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Adapting the Surgical Apgar Score for Perioperative Outcome Prediction in Liver Transplantation
2017
Transplantation Direct
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The surgical Apgar score (SAS) is a 10-point scale using the lowest heart rate, lowest mean arterial pressure, and estimated blood loss (EBL) during surgery to predict postoperative outcomes. ...
An Apgar score for surgery. ...
The surgical Apgar score (SAS) is a 10-point scale first described in 2007 by Gawande et al, 12 as a simple means of grading patients after general or vascular operations using 3 intraoperative variables ...
doi:10.1097/txd.0000000000000739
pmid:29184910
pmcid:PMC5682766
fatcat:fzmnl2penfcmtn5eitqfdoe2gu
Predicting helix orientation for coiled-coil dimers
2008
Proteins: Structure, Function, and Bioinformatics
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Model ELEC assigns all occurrences of g-e' (parallel) or g-g' + e-e' (antiparallel) E-R, R-E, K-E or E-K pairs a weight of -1, while E-E, R-R, R-K, K-R, K-K, D-E, E-D and D-D pairs are given a weight of ...
The parallel set contained 120 structures with R 0 values of 4. Figure S4 ). ...
doi:10.1002/prot.22118
pmid:18506779
pmcid:PMC2562949
fatcat:hnxrwrsmavdwzlh6q4dz3hasg4
AB-Bind: Antibody binding mutational database for computational affinity predictions
2015
Protein Science
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Numerical correlations between computed and observed DDG values were low (r 5 0.16-0.45), but the potentials exhibited predictive power for classifying variants as improved vs weakened binders. ...
For each crystal structure, quality metrics such as the resolution, R work , R free , and MolProbity scores, and experimental conditions such as pH and temperature were included in the database. ...
Pearson r values were 0.52, 0.27, 20.33, and 0.23 for these energy terms, respectively, compared to 0.34 for the complete FoldX scoring function. ...
doi:10.1002/pro.2829
pmid:26473627
pmcid:PMC4815335
fatcat:ebpb2plozfa4fnb2cwfjmaixca
Gsk3 is a metabolic checkpoint regulator in B cells
2017
Nature Immunology
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P.R-R. was supported by a fellowship from the Cancer Centers Council (C3). The Animal Resources and Cancer Metabolism Cores at SBP are supported by NCI award number 5P30CA030199. ...
The following stimulations or inhibitors were used as indicated: 10 ng/ml rmBAFF (R&D Systems), 10 ng/ml recombinant protein IL-4 (eBioscience), 5 µg/ml anti-CD40 (eBioscience), 12 µg/ml anti-IgM (Jackson ...
doi:10.1038/ni.3664
pmid:28114292
pmcid:PMC5310963
fatcat:7pnpmh3xdfbo3i3ble77dbkxaq
Modeling Backbone Flexibility to Achieve Sequence Diversity: The Design of Novel α-Helical Ligands for Bcl-xL
2007
Journal of Molecular Biology
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ΔE total = E R−H − (E RC + E R ) (5) The energy of the isolated receptor is the same for all design calculations and can be ignored. ...
) to an isolated receptor (R) and a random coil (RC). ...
doi:10.1016/j.jmb.2007.04.069
pmid:17597151
pmcid:PMC1994813
fatcat:6tx7hgy3qff27mg4tscjrrjxbi
A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches
2012
PLoS ONE
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Citation: Apgar J, Ross M, Zuo X, Dohle S, Sturtevant D, et al. (2012) A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches. PLoS ONE 7(5): e37355. ...
This is not the case for our dataset of native exteins (R 2 = 0.0154). The distance to the active site may be functionally important to the intein. ...
doi:10.1371/journal.pone.0037355
pmid:22649521
pmcid:PMC3359363
fatcat:nec6ve4sqzbjhgbqg35pu5nm3u
Beyond CDR-grafting: Structure-guided humanization of framework and CDR regions of an anti-myostatin antibody
2016
mAbs
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R free is equivalent to R work , except that it is calcu-
lated for a randomly chosen 5% of reflections omitted from the refinement pro-
cess. ...
0.008
Bond angles ( )
1.08
1.18
Ã
R work D SjjF obs j -jF calc j/ SjF obs j. ...
doi:10.1080/19420862.2016.1215786
pmid:27625211
pmcid:PMC5058614
fatcat:fker6dparrcoponl7l3gfnmhui
Modeling and mitigation of high-concentration antibody viscosity through structure-based computer-aided protein design
2020
PLoS ONE
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Author Contributions Conceptualization: James R. Apgar, Gregory J. Carven, Eric M. Bennett, Laura Lin. ...
is similar to the predictive performance of the Sharma Score (R = 0.72) and the Tomar Score (R = 0.70) (Fig 7) . ...
The fit to the curve is determined by calculating the correlation coefficient (R 2 ). ...
doi:10.1371/journal.pone.0232713
pmid:32379792
fatcat:ekr7hzmasneutpr4wnvedk7tqi
Augmented Binary Substitution: Single-pass CDR germ-lining and stabilization of therapeutic antibodies
2015
Proceedings of the National Academy of Sciences of the United States of America
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Although humanized antibodies have been highly successful in the clinic, all current humanization techniques have potential limitations, such as: reliance on rodent hosts, immunogenicity due to high non-germ-line amino acid content, v-domain destabilization, expression and formulation issues. This study presents a technology that generates stable, soluble, ultrahumanized antibodies via single-step complementarity-determining region (CDR) germ-lining. For three antibodies from three separate key
doi:10.1073/pnas.1510944112
pmid:26621728
pmcid:PMC4687607
fatcat:pyqsvl45krf7dfb4wwanbkcmtq
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... immune host species, binary substitution CDR cassettes were inserted into preferred human frameworks to form libraries in which only the parental or human germ-line destination residue was encoded at each position. The CDR-H3 in each case was also augmented with 1 ± 1 random substitution per clone. Each library was then screened for clones with restored antigen binding capacity. Lead ultrahumanized clones demonstrated high stability, with affinity and specificity equivalent to, or better than, the parental IgG. Critically, this was mainly achieved on germ-line frameworks by simultaneously subtracting up to 19 redundant non-germ-line residues in the CDRs. This process significantly lowered non-germ-line sequence content, minimized immunogenicity risk in the final molecules and provided a heat map for the essential non-germ-line CDR residue content of each antibody. The ABS technology therefore fully optimizes the clinical potential of antibodies from rodents and alternative immune hosts, rendering them indistinguishable from fully human in a simple, single-pass process. antibody | paratope | plasticity | humanization | immunogenicity
Pharmacological AMPK activation induces transcriptional responses congruent to exercise in skeletal and cardiac muscle, adipose tissues and liver
2019
PLoS ONE
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Acknowledgments We thank John R. Thompson, Jennifer Cho, Hans Weber, Laura Lubbers, and Jianchao Yao for their technical and/or intellectual support during the execution of these studies. ...
doi:10.1371/journal.pone.0211568
pmid:30811418
pmcid:PMC6392219
fatcat:pgucncl2ibembetz7ueot57teu
Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease
2020
Journal of Biological Chemistry
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Serum free media include RPMI, 0.1% BSA and 7µg/ml puromycin. 100µg/mL of commercially available FGF23 (Cat# 2604-FG, R&D systems) and purified sKlotho variants were co-treated for 1 hour. ...
Serum levels of either HEK-or CHO-sKlotho were determined using an LC-MSMS method following immunoprecipitation of the protein using a monoclonal anti-human Klotho antibody MAB5334 obtained from R&D Systems ...
doi:10.1074/jbc.ra119.012144
pmid:32005658
pmcid:PMC7062171
fatcat:jwnt3o2dcvgwxceuhu3yplpdku
Page 176 of Revue Francaise de Gynecologie et d'Obstetrique Vol. 75, Issue 3
[page]
1980
Revue Francaise de Gynecologie et d'Obstetrique
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< 7 in der ersten Mi- nute) bei welchen eine rasche Besserung (Apgar-Score > 7 in der 5. ...
Fenner A., Busse H.G., Junce M., Mutter R. — Acid- base parameters and alveolar arterial oxygen tension gra- dients in healthy neonatal and _post-neonatal Europ. J. Pediat., 1976, 122, 69.
. ...
Virginia Apgar (1909–1974): Apgar score innovator
2018
Singapore medical journal
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M U S I C I A N E X T R A O R D I N A I R E Apgar never married and was an avid fisherman, philatelist and musician, performing in several orchestras on the cello and violin. ...
With the help of two colleagues, Dr Duncan Holaday and Dr Stanley James, Apgar learned new methods of testing blood gases and levels of anaesthesia in the blood of newborns. ...
doi:10.11622/smedj.2018091
pmid:30109352
pmcid:PMC6056375
fatcat:3dhev7w24rabppfvffndo5qa2u
Page 560 of Journal of the American Medical Women's Association Vol. 29, Issue 12
[page]
1974
Journal of the American Medical Women's Association
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.: Acronym of Apgar score, JAMA, 181: 353, 1962.
Apgar, V., and James, L. S.: Further observations on the newborn scoring system, Am J Dis Child, 104: 419, 1962.
Apgar, V., and Girdany, B. ...
., and Turpin, R.: Les chromo- somes humains en cultures, CR Acad Sci (Paris), 248: 602, 1959.
In the News
Lottie C. ...
Page 964 of Clinical Obstetrics and Gynecology Vol. 3, Issue 4
[page]
1960
Clinical Obstetrics and Gynecology
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The Internet Archive has digitized a microfilm copy of this work. It may be possible to borrow a copy for reading.
Apgar, V. A proposal for a new method of evaluation of the newborn
infant. Anesth. & Analg. 32:260, 1953.
ApGar, V., Houapay, D. A., James, L. S., Prince, C. E., Wetssror, I. M..
and Weiss, I. ...
A., James, L. S., Werssrot, I. M., and Berrien, C. Evaluation of the newborn infant—Second report. J.A.M.A. 168 :1985, 1958.
4. Brown, R. J. K. ...
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