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Toxicarioside H induces drug-resistant mitophagy via promoting expression of sirtuin-3 in lung cancer cells

Feng-Ying Huang, Yan Sun, Yong-Le Yang, Yue-Nan Li, Wen-Li Mei, Jing Lei, Cai-Chun Wang, Quan Liu, Ying-Ying Lin, Yong-Hao Huang, Hao-Fu Dai, Guang-Hong Tan
2018 OncoTarget  
Copyright: Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Cardenolides may have anticancer effects and toxicarioside H (ToxH), which we isolated, may have similar activity but its underlying mechanism against tumors is not clear. Herein, we report that ToxH treatment
more » ... ited cell proliferation and induced mitochondrion-dependent apoptosis and mitophagy in human A549 and H460 lung cancer cells. ToxH treatment increased the expression of Sirt3, and inhibited Sirt3 expression via RNA interference against Sirt3 (siSirt3) suppressed mitophagy in ToxH-treated lung cancer cells. Stronger inhibition of cell proliferation and induction of apoptosis occurred when ToxH-mediated mitophagy was blocked by siSirt3. In addition, ToxH treatment redistributed hexokinase II from the mitochondria to the cytosol in A549 and H460 lung cancer cells, and decreased interaction of hexokinase II with VDAC1 was accompanied by the increased interaction of VDAC1 with parkin. Moreover, the disruption of Sirt3 by siSirt3 decreased the association of VDAC1 with parkin. Thus, ToxH induces drug-resistant mitophagy by enhancing the expression of Sirt3, which leads to the dissociation of hexokinase II from VDAC1 and increased binding of VDAC1 with parkin. Adding ToxH to a mitophagy inhibitor may thus be an effective strategy for treating lung cancer.
doi:10.18632/oncotarget.24154 fatcat:3dk2qw2xxnhsxgenewuq5zoxom

Cd-induced changes in leaf proteome of the hyperaccumulator plant Phytolacca americana

Le Zhao, Yong-Le Sun, Su-Xia Cui, Mei Chen, Hao-Meng Yang, Hui-Min Liu, Tuan-Yao Chai, Fang Huang
2011 Chemosphere  
Cadmium (Cd) is highly toxic to all organisms. Soil contamination by Cd has become an increasing problem worldwide due to the intensive use of Cd-containing phosphate fertilizers and industrial zinc mining. Phytolacca americana L. is a Cd hyperaccumulator plant that can grow in Cd-polluted areas. However, the molecular basis for its remarkable Cd resistance is not known. In this study, the effects of Cd exposure on protein expression patterns in P. americana was investigated by 2-dimensional
more » ... electrophoresis (2-DE). 2-DE profiles of leaf proteins from both control and Cd-treated (400 lM, 48 h) seedlings were compared quantitatively using ImageMaster software. In total, 32 differentially expressed protein spots were identified using MALDI-TOF/TOF mass spectrometry coupled to protein database search, corresponding to 25 unique gene products. Of those 14 were enhanced/induced while 11 reduced under Cd treatment. The alteration pattern of protein expression was verified for several key proteins involved in distinct metabolic pathways by immuno-blot analysis. Major changes were found for the proteins involved in photosynthetic pathways as well as in the sulfur-and GSH-related metabolisms. One-third of the up-regulated proteins were attributed to transcription, translation and molecular chaperones including a protein belonging to the calreticulin family. Other proteins include antioxidative enzymes such as 2-cys-peroxidase and oxidoreductases. The results of this proteomic analysis provide the first and primary information regarding the molecular basis of Cd hypertolerance in P. americana.
doi:10.1016/j.chemosphere.2011.06.029 pmid:21723586 fatcat:7rjrtyhodzeq5dykp524cmheea

Implementation of non-local operations for arbitrary high-dimensional systems with qubit quantum channel [article]

Hao-Sheng Zeng, Yong-Guang Shan, Jian-Jun Nie, Le-Man Kuang
2005 arXiv   pre-print
We propose a method to implement a kind of non-local operations between spatially separated two systems with arbitrary high-dimensions by using only low-dimensional qubit quantum channels and classical bit communications. The result may be generalized straightforwardly to apply for multiple systems, each of them with arbitrary dimensions. Compared with existed approaches, our method can economize classical resources and the needed low-dimensional quantum channels may be more easily established
more » ... n practice. We also show the construction of the non-local quantum XOR gate for qutrit systems in terms of the obtained non-local operations as well as some single qutrit local gates.
arXiv:quant-ph/0508054v1 fatcat:e7uwfjhsxbgdlnxc4kwl3nlzl4

Kainate receptor activation induces glycine receptor endocytosis through PKC deSUMOylation

Hao Sun, Li Lu, Yong Zuo, Yan Wang, Yingfu Jiao, Wei-Zheng Zeng, Chao Huang, Michael X. Zhu, Gerald W. Zamponi, Tong Zhou, Tian-Le Xu, Jinke Cheng (+1 others)
2014 Nature Communications  
Surface expression and regulated endocytosis of glycine receptors (GlyRs) play a critical function in balancing neuronal excitability. SUMOylation (SUMO modification) is of critical importance for maintaining neuronal function in the central nervous system. Here we show that activation of kainate receptors (KARs) causes GlyR endocytosis in a calcium-and protein kinase C (PKC)-dependent manner, leading to reduced GlyR-mediated synaptic activity in cultured spinal cord neurons and the superficial
more » ... dorsal horn of rat spinal cord slices. This effect requires SUMO1/sentrin-specific peptidase 1 (SENP1)-mediated deSUMOylation of PKC, indicating that the crosstalk between KARs and GlyRs relies on the SUMOylation status of PKC. SENP1-mediated deSUMOylation of PKC is involved in the kainate-induced GlyR endocytosis and thus plays an important role in the anti-homeostatic regulation between excitatory and inhibitory ligand-gated ion channels. Altogether, we have identified a SUMOylation-dependent regulatory pathway for GlyR endocytosis, which may have important physiological implications for proper neuronal excitability.
doi:10.1038/ncomms5980 pmid:25236484 pmcid:PMC4199113 fatcat:op6j4e42tffpvc3iskaofayb3q

A new indole alkaloid with anti-inflammatory from the branches of Nauclea officinalis

Le-Ling Song, Yan-Ling Mu, Hao-Chao Zhang, Gui-Ying Wu, Jing-Yong Sun
2018 Figshare  
A new indole alkaloid, 17-oxo-19-(Z)-naucline, and six known alkaloids 2–7 were isolated from the branches of Nauclea officinalis. The structure of the new compound 1 was characterised mainly by analysing its physical data including IR, 1 D, 2 D NMR, and HR-ESI-MS. Other compounds were identified by comparisons their data with those reported in the literature. Compound1, 4, 5, 6, 7 showed in vitro anti-inflammatory activity decrease the LPS-stimulated production of nitric oxide in RAW264.7
more » ... while all compounds exhibited weak cytotoxicity against human tumour cell lines (LOVO, A549 and HepG2).
doi:10.6084/m9.figshare.7503572 fatcat:bngpc2uzgfdljfuyxy7bmb2po4

MGMDcGAN: Medical Image Fusion Using Multi-generator Multi-discriminator Conditional Generative Adversarial Network

Jun Huang, Zhuliang Le, Yong Ma, Fan Fan, Hao Zhang, Lei Yang
2020 IEEE Access  
ZHULIANG LE received the B.E. degree from the School of Information Engineering, Wuhan University of Technology, Wuhan, China, in 2019.  ...  HAO ZHANG received the B.E. degree from the School of Mechanical Engineering and Electronic Information, China University of Geosciences, Wuhan, China, in 2019.  ... 
doi:10.1109/access.2020.2982016 fatcat:l7syq23cjjbq7esnpacjjosluu

Ethanol Extract of Centipeda minima Exerts Antioxidant and Neuroprotective Effects via Activation of the Nrf2 Signaling Pathway

Yi-Jie Wang, Xin-Yue Wang, Xu-Yi Hao, Yong-Ming Yan, Ming Hong, Su-Fen Wei, Yi-Le Zhou, Qi Wang, Yong-Xian Cheng, Yong-Qiang Liu
2019 Oxidative Medicine and Cellular Longevity  
Oxidative stress is implicated in the pathogenesis of neurodegeneration and other aging-related diseases. Previous studies have found that the whole herb of Centipeda minima has remarkable antioxidant activities. However, there have been no reports on the neuroprotective effects of C. minima, and the underlying mechanism of its antioxidant properties is unclear. Here, we examined the underlying mechanism of the antioxidant activities of the ethanol extract of C. minima (ECM) both in vivo and in
more » ... vitro and found that ECM treatment attenuated glutamate and tert-butyl hydroperoxide (tBHP)-induced neuronal death, reactive oxygen species (ROS) production, and mitochondria dysfunction. tBHP-induced phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinases (JNK) was reduced by ECM, and ECM sustained phosphorylation level of extracellular signal regulated kinase (ERK) in SH-SY5Y and PC12 cells. Moreover, ECM induced the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the upregulation of phase II detoxification enzymes, including heme oxygenase-1 (HO-1), superoxide dismutase-2 (SOD2), and NAD(P)H quinone oxidoreductase-1 (NQO-1) in both two cell types. In a D-galactose (D-gal) and aluminum muriate (AlCl3)-induced neurodegenerative mouse model, administration of ECM improved the learning and memory of mice in the Morris water maze test and ameliorated the effects of neurodegenerative disorders. ECM sustained the expression level of postsynaptic density 95 (PSD95) and synaptophysin (SYN), activated the Nrf2 signaling pathway, and restored the levels of cellular antioxidants in the hippocampus of mice. In addition, four sesquiterpenoids were isolated from C. minima to identify the bioactive components responsible for the antioxidant activity of C. minima; 6-O-angeloylplenolin and arnicolide D were found to be the active compounds responsible for the activation of the Nrf2 signaling pathway and inhibition of ROS production. Our study examined the mechanism of C. minima and its active components in the amelioration of oxidative stress, which holds the promise for the treatment of neurodegenerative disease.
doi:10.1155/2019/9421037 fatcat:dqyhqekvtjf2zeftwci36hortu


Ding Ying-Ru, Yen Qi-Jie, Hsia Yuan-Fu, Yeh Shun-Hao, Chen Yi, Jin Yong-Shu, Qiu Jin-Heng
1980 Le Journal de Physique Colloques  
doi:10.1051/jphyscol:19801127 fatcat:nhzaruabibet5jubgintosvjsu

Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

Hao Zhang, Guo Hu Cheng, Yong Jun Li, Miao Yin Cai, Hai Yan Guo, Kang Le Qin
2017 World Rabbit Science  
<p>To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR) was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM) together with fertilisation (IVF) was conducted immediately after superovulation. The results showed that (1) the age factor significantly
more » ... ected superovulation in young rabbit females, with 60 d as an optimal age; (2) the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3) the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4) the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.</p>
doi:10.4995/wrs.2017.4485 fatcat:x32ck5gpz5ajtjs3h6bu45uf4a

Can azulene-like molecules function as substitution-free molecular rectifiers?

Kai-Ge Zhou, Yong-Hui Zhang, Le-Jia Wang, Ke-Feng Xie, Yu-Qing Xiong, Hao-Li Zhang, Cheng-Wei Wang
2011 Physical Chemistry, Chemical Physics - PCCP  
doi:10.1039/c0cp02693j pmid:21822508 fatcat:l7pbvbcq4ffkxgxrtfzefx3ege

Preliminary clinical results for PET/MR compared with PET/CT in patients with nasopharyngeal carcinoma

Yong Cheng, Le Bai, Jingjie Shang, Yongjin Tang, Xueying Ling, Bin Guo, Jian Gong, Lu Wang, Hao Xu
2019 Oncology Reports  
The bi-exponential model from an IVIM sequence was expressed using the following equation, as described by Le et al (45) : Sb/S0=(1-f) exp (-b D) + f exp [-b (D * + D)].  ...  the motion of water molecules due to perfusion in the microcirculation from that due to diffusion in the extravascular space, the intravoxel incoherent motion (IVIM) imaging method, first described by Le  ... 
doi:10.3892/or.2019.7392 pmid:31746412 pmcid:PMC6908933 fatcat:oflvrts6wnghfmu5sbco2zlote

Risk factors for osteoporosis in liver cirrhosis patients measured by transient elastography

Jian-ping Zheng, Hai-xiong Miao, Shao-wei Zheng, Wei-le Liu, Chu-qun Chen, Hao-bo Zhong, Sheng-fa Li, Yong-ping Fang, Chun-han Sun
2018 Medicine  
Osteoporosis or osteopenia is a common complication in patients with cirrhosis, but little is known about the risk factors for the occurrence of osteoporosis. Patients with liver cirrhosis due to chronic virus infection and alcoholic abuse were enrolled. Bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry (DXA). Osteoporosis was diagnosed according to WHO criteria. The severity of liver stiffness was measured by Fibroscan. Demographic data, such as age, gender,
more » ... t, height, and body mass index (BMI), were collected. Logistic regression analysis was used to recognize the risk factors of osteoporosis in patients with cirrhosis. A total of 446 patients were included in this study: 217 had liver cirrhosis (male, 74.2%; mean age, 57.2 ± 10.27) and 229 were matched controls (male, 69%, mean age, 56.69 ± 9.37). Osteoporosis was found in 44 patients (44/217, 20.3%). The spine and hip BMD in cirrhotic patients were significantly lower than that in controls. When the cirrhotic and control subjects were stratified by age, gender, and BMI, the significant difference was also observed in women patients, patients older than 60, and patients with BMI < 18. Multivariate analysis showed that the older age [odds ratio (OR) = 1.78, P = .046], lower BMI (OR = 0.63, P = .049), greater fibroscan score (OR = 1.15, P = .009), and liver cirrhosis induced by alcohol liver disease (OR = 3.42, P < .001) were independently associated with osteoporosis in cirrhotic patients. Osteoporosis occurred in about one-fifth of patients with liver cirrhosis, which was associated with age, BMI, Fibroscan score, and alcohol liver disease related liver cirrhosis. Abbreviations: ALD = alcohol liver disease, BMD = bone mineral density, BMI = body mass index, CRP = C-reaction protein, DXA = dual-energy x-ray absorptiometry, HBV = hepatitis B virus, HCV = hepatitis C virus, IL-1 = interleukin-1, PBC = primary biliary cirrhosis, PSC = primary sclerosing cholangitis, TNF = tumor necrosis factor.
doi:10.1097/md.0000000000010645 pmid:29768330 pmcid:PMC5976349 fatcat:3v6d6qavcrffriu3tfzx7hwdke

Feasibility of Patient-Controlled Sleep with Dexmedetomidine in Treating Chronic Intractable Insomnia

Jian-Xiong An, John P Williams, Qi-Wu Fang, Yong Wang, Hui Liu, Le Shi, Wen-Hao Zhang
2020 Nature and Science of Sleep  
Patient-controlled analgesia (PCA) is an "on-demand" system which allows patients to self-administer intravenous medications in small bolus doses. Based on the principles of PCA, we developed Patient-Controlled Sleep (PCSL) for chronic intractable insomnia where the traditional analgesics in PCA were replaced with dexmedetomidine (Dex), an alpha-2 agonist widely used for premedication, sedation, anxiolysis and analgesia. The purpose of this study was to assess the feasibility of the new method
more » ... or the treatment of chronic intractable insomnia. Patients with chronic intractable insomnia undergoing PCSL (n=20) were evaluated with the Pittsburgh Sleep Quality Index (PSQI), Symptom Checklist 90 (SCL-90), Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD) before and after the treatment. The patient characteristics, overall outcomes and related side effects were also assessed. Fifteen patients completed the treatment protocol. The duration of PCSL varied from a few days to four months, and the dosage of Dex gradually decreased without eliciting signs or symptoms of tolerance or physical dependence. The sleep quality improvement occurred immediately after the therapy in 12/15 patients, and of which, 7/12 patients achieved continuously improved sleep quality in follow-up. PCSL with Dex might be a potential treatment for patients with chronic intractable insomnia. However, it is an off-label use, and the potential side effects of dexmedetomidine with long-term use needs further evaluation.
doi:10.2147/nss.s262991 pmid:33239930 pmcid:PMC7682602 fatcat:2psgkfxpwjdg7jcl3zx4wrpqfu

Increasing Oxidative Stress with Progressive Hyperlipidemia in Human: Relation between Malondialdehyde and Atherogenic Index

Rui-Li Yang, Yong-Hui Shi, Gang Hao, Wu Li, Guo-Wei Le
2008 Journal of clinical biochemistry and nutrition  
The aim of this study was to examine whether malondialdehyde (MDA) formation, a marker of oxidant stress, is altered in different stages of development of hyperlipidemia and whether it correlates with atherogenic index (AI), an important risk factor of atherosclerosis. Commercial kits were used to measure the levels of lipid profile and antioxidant status in the serum of 15 hyperlipidemic patients and 30 age and sex-matched normolipidemic subjects. The normolipidemic subjects were divided into
more » ... ower and higher lipid groups according to their blood lipid level. The activities of superoxide dismutase and glutathione peroxidase decreased in higher lipid group compared with lower lipid group, and were even lower in hyperlipidemic subjects. An increase in the levels of MDA, triglycerides, total cholesterol and LDL-C concentration were observed in higher lipid group, and even significantly increased in hyperlipidemic patients. A significant progressive decline in HDL-C concentration was found during hyperlipidemia evolution. There was a positive correlation between MDA and AI (r = 0.61, p<0.05). These data indicate that oxidative stress is an early event in the evolution of hyperlipidemia, and appropriate support for enhancing antioxidant supply in higher lipid subjects may help prevent the course of the disease.
doi:10.3164/jcbn.2008044 pmid:19015749 pmcid:PMC2581765 fatcat:iymuncshejbdtord5ldpsvxkfm

Does power hierarchy benefit or hurt team performance? The roles of hierarchical consistency and power struggle

Hao JI, Xiao-Yun XIE, Yong-Ping XIAO, Xiao-Le GAN, Wen FENG
2019 Acta Psychologica Sinica  
The roles of hierarchical consistency and power struggle JI Hao 1,2 ; XIE Xiao-Yun 2 ; XIAO Yong-Ping 3 ; GAN Xiao-Le 3 ; FENG Wen 2 ( 1 Business School, Ningbo University, Ningbo 315211, China) ( 2 School  ... 
doi:10.3724/sp.j.1041.2019.00366 fatcat:osg3523qczfvdd32dc65srniau
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