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Observations upon the standardisation of bacterial vaccines by the Wright, the hæmocytometer, and the plate culture methods

Ernest Glynn, Mildred Powell, A. Armstrong Rees, G. Lissand Cox
1913 The Journal of Pathology and Bacteriology  
Wright. Percentage Increase in Emulsion standardised by 0'02 Well above Wright.  ...  Wright (1902 z, first described his method in 1902.  ...  I n order to ascertain whether the Wright film or the 0.02 hzmocytometer chamber was more accumte, two observers standardised the same emulsion by each method, and took as the practical test of accuracy  ... 
doi:10.1002/path.1700180139 fatcat:u54bi6gog5drvgqtanxrbfxm2y

Video-rate photometric stereo-imaging with general lighting luminaires

Johannes Herrnsdorf, Laurence Broadbent, Glynn C. Wright, Martin D. Dawson, Michael J. Strain
2017 2017 IEEE Photonics Conference (IPC)  
3D images of moving objects can be achieved with a surveillance camera and four white light-emitting diodes. With these simple components, an imaging rate of 15 Hz is possible, limited by the camera framerate.
doi:10.1109/ipcon.2017.8116194 fatcat:mkpenzubxnfddpnej3o44b3igi

Statin Adherence and Risk of Accidents: A Cautionary Tale

C. R. Dormuth, A. R. Patrick, W. H. Shrank, J. M. Wright, R. J. Glynn, J. Sutherland, M. A. Brookhart
2009 Circulation  
Dr Glynn has received past grant support from AstraZeneca and Bristol-Myers Squibb. The remaining authors report no conflicts.  ...  Dr Brookhart was partly supported by a career development award from the National Institutes of Health (AG-027400), and Dr Glynn was partly supported by a National Institutes of Health grant (AG-018833  ... 
doi:10.1161/circulationaha.108.824151 pmid:19349320 pmcid:PMC2744446 fatcat:5ydgz6gbfrbk5drko6ujh4nwuq

LED-Based Photometric Stereo-Imaging Employing Frequency-Division Multiple Access

Johannes Herrnsdorf, Jonathan McKendry, Mark Stonehouse, Laurence Broadbent, Glynn C. Wright, Martin D. Dawson, Michael J. Strain
2018 2018 IEEE Photonics Conference (IPC)  
2018) LED-based photometric stereo-imaging employing frequency-division multiple access. In: 31st IEEE Photonics Conference, IPC 2018. IEEE, Piscataway, NJ. ISBN 9781538653586 , http://dx.Abstract-We present a photometric stereo-imaging approach based on illumination with light-emitting diodes (LEDs) from different angles where the LEDs are sinusoidally modulated and do not require synchronization with each other or with the camera.
doi:10.1109/ipcon.2018.8527181 fatcat:77db65amcvhi7egvlvflaplm5m

Hepcidin level predicts hemoglobin concentration in individuals undergoing repeated phlebotomy

A. E. Mast, K. S. Schlumpf, D. J. Wright, B. Johnson, S. A. Glynn, M. P. Busch, G. Olbina, M. Westerman, E. Nemeth, T. Ganz
2013 Haematologica  
Dietary iron absorption is regulated by hepcidin, an iron regulatory protein produced by the liver. Hepcidin production is regulated by iron stores, erythropoiesis and inflammation, but its physiology when repeated blood loss occurs has not been characterized. Hepcidin was assayed in plasma samples obtained from 114 first-time/reactivated (no blood donations in preceding 2 years) female donors and 34 frequent (≥3 red blood cell donations in preceding 12 months) male donors as they were
more » ... ized ≥4 times over 18-24 months. Hepcidin levels were compared to ferritin and hemoglobin levels using multivariable repeated measures regression models. Hepcidin, ferritin and hemoglobin levels declined with increasing frequency of donation in the first-time/reactivated females. Hepcidin and ferritin levels correlated well with each other (Spearman's correlation of 0.74), but on average hepcidin varied more between donations for a given donor relative to ferritin. In a multivariable repeated measures regression model the predicted inter-donation decline in hemoglobin varied as a function of hepcidin and ferritin; hemoglobin was 0.51 g/dL lower for subjects with low (≤45.7 ng/mL) or decreasing hepcidin and low ferritin (≤26 ng/mL), and was essentially zero for other subjects including those with high (>45.7 ng/mL) or increasing hepcidin and low ferritin (≤26 ng/mL) levels (P<0.001). In conclusion, hepcidin levels change rapidly in response to dietary iron needed for erythropoiesis. The dynamic regulation of hepcidin in the presence of a low levels of ferritin suggests that plasma hepcidin concentration may provide clinically useful information about an individual's iron status (and hence capacity to tolerate repeated blood donations) beyond that of ferritin alone. identifier: NCT00097006 ABSTRACT © F e r r a t a S t o r t i F o u n d a t i o n N o c o m m e r c i a l u s e
doi:10.3324/haematol.2012.070979 pmid:23445875 pmcid:PMC3729915 fatcat:ejudl6fp2jfovoqsgumelng6h4

The effect of COVID-19 public health measures on nationally notifiable diseases in Australia: preliminary analysis

Amy Bright, Anna-Jane Glynn-Robinson, Stacey Kane, Rose Wright, Nathan Saul
2020 Communicable Diseases Intelligence  
1 Ms Stacey Kane 1 Ms Rose Wright 1 Dr Nathan Saul 1 1.  ...  unspecified duration 1,242 1,417 1,088 14% 30% ) . ) Commun Dis Intell (2018) 2020;44 ( Epub 5/11/2020 Ms Amy Bright 1 Ms Anna-Jane Glynn-Robinson  ... 
doi:10.33321/cdi.2020.44.85 pmid:33147428 fatcat:3tf3h32kffey3l65ezbs4q73um

Lighting as a Service That Provides Simultaneous 3D Imaging and Optical Wireless Connectivity

Johannes Herrnsdorf, Jonathan McKendry, Mark Stonehouse, Laurence Broadbent, Glynn C. Wright, Martin D. Dawson, Michael J. Strain
2018 2018 IEEE Photonics Conference (IPC)  
2018) Lighting as a service that provides simultaneous 3D imaging and optical wireless connectivity. In: 2018 IEEE Photonics Conference. IEEE, Piscataway, NJ. ISBN 9781538653586 , http://dx. Abstract-Light-emitting diodes enable optical wireless data transmission and advanced imaging methods such as photometric stereo-imaging. Both, wireless communications into a scene and 3D imaging of that scene is enabled in parallel using the same set of LEDs thus providing lighting-based infrastructure e.g. for automated agents.
doi:10.1109/ipcon.2018.8527294 fatcat:4ve3j6sl25bt7pyayk7fqw3poe

Broad-Leaf Weed Detection in Pasture

Wenhao Zhang, Mark F. Hansen, Timothy N. Volonakis, Melvyn Smith, Lyndon Smith, Jim Wilson, Graham Ralston, Laurence Broadbent, Glynn Wright
2018 2018 IEEE 3rd International Conference on Image, Vision and Computing (ICIVC)  
Weed control in pasture is a challenging problem that can be expensive and environmentally unfriendly. This paper proposes a novel method for recognition of broad-leaf weeds in pasture such that precision weed control can be achieved with reduced herbicide use. Both conventional machine learning algorithms and deep learning methods have been explored and compared to achieve high detection accuracy and robustness in real-world environments. In-pasture grass/weed image data have been captured for
more » ... classifier training and algorithm validation. The proposed deep learning method has achieved 96.88% accuracy and is capable of detecting weeds in different pastures under various representative outdoor lighting conditions.
doi:10.1109/icivc.2018.8492831 fatcat:qlswf4pr45dwbbzhrxnoqouqru

Supplementary document for Synchronization-Free Top-Down Illumination Photometric Stereo Imaging Using Light-Emitting Diodes and a Mobile Device - 4962329.pdf

Emma Le Francois, Johannes Herrnsdorf, Jonathan McKendry, Laurence Broadbent, Glynn Wright, Martin D. Dawson, Michael Strain
Supplement 1
doi:10.6084/m9.figshare.13298588.v2 fatcat:rsqkxpydmzaz3oheh7dibbbmp4

Potential impact on blood availability and donor iron status of changes to donor hemoglobin cutoff and interdonation intervals

Bryan R. Spencer, Bryce Johnson, David J. Wright, Steven Kleinman, Simone A. Glynn, Ritchard G. Cable
2016 Transfusion  
BACKGROUND-A minimum male hemoglobin (Hb) of 13.0 g/dL will become an FDA requirement in May 2016. In addition, extending whole blood (WB) interdonation intervals (IDIs) beyond 8 weeks has been considered in order to reduce iron depletion in repeat blood donors. This study estimates the impact these changes might have on blood availability and donor iron status. STUDY DESIGN AND METHODS-Six blood centers participating in REDS-II collected information on all donation visits from 2006-09.
more » ... ons were developed from these data using a multi-stage approach that first sought to adequately reproduce the patterns of donor return, Hb and ferritin levels, and outcomes of a donor's visit (successful single or double RBC donation, deferral for low Hb) observed in REDS-II datasets. Modified simulations were used to predict the potential impact on the blood supply and donor iron status under different Hb cutoff and IDI qualification criteria. RESULTS-More than 10% of WB donations might require replacement under many simulated scenarios. Longer IDIs would reduce the proportion of donors with iron depletion, but 80% of these donors may remain iron-depleted if minimal IDIs increased to 12 or 16 weeks. CONCLUSION-Higher Hb cutoffs and longer IDIs are predicted to have a potentially large impact on collections but only a modest impact on donor iron depletion. Efforts to address iron depletion should be targeted to at-risk donors, such as iron supplementation programs for frequent donors, and policy makers should try to avoid broadly restrictive donation requirements that could substantially reduce blood availability.
doi:10.1111/trf.13663 pmid:27237451 pmcid:PMC4980222 fatcat:7bxpnsyr75gipdtce4jy44egwq

The frequency and specificity of human neutrophil antigen antibodies in a blood donor population

Jerome L. Gottschall, Darrell J. Triulzi, Brian Curtis, Ram M. Kakaiya, Michael P. Busch, Philip J. Norris, Simone A. Glynn, Danielle Carrick, David J. Wright, Steve Kleinman
2010 Transfusion  
BACKGROUND-Transfusion related acute lung injury (TRALI) has been associated with both HLA and HNA antibodies. HNA antibody frequency, specificity, and demographic associations have not been well defined in the blood donor population. METHODS-A subset of 1171 donors (388 non-transfused males, 390 HLA antibody negative females with three or more pregnancies, and 393 HLA antibody positive females with three or more pregnancies) from a larger leukocyte antibody prevalence study (LAPS) was tested
more » ... r IgG and IgM HNA antibody using a granulocyte immunofluorescence flow cytometry assay. Additional testing on selected samples included monoclonal antibody immobilization of granulocyte antigen -flow cytometry and granulocyte genotyping. RESULTS-Eight samples were HNA antibody positive (prevalence 0.7% [95% CI, 0.3 -1.3%]). Three HNA antibodies (one IgG and two IgM) were found in non-transfused males (prevalence 0.8% [95% CI, 0.2 -2.2%]); all were pan-reactive or non-specific. One HLA antibody negative previously pregnant female had an IgG HNA antibody with HNA-1a specificity (prevalence 0.3% [95% CI, 0.01-1.4%]). Four HLA antibody positive previously pregnant females demonstrated HNA antibodies, three IgG and one IgM (prevalence 1% [95% CI, 0.3 -2.6%]). Two of these were HNA-1a specific, one HNA-4a specific, and one non-specific. CONCLUSION-HNA antibodies occur with low frequency in the donor population and are present in both male and female donors. Despite the implementation of TRALI reduction strategies, HNA antibodies are still present in donor blood products. Though our data do not create a case for urgent implementation of donor HNA antibody testing, future new developments for high throughput HNA antibody screening, including for HNA-3a, may warrant reconsideration.
doi:10.1111/j.1537-2995.2010.02913.x pmid:20977484 pmcid:PMC3583295 fatcat:hutksuszgzhptp3fjbz4gl26km

Supplementary document for Synchronization-Free Top-Down Illumination Photometric Stereo Imaging Using Light-Emitting Diodes and a Mobile Device - 4962329.pdf

Emma Le Francois, Johannes Herrnsdorf, Jonathan McKendry, Laurence Broadbent, Glynn Wright, Martin D. Dawson, Michael Strain
Supplement 1
doi:10.6084/m9.figshare.13298588.v1 fatcat:jekg3gq73fd2zngk33vaxlilju

Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes [article]

David J Wright, Nicola Hall, Naomi Irish, Angela L Man, Will Glynn, Arne Mould, Alejandro De Los Angeles, Emily Angiolini, David Swarbreck, Karim Gharbi, Elizabeth M Tunbridge, Wilfried Haerty
2021 bioRxiv   pre-print
ABSTRACTAlternative splicing (AS) is a key mechanism underlying cellular differentiation and a driver of complexity in mammalian neuronal tissues. However, understanding of which isoforms are differentially used or expressed and how this affects cellular differentiation remains unclear. Long read sequencing allows full-length transcript recovery and quantification, enabling transcript-level analysis of AS processes and how these change with cell state. Here, we utilise Oxford Nanopore
more » ... es sequencing to produce a custom annotation of a well-studied human neuroblastoma cell line and to characterise isoform expression and usage across differentiation. We identify many previously unannotated features, including a novel transcript of the voltage-gated calcium channel subunit gene, CACNA2D2. We show differential expression and usage of transcripts during differentiation, and identify a putative molecular regulator underlying this state change. Our work highlights the potential of long read sequencing to uncover previously unknown transcript diversity and mechanisms influencing alternative splicing.
doi:10.1101/2021.04.27.441628 fatcat:k245idy37jfprdyf34swnlbocu

Assessment of Employee Susceptibility to Phishing Attacks at US Health Care Institutions

William J. Gordon, Adam Wright, Ranjit Aiyagari, Leslie Corbo, Robert J. Glynn, Jigar Kadakia, Jack Kufahl, Christina Mazzone, James Noga, Mark Parkulo, Brad Sanford, Paul Scheib (+1 others)
2019 JAMA Network Open  
Cybersecurity is an increasingly important threat to health care delivery, and email phishing is a major attack vector against hospital employees.
doi:10.1001/jamanetworkopen.2019.0393 pmid:30848810 pmcid:PMC6484661 fatcat:soxrticsmbd3npxkwq6h5d5x6u

Prevalence of HLA antibodies in remotely transfused or alloexposed volunteer blood donors

Ram M. Kakaiya, Darrell J. Triulzi, David J. Wright, Whitney R. Steele, Steven H. Kleinman, Michael P. Busch, Philip J. Norris, Christopher D. Hillyer, Jerome L. Gottschall, Jorge A. Rios, Patricia Carey, Simone A. Glynn
2010 Transfusion  
Background-HLA antibody testing of previously transfused or pregnant donors may help reduce the risk of transfusion-related acute lung injury (TRALI). However, the prevalence of HLA antibodies in transfused donors has not been well characterized. Methods-Transfusion and pregnancy history was obtained from consenting donors. HLA Class I & II antibody testing was performed by multi-antigen bead Luminex platform. Cut off values for class I & II antibodies used normalized background ratio of 10.8
more » ... d 6.9 respectively. Linear probability models were used to evaluate potential associations between HLA alloimmunization and donor characteristics. Results-7,920 donors (2,086 males and 5,834 females) were tested. HLA antibody prevalence did not significantly differ between 895 transfused (1.7%) and 1138 non-transfused males (1.0%), [odds ratio (OR) 1.75; 95% CI 0.80, 3.82]. Prevalence in 45 transfused nulliparous females (4.4%, 95% CI 0.1%, 11.8%) was not statistically different from the 1.6% prevalence in 1732 non-transfused nulliparous females (odds ratio 2.94, 95% CI 0.68, 12.74). Transfused parous females had higher prevalence than non-transfused counterparts (p=0.004), odds ratio 1.39 (95% CI 1.07, 1.80). In a linear probability model, the estimated additive risk of transfusion-induced alloimmunization was only 0.8% (95% CI -0.2%, 1.8%), (p=0.10). Donor transfusion history showed that 58% of transfusions occurred >10 years previously. Conclusion-Transfused volunteer blood donors do not appear to have a significantly higher prevalence of HLA antibodies than their non-transfused counterparts. Thus, in an effort to reduce TRALI risk, ascertaining past history of transfusion and testing these donors for HLA antibodies is not necessary.
doi:10.1111/j.1537-2995.2009.02556.x pmid:20070615 pmcid:PMC2891258 fatcat:r4uvionn2nd2vjge32omig7vse
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