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DNA Persistence in a Sink Drain Environment

Eric M. Winder, George T. Bonheyo, Baochuan Lin
2015 PLoS ONE  
Extracted DNA was cloned using the pGEM-T Easy Vector System (Promega, Madison, WI) following the manufacturer's suggested protocol for ligation and chemical transformation of Escherichia coli strain DH5α  ... 
doi:10.1371/journal.pone.0134798 pmid:26230525 pmcid:PMC4521776 fatcat:artwwdfv5behrgrakhuorpvuoq

A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis

Curtis Larimer, Eric Winder, Robert Jeters, Matthew Prowant, Ian Nettleship, Raymond Shane Addleman, George T. Bonheyo
2015 Analytical and Bioanalytical Chemistry  
The accumulation of bacteria in surface-attached biofilms can be detrimental to human health, dental hygiene, and many industrial processes. Natural biofilms are soft and often transparent, and they have heterogeneous biological composition and structure over micro-and macroscales. As a result, it is challenging to quantify the spatial distribution and overall intensity of biofilms. In this work, a new method was developed to enhance the visibility and quantification of bacterial biofilms.
more » ... , broad-spectrum biomolecular staining was used to enhance the visibility of the cells, nucleic acids, and proteins that make up biofilms. Then, an image analysis algorithm was developed to objectively and quantitatively measure biofilm accumulation from digital photographs and results were compared to independent measurements of cell density. This new method was used to quantify the growth intensity of Pseudomonas putida biofilms as they grew over time. This method is simple and fast, and can quantify biofilm growth over a large area with approximately the same precision as the more laborious cell counting method. Stained and processed images facilitate assessment of spatial heterogeneity of a biofilm across a surface. This new approach to biofilm analysis could be applied in studies of natural, industrial, and environmental biofilms.
doi:10.1007/s00216-015-9195-z pmid:26643074 pmcid:PMC4709385 fatcat:rb7ihsljgzcojhhndlxc3hdzay

Enhanced Capacities of Mixed Fatty Acid-Modified Sawdust Aggregators for Remediation of Crude Oil Spill

Yongsoon Shin, Eric M. Winder, Kee Sung Han, Hongkyung Lee, George T. Bonheyo
2019 ACS Omega  
The thermograms of mixed fatty acid-modified aggregators showed two main separate degradation steps, with maximum degradation temperatures around 220°C (T d 1 ) and 270°C (T d 2 ), where T d 1 was assigned  ...  The data of weight loss at 150°C, initial decomposition temperature T d i , and decomposition temperature at maximum degradation rates, T d 1 and T d 2 , for two sets of mixed fatty acid-modified aggregators  ... 
doi:10.1021/acsomega.8b02293 pmid:31459339 pmcid:PMC6648230 fatcat:42bl27goprf4fg6mqlq4t5h26m

Partitioning of bacterial communities between travertine depositional facies at Mammoth Hot Springs, Yellowstone National Park, U.S.A

Bruce W Fouke, George T Bonheyo, Beth Sanzenbacher, Jorge Frias-Lopez
2003 Canadian journal of earth sciences (Print)  
Because the forward and reverse primer sequences are located on the pGEM-T vector flanking the cloning site, only insert DNA is amplified.  ...  and gliding within substrate biofilms and microbial mats, cell suspension in the water column, and cell attachment to H 2 O molecules that rise as steam from the water-air interface (aerosolization; Bonheyo  ... 
doi:10.1139/e03-067 fatcat:6bd2lfsylvanxjepgw7mfqoca4

Lipid biomarkers and carbon-isotopes of modern travertine deposits (Yellowstone National Park, USA): Implications for biogeochemical dynamics in hot-spring systems

Chuanlun L. Zhang, Bruce W. Fouke, George T. Bonheyo, Aaron D. Peacock, David C. White, Yongsong Huang, Christopher S. Romanek
2004 Geochimica et Cosmochimica Acta  
The dramatic decrease in 20:19c/t in these facies suggests that the relative abundance of Aquificales is much lower than in the vent (Table 3, Table 4 ).  ...  dissolved CO 2 (␦ 13 C CO2-d ) was calculated from ␦ 13 C HCO3 (Х ␦ 13 C DIC ) using the equations of ␦ 13 C CO2-d ϭ (CO2-HCO3) ϫ (1000 ϩ ␦ 13 C HCO3 )/1000 ϩ ␦ 13 C HCO3 and (CO2-HCO3) ϭ 24.12 Ϫ 9866/T,  ... 
doi:10.1016/j.gca.2004.03.005 fatcat:uavnqfgr3vfttfebm7tj5arc4y

Page 871 of SMPTE Motion Imaging Journal Vol. 81, Issue 11 [page]

1972 SMPTE Motion Imaging Journal  
Bonheyo, J. D. Clifford, A. D. Danielson, J. R. Hester and R. K.  ...  T. Scott, Eastman Kodak Company Image Transform System John D. Lowry, Image Transform Improved Emulsion and Processing Technology for Motion-Picture Color Negative Film R.G. Anderson, G. L.  ... 

Page 229 of Public Health Nursing Vol. 18, Issue 4 [page]

1926 Public Health Nursing  
Tylski, Stella T. Untiedt, Marie A. Van Der Veer, Harriet Wakeman, Laura L. Walden, Laura J. Walker, Eileen T. Walker, Evelyn T. Walters, Kathryn Webb, Mrs. T. T. Webber, Mrs. Alice S.  ...  Bonheyo, Henrietta Boorum, Mabel M. Boote, Jane Boothe, Stella Boughton, Lucina M. Boyd, Helen F. Boyesen, Boletta E. Boyle, Mrs. Mary D. Boynton, Grace Bozarth, Beatrice Brackbill, Pearl E.  ... 

Page 260 of SMPTE Motion Imaging Journal Vol. 86, Issue 4 [page]

1977 SMPTE Motion Imaging Journal  
Baptista, Richard Bartow, John Bell, George L. Bonheyo, Richard Floberg, Clyde Parker ROCKY MOUNTAIN Chairman: James D. Caron, Western Cine Service, Inc., 312 S.  ...  ., Chicago, IL 60610 Managers: Charles Crum, George Halonen, Bill Harder, Henry T. Kakehashi, Ed Rinker, Toni Roth DALLAS/FT. WORTH Chairman: Melvin E.  ... 

Page 999 of SMPTE Motion Imaging Journal Vol. 83, Issue 12 [page]

1974 SMPTE Motion Imaging Journal  
A New Color Print Film With a Short Processing Sequence Presented by Hartwell T. Sweeney for the authors, John L. Baptista, George L. Bonheyo, Reid O’Connell, Richard K. Schafer, and Eric V.  ... 

Page 36 of SMPTE Motion Imaging Journal Vol. 83, Issue 3 [page]

1974 SMPTE Motion Imaging Journal  
., Box 784, Doylestown, PA 18901 (M) BONHEYO, George L., Photographic Engr., Eastman Kodak Co.  ...  Mail: 45 Shaw rd., Baie d’Urfe 850, P.Q., Canada (A) BOVA, George J., Tech. Not. Pic., Public Archives of Canada. Mail: 180A Woodridge Cres., Ottawa K2B 7S9, Canada (M) BOWDEN, George L., Operns.  ... 

Page 35 of SMPTE Motion Imaging Journal Vol. 79, Issue 7 [page]

1970 SMPTE Motion Imaging Journal  
Mail: 1804 Stoney Brook Dr., Apt. 101, Hous- ton, Texas 77042 (A) BONHEYO, GEORGE L., Photographic Engr., Eastman Kodak Co.  ...  ., Brooklyn, N.Y. 11215 (A) BLACKHALL, GEORGE G., Tech. Rep., General Aniline & Film Corp. Mail: P.O. Box 4987, St.  ... 

Tropical microalgae isolated on Reunion island (France, Indian Ocean) as sources of antifouling molecules: The BIOPAINTROP project

Laurent Dufosse, Alexis Bazire, Yves Blache, Laurent Bleriot, Christine Bressy, Jean-François Briand, Dalyal Copin, Gérald Culioli, Maxime Delbury, Fabienne Fay, Isabelle Grondin, Isabelle Linossier (+8 others)
2016 New Biotechnology  
S10-1 The impact of microbial biofouling on marine resource extraction: a case study involving uranium adsorbents George BONHEYO 194 PT S10-2 The impact of fouling control coating selection on  ...  in Singapore Wensley Louis WIDJAJA 192 PT S9-19 The use of a simple x-ray fluorescence method (XRF) for quantification of Cu, Zn and Sn (TBT) content in marine coatings Erik YTREBERG 193 T  ... 
doi:10.1016/j.nbt.2016.06.749 fatcat:6hvbagjndjf73dhdge457bfqse

Page 7 of Convention of American Instructors of the Deaf. Report of the Proceedings of the ... Meeting of the Convention of American Instructors of the Deaf Vol. , Issue [page]

Convention of American Instructors of the Deaf. Report of the Proceedings of the ... Meeting of the Convention of American Instructors of the Deaf  
Boatwright, John T., Jacksonville, Il. 30les, Owena B., Muskegon, Mich. 30les, Mrs. Virginia, Baton Rouge, La. Boley, Louise M., Romney, W. Va. soley, Mrs. Marjorie L., Romney, W.  ...  Brown, George K., Morganton, N.C. Brown, Harold F., Morganton, N.C. Brown, Irene, Indianapolis, Ind. Brown, M. E., Baton Rouge, La. Brown, Mrs. Ola Mary, Indianapolis, Ind. Brown, Mrs.  ... 

Page 22 of University of Chicago. Law School. Law School Record Vol. 44, Issue [page]

University of Chicago. Law School. Law School Record  
Bass, George C. Best, Patricia L. Bonheyo, Gerald FE Brown, Amy H. Candido, Michael D, Chambers, Chapin F. Cimino, Katherine M. Clark, Sophie E. Clark, James E. Benjamin P. Ross E.  ...  ., Peter Bowman Rutledge, and David T. Sobota. In Bowers, Levin, addition, Sally N. Abrahamson received her degree with Highest Honors.  ... 

Development of herding agents for marine oil spill response

Miao Yu
The area of the oil slick at time t is measured and calculated followed the same procedures.  ...  Bonheyo et al. (2017) reported that aggregators could be used both as sorption and herders in oil spill response.  ... 
doi:10.48336/rwb4-4e34 fatcat:qt3lo2ijuvb57bk2rvhxxy2f5m