A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
With the rapid progress of China's urbanization, research on the automatic detection of land-use patterns in Chinese cities is of substantial importance. Deep learning is an effective method to extract image features. To take advantage of the deep-learning method in detecting urban land-use patterns, we applied a transfer-learning-based remote-sensing image approach to extract and classify features. Using the Google Tensorflow framework, a powerful convolution neural network (CNN) library wasarXiv:1708.01580v1 fatcat:roc2h6ape5c3dbcjv35qmlrtwm
more »... eated. First, the transferred model was previously trained on ImageNet, one of the largest object-image data sets, to fully develop the model's ability to generate feature vectors of standard remote-sensing land-cover data sets (UC Merced and WHU-SIRI). Then, a random-forest-based classifier was constructed and trained on these generated vectors to classify the actual urban land-use pattern on the scale of traffic analysis zones (TAZs). To avoid the multi-scale effect of remote-sensing imagery, a large random patch (LRP) method was used. The proposed method could efficiently obtain acceptable accuracy (OA = 0.794, Kappa = 0.737) for the study area. In addition, the results show that the proposed method can effectively overcome the multi-scale effect that occurs in urban land-use classification at the irregular land-parcel level. The proposed method can help planners monitor dynamic urban land use and evaluate the impact of urban-planning schemes.
Aiming at the problems of feature point calibration method of 3D light detection and ranging (LiDAR) and camera calibration that are calibration boards in various forms, incomplete information extraction methods and large calibration errors, a novel calibration board with local gradient depth information and main plane square corner information (BWDC) was designed. In addition, the "three-step fitting interpolation method" was proposed to select feature points and obtain the correspondingdoi:10.3390/s20041130 pmid:32092995 pmcid:PMC7070607 fatcat:sav6zkxbpzg2havcy6hesnxqwm
more »... nates of feature points in the LiDAR coordinate system and camera pixel coordinate system based on BWDC. Finally, calibration experiments were carried out, and the calibration results were verified by methods such as incremental verification and reprojection error comparison. The calibration results show that using BWDC and the "three-step fitting interpolation method" can solve quite accurate coordinate transformation matrix and intrinsic and external parameters of sensors, which dynamically change within 0.2% in the repeatable experiments. The difference between the experimental value and the actual value in the incremental verification experiment is about 0.5%. The average reprojection error is 1.8312 pixels, and the value changes at different distances do not exceed 0.1 pixels, which also show that the calibration method is accurate and stable.
Rolling element bearings are an important unit in the rotating machines, and their performance degradation assessment is the basis of condition-based maintenance. Targeting the non-linear dynamic characteristics of faulty signals of rolling element bearings, a bearing performance degradation assessment approach based on improved fuzzy entropy (FuzzyEn) is proposed in this paper. FuzzyEn has less dependence on data length and achieves more freedom of parameter selection and more robustness todoi:10.1515/msr-2017-0026 fatcat:yglmunzqhjfmzjj6bes3eyzy3q
more »... se. However, it neglects the global trend of the signal when calculating similarity degree of two vectors, and thus cannot reflect the running state of the rolling element bearings accurately. Based on this consideration, the algorithm of FuzzyEn is improved in this paper and the improved FuzzyEn is utilized as an indicator for bearing performance degradation evaluation. The vibration data from run-to-failure test of rolling element bearings are used to validate the proposed method. The experimental results demonstrate that, compared with the traditional kurtosis and root mean square, the proposed method can detect the incipient fault in advance and can reflect the whole performance degradation process more clearly.
., Liang, Z., Hou, G., Song, L., Zhang, R., Gan, Y. et al. (2017) N-cadherin-mediated activation of PI3K/Akt-GSK-3beta signaling attenuates nucleus pulposus cell apoptosis under high-magnitude compression ...doi:10.1042/bsr20190163 pmid:30910846 pmcid:PMC6470409 fatcat:yebibhyjqjfcle7il4bcbun32y
Sinusoidal phase-modulated signal light through the interferometer can produce a beat signal. Moreover, its amplitude monotonically changes with the signal light frequency. So the beat signal amplitude can be used to measure laser-Doppler frequency shift. In addition to the beat signal, the phase-modulated signal also contains a direct current signal, and it still contains a large amount of frequency shift information, but the information is not utilized, resulting in the waste of information.doi:10.1088/1742-6596/1149/1/012017 fatcat:rqwdp33ilbgebf6ytn2z3zt2ta
more »... n this paper, this kind of phase-modulated laser-Doppler frequency shift measurement method is improved to simultaneously utilize the useful information in the DC and beat signal for the frequency shift measurement. The specific method is to use the ratio of beat signal amplitude to DC signal amplitude to define a new parameter used in frequency shift measurement. The signal light intensity terms in DC and beat signal can be eliminated, so the improved measurement method does not need to measurement the signal light intensity, which makes its structure further simplified. By comparing the frequency change curves between the newly defined parameter and the beat signal amplitude theoretically, we find that they have the same distribution rule. In theory, by comparing the measurement sensitivity curves, it is proved that the improved phase-modulated frequency shift measurement method has higher measurement sensitivity and dynamic range than the unimproved one.
A highly ordered mesoporous carbon (HOMC) has been synthesized through a strongly acidic, aqueous cooperative assembly route. The structure and morphology of the carbon material were investigated using TEM, SEM, and nitrogen adsorption-desorption isotherms. The carbon was proven to be meso-structural and consisted of graphitic microdomain with larger interlayer space. Active carbon impedance and electrochemical measurements reveal that the synthesized highly ordered mesoporous carbon (HOMC)doi:10.3389/fenrg.2014.00042 fatcat:qsugtiaxejfwrodfu7yewlfoce
more »... bits a promoted electrochemical hydrogen insertion process and improved capacitance and hydrogen storage stability. The meso-structure and enlarged interlayer distance within the HOMC are suggested as possible causes for the enhancement in hydrogen storage. Both hydrogen capacity in the carbon and mass diffusion within the matrix were improved.
Objective: To evaluate damage to the extracorticospinal tract in Wilson disease (WD) patients using diffusion tensor imaging (DTI). Methods: 70 patients with WD, including 50 with cerebral type and 20 with hepatic type, and 20 age-matched healthy controls were enrolled. Neurological symptoms were scored using the modified Young Scale. Patients with cerebral type WD were divided into four subgroups: those with (1) hypokinesia, (2) parkinsonism, (3) mouth and throat dystonia, and (4) psychiatricdoi:10.1016/j.jns.2016.02.006 pmid:26944166 fatcat:t3hf4ubjd5dunfqzmiwambkwpa
more »... ymptoms. All study subjects underwent DTI of the brain. Five regions of interest (ROIs) were chosen. Fractional anisotropy (FA) and fiber volumes between ROIs were determined, and the relationships between DTI metrics and clinical status were evaluated. Results: FA values and fiber volumes between subcortical nuclei were lower in WD patients. Fiber volumes between the putamen (PU) and the globus pallidus (GP), substantia nigra (SN), and thalamus (TH); between the head of the caudate nucleus (CA) and the GP and TH; and between the TH and cerebellum were lower in group 1 than in the other groups of WD patients. Fiber volumes between the GP and the SN and TH were lower in group 2, and fiber volumes between the SN and TH were lower in group 3. DTI metrics differed between patients with the cerebral and hepatic types of WD. Conclusions: DTI can reconstruct the network of the extracorticospinal tract. Fiber projection between subcortical nuclei was abnormal in WD patients. Damage to fiber connections may correlate with neurological symptoms in WD patients.
Mechanical overload is a risk factor of disc degeneration. It can induce disc degeneration through mediating cell apoptosis. Mechano growth factor (MGF) has been reported to inhibit mechanical overload-induced apoptosis of chondrocytes. The present study is aimed to investigate whether MGF can attenuate mechanical overload-induced nucleus pulposus (NP) cell apoptosis and the possible signaling transduction pathway. Rat NP cells were cultured and subjected to mechanical overload for 7 days. Thedoi:10.1042/bsr20182462 pmid:30858307 pmcid:PMC6438874 fatcat:koygtszd2nghrfpvojys5xiepa
more »... ontrol NP cells did not experience mechanical load. The exogenous MGF peptide was added into the culture medium to investigate its protective effects. NP cell apoptosis ratio, caspase-3 activity, gene expression of Bcl-2, Bax and caspase-3, protein expression of cleaved caspase-3, cleaved PARP, Bax and Bcl-2 were analyzed to evaluate NP cell apoptosis. In addition, activity of the p38 MAPK pathway was also detected. Compared with the control NP cells, mechanical overload significantly increased NP cell apoptosis and caspase-3 activity, up-regulated gene/protein expression of pro-apoptosis molecules (i.e. Bax, caspase-3, cleaved caspase-3 and cleaved PARP) whereas down-regulated gene/protein expression of anti-apoptosis molecule (i.e. Bcl-2). However, exogenous MGF partly reversed these effects of mechanical overload on NP cell apoptosis. Further results showed that activity of the p38 MAPK pathway of NP cells cultured under mechanical overload was decreased by addition of MGF peptide. In conclusion, MGF is able to attenuate mechanical overload-induced NP cell apoptosis, and the p38 MAPK signaling pathway may be involved in this process. The present study provides that MGF supplementation may be a promising strategy to retard mechanical overload-induced disc degeneration.
The free radical, or oxidative stress, theory posits that imbalance in cells between prooxidants and antioxidants results in an altered redox state and, over time, an accumulation of oxidative damage. We hypothesized herein that cells with an increasingly prooxidant intracellular environment also might be particularly susceptible to acute oxidative stress. To test this hypothesis, MA-10 cells were used as a model because of their well-defined, measurable function, namely progesteronedoi:10.1016/j.mce.2010.02.034 pmid:20206230 pmcid:PMC2875365 fatcat:veop6w2hdbfabjnw5tm6t55qxq
more »... We first experimentally altered the redox environment of the cells by their incubation with buthionine sulfoximine (BSO) or diethyl maleate (DEM) so as to deplete glutathione (GSH), and then exposed the GSH-depleted cells acutely to the prooxidant tert-butyl hydroperoxide (t-BuOOH). Neither BSO or DEM by themselves affected progesterone production. However, when the GSH-depleted cells subsequently were exposed acutely to t-BuOOH, intracellular reactive oxygen species concentration was significantly increased, and this was accompanied by significant reductions in progesterone production. In striking contrast, treatment of control cells with t-BuOOH had no effect. Depletion of GSH and subsequent treatment of the cells with t-BuOOH-induced the phosphorylation of each of ERK1/2, JNK and p38, members of the MAPK family. Inhibition of p38 phosphorylation largely prevented the t-BuOOH-induced down-regulation of progesterone production in GSH-depleted cells. These results suggest that, as hypothesized, alteration of the intracellular GSH redox environment results in the increased sensitivity of MA-10 cells to oxidative stress, and that this is mediated by activation of one or more redox-sensitive MAPK members.
Previous studies have shown that phthalate exposure can suppress steroidogenesis. However, the affected components of the steroidogenic pathway, and the mechanisms involved, remain uncertain. We show that incubating MA-10 Leydig cells with mono-(2-ethylhexyl) phthalate (MEHP) resulted in reductions in luteinizing hormone (LH)-stimulated cAMP and progesterone productions. cAMP did not decrease in response to MEHP when the cells were incubated with cholera toxin or forskolin. Incubation ofdoi:10.1016/j.reprotox.2013.07.025 pmid:23969005 pmcid:PMC3836854 fatcat:2ykdkl6tgbgojg5v3o7ccmyeem
more »... eated cells with dibutyryl-cAMP, 22hydroxycholesterol or pregnenolone inhibited the reductions in progesterone. Increased levels of reactive oxygen species (ROS) occurred in response to MEHP. In cells in which intracellular glutathione was depleted by buthionine sulfoximine pretreatment, the increases in ROS and decreases in progesterone in response to MEHP treatment were exacerbated. These results indicate that MEHP inhibits MA-10 Leydig cell steroidogenesis by targeting LH-stimulated cAMP production and cholesterol transport, and that a likely mechanism by which MEHP acts is through increased oxidative stress.
Emerging Liquid Crystal Technologies XIII
We proposed a self-calibration method to calibrate both the phase-voltage look-up table and the screen phase distribution of Liquid Crystal on Silicon (LCoS) displays by implementing different lens configurations on the studied device within a same optical scheme. On the one hand, the phase-voltage relation is determined from interferometric measurements, which are obtained by addressing split-lens phase distributions on the LCoS display. On the other hand, the surface profile is retrieved bydoi:10.1117/12.2290434 fatcat:754b3wd43jbdrpbzjxzsj3nxie
more »... lf-addressing a diffractive micro-lens array to the LCoS display, in a way that we configure a Shack-Hartmann wavefront sensor that self-determines the screen spatial variations. Moreover, both the phase-voltage response and the surface phase inhomogeneity of the LCoS are measured within the same experimental set-up, without the necessity of further adjustments. Experimental results prove the usefulness of the above-mentioned technique for LCoS displays characterization.
Spermatozoa are produced within the seminiferous tubules after sexual maturity. The expression levels of mRNAs and lncRNAs in testicular tissues are different at each stage of testicular development and are closely related to formation of the extracellular matrix and spermatogenesis. Therefore, we set out to study the expression of lncRNAs and mRNAs during the different developmental stages of the goat testis. We constructed 12 RNA libraries using testicular tissues from goats aged 3, 6, and 12doi:10.5713/ab.21.0259 pmid:34530511 pmcid:PMC8902208 fatcat:3v3nooklpfekdoepo6za735jhe
more »... months, and studied the functions of mRNAs and lncRNAs using the Gene Ontogeny (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Relationships between differentially expressed genes (DEGs) were analyzed by lncRNA-mRNA co-expression network and protein-protein interaction network (PPI). Finally, the protein expression levels of matrix metalloproteinase 2 (MMP2), insulin-like growth factor 2 (IGF2), and insulin-like growth factor-binding protein 6 (IGFBP6) were detected by western blotting. We found 23, 8, and 135 differentially expressed lncRNAs and 161, 12, and 665 differentially expressed mRNAs that were identified between 3 vs. 6, 6 vs. 12, and 3 vs. 12 months, respectively. GO, KEGG, and PPI analyses showed that the differential genes were mainly related to the extracellular matrix. Moreover, MMP2 was a hub gene and co-expressed with the lncRNA TCONS-0002139 and TCONS-00093342. The results of qRT-PCR verification were consistent with those of RNA-seq sequencing. The expression trends of MMP2, IGF2, and IGFBP6 protein were the same as that of mRNA, which all decreased with age. IGF2 and MMP2 were significantly different in the 3 vs. 6-month-old group (P < 0.05). These results improve our understanding of the molecular mechanisms involved in sexual maturation of the goat testis.
Journal of Cancer
Author contributions Leifeng Liang and Qingwen Huang organized and wrote the manuscript. Leifeng Liang designed and produced the figures. ... Haolin Yan, Zhan Lin, Haisheng Zhu, Rensheng Wang, and Kai Hu revised the manuscript. All authors reviewed the manuscript and approved the manuscript for publication. ...doi:10.7150/jca.51467 pmid:34093796 pmcid:PMC8176234 fatcat:2jyu53eaprh3pkhj67ncwqhyvm
Using citric acid (CA) and ethylenediamine (EDA) as precursors, stable nitrogen-doped carbon dots (CD) nanosols were prepared by microwave procedure and characterized in detail. It was found that CDNs catalyze ethanol (Et)-HAuCl4 to generate gold nanoparticles (AuNPs), which have strong surface plasmon resonance, Rayleigh scattering, (RRS) and a surface plasmon resonance (SPR) absorption (Abs) effect at 370 nm and 575 nm, respectively. Compled the new catalytic amplification indicator reactiondoi:10.3390/molecules26195930 pmid:34641474 fatcat:e6gzwi63pbgorihnjmhndd5vre
more »... ith the specific As3+ aptamer reaction, a new RRS/Abs dual-mode aptamer sensor for the assay of trace As3+ was developed, based on the RRS/Abs signals increasing linearly with As3+ increasing in the ranges of 5–250 nmol/L and 50−250 nmol/L, whose detection limits were 0.8 nmol/L and 3.4 nmol/L As3+, respectively. This analytical method has the advantages of high selectivity, simplicity, and rapidity, and it has been successfully applied to the detection of practical samples.
Zhenlin Liang analyzed the data, authored or reviewed drafts of the paper, and approved the final draft. ... Haolin Yu performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft. ...doi:10.7717/peerj.12705 pmid:35036171 pmcid:PMC8740510 fatcat:kimyezkeu5aodbs4cemniiz2qi
« Previous Showing results 1 — 15 out of 52 results