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Chemotaxis model for human breast cancer cells based on signal-to-noise ratio
[article]
2018
bioRxiv
pre-print
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Chemotaxis, a biased migration of cells under a chemical gradient, plays a significant role in diverse biological phenomena including cancer metastasis. Stromal cells release signaling proteins to induce chemotaxis, which further causes organ-specific metastasis. Epidermal growth factor (EGF) is an example of the chemical attractants, and its gradient stimulates metastasis of breast cancer cells. Hence, the interactions between EGF and breast cancer cells have long been a subject of interest
doi:10.1101/292300
fatcat:mtjxv7eejbbhpl3miuwh3gxu5i
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... oncologists and clinicians. However, most current approaches do not systematically separate the effects of gradient and absolute concentration of EGF on chemotaxis of breast cancer cells. In this work, we develop a theoretical model based on signal-to-noise ratio to represent stochastic properties and report our microfluidic experiments to verify the analytical predictions from the model. The results demonstrate that even under the same EGF concentration gradients, breast cancer cells can reveal distinct chemotaxis patterns at different absolute concentrations. Moreover, we found that addition of EGF receptor antibody can promote chemotaxis at a low EGF level. This apparently counterintuitive finding suggests that EGF receptor-targeted therapy may stimulate metastasis of breast cancer at a particular condition, which should be considered in anticancer drug design.
Chemotaxis Model for Breast Cancer Cells Based on Signal/Noise Ratio
2018
Biophysical Journal
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A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
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Chemotaxis, a biased migration of cells under a chemical gradient, plays a significant role in diverse biological phenomena including cancer metastasis. Stromal cells release signaling proteins to induce chemotaxis, which leads to organ-specific metastasis. Epidermal growth factor (EGF) is an example of the chemical attractants, and its gradient stimulates metastasis of breast cancer cells. Hence, the interactions between EGF and breast cancer cells have long been a subject of interest for
doi:10.1016/j.bpj.2018.09.028
pmid:30366624
pmcid:PMC6303234
fatcat:zd5usjozsbed7p3ndx5byh3c2y
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... ogists and clinicians. However, most current approaches do not systematically separate the effects of gradient and absolute concentration of EGF on chemotaxis of breast cancer cells. In this work, we develop a theoretical model based on signal/noise ratio to represent stochastic properties and report our microfluidic experiments to verify the analytical predictions from the model. The results demonstrate that even under the same EGF concentration gradients (0-50 or 0-150 ng/mL), breast cancer cells reveal a more evident chemotaxis pattern when the absolute EGF concentrations are low. Moreover, we found that reducing the number of EGF receptors (EGFRs) with addition of EGFR antibody (1 ng/mL) can promote chemotaxis at an EGF gradient of 0-1 ng/mL as shown by chemotaxis index (0.121 ± 0.037, reduced EGFRs vs. 0.003 ± 0.041, control). This counterintuitive finding suggests that EGFR-targeted therapy may stimulate metastasis of breast cancer because the partial suppression of the receptors makes the number of receptors close to the optimal one for chemotaxis. This analysis should be considered in anticancer drug design.
Reagent- and actuator-free analysis of individual erythrocytes using three-dimensional quantitative phase imaging and capillary microfluidics
[article]
2021
bioRxiv
pre-print
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Histopathological examination of blood cells plays a crucial role in the diagnosis of various diseases. However, it involves time-consuming and laborious staining procedures required for microscopic review by medical experts and is not directly applicable for point-of-care diagnosis in resource-limited locations. This study reports a dilution-, actuation- and label-free method for the analysis of individual red blood cells (RBCs) using a capillary microfluidic device and quantitative phase
doi:10.1101/2021.05.15.442583
fatcat:okver3jpxjdjlpfi6vz3y3vroi
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... ng. Blood, without any sample treatment, is directly loaded into a micrometer-thick channel such that it forms a quasi-monolayer inside the channel. The morphological and biochemical properties of RBCs, including hemoglobin concentration, hemoglobin content, and corpuscular volume, were retrieved using the refractive index tomograms of individual RBCs measured using 3D quantitative phase imaging. The deformability of individual RBCs was also obtained by measuring the dynamic membrane fluctuations. The proposed framework applies to other imaging modalities and biomedical applications, facilitating rapid and cost-effective diagnosis and prognosis of diseases.
Efficient Capture and Raman Analysis of Circulating Tumor Cells by Nano-Undulated AgNPs-rGO Composite SERS Substrates
2020
Sensors
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The analysis of circulating tumor cells (CTCs) in the peripheral blood of cancer patients is critical in clinical research for further investigation of tumor progression and metastasis. In this study, we present a novel surface-enhanced Raman scattering (SERS) substrate for the efficient capture and characterization of cancer cells using silver nanoparticles-reduced graphene oxide (AgNPs-rGO) composites. A pulsed laser reduction of silver nanowire-graphene oxide (AgNW-GO) mixture films induces
doi:10.3390/s20185089
pmid:32906807
fatcat:htuwk3hrtrbyhdzd3z63pg27ea
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... ot-spot formations among AgNPs and artificial biointerfaces consisting of rGOs. We also use in situ electric field-assisted fabrication methods to enhance the roughness of the SERS substrate. The AgNW-GO mixture films, well suited for the proposed process due to its inherent electrophoretic motion, is adjusted between indium tin oxide (ITO) transparent electrodes and the nano-undulated surface is generated by applying direct-current (DC) electric fields during the laser process. As a result, MCF7 breast cancer cells are efficiently captured on the AgNPs-rGO substrates, about four times higher than the AgNWs-GO films, and the captured living cells are successfully analyzed by SERS spectroscopy. Our newly designed bifunctional substrate can be applied as an effective system for the capture and characterization of CTCs.
Recycling silver nanoparticle debris from laser ablation of silver nanowire in liquid media toward minimum material waste
2021
Scientific Reports
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AbstractAs silver nanowires (Ag NWs) are usually manufactured by chemical synthesis, a patterning process is needed to use them as functional devices. Pulsed laser ablation is a promising Ag NW patterning process because it is a simple and inexpensive procedure. However, this process has a disadvantage in that target materials are wasted owing to the subtractive nature of the process involving the removal of unnecessary materials, and large quantities of raw materials are required. In this
doi:10.1038/s41598-021-81692-9
pmid:33500481
fatcat:nwuptpb42zhlba3trvlizvalpy
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... , we report a minimum-waste laser patterning process utilizing silver nanoparticle (Ag NP) debris obtained through laser ablation of Ag NWs in liquid media. Since the generated Ag NPs can be used for several applications, wastage of Ag NWs, which is inevitable in conventional laser patterning processes, is dramatically reduced. In addition, electrophoretic deposition of the recycled Ag NPs onto non-ablated Ag NWs allows easy fabrication of junction-enhanced Ag NWs from the deposited Ag NPs. The unique advantage of this method lies in using recycled Ag NPs as building materials, eliminating the additional cost of junction welding Ag NWs. These fabricated Ag NW substrates could be utilized as transparent heaters and stretchable TCEs, thereby validating the effectiveness of the proposed process.
Table of Contents
2019
2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)
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High-Throughput Microfluidic 3D Cytotoxicity Assay for Cancer Immunotherapy (CACI-IMPACT
Platform)
129
Yunchan Hwang
Directional Migration of Cancer Cells Under Oxygen Tension Gradient
130
Hyeono ...
Lai
Peptide-based Immunological Cloaking for Efficient In vivo Phage Therapy
78
Yoon Sung Nam
Multi-functional Hydrogel-incorporated Nanofiber Scaffolds Capable of Controlled Release of
Growth ...
doi:10.1109/nanomed49242.2019.9130620
fatcat:xgquijr23rf5dlm354zctinr4a
Kimdaejung Convention Center
2019
2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)
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Speaker Bio-Hyeono Nam is a Ph.D. candidate in the department of mechanical engineering at KAIST. ...
SS7 Smart Biomaterials for Bio/Nano-Applications Speaker Bio-Yoon Sung Nam is an associate professor of Materials Science and Engineering at KAIST. ...
doi:10.1109/nanomed49242.2019.9130603
fatcat:wlmmq4uwyjablibepjnazw4fp4