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Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images

L Kubínová, J Janácek, P Karen, B Radochová, F Difato, I Krekule
2004 Physiological Research  
Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological  ...  One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described.  ...  Schwarzerová (Department of Plant Physiology, Faculty of Science, Charles University, Prague) for preparing the specimen of tobacco cells shown in Figure 1  ... 
pmid:15119935 fatcat:h4ixjzdemjf2zpk2eyukmlaz5e

Retinal topography maps in R: New tools for the analysis and visualization of spatial retinal data

Brian A. Cohn, Shaun P. Collin, Peter C. Wainwright, Lars Schmitz
2015 Journal of Vision  
Retina users can compute retinal topography maps independent of stereology software choice and assess model fits with a variety of diagnostic plots.  ...  Here, we introduce Retina, an R package for computational mapping, inspection of topographic model fits, and generation of average maps.  ...  (A) Simplified retinal wholemount with superimposed stereology sampling grid. The sampling locations are at the intersections of the uniform grid.  ... 
doi:10.1167/15.9.19 pmid:26230981 pmcid:PMC4527213 fatcat:gwd5xmwm6bayrd6vgiapxc3kje

Advantages and pitfalls of using free-hand sections of frozen needles for three-dimensional analysis of mesophyll by stereology and confocal microscopy

Z. LHOTÁKOVÁ, J. ALBRECHTOVÁ, J. JANÁČEK, L. KUBÍNOVÁ
2008 Journal of Microscopy  
An efficient design-based stereological method can be applied for estimation of this parameter, using software-randomized virtual fakir test probes in stacks of optical sections acquired by a confocal  ...  Confocal microscopy, fakir method, internal surface area, mesophyll, Norway spruce, stereology.  ...  In the present study, we suggest to solve this problem by freezing the needles and storing them for later analysis.  ... 
doi:10.1111/j.1365-2818.2008.02079.x pmid:19017201 pmcid:PMC2657997 fatcat:sntfl4boj5gvlnxaos3qoo7oy4

DEVELOPMENT OF A COMPUTER SYSTEM FOR IDENTITY AUTHENTICATION USING ARTIFICIAL NEURAL NETWORKS

Timur Kartbayev, Bahitzhan Akhmetov, Aliya Doszhanova, Kaiyrkhan Mukapil, Aliya Kalizhanova, Gulnaz Nabiyeva, Lyazzat Balgabayeva, Feruza Malikova
2017 Image Analysis and Stereology  
The structural scheme of intelligent system for identification based on artificial neural networks is proposed in this work.  ...  On the basis of expert knowledge, the fuzzy base of products is designed. It allows assessing possible changes in key characteristics, used to authenticate identity based on the image.  ...  The solution may serve as a basis for the hardware implementation of the proposed neuro-fuzzy algorithm when solving the problems of identity authentication in terms of hardware and software complex of  ... 
doi:10.5566/ias.1612 fatcat:tp6qywykb5bz5kox2ai47lsffq

Current automated 3D cell detection methods are not a suitable replacement for manual stereologic cell counting

Christoph Schmitz, Brian S. Eastwood, Susan J. Tappan, Jack R. Glaser, Daniel A. Peterson, Patrick R. Hof
2014 Frontiers in Neuroanatomy  
Thus, at present, stereologic cell counting with manual decision for object inclusion according to unbiased stereologic counting rules remains the only adequate method for unbiased cell quantification  ...  Stereologic cell counting has had a major impact on the field of neuroscience.  ...  Because of its simple model of cell appearance, this algorithm suffers from several problems when used outside Frontiers in Neuroanatomy www.frontiersin.org of very tightly controlled environments.  ... 
doi:10.3389/fnana.2014.00027 pmid:24847213 pmcid:PMC4019880 fatcat:epvcq2ec5fcmfbuhjumnqeczkq

MULTISCALE IMAGE ANALYSIS BASED ON ROBUST AND ADAPTIVE MORPHOLOGICAL SCALE-SPACES

El Hadji Samba Diop, Jesus Angulo
2014 Image Analysis and Stereology  
Accurate numerical schemes are also provided to solve proposed PDEs, and experiments conducted for both synthetic and real images, show the efficiency and robustness of our approach.  ...  Dealing with partial differential equations (PDEs) for generalized Cauchy problems, we show that proposed PDEs are equivalent to impose robustness and adaptability of corresponding sup-inf operators, to  ...  Generalized Cauchy problems for continuous multiscale models.  ... 
doi:10.5566/ias.993 fatcat:66gvwnhmcveenaazsw5fezifae

Evolutionary Black-box Topology Optimization: Challenges and Promises

David Guirguis, Carlos Artemio Coello Coello, Kazuhiro Saitou, Nikola Aulig, Renato Picelli, Bo Zhu, Yuqing Zhou, William Vicente, Francesco Iorio, Markus Olhofer, Wojciech Matusik
2019 IEEE Transactions on Evolutionary Computation  
Consequently, future research in this area may open the door for innovating new applications in science and engineering that may go beyond solving classical optimization problems of structural engineering  ...  Furthermore, algorithms designed for BBTO can be added to existing software toolboxes and packages of topology optimization.  ...  High-performance solvers [280] are combined with an octree grid to solve large-scale problems with adaptivity.  ... 
doi:10.1109/tevc.2019.2954411 fatcat:v72p6dsrvffmvimb3ajeifxskm

Variation in Brain Regions Associated with Fear and Learning in Contrasting Climates

Timothy C. Roth, II, Caitlin M. Gallagher, Lara D. LaDage, Vladimir V. Pravosudov
2012 Brain, Behavior and Evolution  
Introduction Behavioral flexibility allows for the expression of different behavioral outcomes based on previous experiences [Dukas, 1998; Reader, 2003] , and such flexibility seems to be adaptive, especially  ...  Overall, we provide some evidence that populationrelated differences in problem solving and neophobia may be associated with differences in volume and neuron numbers of our target brain regions.  ...  Ream for their assistance in the field. We thank G. Hanson and L. Chancellor for their assistance in the laboratory. We are also thankful for advice from N. Clayton, D. Gammon, K.  ... 
doi:10.1159/000335421 pmid:22286546 pmcid:PMC3343761 fatcat:7j3enjcge5gozfpx2awxfkresq

Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology

Zuzana Burdíková, Martin Čapek, Pavel Ostašov, Jiří Machač, Radek Pelc, Edward A.D. Mitchell, Lucie Kubínová
2010 Microscopy and Microanalysis  
Testate amoebae taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or~environmental! scanning electron microscopy~SEM/ESEM!.  ...  visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems.  ...  , either in SEM or environmental SEM~ESEM! mode. For the SEM~high-vacuum!  ... 
doi:10.1017/s1431927610094031 pmid:21092357 fatcat:gl3ldbldn5effgfgqjwayxvp3e

Elements for an Integrated Geostatistical Modeling of Heterogeneous Reservoirs

L. Y. Hu, M. Le Ravalec-Dupin
2004 Oil & Gas Science and Technology  
Building a geostatistical reservoir model must account for the geological depositional environment of the reservoir, so as to represent the major heterogeneities that control fluid flow.  ...  This is followed by an outline on the inference of the model structural parameters. Then, we present an inverse approach based on the gradual deformation method for history matching.  ...  This can be performed using the finite difference method which requires solving the forward problem twice for each t.  ... 
doi:10.2516/ogst:2004011 fatcat:bbrgvrb7mnd6jov6uebj2t43b4

Advances in rheology and flow assurance studies of waxy crude

Jinjun Zhang, Bo Yu, Hongying Li, Qiyu Huang
2013 Petroleum Science  
Therefore, we introduced the reliability-based method to solve this problem.  ...  By using this model to solve the steady problems, calculation may be speeded up by hundreds or even thousands of times, and even for unsteady problems calculation may be accelerated by dozens of times  ... 
doi:10.1007/s12182-013-0305-2 fatcat:tu4q5yfdqvhwvbskpxk4lgqa2q

The relationship between the structural and orthogonal compressive properties of trabecular bone

R.W. Goulet, S.A. Goldstein, M.J. Ciarelli, J.L. Kuhn, M.B. Brown, L.A. Feldkamp
1994 Journal of Biomechanics  
Regression analysis was used to estimate the modulus and ultimate strength; these regressions accounted for 68-90% of the variance in these measures.  ...  A high correlation between the basic stereologic measurements was found, indicating that there is a relationship between the amount of bone and number of trabeculae in cancellous bone.  ...  By solving the eigenvalue problem for this tensor, the principal structural directions and magnitudes are obtained.  ... 
doi:10.1016/0021-9290(94)90014-0 pmid:8188719 fatcat:7pdkransljc2pkatpm6c6jwcuu

Microstructural Analysis of Powder Metallurgy Tool Steels in the Context of Abrasive Wear Behavior: A New Computerized Approach to Stereology

Santiago Benito, Nils Wulbieter, Fabian Pöhl, Werner Theisen
2019 Journal of materials engineering and performance (Print)  
All three aforementioned stages contain novel contributions that can be potentially beneficial to the field of materials design in general and to the field of alloy design for severely abrasive environments  ...  Models of known geometry and micrographs of different powder metallurgy steels were analyzed, and the obtained results were compared with the obtained by the linear intercept method.  ...  This means that it is not possible to strive for the perfect wear-resistant material in all possible work environments: Each problem must be analyzed and solved independently.  ... 
doi:10.1007/s11665-019-04036-9 fatcat:43h47btqfndh3bhxgfxufxpxjq

Electrical Structure of Biological Cells and Tissues: impedance spectroscopy, stereology, and singular perturbation theory [article]

Robert Eisenberg
2015 arXiv   pre-print
Impedance spectroscopy combined with appropriate structural knowledge provides insight into pathways for current flow, with more success than other methods.  ...  Many tissues are riddled with extracellular space as clefts and tubules, for example, cardiac muscle, the lens of the eye, most epithelia, and of course frog muscle.  ...  Stereology depends on the projection of an image onto a grid (usually rectangular) of LOW resolution with spacing between lines much larger than one might guess, and very much larger than that necessary  ... 
arXiv:1511.01339v1 fatcat:j4ybxbcmlffxtbes6wpj3lj4vq

Index—Volumes 1–89

1997 Artificial Intelligence  
technique 283 stereology 283 mathematical principle 283 stereopsis human -202 differential equation model 1280 domains 1239 modeling 1244 modeling of genetic algorithms 1244 models for genetic  ...  -120 problem solving robot -83 routine -342 scientific-237 search 72,407 problem solving situation 3, 110 complex -3 problem solving strategy 503 problem solving system 154,323, 519,641, 1306  ...  machinery 58 1 management of evidential -328 mathematical -166,579 mathematics of non-monotonic-150 mechanism 381 mechanized formal -152 meta-level -185 method geometric -12 17 methods choices of -1157 models  ... 
doi:10.1016/s0004-3702(97)80122-1 fatcat:6az7xycuifaerl7kmv7l3x6rpm
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