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Mouse cell line authentication
2013
Cytotechnology (Dordrecht)
Preserved Fulltext
Vero cells) (Hill et al. 2009; Almeida et al. 2011 ). ...
R: GTTTCTTCATGTGGCTGGTATGCTGTT
0.075
9-2
NT_039474.8
74395400-74395000
F: [VIC]-GGATTGCCAAGAATTTGAGG
R: GTTTCTTTCCTGAGTTGTGGACAGGGTTA
0.080
15-3
NT_039617.8
4930200-4930500
F: [NED]- ...
doi:10.1007/s10616-013-9545-7
pmid:23430347
pmcid:PMC3886540
fatcat:wq26y3idyjdqlcmnyf3ztyyzyq
Memoir: template-based structure prediction for membrane proteins
2013
Nucleic Acids Research
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Membrane proteins are estimated to be the targets of 50% of drugs that are currently in development, yet we have few membrane protein crystal structures. As a result, for a membrane protein of interest, the much-needed structural information usually comes from a homology model. Current homology modelling software is optimized for globular proteins, and ignores the constraints that the membrane is known to place on protein structure. Our Memoir server produces homology models using alignment and
doi:10.1093/nar/gkt331
pmid:23640332
pmcid:PMC3692111
fatcat:az47ghyxfrfjbo4s2qz4inizji
more »
... coordinate generation software that has been designed specifically for transmembrane proteins. Memoir is easy to use, with the only inputs being a structural template and the sequence that is to be modelled. We provide a video tutorial and a guide to assessing model quality. Supporting data aid manual refinement of the models. These data include a set of alternative conformations for each modelled loop, and a multiple sequence alignment that incorporates the query and template. Memoir works with both a-helical and b-barrel types of membrane proteins and is freely available at
MP-T: improving membrane protein alignment for structure prediction
2012
Computer applications in the biosciences : CABIOS
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In previous work, we performed pairwise alignment of a sequence on to a template (Hill et al., 2011) . ...
In previous work, we demonstrated that environment-specific substitution tables improved the pairwise alignment of membrane proteins (Hill et al., 2011) . ...
doi:10.1093/bioinformatics/bts640
pmid:23110969
fatcat:sv3kgc6yy5hy7ectfc33okg324
Authentication of African green monkey cell lines using human short tandem repeat markers
2011
BMC Biotechnology
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R -GATTGAACCAGATGGGAACGA
354-374
59
D17S1304 F -ACCATGTCCTCTGGTTCTGG
R -
GTTTCTTACAGGTGGGACTTGGTGAAA
197-213
60
D4S2408 F -TCATTTCCATAGGGTAAGTGAAAA
R -GCCATGGGGATAAAATCAGA
336-360
60 ...
*
267-291
55
DYS389
F -CCAACTCTCATCTGTATTATCTATG
R -GTCTTATCTCCACCCACCAGA
321-337
60
D1S518
F -GCAGATCTTGGGACTTCTCG
R -GTGTGGGCAACTGCATTAGAG
182-198
55
D6S1017 F -CTGGCACAGGATAGGTGCTC ...
doi:10.1186/1472-6750-11-102
pmid:22059503
pmcid:PMC3221628
fatcat:hsnaayvvfjhplj5umnddgdhyha
Sinusoidal Voltage Protocols For Rapid Characterisation Of Ion Channel Kinetics
[article]
2017
bioRxiv
pre-print
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We chose 217 to use 0.3 µM because the dofetilide hERG IC 50 value is <10 nM which, assuming a Hill coefficient 218 of one, should correspond to >97% conductance block of I Kr at 0.3 µM dofetilide. ...
similar conditions/species.
187
Leak Corrections
188 We used the leak-step from −80 mV to −120 mV in order to leak-correct the experimental data, 189 according to: 190 I corrected = I raw − V /R ...
doi:10.1101/100677
fatcat:acifpx43gjdh5minsxhxzacaxu
First-generation shaped gel reactors based on photo-patterned hybrid hydrogels
2020
Reaction Chemistry & Engineering
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This paper reports the development of first-generation photo-patterned ring-shaped gel reactors that catalyse the hydrolysis of para-nitrophenol phosphate using a phosphatase enzyme.
doi:10.1039/d0re00109k
fatcat:kvmp7nznc5azlb7heaqzppwby4
Hemoglobin Control of Cell Survival/Death Decision Regulates in Vitro Plant Embryogenesis
2014
Plant Physiology
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Expression of AtKOD was detected by quantitative RT-PCR, Taqman technology using AtKOD-F/R with AtKOD-FAM probe, and Actin1-F/R with Actin1-HEX probe (Supplemental Table S1 ). ...
In plants, Hbs are regulated by many abiotic and biotic stress responses characterized by the manifestation of PCD (Hill, 2012) . ...
doi:10.1104/pp.114.239335
pmid:24784758
pmcid:PMC4044835
fatcat:rfcxtqh2jrfopax4ctyzzydic4
Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics
2018
Journal of Physiology
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Key points r Ion current kinetics are commonly represented by current-voltage relationships, time constant-voltage relationships and subsequently mathematical models fitted to these. ...
are affected by changing conditions. r The new model predicts the current under traditional square-wave protocols well, with better predictions of underlying currents than literature models. ...
Leak corrections We used the leak step from −80 to −120 mV in order to leak-correct the experimental data, according to: I corrected = I raw − V R leak . (2) We identified the most appropriate R leak value ...
doi:10.1113/jp275733
pmid:29573276
pmcid:PMC5978315
fatcat:3gns5ekpuvd4rfoke2my5uloxe
Corrosion Characteristics of Laser-Engineered Net Shaping Additively-Manufactured 316L Stainless Steel
2018
JOM: The Member Journal of TMS
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Characterization of Aluminum-Neutralized Sulfonated Styrenic Pentablock Copolymer Films
2012
Industrial & Engineering Chemistry Research
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Free volume information was obtained from the o-Ps lifetime data using the Tao-Eldrup model to relate the o-Ps lifetime to the average radius, r, of a free volume element 65 where Δr is the empirical ...
. = ̅ Δ − Δπ P n lRT V p [ ] w w w (6) where R is the gas constant, V̅ w is the molar volume of water (18 cm 3 mol −1 ), 61 and Δπ is the osmotic pressure difference, which was zero for pure water permeability ...
doi:10.1021/ie202546z
fatcat:yl5tqiisi5abthsnuqsqxszrjq
Inactivation of RASA1 promotes melanoma tumorigenesis via R-Ras activation
2016
OncoTarget
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RAS viral (r-ras) oncogene homolog) isoform. ...
Moreover, R-Ras increased and RASA1 suppressed Ral-A activation among Ras downstream effectors. ...
We also have shown that RASA1 functions as a RasGAP for the R-Ras isoform. ...
doi:10.18632/oncotarget.8127
pmid:26993606
pmcid:PMC5029671
fatcat:xzgilr6e75dd5b6tl3epdpnc7y
sj-pdf-1-pul-10.1177_20458940211025240 - Supplemental material for Vascular cell-specific roles of mineralocorticoid receptors in pulmonary hypertension
2021
Figshare
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Penumatsa, Rod R. Warburton, Deniz Toksoz, Jamie Wilson, Nicholas S. Hill, Iris Z. Jaffe and Ioana R. Preston: On behalf of THALES Registry Investigators in Pulmonary Circulation ...
doi:10.25384/sage.14810912.v1
fatcat:wfhr5vge2bcahpdaaog7fiyyiq
Increased Islet Cell Proliferation, Decreased Apoptosis, and Greater Vascularization Leading to β-Cell Hyperplasia in Mutant Mice Lacking Insulin
2002
Endocrinology
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R. ...
doi:10.1210/endo.143.4.8753
pmid:11897712
fatcat:bzb7m3546ncvzjn3ftbfuk7h5y
Strategies and challenges associated with recruiting retirement village communities and residents into a group exercise intervention
2018
BMC Medical Research Methodology
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Randomized controlled trials (RCTs) provide the highest level of scientific evidence, but successful participant recruitment is critical to ensure the external and internal validity of results. This study describes the strategies associated with recruiting older adults at increased falls risk residing in retirement villages into an 18-month cluster RCT designed to evaluate the effects of a dual-task exercise program on falls and physical and cognitive function.
doi:10.1186/s12874-018-0633-4
pmid:30572835
pmcid:PMC6302307
fatcat:floysif3irbrzhv2gp4s4xd72u
BronchUK: Protocol for an observational cohort study and biobank in bronchiectasis
2021
ERJ Open Research
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Hill has nothing to disclose. J.S. ...
R. Wilson has nothing to disclose. M.R. ...
doi:10.1183/23120541.00775-2020
pmid:33898620
pmcid:PMC8053912
fatcat:jakejxi4qbcmfa4lmh4c5c3roi
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