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Image reconstruction of multiphoton microscopy data

Jared M. Doot, Kevin W. Eliceiri, Robert D. Nowak, Rebecca Willett
2009 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro  
The techniques introduced in this paper allow for accurate multi-scale image reconstruction of multi-photon microscopy data.  ...  Multiphoton microscopy (MPM) is a non-invasive imaging modality that can provide high resolution images of fluorescence with low toxicity, high signal to noise ratio and optical sectioning deeper than  ...  We have also started to apply these methods on biological multiphoton data. Our study examined data gathered from a histology slide at various collection times.  ... 
doi:10.1109/isbi.2009.5193171 pmid:22158826 pmcid:PMC3235336 dblp:conf/isbi/DootENW09 fatcat:aangx4ldfjfkxfxd3thiz2rxay

Quantitative Analysis of Monocyte Subpopulations in Murine Atherosclerotic Plaques by Multiphoton Microscopy

Abigail S. Haka, Stephane Potteaux, Haley Fraser, Gwendalyn J. Randolph, Frederick R. Maxfield, Irene Georgakoudi
2012 PLoS ONE  
Citation: Haka AS, Potteaux S, Fraser H, Randolph GJ, Maxfield FR (2012) Quantitative Analysis of Monocyte Subpopulations in Murine Atherosclerotic Plaques by Multiphoton Microscopy.  ...  Next, we demonstrate that multiphoton microscopy can be used to monitor monocyte subpopulation trafficking in atherosclerotic plaques in living animals.  ...  Analyzed the data: ASH HF. Contributed reagents/materials/analysis tools: ASH SP GJR FRM. Wrote the paper: ASH.  ... 
doi:10.1371/journal.pone.0044823 pmid:23024767 pmcid:PMC3443108 fatcat:brscnqjzmjaajoo5c7dz64emmi

A multiphoton microscope platform for imaging the mouse eye

Omid Masihzadeh, Tim C Lei, David A Ammar, Malik Y Kahook, Emily A Gibson
2012 Molecular Vision  
To demonstrate the ability of multiphoton microscopy to obtain full three-dimensional high-resolution images of the intact mouse eye anterior chamber without need for enucleation.  ...  Full three-dimensional image reconstructions of the entire anterior chamber were performed and analyzed using custom software. Multiphoton imaging is a highly promising tool for ophthalmic research.  ...  Supported in part by a Bioscience Discovery Evaluation Grant from the State of Colorado and the University of Colorado Technology Transfer Office (Grant No. 11BGF-30).  ... 
pmid:22815637 pmcid:PMC3398498 fatcat:gxu4jdofmbhjxgdurjgtwopxvu

Multiphoton Microscopy for 3-Dimensional Imaging of Lymphocyte Recruitment Into Apolipoprotein-E-Deficient Mouse Carotid Artery

P. Maffia, B. H. Zinselmeyer, A. Ialenti, S. Kennedy, A. H. Baker, I. B. McInnes, J. M. Brewer, P. Garside
2007 Circulation  
Images in cardiovascular medicine : multiphoton microscopy for threedimensional imaging of lymphocyte recruitment into apolipoprotein-Edeficient mouse carotid artery. Circulation, 115. E326-E328.  ...  In our laboratory, we have established multiphoton scanning microscopy to allow imaging of lymphocytes in real time, in situ, in vivo. 3 Here, we report the 3-dimensional imaging of the entire structure  ...  Multiphoton Microscopy for 3-Dimensional Imaging of Lymphocyte Recruitment Into Apolipoprotein-E-Deficient Mouse Carotid Artery T wo recent elegant studies have shown that in apolipoprotein-E-deficient  ... 
doi:10.1161/circulationaha.106.658492 pmid:17372180 fatcat:iqnqf5nqzrd63oov55srnyzmym

Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex

JoAnn Buchanan, Marc Takeno, Tanya Daigle, Adam Bleckert, Daniel Bumbarger, Agnes Bodor, Derrick Brittain, Ali Cetin, Nuno Macarico da Costa
2015 Microscopy and Microanalysis  
microscopy (ssTEM) reconstruction of 1 mm 3 from the same imaged area.  ...  Our long-term goal is to prepare EM samples for a large-scale, dense reconstruction of a block of the mouse visual cortex, where activity was previously recorded by multiphoton Ca 2+ imaging in an awake  ...  microscopy (ssTEM) reconstruction of 1 mm 3 from the same imaged area.  ... 
doi:10.1017/s1431927615007163 fatcat:g6w4unlme5e7na2j2rl7ftoqra

Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán, Kishan Dholakia, Mingzhou Chen, Philip Wijesinghe, Ammasi Periasamy, Peter T. So, Karsten König
2019 Multiphoton Microscopy in the Biomedical Sciences XIX  
be achieved with 15% of the recorded data, and the position of the beads, with some noise, can be recovered from 5% of the data.  ...  Preliminary data for three-photon excitation TRAFIX. Images of 2.1 µm blue fluorescent microspheres imaged at 1000 nm wavelength.  ... 
doi:10.1117/12.2508373 fatcat:v35zgt45trf5ba4fsnniyp2d4u

Multifocal multiphoton microscopy: A fast and efficient tool for 3‐D fluorescence imaging

Martin Straub, Stefan W Hell
1998 Bioimaging (Bristol. Print)  
Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images.  ...  In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cells.  ...  Acknowledgments We would like to thank P Lodemann, P Holroyd and Dr W Zuschratter for the preparation of samples and for useful discussions.  ... 
doi:10.1002/1361-6374(199812)6:4<177::aid-bio3>3.3.co;2-i fatcat:rcalqxgx7jatjo6syq2pabgf4a

Visual Reality: Using Computer Reconstruction and Animation to Magnify the Microscopist's Perception

William A. Mohler, Jennifer Lippincott-Schwartz
1999 Molecular Biology of the Cell  
Gardner, and Jean-Yves Sgro for computing guidance; Melanie Dunn and Geraldine Seydoux for histone-H1::GFP transgenic worms; and James Waddle for discussion of unpublished data.  ...  ACKNOWLEDGMENTS I thank John White for advice and for use of microscopes at the Integrated Microscopy Resource; David Wokosin and Victoria Centonze Frohlich for instrument support; Bill Hibbard for discussions  ...  Dynamics of cell membrane fusion seen in stereo-4D reconstructions of C. elegans embryos labeled with FM 4 -64 and imaged by multiphoton microscopy (Mohler et al., 1998) .  ... 
doi:10.1091/mbc.10.10.3061 pmid:10512850 pmcid:PMC25558 fatcat:hdg4web43nc7hhdqk5isykzgdi

Multifocal multiphoton microscopy: A fast and efficient tool for 3-D fluorescence imaging

Martin Straub, Stefan W Hell
1998 Bioimaging (Bristol. Print)  
Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images.  ...  In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cells.  ...  Acknowledgments We would like to thank P Lodemann, P Holroyd and Dr W Zuschratter for the preparation of samples and for useful discussions.  ... 
doi:10.1002/1361-6374(199812)6:4<177::aid-bio3>3.0.co;2-r fatcat:syyhcdjn3nfc5novfxvceth72y

Supplementary document for Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency - 4848713.pdf

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia FERRER ORTAS, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto
2020 figshare.com  
Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency: supplemental document Supplementary figures Fig. S1.  ...  Black line shows the result of the scaling law fitted on logarithmic scaled data. ( ) follows a scaling law of order n~4.9.  ...  Visualization 3. 4D reconstruction of the zebrafish beating heart imaged with 2P-SPIM at 168 frames per second with optimized laser parameters.  ... 
doi:10.6084/m9.figshare.13003595.v1 fatcat:5ul5hzth5nchzatew5k5zcjnlq

Supplementary document for Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency - 4848713.pdf

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia FERRER ORTAS, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto
2020 figshare.com  
Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency: supplemental document Supplementary figures Fig. S1.  ...  Black line shows the result of the scaling law fitted on logarithmic scaled data. ( ) follows a scaling law of order n~4.9.  ...  Visualization 3. 4D reconstruction of the zebrafish beating heart imaged with 2P-SPIM at 168 frames per second with optimized laser parameters.  ... 
doi:10.6084/m9.figshare.13003595.v2 fatcat:6kv347q5fffbzanqvhd336vlye

Image heterogeneity correction in large-area, three-dimensional multiphoton microscopy

Vladimir A. Hovhannisyan, Ping-Jung Su, Yang-Fang Chen, Chen Y. Dong
2008 Optics Express  
Large-area multiphoton laser scanning microscopy (LMLSM) can be applied in biology and medicine for high sensitivity and resolution tissue imaging.  ...  The proposed methodology is demonstrated in correcting multiphoton images of objects imbedded in uniform fluorescent background, lung tissue, and Drosophila larva.  ...  Acknowledgment This work is supported by the National Research Program for Genomic Medicine (NRPGM) of the National Science Council in Taiwan and was completed in the Optical Molecular Imaging Microscopy  ... 
doi:10.1364/oe.16.005107 pmid:18542610 fatcat:kyonlw54ajebvdu5po4izozquu

Hyperglycemia-Induced Abnormalities in Rat and Human Corneas: The Potential of Second Harmonic Generation Microscopy

Gaël Latour, Laura Kowalczuk, Michèle Savoldelli, Jean-Louis Bourges, Karsten Plamann, Francine Behar-Cohen, Marie-Claire Schanne-Klein, Irene Georgakoudi
2012 PLoS ONE  
It provided background-free images of the three-dimensional interwoven distribution of the collagen deposits, with improved contrast compared to confocal microscopy.  ...  SHG imaging was compared to confocal microscopy, to histology characterization using conventional staining and transmitted light microscopy and to transmission electron microscopy.  ...  Supatto for critical reading of the manuscript and A. Lestini for his precious help for the drawings of the cornea and the orientations of the different sections. Author Contributions  ... 
doi:10.1371/journal.pone.0048388 pmid:23139780 pmcid:PMC3489670 fatcat:tzt2kivbz5chhfkil3dusomfzu

Live cell imaging by multifocal multiphoton microscopy

M STRAUB
2000 European Journal of Cell Biology  
Multifocal multiphoton microscopy (MMM) permits parallel multiphoton excitation by scanning an array of high numerical aperture foci across a plane in the sample.  ...  MMM is particularly suitable for live cell investigations since it combines advantages of standard multiphoton microscopy such as optical sectioning and suppression of out-of-focus phototoxicity with high  ...  Jovin for making available to us the technical drawings of the superfusion chamber and T. Lang for useful discussions and critical reading of the manuscript.  ... 
doi:10.1078/0171-9335-00105 pmid:11089921 fatcat:fuz43o3wijcqzmrdkbwdy3vvz4

Multiphoton fluorescence, second harmonic generation, and fluorescence lifetime imaging of whole cleared mouse organs

Sam Vesuna, Richard Torres, Michael J. Levene
2011 Journal of Biomedical Optics  
Multiphoton microscopy of cleared tissue has previously been demonstrated to generate large threedimensional (3D) volumetric image data on entire intact mouse organs using intrinsic tissue fluorescence  ...  In addition, we demonstrate the power of multimodal imaging, combining multiphoton fluorescence, second harmonic generation, and lifetime imaging to reveal exceptional morphological detail in an optically  ...  A multitude of approaches for three-dimensional reconstruction of tissue sections have been employed including optical coherence tomography, 1, 2 confocal microscopy, 3 multiphoton microscopy (MPM),  ... 
doi:10.1117/1.3641992 pmid:22029356 fatcat:auwxluaujfaixjytjd52lsbtdu
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