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Imaging the Neuroimmune Dynamics Across Space and Time

Micaël Carrier, Marie-Ève Robert, Fernando González Ibáñez, Michèle Desjardins, Marie-Ève Tremblay
2020 Frontiers in Neuroscience  
to increase the spatial and temporal resolution of neuroimmune imaging.  ...  High-resolution electron microscopy with immunostaining and 3D-reconstruction, as well as super-resolution fluorescence microscopy, provided complementary insights by revealing microglial interventions  ...  and multiphoton microscopy.  ... 
doi:10.3389/fnins.2020.00903 pmid:33071723 pmcid:PMC7539119 fatcat:yc2c2wuxprhiti4vlfs7whmgc4

A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience

Elizabeth M. Haynes, Tyler K. Ulland, Kevin W. Eliceiri
2022 Frontiers in Molecular Neuroscience  
This review seeks to synthesize current knowledge of established and emerging non-mammalian model organisms with advances in cellular-resolution in vivo imaging techniques to suggest new approaches to  ...  We will summarize current tools and in vivo imaging approaches at the single cell scale that could help lead to increased consideration of non-mammalian models in neuroscience research.  ...  Cellular and sub-cellular phenomena that require very high temporal and spatial resolution to observe are largely outside the reach of intravital imaging.  ... 
doi:10.3389/fnmol.2022.867010 pmid:35493325 pmcid:PMC9046975 fatcat:f4uufbuojjerdf3m3qygzfqebu

Imaging Myelination In Vivo Using Transparent Animal Models

Jenea M. Bin, David A. Lyons, Bernard Zalc
2016 Brain Plasticity  
We outline common transgenic technologies used to generate zebrafish and Xenopus that express fluorescent reporters, which can be used to image myelination.  ...  As a consequence, in recent years much effort has been invested in the use of smaller, simpler, transparent model organisms to investigate mechanisms of myelination in vivo.  ...  We would also like to thank members of the Lyons lab who contributed to figures: Rafael Almeida provided cartoons and an mbp:eGFP-CAAX image, Jill Williamson provided the single oligodendrocyte image,  ... 
doi:10.3233/bpl-160029 pmid:29765846 pmcid:PMC5928531 fatcat:lp5uz3kzrbhu5oty2cejpaoxqe

Alterations in mGluR5 Expression and Signaling in Lewy Body Disease and in Transgenic Models of Alpha-Synucleinopathy – Implications for Excitotoxicity

Diana L. Price, Edward Rockenstein, Kiren Ubhi, Van Phung, Natalie MacLean-Lewis, David Askay, Anna Cartier, Brian Spencer, Christina Patrick, Paula Desplats, Mark H. Ellisman, Eliezer Masliah (+1 others)
2010 PLoS ONE  
In this context, levels of mGluR5 were analyzed in the brains of PD and DLB human cases and alpha-syn transgenic (tg) mice and compared to age-matched, unimpaired controls, we report a 40% increase in  ...  Dementia with Lewy bodies (DLB) and Parkinson's Disease (PD) are neurodegenerative disorders of the aging population characterized by the abnormal accumulation of alpha-synuclein (alpha-syn).  ...  Sunny Chow for development of data processing plugins for Image J during the course of these studies. Author Contributions  ... 
doi:10.1371/journal.pone.0014020 pmid:21103359 pmcid:PMC2982819 fatcat:xpxgu7nksfd3ln3hc3buwnwmp4

Advances in Light Microscopy for Neuroscience

Brian A. Wilt, Laurie D. Burns, Eric Tatt Wei Ho, Kunal K. Ghosh, Eran A. Mukamel, Mark J. Schnitzer
2009 Annual Review of Neuroscience  
Mouse genetic approaches permit mosaic and inducible fluorescence-labeling strategies, whereas intrinsic contrast mechanisms allow in vivo imaging of animals and humans without use of exogenous markers  ...  Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties.  ...  ACKNOWLEDGMENTS This review greatly benefited from critical feedback on portions of the text and contributions of images generously provided by many neuroscientists and microscopists.  ... 
doi:10.1146/annurev.neuro.051508.135540 pmid:19555292 pmcid:PMC2820375 fatcat:iihdajrrmramtibudjt66tvroe


2017 The Journal of General Physiology  
Methods: We used the Cre-loxP technique to express GCaMP3 in combination with confocal imaging. Electrical fi eld stimulation (EFS) was used to activate enteric motor neurons.  ...  However, using multi-color super-resolution stochastic optical reconstruction microscopy (STORM), we observed intimate association of individual molecules within signaling complexes containing ion channels  ...  Of the various approaches, multiphoton microscopy (MPM) is particularly well suited for deep, in vivo imaging due to its non-invasiveness and ability to maintain high spatial resolution at depth through  ... 
doi:10.1085/jgp.149.9.889 fatcat:nplsscsocfcpdntcc7wfv6pn4i

Erratum: Visualization of image data from cells to organisms

Thomas Walter, David W Shattuck, Richard Baldock, Mark E Bastin, Anne E Carpenter, Suzanne Duce, Jan Ellenberg, Adam Fraser, Nicholas Hamilton, Steve Pieper, Mark A Ragan, Jurgen E Schneider (+2 others)
2010 Nature Methods  
systematic image data characterizing genes and proteins on a large scale.  ...  Second, the availability of highthroughput techniques enables experiments on a large scale, generating large sets of image  ...  Two-photon microscopy is similar to confocal scanning microscopy but uses nonlinear excitation involving two-photon (or multiphoton) absorption 86 .  ... 
doi:10.1038/nmeth0610-479a fatcat:mimxo5gqnrf6zpu2uhfh7iinxm

Biomechanical coupling facilitates spinal neural tube closure in mouse embryos

Gabriel L. Galea, Young-June Cho, Gauden Galea, Matteo A. Molè, Ana Rolo, Dawn Savery, Dale Moulding, Lucy H. Culshaw, Evanthia Nikolopoulou, Nicholas D. E. Greene, Andrew J. Copp
2017 Proceedings of the National Academy of Sciences of the United States of America  
The authors alone are responsible for the content of this paper, which does not necessarily represent the decisions, policy, or views of the World Health Organization or its member states.  ...  Failure of this closure process leads to open spina bifida, a common cause of severe neurologic disability in humans.  ...  Stained embryos were imaged on a Zeiss Examiner LSM880 confocal microscope. The high-resolution images shown in SI Appendix, section 1, Fig.  ... 
doi:10.1073/pnas.1700934114 pmid:28607062 pmcid:PMC5495245 fatcat:joiexm5iybc4bhfxn3ch4elo5e

The AppNL-G-F mouse retina is a site for preclinical Alzheimer's disease diagnosis and research

Marjan Vandenabeele, Lien Veys, Sophie Lemmens, Xavier Hadoux, Géraldine Gelders, Luca Masin, Lutgarde Serneels, Jan Theunis, Takashi Saito, Takaomi C. Saido, Murali Jayapala, Patrick De Boever (+5 others)
2021 Acta Neuropathologica Communications  
Furthermore, we show that hyperspectral imaging can be used to quantify retinal Aβ, underscoring its potential as a biomarker for AD diagnosis and monitoring.  ...  AbstractIn this study, we report the results of a comprehensive phenotyping of the retina of the AppNL-G-F mouse.  ...  advice on hyperspectral imaging;  ... 
doi:10.1186/s40478-020-01102-5 pmid:33407903 pmcid:PMC7788955 fatcat:lbycxlwmcrabzngdbqvjgr33ky

Biomedical Applications of Translational Optical Imaging: From Molecules to Humans

Daniel L. Farkas
2021 Molecules  
detail the makeup and functioning of the living state can and should be combined for maximum effect, especially if one seeks simultaneously high spatiotemporal resolution and discrimination ability within  ...  Its multitude of features (intensity, wavelength, polarization, interference, coherence, timing, non-linear absorption, and even interactions with itself) able to create contrast, and thus images that  ...  The specimens were then imaged at high resolution using laser-scanning confocal microscopy.  ... 
doi:10.3390/molecules26216651 pmid:34771060 pmcid:PMC8587670 fatcat:epwtmw4l5felvltnlaas4gwwqq

Pericyte degeneration leads to neurovascular uncoupling and limits oxygen supply to brain

Kassandra Kisler, Amy R Nelson, Sanket V Rege, Anita Ramanathan, Yaoming Wang, Ashim Ahuja, Divna Lazic, Philbert S Tsai, Zhen Zhao, Yi Zhou, David A Boas, Sava Sakadžić (+1 others)
2017 Nature Neuroscience  
Thus, pericyte degeneration as seen in neurological disorders such as Alzheimer's disease may contribute to neurovascular dysfunction and neurodegeneration associated with human disease.  ...  Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:  ...  This research was supported by National Institutes of Health grants R01 AG023084, R01 AG039452, R01 NS034467 and R01 NS100459 to B.V.Z.; R01 NS091230 to  ... 
doi:10.1038/nn.4489 pmid:28135240 pmcid:PMC5323291 fatcat:gw6wa42gwnchta3jer5nsgu4pe

Neuronal metabolic rewiring promotes resilience to neurodegeneration caused by mitochondrial dysfunction

E. Motori, I. Atanassov, S. M. V. Kochan, K. Folz-Donahue, V. Sakthivelu, P. Giavalisco, N. Toni, J. Puyal, N.-G. Larsson
2020 Science Advances  
Neurodegeneration in mitochondrial disorders is considered irreversible because of limited metabolic plasticity in neurons, yet the cell-autonomous implications of mitochondrial dysfunction for neuronal  ...  Our findings identify a previously unappreciated pathway conferring resilience to mitochondrial dysfunction and show that neurodegeneration can be reversed even at advanced disease stages.  ...  Transgenic mouse models bearing mutated genes, which are associated with human spinocerebellar ataxia, have also been reported to have mitochondrial dysfunction (49, 50) , emphasizing the importance of  ... 
doi:10.1126/sciadv.aba8271 pmid:32923630 pmcid:PMC7455195 fatcat:cpt2acjntvcqlfnogo3xlnzejm

Proceedings of the Joint Meeting of the 126th Annual Meeting of The Japanese Association of Anatomists and the 98th Annual Meeting of The Physiological Society of Japan

2021 Journal of Physiological Sciences  
neuronal synapses and mitochondria modulated by postnatal environment Yoshiaki Shinohara 1 , Nobuhiko Ohno 1 ( ) (COI:No) SY18-1 Large-scale electron microscopy and imaging big data Yosky Kataoka  ...  technologies Tetsu Tanaka 1 ( ) (COI:No) Symposium6 SY7-4 Large-scale electron microscopic volume imaging of interfascicular oligodendrocytes in the mouse corpus callosum Tatsuhide Tanaka 1 , Nobuhiko  ... 
doi:10.1186/s12576-021-00809-x fatcat:nh2jab2kgne67bsk2vrksdflya

1st Annual Meeting: The Canadian Association for Neuroscience / Association canadienne de neuroscience

2007 Canadian Journal of Neurological Sciences  
using multiphoton fluorescence microscopy.  ...  Unilateral transient focal cerebral ischemia was induced by intraluminal filament occlusion of the left MCA during 1 hour followed by imaging session with a high resolution/high sensitivity CCD camera  ... 
doi:10.1017/s0317167100017066 fatcat:aupmr4ntyjd6xes4gp5epa2epm

Abstract Book, XIII International Congress of Histochemistry and Cytochemistry ICHC2008, Imaging of Cell Dynamics, 23rd-27th August 2008, Medical University of Gdansk, Poland

FHC Redaction
2008 Folia Histochemica et Cytobiologica  
disorders using animal models and/or human post-mortem tissue.  ...  Here, we report that KS is expressed by microglia in spinal cord of SOD-G93A transgenic mouse (ALS model mouse).  ...  To reach this goal, changes in the chemical coding of sympathetic neurons supplying porcine urinary bladder, an animal which can be used as a very good model for human lower urinary tract, have been elucidated  ... 
doi:10.5603/4388 doaj:cdebe86bf2604301b2401e99ae9a504c fatcat:7j6nazvgnjf2bhooy5awdzq7uy
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