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Identification Methods of G Protein-Coupled Receptors

Meriem Zekri, Karima Alem, Labiba Souici-Meslati
2011 International Journal of Knowledge Discovery in Bioinformatics  
Identification Methods of G Protein-Coupled Receptors ABSTRACT The G protein-coupled receptors (GPCRs) include one of the largest and most important families of multifunctional proteins known to molecular  ...  A receptor is thus a protein having ligand to an informational molecule from the external environment. One of the largest families of receptors is formed by G protein-coupled receptors (GPCRs).  ... 
doi:10.4018/jkdb.2011100103 fatcat:7yk6j4a4jvfrvb6hpxpzjuv6xi

Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors [chapter]

Guangyu Wu
2013 Methods in Enzymology  
These studies indicate that ER export of nascent G protein-coupled receptors (GPCRs) may be directed by specific codes that mediate receptor interaction with the ER-derived COPII vesicles.  ...  Moreover, ER export motifs are able to confer their transport abilities to other proteins. We have recently identified a novel ER export motif for α(2B)-adrenergic receptor (α(2B)-AR).  ...  Acknowledgments This work was supported by National Institutes of Health Grant R01GM076167.  ... 
doi:10.1016/b978-0-12-391862-8.00010-7 pmid:23351740 pmcid:PMC3611588 fatcat:lpzkkfs3mbfwjhug7evcbow464

Heteromerization of G-Protein–Coupled Receptors. Implications for Central Nervous System Function and Dysfunction

Sergi Ferré
2007 The Scientific World Journal  
The biochemical fingerprint of the dopamine D 1 -D 2 receptor heteromer, reviewed by George and O'Dowd[6], is its characteristic G-protein coupling (G q/11 ), which leads to a specific calcium-dependent  ...  Like many new concepts, especially those that break old dogmas, it took some time for the scientific community to accept the existence and functional significance of G-protein-coupled receptor (GPCR) heteromerization  ...  The biochemical fingerprint of the dopamine D 1 -D 2 receptor heteromer, reviewed by George and O'Dowd [6] , is its characteristic G-protein coupling (G q/11 ), which leads to a specific calcium-dependent  ... 
doi:10.1100/tsw.2007.215 pmid:17982575 pmcid:PMC5901262 fatcat:ivih54ptmjh4di7gor3ljonfpe

THEIDENTIFICATION OFLIGANDS ATORPHANG-PROTEINCOUPLEDRECEPTORS

Alan Wise, Steven C. Jupe, Stephen Rees
2004 Annual Review of Pharmacology and Toxicology  
Key Words orphan, GPCR, 7-transmembrane receptor s Abstract The completion of the human genome sequencing project has identified approximately 720 genes that belong to the G-protein coupled receptor (GPCR  ...  The identification of ligands active at orphan GPCRs has been achieved through the development of a number of experimental approaches, including the screening of putative small molecule and peptide ligands  ...  in many of the successful orphan GPCR:ligand pairings outlined in this review.  ... 
doi:10.1146/annurev.pharmtox.44.101802.121419 pmid:14744238 fatcat:fh365keu5bf4tfj5x344s2gjqm

Identification of G protein-coupled receptors by RNase H-mediated hybrid depletion usingXenopus laevisoocytes as expression system

Wolfgang Meyerhof, Dietmar Richter
1990 FEBS Letters  
A method has been developed for rapidly identifying putative G protein-coupled receptors isolated initially as small cDNA fragments, following reverse transcription and polymerase chain reaction (PCR)  ...  The method should allow the identification of receptors which can functionally be expressed and measured in Xenopus oocytes.  ...  Acknowledgements: The authors wish to thank H. and G. Ellinghausen for expert technical assistance and Dr S.D. Morley for critical discussion of the manuscript.  ... 
doi:10.1016/0014-5793(90)81537-x pmid:1694786 fatcat:ep5ltxio65hdteggxweewn52dy

Selectivity determinants of GPCR–G-protein binding

Tilman Flock, Alexander S. Hauser, Nadia Lund, David E. Gloriam, Santhanam Balaji, M. Madan Babu
2017 Nature  
The selective coupling of G protein-coupled receptors (GPCRs) to specific G proteins is critical to trigger the appropriate physiological response.  ...  These findings lay the foundation for understanding the molecular basis of coupling selectivity within individual receptors and G proteins.  ...  Harbrecht, G. De Baets, D. Prado, X. Deupi, C. G. Tate and N. S. Latysheva for their comments on this work, and Joanna Westmoreland for assistance with Figure 6 . We thank S.  ... 
doi:10.1038/nature22070 pmid:28489817 pmcid:PMC5846738 fatcat:75f4ctdpjjauriyocyswvrplpi

Identification of G-Protein Coupled Receptor Subunits in Normal Human Dental Pulp

D POZO, J SEGURA, A JIMENEZRUBIO, A GARCIAPERGANEDA, I BETTAHI, J GUERRERO, J CALVO
2000 Journal of Endodontics  
FIG 1 . 1 Schematic representation of the G-protein coupled receptor signaling mechanism of transmembrane communication.  ...  G-protein coupled receptor signaling is the mechanism of transmembrane communication used by these neuropeptides.  ... 
doi:10.1097/00004770-200001000-00004 pmid:11194358 fatcat:mfkgbozhqjfxxizjpwox3ryfy4

Prediction of Drugs Target Groups Based on ChEBI Ontology

Yu-Fei Gao, Lei Chen, Guo-Hua Huang, Tao Zhang, Kai-Yan Feng, Hai-Peng Li, Yang Jiang
2013 BioMed Research International  
Thus, knowledge of drugs target proteins is essential for the improvement of therapeutic effects and mitigation of undesirable side effects.  ...  Most drugs have beneficial as well as adverse effects and exert their biological functions by adjusting and altering the functions of their target proteins.  ...  Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper. Acknowledgments  ... 
doi:10.1155/2013/132724 pmid:24350241 pmcid:PMC3853244 fatcat:mojjpp624nawxlfpmwlyuvmxny

The identification of high-affinity G protein-coupled receptor ligands from large combinatorial libraries using multicolor quantum dot-labeled cell-based screening

Junjie Fu, Timothy Lee, Xin Qi
2014 Future Medicinal Chemistry  
G protein-coupled receptors (GPCRs), which are involved in virtually every biological process, constitute the largest family of transmembrane receptors.  ...  G protein-coupled receptors as drug targets G protein-coupled receptors (GPCRs) are characterized by an extracellular N-terminus and an intracellular C-terminus connected by seven transmembrane α-helical  ...  Executive summary G protein-coupled receptors as drug targets • G protein-coupled receptors (GPCRs) are the largest and the most important classes of proteins due to their critical role in cell signaling  ... 
doi:10.4155/fmc.14.38 pmid:24941874 pmcid:PMC4172322 fatcat:cupxcoh3xzbkje2aiqw5uppwmi

Page 152 of Cellular and Molecular Life Sciences Vol. 62, Issue 5 [page]

2005 Cellular and Molecular Life Sciences  
J. and Colucci G. (2003) The G-protein-coupled receptor GCRI regulates DNA synthesis through activation of phos- phatidylinositol-specific phospholipase C.  ...  M., Davignon I., Yu K et al. (1999) RGS proteins determine signaling specificity of Gq-coupled receptors. J. Biol.  ... 

Prediction of the coupling specificity of G protein coupled receptors to their G proteins

S. Moller, J. Vilo, M. D.R. Croning
2001 Bioinformatics  
G protein coupled receptors (GPCRs) are found in great numbers in most eukaryotic genomes.  ...  for coupling to a particular functional class of G proteins.  ...  ACKNOWLEDGEMENTS The authors wish to thank Anders Krogh and Henrik Nielsen for the collaborative development of 7TMHMM.  ... 
doi:10.1093/bioinformatics/17.suppl_1.s174 pmid:11473007 fatcat:37mtgcgtefewra4asg67bxtibm

Old concepts, new molecules and current approaches applied to the bacterial nucleotide signalling field

Angelika Gründling, Vincent T. Lee
2016 Philosophical Transactions of the Royal Society of London. Biological Sciences  
One contribution of 15 to a discussion meeting issue 'The new bacteriology'.  ...  identification of new receptors [57] .  ...  The above-mentioned approach was improved using modified cyclic dinucleotides coupled to affinity resin, which allowed for the purification of receptor proteins followed by their identification through  ... 
doi:10.1098/rstb.2015.0503 pmid:27672152 pmcid:PMC5052745 fatcat:zd3eeck4a5br5dpbfuqoatu2ii

Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

Timothy E Gookin, Junhyong Kim, Sarah M Assmann
2008 Genome Biology  
The heterotrimeric G-protein signaling mechanism is conserved across metazoa, and also readily identifiable in plants, but the low sequence conservation of G-protein coupled receptors hampers the identification  ...  The classic paradigm of heterotrimeric G-protein signaling describes a heptahelical, membrane-spanning G-protein coupled receptor that physically interacts with an intracellular Gα subunit of the G-protein  ...  The authors appreciate the input from Dr Sona Pandey and Ms Liza Wilson concerning an early version of the Arabidopsis QFC dataset, and the timely and excellent technical assistance of Ms Stephanie Gookin  ... 
doi:10.1186/gb-2008-9-7-r120 pmid:18671868 pmcid:PMC2530877 fatcat:hstv27in5rflxlwf3qlgbh5bye

Prediction of G Protein Coupled Receptors from Plant Genomes

Deisy Xavier Amora, Emmanuel Bresso, Roberto Coiti Togawa, Priscila Grynberg, Bernard Maigret, tália Florêncio Martins
2016 International Journal of Current Research in Biosciences and Plant Biology  
G-protein coupled receptors (GPCRs) are the largest family of membrane proteins, as key components of signal transduction pathways.  ...  From a total of 997,435 proteins collected, the sequences were selected by length, reducing the initial collection to 487,012.  ...  GCR1, cAMP receptor -G protein-coupled receptor 1 cAMP receptor; GPR108 -G protein-coupled receptor 108 and TMEM87b-Transmembrane protein 87B.  ... 
doi:10.20546/ijcrbp.2016.311.014 fatcat:ru7w24wvizg2jllciheuomfseq

DREADDs: novel tools for drug discovery and development

Hyeong-Min Lee, Patrick M. Giguere, Bryan L. Roth
2014 Drug Discovery Today  
Since the invention of the first designer receptors exclusively activated by designer drugs (DREADDs), these engineered G protein-coupled receptors (GPCRs) have been widely applied in investigations of  ...  DREADDs can facilitate the identification of druggable targets and enable researchers to explore the activities of novel drugs against both known and orphan GPCRs.  ...  G protein-coupled receptors (GPCRs) are activated by a variety of external stimuli. Upon receptor activation, the G protein exchanges GDP for GTP, causing the dissociation of the GTP-bound ! and !/!  ... 
doi:10.1016/j.drudis.2013.10.018 pmid:24184433 pmcid:PMC4004703 fatcat:jsofekpezbdhtctkb3savhdome
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