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Image reconstruction of multiphoton microscopy data
2009
2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro
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The techniques introduced in this paper allow for accurate multi-scale image reconstruction of multi-photon microscopy data. ...
Multiphoton microscopy (MPM) is a non-invasive imaging modality that can provide high resolution images of fluorescence with low toxicity, high signal to noise ratio and optical sectioning deeper than ...
We have also started to apply these methods on biological multiphoton data. Our study examined data gathered from a histology slide at various collection times. ...
doi:10.1109/isbi.2009.5193171
pmid:22158826
pmcid:PMC3235336
dblp:conf/isbi/DootENW09
fatcat:aangx4ldfjfkxfxd3thiz2rxay
Quantitative Analysis of Monocyte Subpopulations in Murine Atherosclerotic Plaques by Multiphoton Microscopy
2012
PLoS ONE
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Citation: Haka AS, Potteaux S, Fraser H, Randolph GJ, Maxfield FR (2012) Quantitative Analysis of Monocyte Subpopulations in Murine Atherosclerotic Plaques by Multiphoton Microscopy. ...
Next, we demonstrate that multiphoton microscopy can be used to monitor monocyte subpopulation trafficking in atherosclerotic plaques in living animals. ...
Analyzed the data: ASH HF. Contributed reagents/materials/analysis tools: ASH SP GJR FRM. Wrote the paper: ASH. ...
doi:10.1371/journal.pone.0044823
pmid:23024767
pmcid:PMC3443108
fatcat:brscnqjzmjaajoo5c7dz64emmi
A multiphoton microscope platform for imaging the mouse eye
2012
Molecular Vision
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To demonstrate the ability of multiphoton microscopy to obtain full three-dimensional high-resolution images of the intact mouse eye anterior chamber without need for enucleation. ...
Full three-dimensional image reconstructions of the entire anterior chamber were performed and analyzed using custom software. Multiphoton imaging is a highly promising tool for ophthalmic research. ...
Supported in part by a Bioscience Discovery Evaluation Grant from the State of Colorado and the University of Colorado Technology Transfer Office (Grant No. 11BGF-30). ...
pmid:22815637
pmcid:PMC3398498
fatcat:gxu4jdofmbhjxgdurjgtwopxvu
Multiphoton Microscopy for 3-Dimensional Imaging of Lymphocyte Recruitment Into Apolipoprotein-E-Deficient Mouse Carotid Artery
2007
Circulation
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Images in cardiovascular medicine : multiphoton microscopy for threedimensional imaging of lymphocyte recruitment into apolipoprotein-Edeficient mouse carotid artery. Circulation, 115. E326-E328. ...
In our laboratory, we have established multiphoton scanning microscopy to allow imaging of lymphocytes in real time, in situ, in vivo. 3 Here, we report the 3-dimensional imaging of the entire structure ...
Multiphoton Microscopy for 3-Dimensional Imaging of Lymphocyte Recruitment Into Apolipoprotein-E-Deficient Mouse Carotid Artery T wo recent elegant studies have shown that in apolipoprotein-E-deficient ...
doi:10.1161/circulationaha.106.658492
pmid:17372180
fatcat:iqnqf5nqzrd63oov55srnyzmym
Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex
2015
Microscopy and Microanalysis
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microscopy (ssTEM) reconstruction of 1 mm 3 from the same imaged area. ...
Our long-term goal is to prepare EM samples for a large-scale, dense reconstruction of a block of the mouse visual cortex, where activity was previously recorded by multiphoton Ca 2+ imaging in an awake ...
microscopy (ssTEM) reconstruction of 1 mm 3 from the same imaged area. ...
doi:10.1017/s1431927615007163
fatcat:g6w4unlme5e7na2j2rl7ftoqra
Wide-field multiphoton imaging with TRAFIX
2019
Multiphoton Microscopy in the Biomedical Sciences XIX
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be achieved with 15% of the recorded data, and the position of the beads, with some noise, can be recovered from
5% of the data. ...
Preliminary data for three-photon excitation TRAFIX. Images of 2.1 µm blue fluorescent microspheres imaged
at 1000 nm wavelength. ...
doi:10.1117/12.2508373
fatcat:v35zgt45trf5ba4fsnniyp2d4u
Multifocal multiphoton microscopy: A fast and efficient tool for 3‐D fluorescence imaging
1998
Bioimaging (Bristol. Print)
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Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images. ...
In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cells. ...
Acknowledgments We would like to thank P Lodemann, P Holroyd and Dr W Zuschratter for the preparation of samples and for useful discussions. ...
doi:10.1002/1361-6374(199812)6:4<177::aid-bio3>3.3.co;2-i
fatcat:rcalqxgx7jatjo6syq2pabgf4a
Visual Reality: Using Computer Reconstruction and Animation to Magnify the Microscopist's Perception
1999
Molecular Biology of the Cell
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Gardner, and Jean-Yves Sgro for computing guidance; Melanie Dunn and Geraldine Seydoux for histone-H1::GFP transgenic worms; and James Waddle for discussion of unpublished data. ...
ACKNOWLEDGMENTS I thank John White for advice and for use of microscopes at the Integrated Microscopy Resource; David Wokosin and Victoria Centonze Frohlich for instrument support; Bill Hibbard for discussions ...
Dynamics of cell membrane fusion seen in stereo-4D reconstructions of C. elegans embryos labeled with FM 4 -64 and imaged by multiphoton microscopy (Mohler et al., 1998) . ...
doi:10.1091/mbc.10.10.3061
pmid:10512850
pmcid:PMC25558
fatcat:hdg4web43nc7hhdqk5isykzgdi
Multifocal multiphoton microscopy: A fast and efficient tool for 3-D fluorescence imaging
1998
Bioimaging (Bristol. Print)
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Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images. ...
In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cells. ...
Acknowledgments We would like to thank P Lodemann, P Holroyd and Dr W Zuschratter for the preparation of samples and for useful discussions. ...
doi:10.1002/1361-6374(199812)6:4<177::aid-bio3>3.0.co;2-r
fatcat:syyhcdjn3nfc5novfxvceth72y
Supplementary document for Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency - 4848713.pdf
2020
figshare.com
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Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency: supplemental document Supplementary figures Fig. S1. ...
Black line shows the result of the scaling law fitted on logarithmic scaled data. ( ) follows a scaling law of order n~4.9. ...
Visualization 3. 4D reconstruction of the zebrafish beating heart imaged with 2P-SPIM at 168 frames per second with optimized laser parameters. ...
doi:10.6084/m9.figshare.13003595.v1
fatcat:5ul5hzth5nchzatew5k5zcjnlq
Supplementary document for Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency - 4848713.pdf
2020
figshare.com
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Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency: supplemental document Supplementary figures Fig. S1. ...
Black line shows the result of the scaling law fitted on logarithmic scaled data. ( ) follows a scaling law of order n~4.9. ...
Visualization 3. 4D reconstruction of the zebrafish beating heart imaged with 2P-SPIM at 168 frames per second with optimized laser parameters. ...
doi:10.6084/m9.figshare.13003595.v2
fatcat:6kv347q5fffbzanqvhd336vlye
Image heterogeneity correction in large-area, three-dimensional multiphoton microscopy
2008
Optics Express
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Large-area multiphoton laser scanning microscopy (LMLSM) can be applied in biology and medicine for high sensitivity and resolution tissue imaging. ...
The proposed methodology is demonstrated in correcting multiphoton images of objects imbedded in uniform fluorescent background, lung tissue, and Drosophila larva. ...
Acknowledgment This work is supported by the National Research Program for Genomic Medicine (NRPGM) of the National Science Council in Taiwan and was completed in the Optical Molecular Imaging Microscopy ...
doi:10.1364/oe.16.005107
pmid:18542610
fatcat:kyonlw54ajebvdu5po4izozquu
Hyperglycemia-Induced Abnormalities in Rat and Human Corneas: The Potential of Second Harmonic Generation Microscopy
2012
PLoS ONE
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It provided background-free images of the three-dimensional interwoven distribution of the collagen deposits, with improved contrast compared to confocal microscopy. ...
SHG imaging was compared to confocal microscopy, to histology characterization using conventional staining and transmitted light microscopy and to transmission electron microscopy. ...
Supatto for critical reading of the manuscript and A. Lestini for his precious help for the drawings of the cornea and the orientations of the different sections.
Author Contributions ...
doi:10.1371/journal.pone.0048388
pmid:23139780
pmcid:PMC3489670
fatcat:tzt2kivbz5chhfkil3dusomfzu
Live cell imaging by multifocal multiphoton microscopy
2000
European Journal of Cell Biology
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Multifocal multiphoton microscopy (MMM) permits parallel multiphoton excitation by scanning an array of high numerical aperture foci across a plane in the sample. ...
MMM is particularly suitable for live cell investigations since it combines advantages of standard multiphoton microscopy such as optical sectioning and suppression of out-of-focus phototoxicity with high ...
Jovin for making available to us the technical drawings of the superfusion chamber and T. Lang for useful discussions and critical reading of the manuscript. ...
doi:10.1078/0171-9335-00105
pmid:11089921
fatcat:fuz43o3wijcqzmrdkbwdy3vvz4
Multiphoton fluorescence, second harmonic generation, and fluorescence lifetime imaging of whole cleared mouse organs
2011
Journal of Biomedical Optics
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Multiphoton microscopy of cleared tissue has previously been demonstrated to generate large threedimensional (3D) volumetric image data on entire intact mouse organs using intrinsic tissue fluorescence ...
In addition, we demonstrate the power of multimodal imaging, combining multiphoton fluorescence, second harmonic generation, and lifetime imaging to reveal exceptional morphological detail in an optically ...
A multitude of approaches for three-dimensional reconstruction of tissue sections have been employed including optical coherence tomography, 1, 2 confocal microscopy, 3 multiphoton microscopy (MPM), ...
doi:10.1117/1.3641992
pmid:22029356
fatcat:auwxluaujfaixjytjd52lsbtdu
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