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Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty

Renata Alleva, Andrea Tognù, Marco Tomasetti, Maria Serena Benassi, Laura Pazzaglia, Hanna van Oven, Ettore Viganò, Nicola De Simone, Ilaria Pacini, Sandra Giannone, Sanjin Gagic, Raffaele Borghi (+3 others)
2019 PLoS ONE  
Methodology: Andrea Tognù, Marco Tomasetti, Laura Pazzaglia, Ilaria Pacini, Raffaele Borghi. administration: Battista Borghi. Resources: Battista Borghi.  ...  Writing -review & editing: Battista Borghi.  ... 
doi:10.1371/journal.pone.0219113 pmid:31344051 pmcid:PMC6657832 fatcat:4yiyr4tburcu7mhas5nnywrbvi

Fad diets and their effect on urinary stone formation

Antonio Nouvenne, Andrea Ticinesi, Ilaria Morelli, Loredana Guida, Loris Borghi, Tiziana Meschi
2014 Translational Andrology and Urology  
The influence of unhealthy dietary habits on urinary stone formation has been widely recognized in literature. Dietary advice is indeed the cornerstone prescription for prevention of nephrolithiasis as well. However, only a small amount of medical literature has addressed the influence of popular or fad diets, often self-prescribed for the management of obesity and overweight or for cultural beliefs, on the risk of kidney stones. Thereby in this paper we analyze the current knowledge on the
more » ... cts of some popular diets on overall lithogenic risk. High-protein diets, like Dukan diet, raise some concerns, since animal proteins are able to increase urinary calcium and to decrease urinary citrate excretion, thus leading to a high overall lithogenic risk. Low-carbohydrate diets, like Atkins diet or zone diet, may have a protective role against kidney stone formation, but there are also evidences stating that this dietary approach may rise calciuria and decrease citraturia, since it is generally associated to a relatively high intake of animal proteins. Vegan diet can be harmful for urinary stone disease, especially for the risk of hyperuricemia and micronutrient deficiencies, even if only few studies have addressed this specific matter. On the other side, the benefits of a lacto-ovo-vegetarian diet on kidney stone prevention have been largely emphasized, provided that the intake of calcium and oxalate is balanced. Traditional Mediterranean diet should exert a protective effect on nephrolithiasis as well, even if specific studies have not been carried out yet. High phytate and antioxidant content of this diet have however demonstrated to be beneficial in preventing the formation of new or recurrent calculi. Anyway, at the current state of knowledge, the most effective dietary approach to prevent kidney stone disease is a mild animal protein restriction, a balanced intake of carbohydrates and fats and a high intake of fruit and vegetables. Other fundamental aspects, which are often neglected in fad diets, are a normal intake of milk and dairy products and salt restriction. All these nutritional aspects should be greatly taken into account when patients who are willing to undergo fad or commercial diets ask for dietary advice.
doi:10.3978/j.issn.2223-4683.2014.06.01 pmid:26816783 pmcid:PMC4708571 fatcat:kwksxhytz5egldxklh5r3wujli

Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations

Pier Luigi Meroni, Nicola Bizzaro, Ilaria Cavazzana, Maria Orietta Borghi, Angela Tincani
2014 BMC Medicine  
Anti-nuclear antibody (ANA) assay is a screening test used for almost all autoimmune rheumatic diseases, and in a number of these cases, it is a diagnostic/classification parameter. In addition, ANA is also a useful test for additional autoimmune disorders. The indirect immunofluorescence technique on monolayers of cultured epithelial cells is the current recommended method because it has higher sensitivity than solid phase assays. However, the technique is time-consuming and requires skilled
more » ... erators. Automated ANA reading systems have recently been developed, which offer the advantage of faster and much easier performance as well as better harmonization in the interpretation of the results. Preliminary validation studies of these systems have given promising results in terms of analytical specificity and reproducibility. However, these techniques require further validation in clinical studies and need improvement in their recognition of mixed or less common staining patterns.
doi:10.1186/1741-7015-12-38 pmid:24589329 pmcid:PMC3939809 fatcat:efdlchxkgzbhvieiywtqshqcna

Characterizing TMS-EEG perturbation indexes using signal energy: initial study on Alzheimer's Disease classification [article]

Alexandra-Maria Tautan, Elias Casula, Ilaria Borghi, Michele Maiella, Sonia Bonni, Marilena Minei, Martina Assogna, Bogdan Ionescu, Giacomo Koch, Emiliano Santarnecchi
2022 arXiv   pre-print
, Gordon Center for Medical imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA (, 3 Ilaria  ...  Borghi, Michele Maiella, Sonia Bonni, Marilena Minei, Martina Assogna, Elias Casula and Giacomo Koch are with the Santa Lucia Foundation, Via Ardeatina 306354, 00179, Rome, Italy (e-mail:  ... 
arXiv:2205.03241v1 fatcat:uyygcdksnnc53jg7yyntznmlti

Antimicrobial Efficacy of Five Probiotic Strains Against Helicobacter pylori

Ilaria Maria Saracino, Matteo Pavoni, Laura Saccomanno, Giulia Fiorini, Valeria Pesci, Claudio Foschi, Giulia Piccirilli, Giulia Bernardini, John Holton, Natale Figura, Tiziana Lazzarotto, Claudio Borghi (+1 others)
2020 Antibiotics  
Treatment of Helicobacter pylori (H. pylori) infection is a challenge for clinicians. The large increase in drug-resistant strains makes the formulation of new therapeutic strategies fundamental. The frequent onset of side effects during antibiotic treatment (mainly due to intestinal dysbiosis) should not be underestimated as it may cause the interruption of treatment, failure of H. pylori eradication and clonal selection of resistant bacteria. Probiotic integration during antibiotic treatment
more » ... an exert a dual function: a direct antagonistic effect on H. pylori and a balancing effect on dysbiosis. Therefore, it fulfills the definition of a new therapeutic strategy to successfully treat H. pylori infection. Data reported in literature give promising but discrepant results. Aim: To assess in vitro bacteriostatic and bactericidal activity of probiotic strains against H. pylori. Materials and methods: L. casei, L. paracasei, L. acidophilus, B. lactis and S. thermophilus strains were used. Agar well diffusion and time-kill curves were carried out to detect bacteriostatic and bactericidal activity, respectively. Results: All probiotic strains showed both bacteriostatic and bactericidal activity vs. H. pylori. Conclusions: Such findings prompted us to plan a protocol of treatment in which probiotics are given to infected patients in association with antibiotic therapy.
doi:10.3390/antibiotics9050244 pmid:32403331 pmcid:PMC7277513 fatcat:fuidbklnjnhq3jgk2lwoz3b574

Next Generation Sequencing for the Prediction of the Antibiotic Resistance in Helicobacter pylori: A Literature Review

Ilaria Maria Saracino, Matteo Pavoni, Angelo Zullo, Giulia Fiorini, Tiziana Lazzarotto, Claudio Borghi, Dino Vaira
2021 Antibiotics  
and aims: Only a few antimicrobials are effective against H. pylori, and antibiotic resistance is an increasing problem for eradication therapies. In 2017, the World Health Organization categorized clarithromycin resistant H. pylori as a "high-priority" bacterium. Standard antimicrobial susceptibility testing can be used to prescribe appropriate therapies but is currently recommended only after the second therapeutic failure. H. pylori is, in fact, a "fastidious" microorganism; culture methods
more » ... re time-consuming and technically challenging. The advent of molecular biology techniques has enabled the identification of molecular mechanisms underlying the observed phenotypic resistance to antibiotics in H. pylori. The aim of this literature review is to summarize the results of original articles published in the last ten years, regarding the use of Next Generation Sequencing, in particular of the whole genome, to predict the antibiotic resistance in H. pylori.Methods: a literature research was made on PubMed. The research was focused on II and III generation sequencing of the whole H. pylori genome. Results: Next Generation Sequencing enabled the detection of novel, rare and complex resistance mechanisms. The prediction of resistance to clarithromycin, levofloxacin and amoxicillin is accurate; for other antimicrobials, such as metronidazole, rifabutin and tetracycline, potential genetic determinants of the resistant status need further investigation.
doi:10.3390/antibiotics10040437 pmid:33919811 pmcid:PMC8070836 fatcat:swbbfusfjbhcxbkkfhyqxwmuge

Anti-Phospholipid Antibodies and Coronavirus Disease 2019: Vaccination Does Not Trigger Early Autoantibody Production in Healthcare Workers

Maria Orietta Borghi, Maria Orietta Borghi, Mauro Bombaci, Caterina Bodio, Paola Adele Lonati, Andrea Gobbini, Mariangela Lorenzo, Erminio Torresani, Antonella Dubini, Ilaria Bulgarelli, Francesca Solari, Francesca Pregnolato (+12 others)
2022 Frontiers in Immunology  
A molecular mimicry between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human proteins supports the possibility that autoimmunity takes place during coronavirus disease 2019 (COVID-19) contributing to tissue damage. For example, anti-phospholipid antibodies (aPL) have been reported in COVID-19 as a result of such mimicry and thought to contribute to the immunothrombosis characteristic of the disease. Consistently, active immunization with the virus spike protein may elicit
more » ... he production of cross-reactive autoantibodies, including aPL. We prospectively looked at the aPL production in healthcare workers vaccinated with RNA- (BNT162b2, n. 100) or adenovirus-based vaccines (ChAdOx1, n. 50). Anti-cardiolipin, anti-beta2 glycoprotein I, anti-phosphatidylserine/prothrombin immunoglobulin G (IgG), IgA, and IgM before and after vaccination were investigated. Anti-platelet factor 4 immunoglobulins were also investigated as autoantibodies associated with COVID-19 vaccination. Additional organ (anti-thyroid) and non-organ (anti-nuclear) autoantibodies and IgG against human proteome were tested as further post-vaccination autoimmunity markers. The antibodies were tested one or three months after the first injection of ChAdOx1 and BNT162b2, respectively; a 12-month clinical follow-up was also performed. Vaccination occasionally induced low titers of aPL and other autoantibodies but did not affect the titer of pre-existing autoantibodies. No significant reactivities against a microarray of approximately 20,000 human proteins were found in a subgroup of ChAdOx1-vaccinees. Consistently, we did not record any clinical manifestation theoretically associated with an underlying autoimmune disorder. The data obtained after the vaccination (two doses for the RNA-based and one dose for the adenovirus-based vaccines), and the clinical follow-up are not supporting the occurrence of an early autoimmune response in this cohort of healthcare workers.
doi:10.3389/fimmu.2022.930074 pmid:35911726 pmcid:PMC9334668 doaj:5fb6aabe8703441cb969853cdbfd7ca0 fatcat:m6sa3cbt3rdvddbja5rc2ir6ki

Effects of Treatment with Zofenopril in Men and Women with Acute Myocardial Infarction: Gender Analysis of the SMILE Program

Flavia Franconi, Stefano Omboni, Ettore Ambrosioni, Giorgio Reggiardo, Ilaria Campesi, Claudio Borghi, Gianpaolo Reboldi
2014 PLoS ONE  
the SMILE studies proved the prognostic benefit of zofenopril vs. placebo or other ACE-inhibitors (ACEIs) in post-acute myocardial infarction (AMI). In this retrospective pooled analysis of these studies we assessed whether the zofenopril effect is influenced by gender. Methods: the four double-blind, randomized, parallel-group SMILE studies, compared the efficacy and safety of 6-48 week treatment with zofenopril 60 mg/day with that of placebo, lisinopril 10 mg/day or ramipril 10 mg/day in 3630
more » ... AMI patients. This pooled analysis compared treatment efficacy (1-year combined occurrence of death or hospitalization for CV causes) in 2733 men and 897 women. Results: women were older than men, had a higher prevalence of diabetes and of other major CV risk factors. The risk of a major CV event was significantly larger for women (23% vs. 17% men, p,0.001). Between-gender risk difference was more marked for people living in Southern (+54%) than in Northern Europe (+12%). In both genders zofenopril similarly reduced the 1-year risk of CV morbidity and mortality vs. placebo (239% men, p = 0.0001; 240% women, p = 0.005). The risk reduction was more marked with zofenopril than with the other ACEIs, particularly in men (227%, p = 0.012; women: 214%, p = 0.479). The drug safety profile was similar between genders in zofenopril-treated patients, while it was worse in women treated with other ACEIs. Conclusions: post-AMI women are at higher risk of CV complications than men, particularly when living in Mediterranean countries. Their response to ACE-inhibition varies according to the type of drug and is usually better in men.
doi:10.1371/journal.pone.0111558 pmid:25364906 pmcid:PMC4218766 fatcat:bhwcawdeyfa2pmhupqi3kqaqle

Cardiomyopathy in a male patient with neutropenia and growth delay

Veronica Folsi, Nunzia Miglietti, Annamaria Lombardi, Sara Boccacci, Tatiana Utyatnikova, Chiara Donati, Livia Squassabia, Laura Gazzola, Ilaria Bosio, Adele Borghi, Veronica Grassi, Lucia D Notarangelo (+1 others)
2014 Italian Journal of Pediatrics  
Neutropenia encompasses a family of neutropenic disorders, both permanent and intermittent, ranging from severe (<500 neutrophils/mm 3 ) to mild (500-1500 neutrophils/mm 3 ), which may also affect other organ systems such as the pancreas, central nervous system, heart, muscle and skin. Neutropenia can lead to life-threatening pyogenic infections whose severity is roughly inversely proportional to the circulating neutrophil counts. When neutropenia is detected, an attempt should be made to
more » ... ish the etiology, and to distinguish acquired forms (the most frequent, including post viral neutropenia and autoimmune neutropenia) and congenital forms (rare disorders) that may be either isolated or part of a complex rare genetic disease. We report on a male patient initially diagnosed with isolated neutropenia who later turned out to be affected with Barth syndrome, a rare complex inherited disorder.
doi:10.1186/1824-7288-40-45 pmid:24887148 pmcid:PMC4046054 fatcat:66fius4dqzcobc3cb2m6gvc4hm

Coexisting Heart Failure and Chronic Obstructive Pulmonary Disease: Report of Two Cases Treated with Indacaterol/Glycopyrronium

Eugenio Roberto Cosentino, Daniela Degli Esposti, Rinaldo Miceli, Crescenzio Bentivenga, Matteo Landolfo, Ilaria Ricci Iamino, Luca Spinardi, Gianluigi Magri, Claudio Borghi
2018 Respiration  
Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity and mortality worldwide, and it is associated with a high economic burden. Heart failure shares some general symptoms with COPD; thus, diagnosing COPD is difficult in subjects with a history of heart failure, and spirometry is mandatory for confirmation. Moreover, COPD is a highly prevalent comorbidity negatively impacting the outcome of heart failure patients. We document here the treatment with
more » ... m in 2 patients with concomitant COPD and heart failure. Overall, the combination of indacaterol and glycopyrronium resulted in a reciprocal potentiation with a maximal bronchodilatory effect.
doi:10.1159/000487169 pmid:29705784 fatcat:rothdii3tfhdljfk5ewe4zh2gm

Is Gut Microbiota a Key Player in Epilepsy Onset? A Longitudinal Study in Drug-Naive Children

Camilla Ceccarani, Ilaria Viganò, Emerenziana Ottaviano, Maria Gaia Redaelli, Marco Severgnini, Aglaia Vignoli, Elisa Borghi
2021 Frontiers in Cellular and Infection Microbiology  
INTRODUCTION The microbiota-gut-brain axis has recently gained growing interest as a new frontier for explaining the complex features of different neurological diseases (Borghi and Vignoli, 2019; Cryan  ...  biodiversity has been reported as one of the first signs of gut microbiota alterations (Mosca et al., 2016) , and has been observed in neurodevelopmental diseases characterized by seizures as a comorbidity (Borghi  ... 
doi:10.3389/fcimb.2021.749509 pmid:34926315 pmcid:PMC8677705 fatcat:pj6xektz2ffwtndrxvosn2qy6y

Congenital idiopathic talipes equinovarus before and after walking age: observations and strategy of treatment from a series of 88 cases

Cesare Faldini, Francesco Traina, Matteo Nanni, Ilaria Sanzarello, Raffaele Borghi, Fabrizio Perna
2015 Journal of Orthopaedics and Traumatology  
We reviewed a series of newborns, toddlers and ambulating children affected by idiopathic congenital talipes equinovarus (clubfoot). Taking into account the time of diagnosis, stiffness of the deformity and walking age, nonsurgical or surgical treatment was considered. This study reports clinical outcomes, early complications and relapse at mid-term follow-up. Materials and methods Fifty-two clubfeet were diagnosed at birth, 12 in non-ambulating children aged between 4 and 12 months and 24 in
more » ... bulating children. Feet were classified using the Pirani score. Newborns and toddlers were treated with serial casting (Ponseti); however, toddlers also underwent open Achilles tendon lengthening (2 feet) and posteromedial release (3 feet). In all ambulating children, surgical treatment was always performed: selective medial release combined with cuboid subtraction osteotomy (1 foot), posteromedial release (6 feet), and posteromedial release combined with cuboid subtraction osteotomy (17 feet). Results The average follow-up was 5 years (1-6 years). In newborns treated with Ponseti, the results were excellent in 42 feet, good in 6, and poor in 4. In non-ambulating children, the results were excellent in 9 feet, and good in 3. In ambulating children, the results were excellent in 5 feet, good in 16, and poor in 3. No major complications were reported. No overcorrections were observed. The need for open surgery was higher in cases of delayed treatment. In cases of relapse, re-casting and/or more extensive surgery was considered. Conclusions Early treatment enables a high rate of good correction to be obtained with serial casting and limited surgery. Conversely, if the deformity is observed after walking age surgery should be considered. Serial casting in cases of late observation and relapse have demonstrated encouraging results. Level of evidence IV.
doi:10.1007/s10195-015-0377-4 pmid:26409466 pmcid:PMC4805627 fatcat:imzfxitbe5f6hkpajf47ekghzy

Irrelevance of Panton-Valentine leukocidin in hidradenitis suppurativa: results from a pilot, observational study

Monica Corazza, Alessandro Borghi, Vincenzo Bettoli, Roberto Pora, Ilaria Bononi, Elisa Mazzoni, Elisa Mazzola, Silva Saraceni, Martina Maritati, Carlo Contini
2020 European Journal of Clinical Microbiology and Infectious Diseases  
Borghi: design of the study, analysis and interpretation of data, drafting of the manuscript, critical revision of the manuscript. V.  ...  Bacterial colonization of Monica Corazza, Alessandro Borghi, and Vincenzo Bettoli contributed equally to this study and share first authorship.  ... 
doi:10.1007/s10096-020-04002-7 pmid:32767177 fatcat:xdnggivlszaihdpz6gf7tnxwb4

Seasonal betacoronavirus antibodies expansion post BNT161b2 vaccination associates with reduced SARS-CoV-2 VoCs neutralization [article]

Stefania Dispinseri, Ilaria Marzinotto, Cristina Brigatti, Maria Franca Pirillo, Monica Tolazzi, Elena Bazzigaluppi, Andrea Canitano, Martina Borghi, Alessandra Gallinaro, Roberta Caccia, Riccardo Vercesi, Paul F McKay (+7 others)
2021 medRxiv   pre-print
SARS-CoV-2 vaccination is known to induce antibodies that recognize also variants of concerns (VoCs) of the virus. However, epidemiological and laboratory evidences indicate that these antibodies have a reduce neutralization ability against VoCs. We studied binding and neutralizing antibodies against the Spike RBD and S2 domains of the Wuhan-Hu-1 virus and its alpha and beta VoCs and of seasonal betacoronaviruses (HKU1 and OC43) in a cohort of 31 health care workers vaccinated with
more » ... rnaty and prospectively followed post-vaccination. The study of sequential samples collected up to 64 days post-vaccination showed that serological assays measuring IgG against Wuhan-Hu-1 antigens were a poor proxy for VoCs neutralization. In addition, in subjects who had asymptomatic or mild COVID-19 prior to vaccination the loss of nAbs following disease can be rapid and protection from re-infection post-vaccination is often no better than in naïve subjects. Interestingly, in health care workers naïve for SARS-CoV-2 infection, vaccination induced a rapid and transient reactivation of pre-existing seasonal coronaviruses IgG responses that was associated with a subsequent reduced ability to neutralize some VoCs.
doi:10.1101/2021.08.15.21262000 fatcat:bs4c5z2olvgllbh3zotws5sq54

Preliminary study on the impact of EEG density on TMS-EEG classification in Alzheimer's disease [article]

Alexandra-Maria Tautan, Elias Casula, Ilaria Borghi, Michele Maiella, Sonia Bonni, Marilena Minei, Martina Assogna, Bogdan Ionescu, Giacomo Koch, Emiliano Santarnecchi
2022 arXiv   pre-print
No works have been identified that use TMS in combination with recorded EEG response to classify AD patients. 3Ilaria Borghi, Michele Maiella, Sonia Bonni, Marilena Minei, Martina Assogna, Elias Casula  ... 
arXiv:2206.07492v2 fatcat:3w4yu2ivyfdjtile7gsom5lgbu
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