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Dynamic synthetic cells based on liquid‐liquid phase separation

Nicolas Martin
2019 ChemBioChem  
recent directions towards the design of higher-order structures and behaviour.  ...  Liquid-liquid phase-separation (LLPS) processes in water are increasingly recognised as representing a viable compartmentalisation strategy through which to produce dynamic synthetic cells.  ...  Jean-Christophe Baret and Jean-Paul Douliez for helpful discussions.  ... 
doi:10.1002/cbic.201900183 pmid:31039282 fatcat:khtcq4adgjfzljgbjemskmljdu


Coin, S. Bagherpour, K.W. Gellenbeck
2000 Journal of Phycology  
G. galatheanum , like many photosynthetic dinoflagellates, contains high amounts of n-3 long-chain-polyunsaturated fatty acids (LC-PUFA) such as docosahexaenoic acid (DHA, 22:6n-3) and the hemolytic toxic  ...  Fatty acids were measured in G. galathean um grown either phototrophically, or mixotrophically with Storeatula major (Cryptophyceae) as prey.  ...  Vacuoles thus apparently serve as lysosome-like structures as well as secretory vesicles.  ... 
doi:10.1046/j.1529-8817.1999.00001-44.x fatcat:ckmutizlrrdh5e2p4d5ny7mku4

Is Research on "Synthetic Cells" Moving to the Next Level?

Pasquale Stano
2018 Life  
Here we summarize some technical and theoretical aspects of synthetic cells based on gene expression and other enzymatic reactions inside liposomes, and comment on the most recent trends.  ...  In recent years this bottom-up synthetic biology area has considerably progressed, and the field is currently experiencing a rapid expansion.  ...  Contemporary SCs are more similar to heteronomous biochemical reactors (bioreactors), scaled down to the micrometer size, than to actual autonomous living cells.  ... 
doi:10.3390/life9010003 pmid:30587790 pmcid:PMC6463193 fatcat:isd3kgjm65hdbhl4hvgj25tcjq

Lipobeads [chapter]

Sergey Kazakov
2017 Liposomes  
As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics, malaria vaccine, and dermatological agent providing different controlled release profiles without leakage  ...  The reports reviewed in this chapter demonstrate that lipobeads of nanometer and micrometer sizes can be prepared in different media, retain their stimuli responsiveness under physiological conditions,  ...  Acknowledgements Financial support for this work was provided by Pace University (Dyson College of Arts and Sciences, Summer Research and Scholarly Research Funds).  ... 
doi:10.5772/intechopen.70056 fatcat:mfpbi2ehz5d73ksvzolvvivata

Emergent Chemical Behavior in Variable-Volume Protocells

Ben Shirt-Ediss, Ricard Solé, Kepa Ruiz-Mirazo
2015 Life  
Artificial protocellular compartments and lipid vesicles have been used as model systems to understand the origins and requirements for early cells, as well as to design encapsulated reactors for biotechnology  ...  In this theoretical study, we pay attention to this often overlooked aspect and show, via the use of a simple semi-spatial vesicle reactor model, that a changing solvent volume introduces interesting non-linearities  ...  Ben Shirt-Ediss performed the computational work and wrote the Reactor Models section. Ricard V. Solé and Kepa Ruiz-Mirazo oversaw the general direction of the work.  ... 
doi:10.3390/life5010181 pmid:25590570 pmcid:PMC4390847 fatcat:ribfbq2drbbldhqk2mie24jwc4

Toward the engineering of minimal living cells

Pier Luigi Luisi
2002 The Anatomical Record  
The use of giant vesicles is also illustrated.  ...  These systems, due to their large size, offer the advantage that by way of special micro-injection techniques, all sort of biochemical agents can be directly introduced in the compartment; and that the  ...  The autocatalytic self-reproduction process has been utilized in conjunction with enzymatic reaction inside the vesicles.  ... 
doi:10.1002/ar.10155 pmid:12382319 fatcat:i4apkkzu3rhhbfklm65pflphxu

Generation of phospholipid vesicle-nanotube networks and transport of molecules therein

Aldo Jesorka, Natalia Stepanyants, Haijiang Zhang, Bahanur Ortmen, Bodil Hakonen, Owe Orwar
2011 Nature Protocols  
and microinjection, to effect shape transformations of the membrane and create a series of interconnected compartments. size and geometry of the network can be chosen according to its desired function  ...  unilamellar vesicle preparation (large unilamellar vesicle fusion or electroformation).  ...  Because of their micro-and nanoscale size and flexibility, networks are highly suitable for the construction of biomimetic chemical reactor models and for the study of the transport and interaction of  ... 
doi:10.1038/nprot.2011.321 pmid:21637199 fatcat:v4j6jufa2rfkzkuil4fehlldpu

Self-Propulsion Strategies for Artificial Cell-Like Compartments

Ibon Santiago, Friedrich C. Simmel
2019 Nanomaterials  
In this context, strategies for self-propulsion are summarized, including, compartmentalization of catalytically active particles, phoretic propulsion of vesicles and emulsion droplet motion driven by  ...  To tackle this problem, recent progress has been made that leverages the tools of active matter physics in synthetic biology.  ...  Small unilamellar vesicles (SUVs) are vesicles of 20-100 nm diameter, large unilamellar vesicles (LUVs) are 100 nm-1 µm-sized vesicles, while the term giant unilamellar vesicles (GUVs) is typically used  ... 
doi:10.3390/nano9121680 pmid:31775256 pmcid:PMC6956199 fatcat:olrji5oiwvhhrf6cpvj64hpv5q

Modelling Early Transitions Toward Autonomous Protocells [article]

Benjamin John Shirt-Ediss
2016 arXiv   pre-print
modelling work oriented in the conceptual perspective developed.  ...  Whereas an evolutionary conception of life dominates prebiotic chemistry research and overflows into the protocells field, this thesis defends that the 'autonomous systems perspective' of living phenomena  ...  as nano-sized chemical reactors.  ... 
arXiv:1606.03620v1 fatcat:pzkyjtpuorgjxbcvvv75h2p55e

Lipid vesicles in pulsed electric fields: Fundamental principles of the membrane response and its biomedical applications

Dayinta L. Perrier, Lea Rems, Pouyan E. Boukany
2017 Advances in Colloid and Interface Science  
perspectives.  ...  Afterwards, we continue with a thorough overview how electroporation and electrofusion have been used as versatile methods to manipulate vesicles of all sizes in different biomedical applications.  ...  The versatile character of vesicles in terms of their size, surface functionality, and vesicle interior makes them attractive as simple cell models and ultrasmall biomimetic reactors [23] [24] [25] [26  ... 
doi:10.1016/j.cis.2017.04.016 pmid:28499600 fatcat:jvyqcofwevarplxiov5zt5kwxm

Light Responsive Polymer Membranes: A Review

Fiore Pasquale Nicoletta, Daniela Cupelli, Patrizia Formoso, Giovanni De Filpo, Valentina Colella, Annarosa Gugliuzza
2012 Membranes  
Advances and perspectives of light responsive polymer membranes in biotechnology, chemistry and biology areas are discussed.  ...  , such as light, pH, temperature, ionic strength, pressure, magnetic field, antigen, chemical composition, and so on.  ...  Other types of self-assembly are multilayered vesicles, both small and giant ones.  ... 
doi:10.3390/membranes2010134 pmid:24957966 pmcid:PMC4021883 fatcat:6guxmysmlnephpvyf3dmrqcdty

Bioanalysis of Eukaryotic Organelles

Chad P. Satori, Michelle M. Henderson, Elyse A. Krautkramer, Vratislav Kostal, Mark M. Distefano, Edgar A. Arriaga
2013 Chemical Reviews  
"centrifugal proteomic reactor).  ...  This interest has fueled recent advances in the bioanalysis of organelles reviewed here.  ...  Hsc70 NIH3T3, RALA255-10G CFM, TEM Hsc70-positive lysosomes interacted less with autophagosomes; these lysosomes appeared prevalent in CMA; the interaction was also ATP, GTP, temperature, and lysosomal  ... 
doi:10.1021/cr300354g pmid:23570618 pmcid:PMC3676536 fatcat:3xuwqf2lpvcv3kuaoaas42oxje

Enzymatically-responsive poly(ethylene glycol) hydrogels for the controlled delivery of therapeutic peptides

Van Hove Amy, Antonienko Erin, Benoit Danielle
2016 Frontiers in Bioengineering and Biotechnology  
Beltejar, and D.S.W. Benoit. Development and in vitro assessment of enzymatically-responsive poly(ethylene glycol) hydrogels for the delivery of therapeutic peptides.  ...  presented, as are suggestions for advanced modifications and troubleshooting.  ...  International) and 1 M hydroxybenzotriazole (Advanced ChemTech) in DMF as activator and activator base for all "L" peptides.  ... 
doi:10.3389/conf.fbioe.2016.01.01371 fatcat:hqoyctfwcvdwnlpg656rvjin5e

Complex molecular mixtures under cycling gradients as basis for lifes origins [article]

Bert Poolman, Jan Spitzer
2016 bioRxiv   pre-print
We consider life as a cyclic physicochemical process that makes heredity and Darwinian evolution observable through living cells.  ...  New kinds of experimentation with crowded molecular mixtures under oscillating temperature gradients, a PCR-like mechanism of lives origins, can further illuminate how living states come about.  ...  in 'supercrowded' 3-D micro-gels within the cytoplasm, as 514 hypothesized by the sol-gel model (Spitzer & Poolman, 2013 ).  ... 
doi:10.1101/050740 fatcat:kxexoq6or5agne3leya65dqqvy

Building Reconfigurable Devices Using Complex Liquid-Fluid Interfaces

Joe Forth, Paul Y. Kim, Ganhua Xie, Xubo Liu, Brett A. Helms, Thomas P. Russell
2019 Advanced Materials  
Historically, attention in this area has focused on simple emulsions and foams, in which surface-active materials such as surfactants or colloids stabilize structures against coalescence and alter the  ...  In recent decades, however, a growing body of work has begun to demonstrate the full potential of the assembly of nanomaterials at liquid-fluid interfaces to generate functionally advanced, biomimetic  ...  Acknowledgements This article is part of the Advanced Materials Hall of Fame article series, which recognizes the excellent contributions of leading researchers to the field of materials science.  ... 
doi:10.1002/adma.201806370 pmid:30828869 fatcat:o47njimwvfgxliokoiygwtokqu
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