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Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD

Kevin O'Brien, Ming Zhao, David Nolte
2016 Micromachines  
Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk.  ...  Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parameters.  ...  Abbreviations The following abbreviations are used in this manuscript: BioCD Biological compact disc APDMES Aminopropaldimethyl ethoxy silane PBS Phosphate buffered saline SDI Spinning disk interferometry  ... 
doi:10.3390/mi7020031 pmid:30407404 pmcid:PMC6190026 fatcat:iledxvmjerf5vkaf2mfjslu37q

Invited Review Article: Review of centrifugal microfluidic and bio-optical disks

David D. Nolte
2009 Review of Scientific Instruments  
The two main classes of spinning biodisks are microfluidic disks and bio-optical compact disks ͑BioCD͒.  ...  BioCDs use spinning-disk interferometry, under the condition of common-path phase quadrature, to perform interferometric label-free detection of molecular recognition and binding.  ...  ACKNOWLEDGMENTS This work was supported in part by Quadraspec, Inc., the Indiana Economic Development Corporation, and NIH NCI Grant No. R21CA125336.  ... 
doi:10.1063/1.3236681 pmid:19895047 pmcid:PMC2782362 fatcat:qj5ijrybjrd4rb52bqhzk3xwly

High-Speed Interferometric Detection of Label-Free Immunoassays on the Biological Compact Disc

M. Zhao, D. Nolte, W. Cho, F. Regnier, M. Varma, G. Lawrence, J. Pasqua
2006 Clinical Chemistry  
Protein on the disc is scanned by a focused laser spot as the disc spins.  ...  The binding of antigen or antibodies is detected directly, without labels, by a change in the interferometric intensity.  ...  This work was performed through a sponsored research grant through the Purdue Research Foundation from QuadraSpec, Inc.  ... 
doi:10.1373/clinchem.2006.072793 pmid:16990417 fatcat:mz2gpqf5fvetfixw2vbf3dtyoy

Differential phase-contrast BioCD biosensor

Ming Zhao, Wonryeon Cho, Fred Regnier, David Nolte
2007 Applied Optics  
Common-path differential phase-contrast interferometry measures the spatial gradient of surface dipole density on a bio-optical compact disk (BioCD) and is sensitive to small changes in dipole density  ...  The recognition molecules are antibody IgG proteins that are deposited in periodic patterns on the BioCD using soft lithography or photolithography on the silanized silica surfaces of dielectric mirrors  ...  This work was supported by sponsored research from QuadraSpec, Inc., through the Purdue Research Foundation.  ... 
doi:10.1364/ao.46.006196 pmid:17712386 fatcat:z6qsf6ntw5fwdlsguvx6sf4nu4

Adaptive interferometry of protein on a BioCD

Leilei Peng, Manoj M. Varma, Wonryeon Cho, Fred E. Regnier, David D. Nolte
2007 Applied Optics  
Adaptive spinning-disk interferometry is capable of measuring surface profiles of a thin biolayer with subnanometer longitudinal resolution.  ...  We performed biosensing of immobilized monolayers of antibodies on the disk in both transmission and reflection detection modes.  ...  This work was supported by NSF grant ECS-0200424, and by research sponsored by QuadraSpec, Inc. through the Purdue Research Foundation.  ... 
doi:10.1364/ao.46.005384 pmid:17676154 fatcat:u24dliiumrd7tpkuvwgindzdju

Common-path interferometric detection of protein monolayer on the BioCD

Xuefeng Wang, Ming Zhao, D. D. Nolte
2007 Applied Optics  
The bio-optical compact disk (BioCD) is an optical biosensor that performs common-path molecular interferometry of patterned proteins on a disk spinning at high speed.  ...  The scaling mass sensitivity of the IL channel on oxide on silicon was measured to be 0.17 pg͞mm.  ...  The radius of the focal spot is ϳ18 m on the disk. Higher resolution can be achieved by switching the 10 cm lens with a short focal-length lens.  ... 
doi:10.1364/ao.46.007836 pmid:17994133 fatcat:s6spbgzdorb7nbxrsllkchrt7i

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Günter Gauglitz
2020 Analytical and Bioanalytical Chemistry  
Improvement of optics is only one way to increase the quality of biosensors.  ...  by different companies.  ...  Spinning disc The idea of backscattering by nanostructured surfaces was used for a new class of analytical sensors to detect immobilized biomolecules with high speed and high sensitivity by using a spinning-disc  ... 
doi:10.1007/s00216-020-02581-0 pmid:32313998 fatcat:qukey6tyhbbj3b4oykkbyzwzu4