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The large random errors in Micro-Electro-Mechanical System (MEMS) gyros are one of the major factors that affect the precision of inertial navigation systems. Based on the indoor inertial navigation system, an improved wavelet threshold de-noising method was proposed and combined with a gradient radial basis function (RBF) neural network to better compensate errors. We analyzed the random errors in an MEMS gyroscope by using Allan variance, and introduced the traditional wavelet thresholddoi:10.3390/mi10090608 pmid:31540303 pmcid:PMC6780760 fatcat:ggxttbo6xzcrfcw6je4jofw3ku
more »... s. Then, we improved the methods and proposed a new threshold function. The new method can be used more effectively to detach white noise and drift error in the error model. Finally, the drift data was modeled and analyzed in combination with the RBF neural network. Experimental results indicate that the method is effective, and this is of great significance for improving the accuracy of indoor inertial navigation based on MEMS gyroscopes.
Sheng, T. Sun, H. Wu, and Z. ...doi:10.1109/tps.2013.2238255 fatcat:nfy7lmggd5bl5loxdxjvqohvby
Downloaded by [HKUST Library], [Guowei Zhou] at 01:26 21 November 2012 H. Huang et al. Downloaded by [HKUST Library], [Guowei Zhou] at 01:26 21 November 2012 ... Zhou , Jianhui Yang , Sheng Liu , Feng You & Xinming Lei (2013): Diversity of freeliving and symbiotic Symbiodinium in the coral reefs of Sanya, South China Sea, Marine Biology Research, 9:2, 117-128 ...doi:10.1080/17451000.2012.708045 fatcat:dtvr4t223zefbpspsf3dsaif6m
Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease in cloven-hoofed livestock that results in severe consequences for international trade, posing a great economic threat to agriculture. The FMDV infection antagonizes the host immune responses via different signaling pathways to achieve immune escape. Strategies to escape the cell immune system are key to effective infection and pathogenesis. This review is focused on summarizing the recent advances to understanddoi:10.3390/pathogens9090729 pmid:32899635 fatcat:uhxy3hq5vjdefi6wrmlr6tktfm
more »... the proteins encoded by FMDV antagonize the host innate and adaptive immune responses.
BMC Plant Biology
Posttranslational modification of proteins by small ubiquitin like modifier (SUMO) proteins play an important role during the developmental process and in response to abiotic stresses in plants. However, little is known about SUMOylation in peanut (Arachis hypogaea L.), one of the world's major food legume crops. In this study, we characterized the SUMOylation system from the diploid progenitor genomes of peanut, Arachis duranensis (AA) and Arachis ipaensis (BB). Genome-wide analysis revealeddoi:10.1186/s12870-019-2136-9 pmid:31884953 fatcat:r4plfchvvnd6ne6ytegfrweo44
more »... e presence of 40 SUMO system genes in A. duranensis and A. ipaensis. Our results showed that peanut also encodes a novel class II isotype of the SCE1, which was previously reported to be uniquely present in cereals. RNA-seq data showed that the core components of the SUMOylation cascade SUMO1/2 and SCE1 genes exhibited pod-specific expression patterns, implying coordinated regulation during pod development. Furthermore, both transcripts and conjugate profiles revealed that SUMOylation has significant roles during the pod development. Moreover, dynamic changes in the SUMO conjugates were observed in response to abiotic stresses. The identification and organization of peanut SUMO system revealed SUMOylation has important roles during stress defense and pod development. The present study will serve as a resource for providing new strategies to enhance agronomic yield and reveal the mechanism of peanut pod development.
Hepatocellular carcinoma (HCC), the most common primary tumor of the liver, has a poor prognosis, rapid progression. The aim of the current study was to establish a stable lentiviral expression vector for enhanced green fluorescent protein (EGFP) and to evaluate biological characteristics on HCC growth and migration following transfection of HCC cells with EGFP. McA-RH7777 cells were transfected with EGFP overexpression lentiviral vector. Cell activity and mobility were monitored with a Cell-IQdoi:10.3892/etm.2018.6580 pmid:30250518 pmcid:PMC6145064 fatcat:si53bzd3u5czjc6kb6ljn4bgri
more »... Analyzer. Transwell assays were performed to detect invasiveness and flow cytometry was performed for cell cycle analysis. A subcutaneous tumor rat model was established to analyze the stability of fluorescent protein expression. The result suggested no significant differences between wild-type and EGFP-overexpressing McA-RH7777 cells with regards to cell proliferation, activity, mobility, invasiveness and cell cycle. Green fluorescence was detected over 108 days of culturing. The subcutaneous tumor rat model demonstrated that EGFP expression had no influence on tumor growth and long-term expression was stable. The stable EGFP expression of the HCC transplanted tumor rat model may share biological characteristics with human liver cancer. The model established in the current study may be suitable for various applications, including research focusing on liver cancer metastasis and recurrence, interventional therapy, imaging diagnosis and drug screenings.
Copyright: Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT We aim to investigate the safety and efficacy of microwave ablation (MWA) combined with attenuated Salmonella typhimurium strain VNP20009 in treating hepatocellular carcinoma. Portions of tumor tissues weredoi:10.18632/oncotarget.17468 pmid:28498813 pmcid:PMC5564595 fatcat:eyfrji6rhfhwllwvr4nrb7kle4
more »... cally implanted in the livers of 40 male rats weighed 150~200 g to establish tumor models. Three weeks later, the rats were randomly divided into four groups: (A) MWA plus VNP20009 group; (B) MWA group; (C) VNP20009 group; and (D) control group. Incomplete MWA was performed (20~30 W, 1~2 min) after the hepatic carcinoma was properly exposed. VNP20009 (about 1×10 7 cfu) was directly injected into the tumor immediately. MRI scans were performed to assess the tumor responses 7 and 14 days later, respectively. Micro CT was used to observe the lung metastases. After the animals were sacrificed or died, the tumors were cut off for the purpose of pathological and immunohistochemical analyses. The results showed that the mean tumor volumes of MWA plus VNP20009 group on the 7th and 14th day post treatment were obviously smaller than those of other groups (P < 0.05). Lung metastases rates were 20%, 60%, 30% and 100% in MWA plus VNP20009 group, MWA group, VNP20009 group and control group, respectively. The median survival of the rats in MWA plus VNP20009 group was distinctly longer than those in other groups (P < 0.05). In summary, MWA combined with VNP20009 produced better effects than MWA or VNP20009 alone in treating hepatic carcinoma. This strategy might have potential ability to decrease lung metastases and prolong the overall survival.
Coral reefs are significant ecosystems. The ecological success of coral reefs relies on not only coral-algal symbiosis but also coral-microbial partnership. However, microbiome assemblages in the South China Sea corals remain largely unexplored. Here, we compared the microbiome assemblages of reef-building corals Galaxea (G. fascicularis) and Montipora (M. venosa, M. peltiformis, M. monasteriata) collected from five different locations in the South China Sea using massively-parallel sequencingdoi:10.1038/s41598-018-20515-w pmid:29402898 pmcid:PMC5799258 fatcat:gtizuh67uzdajjhtxkthgzuhuq
more »... f 16S rRNA gene and multivariate analysis. The results indicated that microbiome assemblages for each coral species were unique regardless of location and were different from the corresponding seawater. Host type appeared to drive the coral microbiome assemblages rather than location and seawater. Network analysis was employed to explore coral microbiome co-occurrence patterns, which revealed 61 and 80 co-occurring microbial species assembling the Galaxea and Montipora microbiomes, respectively. Most of these co-occurring microbial species were commonly found in corals and were inferred to play potential roles in host nutrient metabolism; carbon, nitrogen, sulfur cycles; host detoxification; and climate change. These findings suggest that the co-occurring microbial species explored might be essential to maintain the critical coral-microbial partnership. The present study provides new insights into coral microbiome assemblages in the South China Sea. Coral reef ecosystems are considered as the tropical rainforests of the sea, nurturing the highest biodiversity of marine life and providing vital ecosystem goods and services 1,2 . However, coral reefs around the world have suffered from declines and extinction risks largely due to bleaching events and emerging/reemerging diseases induced by climate change and anthropogenic disturbances 3,4 . The ecological success of coral reefs relies on coral-algal symbiosis, and recent studies using 16S rRNA gene amplicon pyrosequencing have revealed highly diverse and abundant microbes in individual coral colonies 5-9 . It is believed that some of these microbes can form partnerships with coral hosts and help them with possible access to those unavailable nutrients and metabolic pathways 10
Increasing evidence shows that circular RNAs (circRNAs) play a regulatory role in cancer. In the present study, we aimed to investigate the characteristics and effects of hsa_circ_0026134 in hepatocellular carcinoma (HCC). We investigated hsa_circ_0026134 expression in 20 pairs of clinical tissues from HCC patients; expression of hsa_circ_0026134 in different cell lines; effect of hsa_circ_0026134 on proliferation and invasion of HCC cell lines; and the regulatory mechanisms and interactionsdoi:10.1042/bsr20191418 fatcat:ygya6uctefd7rczgdbkartxcbe
more »... ng hsa_circ_0026134, miR-127-5p, tripartite motif-containing protein 25 (TRIM25) and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3). hsa_circ_0026134 expression was increased in HCC samples and cell lines. Downregulation of hsa_circ_0026134 attenuated HCC cell proliferation and metastatic properties. Micro (mi)RNA (miR)-127-5p was sponged by hsa_circ_0026134. Rescue experiments indicated that inhibition of miR-127-5p expression promoted cell proliferation and invasion even after hsa_circ_0026134 silencing. TRIM25 and IGF2BP3 were targets of miR-127-5p. Overexpression of TRIM25 or IGF2BP3 promoted cell proliferation and invasion in cells overexpressing miR-127-5p. Downregulation of hsa_circ_0026134 suppressed TRIM25- and IGF2BP3-mediated HCC cell proliferation and invasion via promotion of miR-127-5p expression, which have been confirmed by luciferase reporter assay. This study provides a new treatment target for HCC.
Coral reefs are ecologically significant habitats. Coral-algal symbiosis confers ecological success on coral reefs and coral-microbial symbiosis is also vital to coral reefs. However, current understanding of coral-microbial symbiosis on a genomic scale is largely unknown. Here we report a potential microbial symbiont in corals revealed by metagenomics-based genomic study. Microbial cells in coral were enriched for metagenomic analysis and a high-quality draft genome of "Candidatusdoi:10.1038/s41598-017-09032-4 pmid:28839161 pmcid:PMC5571212 fatcat:bya2m3yyyngwbd7z2nh6neqrdi
more »... ris korallensis" was recovered by metagenome assembly and genome binning. Phylogenetic analysis shows "Ca. P. korallensis" belongs to the Prosthecochloris clade and is clustered with two Prosthecochloris clones derived from Caribbean corals. Genomic analysis reveals "Ca. P. korallensis" has potentially important ecological functions including anoxygenic photosynthesis, carbon fixation via the reductive tricarboxylic acid (rTCA) cycle, nitrogen fixation, and sulfur oxidization. Core metabolic pathway analysis suggests "Ca. P. korallensis" is a green sulfur bacterium capable of photoautotrophy or mixotrophy. Potential host-microbial interaction reveals a symbiotic relationship: "Ca. P. korallensis" might provide organic and nitrogenous nutrients to its host and detoxify sulfide for the host; the host might provide "Ca. P. korallensis" with an anaerobic environment for survival, carbon dioxide and acetate for growth, and hydrogen sulfide as an electron donor for photosynthesis.
The success of coral reef ecosystems largely depends on mutualistic symbiosis between scleractinian corals and the dinoflagellate photosymbiont Symbiodinium spp. However, further investigation is needed to elucidate the flexibility of coral-algae associations in response to environmental changes. In this study, we applied a molecular method (high-throughput internal transcribed spacer 2 region of ribosomal RNA gene amplicon sequencing) to explore diversity and flexibility of Symbiodiniumdoi:10.3389/fmicb.2017.02487 pmid:29312196 pmcid:PMC5733085 fatcat:5lsl57y7yjdcdbuh7drx5iu34q
more »... ted with Galaxea fascicularis, an ecologically important scleractinian coral species collected at five locations around Hainan Island, South China Sea. The results revealed a high diversity of Symbiodinium subclades with C2r and D17 being dominant in G. fascicularis. Clade D Symbiodinium occurred most frequently in habitats where the annual average sea surface temperatures are the highest, suggesting that temperature is an important factor in determining Symbiodinium D abundance in G. fascicularis. The distribution of coral-Symbiodinium associations are possibly mediated by trade-off mechanisms which change the relative abundance of Symbiodinium clades/subclades under different environmental conditions. These findings provide further evidence that reef-building corals such as G. fascicularis can shuffle their symbionts to cope with environmental changes, and have implications for our understanding of the ecology of flexible coral-algal symbiosis.
To retrospectively evaluate the safety and efficacy of ultrasound-guided percutaneous microwave ablation (MWA) combined with simultaneous transarterial chemoembolization (TACE) in the treatment of patients with advanced intrahepatic cholangiocarcinoma (ICC). All patients treated with ultrasound-guided percutaneous MWA combined with simultaneous TACE for advanced ICC at our institution were included. Posttreatment contrast-enhanced computed tomography and/or magnetic resonance imaging weredoi:10.2147/ott.s84764 pmid:26060410 pmcid:PMC4454214 fatcat:tawrvxmbw5du5lxoyhwpdbzn7a
more »... ved and reviewed for tumor response to the treatment. Routine laboratory studies, including hematology and liver function tests were collected and analyzed. Procedure-related complications were reviewed and survival rates were analyzed. From January 2011 to December 2014, a total of 26 advanced ICC patients were treated at our single institute with ultrasound-guided percutaneous MWA combined with simultaneous TACE. There were 15 males and eleven females with an average age of 57.9±10.4 years (range, 43-75 years). Of 26 patients, 20 (76.9%) patients were newly diagnosed advanced ICC without any treatment, and six (23.1%) were recurrent and treated with surgical resection of the original tumor. The complete ablation rate was 92.3% (36/39 lesions) for advanced ICC. There were no major complications observed. There was no death directly from the treatment. Median progression-free survival and overall survival were 6.2 and 19.5 months, respectively. The 6-, 12-, and 24-month survival rates were 88.5%, 69.2%, and 61.5%, respectively. The study suggests that ultrasound-guided percutaneous MWA combined with simultaneous TACE therapy can be performed safely in all patients with advanced ICC. The complete ablation rate was high and there was no major complication. The overall 24-month survival was 61.5%.
With the increasing anthropogenic CO 2 concentration, ocean acidification (OA) can have dramatic effects on coral reefs. However, the effects of OA on coral physiology and the associated microbes remain largely unknown. In the present study, reef-building coral Acropora gemmifera collected from a reef flat with highly fluctuating environmental condition in the South China Sea were exposed to three levels of partial pressure of carbon dioxide (pCO 2 ) (i.e., 421, 923, and 2070 μatm) for fourdoi:10.1038/srep35971 pmid:27786309 pmcid:PMC5082368 fatcat:wu6yozrehjgllj5bazpgjjzmcq
more »... s. The microbial community structures associated with A. gemmifera under these treatments were analyzed using 16S rRNA gene barcode sequencing. The results revealed that the microbial community associated with A. gemmifera was highly diverse at the genus level and dominated by Alphaproteobacteria. More importantly, the microbial community structure remained rather stable under different pCO 2 treatments. Photosynthesis and calcification in A. gemmifera, as indicated by enrichment of δ 18 O and increased depletion of δ 13 C in the coral skeleton, were significantly impaired only at the high pCO 2 (2070 μatm). These results suggest that A. gemmifera can maintain a high degree of stable microbial communities despite of significant physiological changes in response to extremely high pCO 2 . Rising CO 2 in the atmosphere elevates the partial pressure of carbon dioxide (pCO 2 ) in seawater and reduces the global oceanic pH and carbonate ion concentrations, which is called ocean acidification (OA). It has been suggested that OA has profound effects on marine organisms and ecosystems, particularly calcifying organisms such as reef-building corals 1-3 . With the increasing OA associated with ocean warming due to the rising global CO 2 emissions 2 , there is an urgent need to understand and predict the tolerance and response of corals to future climate change. Reef-building corals are commonly referred to as holobionts, which comprise coral host and associated microorganisms including endosymbiotic photosynthetic algae, bacteria, and archaea, among others. These complex microbial partners play pivotal roles in coral health and holobiont function in carbon, nitrogen and sulfur cycles. The future fate of coral reefs largely depends on the capacity of corals and their symbionts to acclimatize or adapt to climate change 2,4-7 . There is emerging evidence that corals can adapt to climate change 8 , although coral photosynthesis and growth can be negatively impacted by OA 9 . Moreover, flexible coral-algal symbiosis may facilitate the acclimatization/adaptation of the holobiont through algal shuffling or switching 10, 11 . Shifting in the composition of coral-associated microbiota has been observed following environmental disturbances (e.g., elevated temperature) and has often been linked to impaired host health 12-14 , although it has also been hypothesized to mediate holobiont resistance to environmental perturbations 6,15 . Coral-associated microbial communities may be affected directly or indirectly by OA and would subsequently compromise holobiont fitness (i.e., changes in photosynthesis or calcification) and survival, possibly due to a shift in the functional roles of microbial associations 12,16-18 . However, to date it is not clear how microbial communities change in coral in
Edited by Amanda Fosang In the three-dimensional extracellular matrix of the insect cuticle, horizontally aligned microfibrils composed of the polysaccharide chitin and associated proteins are stacked either parallel to each other or helicoidally. The underlying molecular mechanisms that implement differential chitin organization are largely unknown. To learn more about cuticle organization, we sought to study the role of chitin deacetylases (CDA) in this process. In the body cuticle of nymphsdoi:10.1074/jbc.m116.720581 pmid:27637332 pmcid:PMC5114393 fatcat:vnq24w6dbngufjakgx4l6abpvm
more »... f the migratory locust Locusta migratoria, helicoidal chitin organization is changed to an organization with unidirectional microfibril orientation when LmCDA2 expression is knocked down by RNA interference. In addition, the LmCDA2-deficient cuticle is less compact suggesting that LmCDA2 is needed for chitin packaging. Animals with reduced LmCDA2 activity die at molting, underlining that correct chitin organization is essential for survival. Interestingly, we find that LmCDA2 localizes only to the initially produced chitin microfibrils that constitute the apical site of the chitin stack. Based on our data, we hypothesize that LmCDA2mediated chitin deacetylation at the beginning of chitin production is a decisive reaction that triggers helicoidal arrangement of subsequently assembled chitin-protein microfibrils. In the 60s and 70s of the last century, Neville (1) pioneered studies of chitin organization in the cuticle of insects. He elegantly described chitin organization levels by ultrastructural analyses using especially the migratory locust Locusta migratoria as a model insect. Chitin, the polymer of ␤-1,4-linked N-acetylglucosamine residues, interacts with proteins to form microfibrils that are aligned in parallel constituting horizontal sheets (laminae). These sheets are stacked either helicoidally or with unidirectional microfibril orientation along the vertical axis of the cuticle (2). In the locust tibia, helicoidal and nonhelicoidal laminar organization alternate periodically reflecting different mechanisms of chitin production and orientation (2) . This organization might have a yet unexplored impact on the mechanical properties of the cuticle. Cuticular pore canals connecting the cell surface with the cuticle surface follow chitin orientation; in helicoidally arranged laminae, they are crescentic (a term used by Neville), but in regions of non-helicoidal laminae they are straight (3, 4). Conceivably, chitin organization depends on cuticle proteins that interact with chitin. In a few recent studies, it was demonstrated that so-called CPR (cuticular protein with a Rebers and Riddiford consensus domain) proteins determine hardness in the elytra of the red flour beetle Tribolium castaneum (5, 6). Neville added another level of complexity to the question of cuticle organization. It had been observed that every fifth or sixth sugar residue in chitin is deacetylated (7). He speculated that chitin and the partly deacetylated form of chitin, called chitosan, might be recognized by different sets of yet unidentified proteins. Chitin deacetylation is not a spontaneous event but requires the activity of chitin deacetylases (CDAs) 4 that belong to the carbohydrate esterase family 4 (CE-4) (8). Recent phylogenetic analyses of CDA sequences from four insect species showed that insect CDAs can be classified into five groups based on sequence similarity and domain diversity (9). Group I includes CDA1 and CDA2 that have a chitin-binding peritrophin-A domain (ChBD), a low-density lipoprotein receptor class A domain (LDLa), and a polysaccharide deacetylase-like catalytic domain. Group II is composed of CDA3 that has the same domain composition as members of group I CDAs. The overall sequence similarity is, however, as low as 38%. Groups III and IV include CDA4 and CDA5, respectively, which along with the deacetylase signature have a ChBD but no
Lin Yang, Na Wei, Guowei Shang, and Yanhui Ji acquired and analyzed the data. Songfeng Chen, Sitao Lu, and Hongwei Kou conceived the study and wrote the manuscript. ...doi:10.1155/2020/6976317 pmid:33376733 pmcid:PMC7738793 fatcat:p3kql4twq5borkvrt6vtx7zpva
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