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Identification and characterization of neutrophil extracellular trap shapes in flow cytometry

Brandon Ginley, Tiffany Emmons, Prabhu Sasankan, Constantin Urban, Brahm H. Segal, Pinaki Sarder, Metin N. Gurcan, John E. Tomaszewski
2017 Medical Imaging 2017: Digital Pathology  
We propose an automated pipeline for quantitatively detecting neutrophil and NET shapes captured using a flow cytometry-imaging system.  ...  Neutrophil extracellular trap (NET) formation is an alternate immunologic weapon used mainly by neutrophils.  ...  ACKNOWLEDGMENTS The computational work of the project was supported by the faculty startup funds from the Pathology and Anatomical Sciences Department, Jacobs School of Medicine and Biomedical Sciences  ... 
doi:10.1117/12.2254680 dblp:conf/midp/GinleyESUSS17 fatcat:ibj47uuvkrfpxabqqi4md5l5ra

Rapid Quantification of NETs In Vitro and in Whole Blood Samples by Imaging Flow Cytometry

Patrick M. Lelliott, Masatoshi Momota, Michelle S.J. Lee, Etsushi Kuroda, Norifumi Iijima, Ken J. Ishii, Cevayir Coban
2019 Cytometry Part A  
Neutrophil extracellular trap (NET) formation involves the release of DNA outside the cell to neutralize pathogens.  ...  Techniques such as live microscopy, flow cytometry, and intravital imaging allow the characterization of NETs, but these either cannot be applied in vivo, lack specificity or require invasive procedures  ...  This work was supported by grants from the JSPS Grants-in-Aid for Scientific Research (B grant no. 16H05181 to C.C., JSPS fellows grant JP17F17117 to P.M.L.), Japan Agency for Medical Research and Development  ... 
doi:10.1002/cyto.a.23767 pmid:30985081 fatcat:3c56u7fldfbu7kq4dub77iqslu

The twilight zone: plasticity and mixed ontogeny of neutrophil and eosinophil granulocyte subsets

Sergejs Berdnikovs
2021 Seminars in Immunopathology  
With greater marker coverage by multi-color flow cytometry and single-cell level sequencing of granulocyte populations, novel phenotypes of these cells began to emerge.  ...  This includes reports of neutrophils with features of eosinophils, monocytes and dendritic cells, and eosinophil subsets expressing neutrophil markers.  ...  CD15, CD66b, and CD16 are used in flow cytometry identification of circulating human neutrophils.  ... 
doi:10.1007/s00281-021-00862-z pmid:34009400 fatcat:irr6uviclzgwblonojx2oji4ja

Neutrophil extracellular trap clearance by synovial macrophages in gout

Ji Hye Jeong, Su Jin Choi, Soo Min Ahn, Ji Seon Oh, Yong-Gil Kim, Chang-Keun Lee, Bin Yoo, Seokchan Hong
2021 Arthritis Research & Therapy  
., the central etiological factors in gouty arthritis, induce the formation of neutrophil extracellular traps (NETs).  ...  In addition, the correlations between CD14+ cells, MPO-dsDNA complexes, and expression of pro- and anti-inflammatory cytokines were analyzed in the synovial fluid CD14+ macrophages of patients with gouty  ...  Acknowledgements We thank the confocal microscopy core facility at the Convergence Medicine research Center (CREDIT) of Asan Medical Center for technical assistance with live-cell imaging.  ... 
doi:10.1186/s13075-021-02472-4 pmid:33741037 fatcat:whyrdnnpfbhqpjrqqbhqmgvoyq

Factor H Binds to Extracellular DNA Traps Released from Human Blood Monocytes in Response to Candida albicans

Luke D. Halder, Mahmoud A. Abdelfatah, Emeraldo A. H. Jo, Ilse D. Jacobsen, Martin Westermann, Niklas Beyersdorf, Stefan Lorkowski, Peter F. Zipfel, Christine Skerka
2017 Frontiers in Immunology  
Extracellular traps from both monocytes and neutrophils activate complement and C3b is deposited.  ...  MoET induction differs from extracellular trap formation in macrophages as MoETs are not triggered by simvastatin, an inhibitor of cholesterol synthesis and inducer of extracellular traps in macrophages  ...  Phagocytosis of C. albicans by monocytes was analyzed by flow cytometry.  ... 
doi:10.3389/fimmu.2016.00671 pmid:28133459 pmcid:PMC5233719 fatcat:dwj7q3m5kba6jim5ldfbnzwhxy

Netome: The Molecular Characterization of Neutrophil Extracellular Traps (NETs) [article]

David Scieszka, Yi-Han Lin, Weizhong Li, Saibyasachi Choudhury, Yanbao Yu, Marcelo Freire
2020 bioRxiv   pre-print
Neutrophil Extracellular Traps (NETs) act as antimicrobial agents triggering immune signaling through the release of the nuclear content into the extracellular space.  ...  The release of neutrophil extracellular DNA aims to control invasion by bacteria, viruses, fungi, and tissue damage.  ...  Neutrophil 25 extracellular traps kill bacteria. Science 2004; 303: 1532-1535. 26 2 Zuo Y, Yalavarthi S, Shi H, Gockman K, Zuo M, Madison JA et al. Neutrophil extracellular 27 traps in COVID-19.  ... 
doi:10.1101/2020.05.18.102772 fatcat:brk4as4kgjdcjbp5ongnqsy554

Systemic and mucosal mobilization of granulocyte subsets during lentivirus infection [article]

Rhianna Jones, Cordelia Manickam, Daniel R Ram, Kyle Kroll, Brady Hueber, Griffin Woolley, Spandan V Shah, Scott Smith, Valerie Varner, R. Keith Reeves
2021 bioRxiv   pre-print
Granulocytes mediate broad immunoprotection through phagocytosis, extracellular traps, release of cytotoxic granules, antibody effector functions and recruitment of other immune cells against pathogens  ...  Interestingly, elevated frequencies of circulating neutrophils, colorectal neutrophils, and colorectal eosinophils were all observed in chronic lentivirus disease.  ...  Wira, and Marta Rodriguez-Garcia. 6 " 6 Neutrophil Extracellular Traps Prevent HIV Infection in the Female Genital Tract."  ... 
doi:10.1101/2021.03.19.436235 fatcat:77njzr7pfbg5rgewas45f3ikfi

Identification of a subset of immunosuppressive P2RX1-negative neutrophils in pancreatic cancer liver metastasis

Xu Wang, Li-Peng Hu, Wei-Ting Qin, Qin Yang, De-Yu Chen, Qing Li, Kai-Xia Zhou, Pei-Qi Huang, Chun-Jie Xu, Jun Li, Lin-Li Yao, Ya-Hui Wang (+8 others)
2021 Nature Communications  
Here, we show that a subset of P2RX1-negative neutrophils accumulate in clinical and murine PDAC liver metastases.  ...  However, the specific phenotypes, functions and regulatory mechanisms of neutrophils in PDAC liver metastases remain unknown.  ...  Neutrophil extracellular traps (NETs) in promoting tumor metastasis have attracted much attention recently 22, [32] [33] [34] .  ... 
doi:10.1038/s41467-020-20447-y pmid:33420030 fatcat:cakxii6axzb3jorfi2thouk2hi

A novel image-based quantitative method for the characterization of NETosis

Wenpu Zhao, Darin K. Fogg, Mariana J. Kaplan
2015 JIM - Journal of Immunological Methods  
It is characterized by a stepwise progression of chromatin decondensation, membrane rupture, and release of bactericidal DNA-based structures called neutrophil extracellular traps (NETs).  ...  Use of imaging flow cytometry allows automated, quantitative and rapid analysis of subcellular morphology and texture, and introduces the potential for further investigation using NETosis as a biomarker  ...  Multispectral Imaging Flow Cytometry (MIFC) combines features of fluorescence microscopy and flow cytometry by providing unbiased acquisition and analysis of large number of images from cells in suspension  ... 
doi:10.1016/j.jim.2015.04.027 pmid:26003624 pmcid:PMC4522197 fatcat:idmht6nngnhsrnuoklwosn6vnq

Neutrophil extracellular traps and fibrocytes in ST-segment elevation myocardial infarction

Thomas M. Hofbauer, Andreas Mangold, Thomas Scherz, Veronika Seidl, Adelheid Panzenböck, Anna S. Ondracek, Julian Müller, Matthias Schneider, Thomas Binder, Lena Hell, Irene M. Lang
2019 Basic Research in Cardiology  
Fibrocytes were characterized in whole blood by flow cytometry, and in culprit site thrombi and myocardium by immunofluorescence.  ...  Neutrophil extracellular traps (NETs) have been shown to enhance atherothrombosis and stimulate fibroblast function. We analyzed the effects of NETs on cardiac remodeling after STEMI.  ...  Fibrocyte activation by neutrophil extracellular traps (NETs) in vitro.  ... 
doi:10.1007/s00395-019-0740-3 pmid:31312919 pmcid:PMC6647191 fatcat:23j3zylxmbb35ecdqwjaltshlm

Neutrophil Subsets in Periodontal Health and Disease: A Mini Review

Josefine Hirschfeld
2020 Frontiers in Immunology  
Several studies have investigated different markers of neutrophils in oral health and disease in recent years in order to define potential cell subsets and their specific tasks.  ...  The aim of this review is to give an overview of the current evidence regarding the existence and presence of neutrophil subsets and their possible functions, specifically in the context of periodontitis  ...  (d) the formation of neutrophil extracellular traps (NETs), by which neutrophils release decondensed chromatin decorated with histones and granule contents into the extracellular space where they trap  ... 
doi:10.3389/fimmu.2019.03001 pmid:31998301 pmcid:PMC6961529 fatcat:mbnnropfezgl5awpo33kyaku34

Neutrophil Extracellular Traps Induce MCP-1 at the Culprit Site in ST-Segment Elevation Myocardial Infarction

Thomas M. Hofbauer, Anna S. Ondracek, Andreas Mangold, Thomas Scherz, Johanna Nechvile, Veronika Seidl, Christine Brostjan, Irene M. Lang
2020 Frontiers in Cell and Developmental Biology  
CCR2 expression of culprit site and peripheral blood fibrocytes was characterized by flow cytometry.  ...  We recently observed that neutrophil extracellular traps (NETs) are increased at the culprit site, promoting activation and differentiation of fibrocytes, cells with mesenchymal and leukocytic properties  ...  CCR2 expression of culprit site and peripheral blood fibrocytes was characterized by flow cytometry.  ... 
doi:10.3389/fcell.2020.564169 pmid:33240874 pmcid:PMC7680894 fatcat:23ttg2gsyfd2xenbvqultgtop4

Neutrophils Induce a Novel Chemokine Receptors Repertoire During Influenza Pneumonia

Jennifer M. Rudd, Sivasami Pulavendran, Harshini K. Ashar, Jerry W. Ritchey, Timothy A. Snider, Jerry R. Malayer, Montelongo Marie, Vincent T. K. Chow, Teluguakula Narasaraju
2019 Frontiers in Cellular and Infection Microbiology  
We have previously demonstrated that excessive neutrophils recruited during influenza infection drive pulmonary pathology through induction of neutrophil extracellular traps (NETs) and release of extracellular  ...  Here, we examined CC and CXC CRs detection in circulating as well as lung-recruited neutrophils during influenza infection in mice using flow cytometry analyses.  ...  Flow Cytometry Analyses The following mouse antibodies were purchased from R&D Systems and used for flow cytometry for detection of chemokine receptors in neutrophils: CCR1 FITC-conjugated antibody (Clone  ... 
doi:10.3389/fcimb.2019.00108 pmid:31041196 pmcid:PMC6476945 fatcat:jrj7a34hw5enpiroyajjz32gaq

Neutrophil Extracellular Traps Drive Endothelial-to-Mesenchymal TransitionHighlights

Elmar Pieterse, Nils Rother, Marjolein Garsen, Julia M. Hofstra, Simon C. Satchell, Markus Hoffmann, Markus A. Loeven, Hanneke K. Knaapen, Olivier W.H. van der Heijden, Jo H.M. Berden, Luuk B. Hilbrands, Johan van der Vlag
2017 Arteriosclerosis, Thrombosis and Vascular Biology  
Sources of Funding Research was supported by the Dutch Kidney Foundation (KSBS 12.073), Department of Nephrology Radboudumc, the RIMLS Radboudumc PhD program and the Radboudumc Honours Academy.  ...  Acknowledgments We thank the Dutch Working Party on Systemic Lupus Erythematosus for the collection of human material.  ...  Figure 3 . 3 Neutrophil extracellular traps (NETs) activate β-catenin signaling and promote endothelial-to-mesenchymal transition (EndMT).  ... 
doi:10.1161/atvbaha.117.309002 pmid:28495931 fatcat:hniluv6qkvdw3ltpmgcshouzhe

Candida albicans-Induced NETosis Is Independent of Peptidylarginine Deiminase 4

Eva Guiducci, Christina Lemberg, Noëmi Küng, Elisabeth Schraner, Alexandre P. A. Theocharides, Salomé LeibundGut-Landmann
2018 Frontiers in Immunology  
Among the neutrophils' arsenal of effector functions, neutrophil extracellular traps (NETs) are thought to be of particular importance for trapping and killing the large fungal filaments by means of their  ...  Peptidylarginine deiminase 4 (PAD4)-mediated citrullination of histones in activated neutrophils correlates with chromatin decondensation and extrusion and is widely accepted to act as an integral process  ...  and Image Analysis of the University of Zürich for scanning electron microscopy; Annette Oxenius, Onur Boyman, and all members of the LeibundGut-lab for helpful advice and discussions.  ... 
doi:10.3389/fimmu.2018.01573 pmid:30038623 pmcid:PMC6046457 fatcat:hulfnlflzjhm5dx7vbnhwahvqu
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