorganization in de Novo Designed Metalloproteins

Leela Ruckthong, Melissa L. Zastrow, Jeanne A. Stuckey, Vincent L. Pecoraro
2016 Journal of the American Chemical Society  
the orientations of Cys side chains in the 16th layer of apo-(CSL16C) 3 ; Figure S3 , the similarity of the major Cys conformer orientation in apo-(CSL16C) 3 and apo-(CSL9C) 3 structures; Figure S4 , a  ...  high level of preorganization of Pen ligands toward a trigonal planar Hg(II)-binding site in 3SCC peptides; Figure S5 , an alignment of the helical backbones between Hg(II) S Zn-(II) N (GRAND-CSL16CL30H  ...  This is because the interlayer spacing on going from a to d residues is smaller (~4.41 Å) than on progressing from d to a in the sequence (~4.92 Å).  ... 
doi:10.1021/jacs.6b07165 pmid:27532255 pmcid:PMC5242185 fatcat:n5dqieexa5cyrpixxpvnv2v3wu

Crystal Structure of Patatin-17 in Complex with Aged and Non-Aged Organophosphorus Compounds

Sanjeeva J. Wijeyesakere, Rudy J. Richardson, Jeanne A. Stuckey, Israel Silman
2014 PLoS ONE  
Patatin is a non-specific plant lipase and the eponymous member of a broad class of serine hydrolases termed the patatinlike phospholipase domain containing proteins (PNPLAs).  ...  Citation: Wijeyesakere SJ, Richardson RJ, Stuckey JA (2014) Crystal Structure of Patatin-17 in Complex with Aged and Non-Aged Organophosphorus Compounds. PLoS ONE 9(9): e108245.  ...  a/b hydrolase fold containing a central 6stranded b-sheet sandwiched by 9 a-helices along with a catalytic center composed of a Ser77-Asp215 dyad [6] .  ... 
doi:10.1371/journal.pone.0108245 pmid:25248161 pmcid:PMC4172759 fatcat:u2chvabggjfs7f5nhky7w66djq

Structural Basis for the Recognition of Peptide RJPXD33 by Acyltransferases in Lipid A Biosynthesis

Ronald J. Jenkins, Kyle A. Heslip, Jennifer L. Meagher, Jeanne A. Stuckey, Garry D. Dotson
2014 Journal of Biological Chemistry  
To gain a fundamental understanding of the molecular basis of inhibitor binding, we determined the crystal structure of LpxA from Escherichia coli in complex with RJPXD33 at 1.9 Å resolutions.  ...  Overlay of the LpxA-RJPXD33 structure with E. coli LpxD identified a complementary peptide binding pocket within LpxD and serves as a model for further biochemical characterization of RJPXD33 binding to  ...  min at a distance of ϳ2 cm.  ... 
doi:10.1074/jbc.m114.564278 pmid:24742680 pmcid:PMC4140908 fatcat:gnjfxuo5mfaatjqv54bnwrcb5m

Switching the Chirality of the Metal Environment Alters the Coordination Mode in Designed Peptides

Anna F. A. Peacock, Jeanne A. Stuckey, Vincent L. Pecoraro
2009 Angewandte Chemie International Edition  
) 3 À  ...  to a three-coordinate site  ... 
doi:10.1002/anie.200902166 pmid:19579245 pmcid:PMC3014729 fatcat:4rfk3kxjd5do3hu5dq4zcjgu7a

A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor

Angelo Aguilar, Haibin Zhou, Jianfang Chen, Liu Liu, Longchuan Bai, Donna McEachern, Chao-Yie Yang, Jennifer Meagher, Jeanne Stuckey, Shaomeng Wang
2013 Journal of Medicinal Chemistry  
Compound 32 is capable of achieving rapid, complete and durable tumor regression in vivo at a well-tolerated dose-schedule.  ...  a Dean-Stark apparatus.  ...  Chemistry Initially, we employed a convergent synthesis 7 (Method A) for the preparation of the target molecules.  ... 
doi:10.1021/jm4001105 pmid:23448298 pmcid:PMC3806060 fatcat:tu3f2hn7vrfc5ndlwcl5bg3n54

The X-ray Crystal Structures ofYersiniaTyrosine Phosphatase with Bound Tungstate and Nitrate

Eric B. Fauman, Chantana Yuvaniyama, Heidi L. Schubert, Jeanne A. Stuckey, Mark A. Saper
1996 Journal of Biological Chemistry  
These energy-minimized models are consistent with a general acid-catalyzed, in-line displacement of the phosphate moiety to Cys 403 on the enzyme, followed by attack by a nucleophilic water molecule to  ...  Both an associative (bond formation preceding bond cleavage) and a dissociative (bond cleavage preceding bond formation) mechanism were modeled, but a dissociative-like mechanism is favored for steric  ...  Fig. 2 was made with O and VORT, with a surface generated by GRASP (47) . Figs. 3 and 4 were made using MOLSCRIPT (48) and VORT.  ... 
doi:10.1074/jbc.271.31.18780 pmid:8702535 fatcat:4kwhotebwvejpiiikmklfal4v4

Expression, Characterization, and Mutagenesis of theYersinia pestisMurine Toxin, a Phospholipase D Superfamily Member

Amy E. Rudolph, Jeanne A. Stuckey, Yi Zhao, Harry R. Matthews, Walter A. Patton, Joel Moss, Jack E. Dixon
1999 Journal of Biological Chemistry  
The role of the conserved residues in catalysis was further defined by the isolation of a radiolabeled phosphoenzyme intermediate, which identified a conserved histidine residue as the nucleophile in the  ...  A phospholipase D (PLD) superfamily was recently identified that contains proteins of highly diverse functions with the conserved motif HXKX 4 DX 6 G(G/S).  ...  A. Orth and C. A. Worby for critical reading of the manuscript, E. B. Gottlin for helpful discussions, and J. Zhou and K. Chan for excellent technical assistance.  ... 
doi:10.1074/jbc.274.17.11824 pmid:10207000 fatcat:sd43dbuvlvczbcvt374iqwuwgy

d -Cysteine Ligands Control Metal Geometries within De Novo Designed Three-Stranded Coiled Coils

Leela Ruckthong, Anna F. A. Peacock, Cherilyn E. Pascoe, Lars Hemmingsen, Jeanne A. Stuckey, Vincent L. Pecoraro
2017 Chemistry - A European Journal  
We examine the effect of a D-configuration cysteine within a designed L-amino acid three-stranded coiled coil in order to enforce a precise coordination number on a metal center.  ...  Thus, the use of non-coded amino acids in a metal's coordination sphere promises to be a powerful tool for controlling the properties of future metalloproteins.  ...  The fourth ligand is a chloride ion at a distance of 2.43 Å, giving an average Zn(II)-ligand distance of 2.2(2) Å, which is reasonable for such a binding mode.  ... 
doi:10.1002/chem.201700660 pmid:28384393 pmcid:PMC5518473 fatcat:rcy2onajqvffnfvzb35gaiox5i
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