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Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Joel Jordà, Camilo Suarez, Marc Carnicer, Angela ten Pierick, Joseph J Heijnen, Walter van Gulik, Pau Ferrer, Joan Albiol, Aljoscha Wahl
2013 BMC Systems Biology  
Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris.  ...  To obtain a detailed flux distribution of central carbon metabolism, including the pentose phosphate pathway under methanol-glucose conditions, we have applied metabolomics and instationary 13 C flux analysis  ...  Acknowledgements This study was supported by the Spanish Ministry of Science and Innovation (CICYT projects CTQ2010-15131 and PhD fellowship for J.J.), the Catalan Government (contract grant 2009-SGR-281  ... 
doi:10.1186/1752-0509-7-17 pmid:23448228 pmcid:PMC3626722 fatcat:wwnoxddhvzchvg4sklg6v2ql2u

Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

Ping Liu, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang, Siliang Zhang
2016 Bioresources and Bioprocessing  
Methods: To elucidate the impacts of glutamate on the central metabolism in detail, a combined 13 C-assisted metabolomics and 13 C metabolic flux analysis was conducted based on LC-MS/MS and GC-MS data  ...  In our present work, we found that the addition of glutamate improved the recombinant β-galactosidase (β-gal) production by P. pastoris G1HL.  ...  Glc by two-tailed T test with P = 0.05 Metabolite Glc ( Jordà and co-workers (Jordà et al. 2014 ) have studied the impact of metabolic burden on trehalose cycle in Rolproducing P. pastoris by metabolomics  ... 
doi:10.1186/s40643-016-0124-6 pmid:27867835 pmcid:PMC5093185 fatcat:jctjavxtobaklfh6asuabmnv2m

Metabolic flux partitioning between the TCA cycle and glyoxylate shunt combined with a reversible methyl citrate cycle provide nutritional flexibility for Mycobacterium tuberculosis [article]

Khushboo Borah, Tom A Mendum, Nathaniel Hawkins, Jane Ward, Michael Beale, Gerald Larrouy-Maumus, Apoorva Bhatt, Harald Pichler, Martine MOULIN, Michael Haertlein, Trevor Forsyth, Stephan Noack (+3 others)
2021 bioRxiv   pre-print
Here, we employed 13C-metabolic flux analysis using stable isotope tracers (13C and 2H) and lipid fingerprinting to investigate the metabolic network of Mtb growing slowly on physiologically relevant carbon  ...  pathways and enzymes required for carbon co-metabolism as potential drug targets.  ...  To assay for Tween 80 supernatant samples were hydrolysed by boiling for 1 532 h in methanolic KOH (5% potassium hydroxide: 50% methanol).  ... 
doi:10.1101/2021.01.29.428863 fatcat:l3c564upznasji4s455wbud3tm