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Improvement of Bacterial Vaginosis by Oral Lactobacillus Supplement: A Randomized, Double-Blinded Trial

Ta-Chin Lin, I-Ling Hsu, Wan-Hua Tsai, Yi-Chih Chu, Lung-Ching Kuan, Min-Syuan Huang, Wen-Ling Yeh, Ya-Hui Chen, Shan-Ju Hsu, Wen-Wei Chang
2021 Applied Sciences  
Bacterial vaginosis (BV) is the most common vaginal infection globally, with a high recurrent rate after antibiotic treatment. Probiotics consumption is known to improve BV with different efficacy among species or strains. After in vitro selection of Lactobacillus strains with growth inhibition and preventing adhesion to HeLa cervical epithelial cells, a randomized and double-blinded trial of two Lactobacillus formula, namely, VGA-1 and VGA-2, in BV patients with Nugent scores of 4–10 was
more » ... ted. Among 37 subjects who completed the trial, we observed significantly decreased Nugent scores in both VGA-1 (n = 18) and VGA-2 (n = 19) consumption groups. VGA-1 consumption significantly improved vaginal discharge odor/color and itching at both 2-week and 4-week-consumption, but those only observed after a 4-week-consumption in the VGA-2 group. We also observed a tendency to reduce recurrent rates among enrolled participants after VGA-1 or VGA-2 consumption. The improvement effect of VGA-1/VGA-2 was associated with the significant reduction of interleukin-6 expression after 4-week-consumption and the restoration of normal vaginal microflora by quantitative polymerase chain reaction analysis. In conclusion, VGA-1 or VGA-2 displayed beneficial effects in BV patients, but the VGA-1 formula showed a better efficacy, potentially used for BV intervention.
doi:10.3390/app11030902 fatcat:x63idt6l3zhpvn2o4cbrhaz46u

Primary breast lymphoma

En-Hua Huang, Sheng-Fung Lin, Hsin-Ling Yin, Jui-Sheng Hsu
2013 Kaohsiung Journal of Medical Sciences  
doi:10.1016/j.kjms.2012.12.008 pmid:23906238 fatcat:tmktwfg65nezhbuuusz5cimb6q

Strong edge-coloring for jellyfish graphs

Gerard J. Chang, Sheng-Hua Chen, Chi-Yun Hsu, Chia-Man Hung, Huei-Ling Lai
2015 Discrete Mathematics  
A strong edge-coloring of a graph is a function that assigns to each edge a color such that two edges within distance two apart receive different colors. The strong chromatic index of a graph is the minimum number of colors used in a strong edge-coloring. This paper determines strong chromatic indices of cacti, which are graphs whose blocks are cycles or complete graphs of two vertices. The proof is by means of jellyfish graphs.
doi:10.1016/j.disc.2015.04.031 fatcat:oblyvuckbjdzxgmspuuafcp6la

How Does C-V2X Help Autonomous Driving to Avoid Accidents?

Lili Miao, Shang-Fu Chen, Yu-Ling Hsu, Kai-Lung Hua
2022 Sensors  
Accidents are continuously reported for autonomous driving vehicles including those with advanced sensors installed. Some of accidents are usually caused by bad weather, poor lighting conditions and non-line-of-sight obstacles. Cellular Vehicle-to-Everything (C-V2X) radio technology can significantly improve those weak spots for autonomous driving. This paper describes one of the C-V2X system solutions: Vulnerable Road User Collision Warning (VRUCW) for autonomous driving. The paper provides
more » ... system architecture, design logic, network topology, message flow, artificial intelligence (AI) and network security feature. As a reference it also includes a commercial project with its test results.
doi:10.3390/s22020686 pmid:35062647 pmcid:PMC8779724 fatcat:fdho6yzudnasfmsilxv625rww4

Can Higher Education, Economic Growth and Innovation Ability Improve Each Other?

Haiying Xu, Wei-Ling Hsu, Teen-Hang Meen, Ju Hua Zhu
2020 Sustainability  
This study argues that the coupling between higher education, economic growth, and innovation ability is of great significance for regional sustainable development. Through the experience of Jiangsu Province in China, this study establishes a coupling coordination evaluation index system and applies the coupling coordination model to evaluate interactive relationships among the three. It finds that during 2007–2017, the level of coupling of 13 prefecture-level cities in Jiangsu was increasing
more » ... er time, which fully verified the previous scholars' view that the three can improve each other over a long period. However, this study finds that there are obvious differences within Jiangsu. Inadequate investment in higher education has become a crucial constraint on sustainable economic growth in northern and central Jiangsu, which are backward regions of Jiangsu. By contrast, in southern Jiangsu, which is the advanced region of Jiangsu, although the resources of higher education are abundant the growth of innovation ability cannot support sustained economic growth well. Thus, the quality of higher education should be improved to meet the needs of the innovation-based economy. Accordingly, cross-regional cooperation and balanced investment in higher education are the keys to practicing a balanced and sustained regional development. The results of this study's coupling coordination analysis and evaluation can serve as a reference for governments in enhancing regional sustainable development.
doi:10.3390/su12062515 fatcat:hvi26ms3xfhhlovggjkobd2j3a

A fusiform nodule on the flank

Lu-An Chen, Ying-Jui Chang, Lin-Hui Su, Yu-Ling Hsu, Tsung-Hua Tsai
2010 Zhōnghuá Pífūkē Yīxué Zázhì  
An otherwise healthy 61-year-old female presented with a skin tumor on the left flank, which had been present since her early adulthood. The tumor gradually enlarged during the first 2-3 years and then stabilized in size. There were no symptoms from the tumor. On examination, a red to violaceous, shiny, polypoid, fusiform nodule with firm consis tency, measuring approximately 3.0 cm × 2.0 cm, was noted on her left flank ( Figure 1 ). There were also many skin tumors on her face and scalp, most
more » ... f which were dome-shaped, smooth surfaced, violaceous, firm nodules measuring several millimeters in diameter. These tumors were 1-cm dermal nodules that caused pain; others were rounded, skin-colored, firm papules with a size of approximately 5 mm in diameter around her nose. An excisional biopsy specimen was obtained from the tumor at the flank. Histopathologic examination revealed a tumor occupying the reticular dermis, which was composed of aggregations of neoplastic cells arranged in a jigsaw puzzle pattern. The aggregations consisted of neoplastic cells with two different appearances: (1) peripheral cells aligned in a palisade with small, dark staining columnar nuclei surrounded by a scant cytoplasm, and (2) cells with nuclei that were larger and paler, and that had a more abundant pale cytoplasm. Each aggregation was surrounded by a rim of homogenous eosinophilic basement membrane-like material. Globules of the same material were also present within the aggregations themselves ( Figure 2) . The patient then underwent several sessions of surgical removal for other tumors on the face and scalp, and pathology exams showed cylindroma, spiradenoma, trichoepithelioma, or combinations of them ( Figure 3A : cylindroma + trichoepithelioma; Figure 3B : cylindroma + spiradenoma). She reported that two of her younger brothers and sisters also had similar skin tumors, but they were not as numerous as hers. Figure 1 A fusiform nodule on the patient's left flank. Figure 2 Peripheral columnar cells and central cells with abundant cytoplasms (H&E, 400×).
doi:10.1016/s1027-8117(10)60029-8 fatcat:eceyhfuh5bh4rdeyvtawfux5ky

Solvent Effects on Morphology and Electrical Properties of Poly(3-hexylthiophene) Electrospun Nanofibers

Jung-Yao Chen, Chien-You Su, Chau-Hsien Hsu, Yi-Hua Zhang, Qin-Cheng Zhang, Chia-Ling Chang, Chi-Chung Hua, Wen-Chang Chen
2019 Polymers  
Herein, poly(3-hexylthiophene-2,5-diyl) (P3HT) nanofiber-based organic field-effect transistors were successfully prepared by coaxial electrospinning technique with P3HT as the core polymer and poly(methyl methacrylate) (PMMA) as the shell polymer, followed by extraction of PMMA. Three different solvents for the core polymer, including chloroform, chlorobenzene and 1,2,4-trichlorobenzene, were employed to manipulate the morphologies and electrical properties of P3HT electrospun nanofibers.
more » ... gh the analyses from dynamic light scattering of P3HT solutions, polarized photoluminescence and X-ray diffraction pattern of P3HT electrospun nanofibers, it is revealed that the P3HT electrospun nanofiber prepared from the chloroform system displays a low crystallinity but highly oriented crystalline grains due to the dominant population of isolated-chain species in solution that greatly facilitates P3HT chain stretching during electrospinning. The resulting high charge-carrier mobility of 3.57 × 10−1 cm2·V−1·s−1 and decent mechanical deformation up to a strain of 80% make the P3HT electrospun nanofiber a promising means for fabricating stretchable optoelectronic devices.
doi:10.3390/polym11091501 pmid:31540102 pmcid:PMC6780587 fatcat:346br7o27jhg3ekdd6gp2apcha

Comparing the burdens of opportunistic infections among patients with systemic rheumatic diseases: a nationally representative cohort study

Chung-Yuan Hsu, Chi-Hua Ko, Jiun-Ling Wang, Tsai-Ching Hsu, Chun-Yu Lin
2019 Arthritis Research & Therapy  
To estimate and compare the burdens of opportunistic infections and herpes zoster in real-world practice among patients with various systemic rheumatic diseases.
doi:10.1186/s13075-019-1997-5 pmid:31604447 pmcid:PMC6790041 fatcat:a3i63ga275gozdvacy2edvsapu

Enhancement of Osteoblast Function through Extracellular Vesicles Derived from Adipose-Derived Stem Cells

Mei-Ling Ho, Chin-Jung Hsu, Che-Wei Wu, Ling-Hua Chang, Jhen-Wei Chen, Chung-Hwan Chen, Kui-Chou Huang, Je-Ken Chang, Shun-Cheng Wu, Pei-Lin Shao
2022 Biomedicines  
Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell that is investigated in bone tissue engineering (BTE). Osteoblasts are the main cells responsible for bone formation in vivo and directing ADSCs to form osteoblasts through osteogenesis is a research topic in BTE. In addition to the osteogenesis of ADSCs into osteoblasts, the crosstalk of ADSCs with osteoblasts through the secretion of extracellular vesicles (EVs) may also contribute to bone formation in ADSC-based BTE. We
more » ... vestigated the effect of ADSC-secreted EVs (ADSC-EVs) on osteoblast function. ADSC-EVs (size ≤ 1000 nm) were isolated from the culture supernatant of ADSCs through ultracentrifugation. The ADSC-EVs were observed to be spherical under a transmission electron microscope. The ADSC-EVs were positive for CD9, CD81, and Alix, but β-actin was not detected. ADSC-EV treatment did not change survival but did increase osteoblast proliferation and activity. The 48 most abundant known microRNAs (miRNAs) identified within the ADSC-EVs were selected and then subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The GO analysis revealed that these miRNAs are highly relevant to skeletal system morphogenesis and bone development. The KEGG analysis indicated that these miRNAs may regulate osteoblast function through autophagy or the mitogen-activated protein kinase or Ras-related protein 1 signaling pathway. These results suggest that ADSC-EVs enhance osteoblast function and can contribute to bone regeneration in ADSC-based BTE.
doi:10.3390/biomedicines10071752 pmid:35885057 pmcid:PMC9312889 fatcat:thm3srblcvfqnjgwsdgrb4lere

Using Anti-Malondialdehyde Modified Peptide Autoantibodies to Import Machine Learning for Predicting Coronary Artery Stenosis in Taiwanese Patients with Coronary Artery Disease

Yu-Cheng Hsu, I-Jung Tsai, Hung Hsu, Po-Wen Hsu, Ming-Hui Cheng, Ying-Li Huang, Jin-Hua Chen, Meng-Huan Lei, Ching-Yu Ling
2021 Diagnostics  
female and 124 male patients, aged 62.99 ± 9.59 years) and 40 HCs (16 females and 24 males, aged 38.41 ± 10.42 years) were obtained from the Cardiovascular Center and Department of Laboratory Medicine, Lo-Hsu  ... 
doi:10.3390/diagnostics11060961 pmid:34073646 fatcat:5b4dfnr77fbcro34zdhz2q634e

Phosphodiesterase Type 5 (PDE5) Inhibitors Sensitize Topoisomerase II Inhibitors in Killing Prostate Cancer Through PDE5-Independent Impairment of HR and NHEJ DNA Repair Systems

Jo-Fan Chang, Jui-Ling Hsu, Yi-Hua Sheng, Wohn-Jenn Leu, Chia-Chun Yu, She-Hung Chan, Mei-Ling Chan, Lih-Ching Hsu, Shih-Ping Liu, Jih-Hwa Guh
2019 Frontiers in Oncology  
Human castration-resistant prostate cancer (CRPC) is a significant target of clinical research. The use of DNA-damaging agents has a long history in cancer chemotherapy but is limited by their toxicities. The combination with a safer drug can be a strategy in reducing dosage and toxicity while increasing anticancer activity in CRPC treatment. Phosphodiesterase type 5 (PDE5) inhibitors are used to treat erectile dysfunction through the selective inhibition of PDE5 that is responsible for cGMP
more » ... radation in the corpus cavernosum. Several studies have reported that PDE5 inhibitors display protective effect against doxorubicin-induced cardiotoxicity. The combinatory treatment of CRPC with doxorubicin and PDE5 inhibitors has been studied accordingly. The data demonstrated that sildenafil or vardenafil (two structure-related PDE5 inhibitors) but not tadalafil (structure-unrelated to sildenafil) sensitized doxorubicin-induced apoptosis in CRPC cells with deteriorating the down-regulation of anti-apoptotic Bcl-2 family members, including Bcl-xL and Mcl-1, and amplifying caspase activation. Homologous recombination (HR) and non-homologous end joining (NHEJ) DNA repair systems were inhibited in the apoptotic sensitization through detection of nuclear foci formation of Rad51 and DNA end-binding of Ku80. PDE5 knockdown to mimic the exposure to PDE5 inhibitors did not reproduce apoptotic sensitization, suggesting a PDE5-independent mechanism. Not only doxorubicin, sildenafil combined with other inhibitors of topoisomerase II but not topoisomerase I also triggered apoptotic sensitization. In conclusion, the data suggest that sildenafil and vardenafil induce PDE5-independent apoptotic sensitization to doxorubicin (or other topoisomerase II inhibitors) through impairment of both HR and NHEJ repair systems that are evident by a decrease of nuclear Rad51 levels and their foci formation in the nucleus, and an inhibition of Ku80 DNA end-binding capability. The combinatory treatment may enable an important strategy for anti-CRPC development.
doi:10.3389/fonc.2018.00681 pmid:30705876 pmcid:PMC6344441 fatcat:efa54lpvsnhj3lerylp3ipsbcm

Gene Expression Profiles of the Aurora Family Kinases

2006 Gene Expression  
The evolutionarily conserved Aurora family kinases, a family of mitotic serine/threonine kinases, has three members in humans (Aurora-A, -B and -C). Overexpression of Aurora family members, particularly Aurora-A, has been reported in many human cancers and cell lines. In this study, we present evidence based on comparative gene expression analysis via quantitative RT-PCR to delineate the relative contributions of these kinases in 60 cell lines and statistical analysis in five different human
more » ... cer microarray datasets. The analysis demonstrated the selective upregulation of these Aurora members in various cancers. In general, Aurora-A exhibited the highest expression levels, with substantially decreased quantities of the Aurora-C transcript detected relative to Aurora-A and -B. Moreover, to characterize the roles of each Aurora member, which share many similarities, we investigated the expression profiles of the family in normal tissues and a panel of different phases of the HeLa cell cycle. Finally, both Aurora-A and -B were overexpressed in a majority of esophageal tumor tissues in comparison to the normal variants. Taken together, the results show that each Aurora member exhibits distinct expression patterns, implying that they are engaged in different biological processes to accomplish more elaborate cell physiological functions in higher organisms.
doi:10.3727/000000006783991962 pmid:16572587 pmcid:PMC6032448 fatcat:eubdow6xxjdf3esygs4kbdt4u4

Comparison of visual effects of immersion fluids for dermoscopic examination of acral volar melanocytic lesions

Tzu-Hsiu Chen, Shu-Hui Wang, Lin-Hui Su, Yu-Ling Hsu, Tsung-Hua Tsai, Ya-Jing Hsu, Ying-Jui Chang
2014 Zhōnghuá Pífūkē Yīxué Zázhì  
Keywords: acral volar melanocytic lesion dermoscopy fluid immersion a b s t r a c t Background: Acral volar melanocytic lesions have a high potential for malignancy. Dermoscopy is a useful, noninvasive tool for the diagnosis of such malignancies. The use of immersion fluids can provide better visual effects and improve the diagnostic accuracy of dermoscopic examinations. Methods: Fifteen volar melanocytic lesions, including two palmar and 13 plantar lesions, were included in our study. We
more » ... ed the visual effects of two different immersion fluids as an interface for dermoscopy. Four combinations of immersion fluid (ultrasound jelly or mineral oil) and observation mode (polarized or nonpolarized light) were compared for visual effects during the dermoscopic examination of lesions based on air bubble inclusion and microstructure visibility. Results: Both the mineral oil and the ultrasound jelly allowed at least one microstructure to be clearly visible in each image of the acral volar melanocytic lesions examined. All modes of observation achieved acceptable visual effects. The use of mineral oil and the polarized light mode resulted in the formation of fewer bubbles than the use of mineral oil and the nonpolarized light mode (p < 0.05). The use of ultrasound jelly and the polarized light mode resulted in significantly better visual effects (p < 0.05) than that of ultrasound jelly or mineral oil and the nonpolarized light mode. Conclusion: The use of either mineral oil or ultrasound jelly as interface provides acceptable visual effects for the dermoscopic examination of acral volar melanocytic lesions. The use of the polarized light mode reduced the reflection and scattering of light, resulting in better visual effect than that achieved using the nonpolarized light mode. In the early diagnosis of acral melanoma, choosing the appropriate application of immersion fluid and observation mode yields the optimal visual effect.
doi:10.1016/j.dsi.2013.09.014 fatcat:agy3lituifcvxnxewpwi2wo7em

Establishment of Smart Living Environment Control System

Wei-Ling Hsu, Wen-Te Chen, Hui-Hua Kuo, Yan-Chyuan Shiau, Tian-Yow Chern, Shu-Chen Lai, Wen-Hung Fan
2020 Sensors and materials  
doi:10.18494/sam.2020.2581 fatcat:dm2lrlanijccjjewtlbsmuc5ku

Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs

Long-Jyun Su, Meng-Shiue Wu, Yuen Yung Hui, Be-Ming Chang, Lei Pan, Pei-Chen Hsu, Yit-Tsong Chen, Hong-Nerng Ho, Yen-Hua Huang, Thai-Yen Ling, Hsao-Hsun Hsu, Huan-Cheng Chang
2017 Scientific Reports  
DNA extraction and real-time PCR. Lung tissues (25 mg) from xenotransplanted pigs were homogenized in liquid nitrogen. They were treated with the Purelink Genomic DNA Mini kit (Invitrogen) to extract the genomic DNA of the tissues, following the manufacturer's protocol. Real-time PCR assays were performed in a thermal cycler (ABI 7300, Life Technologies), and each measurement consisted of 12.5 μL TaqMan Universal PCR Master Mix (Applied Biosystems) supplemented with the primer (Alu sequence),
more » ... qMan probe, and DNA templates in a final volume of 25 μL. After incubation of the mixtures at 50 °C for 2 min and 95 °C for 10 min, the PCR reactions were carried out for 40 cycles at 95 °C for 15 s and 60 °C for 1 min. The control experiment consisted of pcMSCs containing a total amount of 200 ng human DNA, which served as the basis to calculate the total amounts of human DNA in the pig's lungs. The assays were repeated in triplicate. Exocytosis assays. pcMSCs were seeded in 10 separate dishes, grown to confluence, and labeled with HSA-coated FNDs at 100 μg/mL for 4 h. The FND-labeled pcMSCs were then thoroughly washed by phosphate-buffered saline (PBS) to remove free FNDs and incubated
doi:10.1038/srep45607 pmid:28358111 pmcid:PMC5372358 fatcat:4y454ufo7refnp53qcv65qi4vq
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