A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
The National Center for Biotechnology Information's (NCBI) Gene database (www.ncbi.nlm.nih.gov/gene) integrates gene-specific information from multiple data sources. NCBI Reference Sequence (RefSeq) genomes for viruses, prokaryotes and eukaryotes are the primary foundation for Gene records in that they form the critical association between sequence and a tracked gene upon which additional functional and descriptive content is anchored. Additional content is integrated based on the genomicdoi:10.1093/nar/gku1055 pmid:25355515 pmcid:PMC4383897 fatcat:s23gtmpvcnanffuoi4jnnegexa
more »... on and RefSeq transcript and protein sequence data. The content of a Gene record represents the integration of curation and automated processing from Ref-Seq, collaborating model organism databases, consortia such as Gene Ontology, and other databases within NCBI. Records in Gene are assigned unique, tracked integers as identifiers. The content (citations, nomenclature, genomic location, gene products and their attributes, phenotypes, sequences, interactions, variation details, maps, expression, homologs, protein domains and external databases) is available via interactive browsing through NCBI's Entrez system, via NCBI's Entrez programming utilities (E-Utilities and Entrez Direct) and for bulk transfer by FTP.
Even though both of these traits are associated with microfibrils, there is little phenotypic correlation (-0.13 ≤ r ≤ 0.11) and little congruence, either positive or negative, among the QTL effects for ... comparative maps between Pinus taeda × P. radiata  , whereas ESTP markers were used to create comparative maps between P. taeda and P. elliottii  and between P. taeda and P. pinaster (Chagné and Brown ...doi:10.1051/forest:2002045 fatcat:xysy5mwn7zgebiffvouub5z4n4
Homozygous seedlings ( cad-n1/cad-n1 ) obtained by selfing, contain between 0 and 1% of wild-type CAD activity and display a brown-red wood phenotype. ...doi:10.1046/j.1467-7652.2003.00024.x pmid:17163902 fatcat:i2x6ygn27rbhhjmb4hhhgbze74
High-energy radiation has been utilized for decades, however, the role of low-energy electrons created during irradiation has only recently begun to be appreciated 1,2 . Low-energy electrons are the most important component of radiation damage in biological environments because they have subcellular ranges, interact destructively with chemical bonds, and are the most abundant product of ionizing particles in tissue. However, methods for generating them locally without external stimulation dodoi:10.1038/nmat4323 pmid:26076306 fatcat:3cyowmsjhbbdjf2krvwivrre34
more »... exist. To address this we synthesized one atom thick films of the radioactive isotope 125 I on gold that are stable under ambient conditions. Scanning tunnelling microscopy, supported by electronic structure simulations, allowed us to directly observe nuclear transmutation of individual 125 I atoms into 125 Te, and explain the surprising stability of the 2-D film as it underwent radioactive decay. The metal interface geometry induces a 600% amplification of low-energy electron emission (<10 eV) 3 compared to atomic 125 I. Therefore, this enhancement of biologically active low energy electrons opens a new direction for highly targeted nanoparticle therapies 4-6 . Nuclear decay is one of the most extreme processes and is central to a range of fields including energy, medicine, imaging, labelling, archaeology and sensing. Radiation in the form of alpha particles, beta particles and gamma rays have fundamentally different interactions with matter and therefore exhibit different mean-free paths (∼1 μm, 1 mm and 1 cm, respectively). These forms of primary radiation deposit their energy over the course of their trajectory by ionizing their surroundings and producing non-thermal secondary electrons. Only very recently has the ability of low-energy secondary electrons to induce chemical reactions and biological damage begun to be appreciated 1 , because they have energies below the typical ionization threshold of organic matter. For example, low-energy electrons (3-20 eV) have been shown to be effective at causing DNA cleavage 2,4,7 . This ability stems from their high cross-section for breaking chemical bonds, and as a consequence they have a very short meanfree path of ∼1-10 nm in solution 8, 9 . Furthermore, hot electrons that are not captured by surrounding molecules become thermalized as solvated electrons which are known to be chemically and biological active 9-12 . To harness these unique properties, the design of radioactive materials that increase and localize the flux of short-range lowenergy electrons to target sites is crucial for their application in targeted cancer therapies that minimize damage to healthy cells. Thus far, it has not been possible to design atomically precise radioactive materials that maximize these effects due to self-destruction arising from nuclear recoil, Coulomb explosion and self-irradiation 13-16 . We report a straightforward method for synthesizing monolayer films of radioactive 125 I atoms on gold-coated mica substrates under ambient conditions, and characterize their composition and their electron emission. Despite being synthesized from radioactive 125 I (> 99.9% purity) they are robust with respect to self-destruction, and provide well-defined, intense planar sources of secondary electrons. 125 I decays by electron capture (EC) of a core shell electron to produce a nuclear excited state of 125 Te (Figure 1a) , the majority of which eject another core
The level of within-gene LD was low, with an average pairwise r 2 of 0.30, decaying rapidly from 0.50 to 0.20 at 800 bp. No apparent LD among genes was found. ... Gill, R. J. Kuntz, J. Beal, and J. Manares provided technical assistance in the lab. We thank A. Soto and M. T. Cervera, and P. ... -Linkage disequilibrium (as estimated by r 2 ) plots for five drought-response candidate genes, including three (dhn-1, sams-2, and ppap-12) that map in the same linkage group (LG 8; see Brown et al. ...doi:10.1534/genetics.105.047126 pmid:16387885 pmcid:PMC1456261 fatcat:dauxiamsfrbgngkieohiixa2v4
The Y chromosome was originally sequenced only to about twofold coverage as a consequence of the unusually large size of the Brown Norway Y chromosome. ... The previous rat SNP map was based on cDNA from four strains (SHRSP, Brown Norway, WKY and Sprague-Dawley) and had 12,395 interstrain polymorphic sites 4 . ...doi:10.1038/ng0508-523 pmid:18443589 pmcid:PMC2505193 fatcat:k5rnfgkj6ngzpkieh27xgwkgpa
All other plots were carried out using R (r-project.org). ... Raw counts were loaded into R (http://www.R-project.org/) (R Development Core Team, 2015) and edgeR 69 was used to perform upper quantile, betweenlane normalization, and DE analysis. ...doi:10.1101/2020.12.04.411546 fatcat:lu3gyrzmx5c2tmd6s4ea3mq7ua
ClinVar (https://www.ncbi.nlm.nih.gov/clinvar/) is a freely available, public archive of human genetic variants and interpretations of their significance to disease, maintained at the National Institutes of Health. Interpretations of the clinical significance of variants are submitted by clinical testing laboratories, research laboratories, expert panels and other groups. ClinVar aggregates data by variant-disease pairs, and by variant (or set of variants). Data aggregated by variant aredoi:10.1093/nar/gkx1153 pmid:29165669 pmcid:PMC5753237 fatcat:ngbthvyavvejjdkin52xwrjmua
more »... ble on the website, in an improved set of variant call format files and as a new comprehensive XML report. ClinVar recently started accepting submissions that are focused primarily on providing phenotypic information for individuals who have had genetic testing. Submissions may come from clinical providers providing their own interpretation of the variant ('provider interpretation') or from groups such as patient registries that primarily provide phenotypic information from patients ('phenotyping only'). ClinVar continues to make improvements to its search and retrieval functions. Several new fields are now indexed for more precise searching, and filters allow the user to narrow down a large set of search results.
. , 2002 Brown et al. 2001) . markers (Figure 1 ). ...pmid:12930758 pmcid:PMC1462646 fatcat:ob3z5hqs4je3lhezos3fqcemei
Interpretations are submitted by clinical testing laboratories, research laboratories, locus-specific databases, OMIM R , GeneReviews TM , UniProt, expert panels and practice guidelines. ... Submissions are accessioned and versioned (SCV) In its initial release (2013), ClinVar was largely seeded with records based on allelic variants described in OMIM R ; variants described in GeneReviews ... Today, ClinVar staff continue to process variants from OMIM R and GeneReviews TM ; they also regularly process direct submissions from clinical testing laboratories, research groups, UniProt and locus-specific ...doi:10.1093/nar/gkv1222 pmid:26582918 pmcid:PMC4702865 fatcat:x4b6xdfj65ebtnpinz3m2mcm6u
The National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) database is a collection of annotated genomic, transcript and protein sequence records derived from data in public sequence archives and from computation, curation and collaboration (http://www.ncbi.nlm. nih.gov/refseq/). We report here on growth of the mammalian and human subsets, changes to NCBI's eukaryotic annotation pipeline and modifications affecting transcript and protein records. Recent changes todoi:10.1093/nar/gkt1114 pmid:24259432 pmcid:PMC3965018 fatcat:wid54lruqrdzndb4oghwltdrqa
more »... s eukaryotic genome annotation pipeline provide higher throughput, and the addition of RNAseq data to the pipeline results in a significant expansion of the number of transcripts and novel exons annotated on mammalian RefSeq genomes. Recent annotation changes include reporting supporting evidence for transcript records, modification of exon feature annotation and the addition of a structured report of gene and sequence attributes of biological interest. We also describe a revised protein annotation policy for alternatively spliced transcripts with more divergent predicted proteins and we summarize the current status of the RefSeqGene project.
IntroductionFalls have a multifactorial aetiology, which may limit the effectiveness of the common approach of exercise as the sole intervention strategy. Multifactorial interventions could be more effective in people at high risk of falling; however, the focus of such interventions has traditionally been quite narrow. This paper describes the design of a randomised controlled trial that will evaluate the effectiveness of an eHealth programme, which addresses cumulative effects of key fall-riskdoi:10.1136/bmjopen-2021-051085 pmid:33858875 fatcat:ipsiqrjxpnakrhomu72ggq7b5i
more »... factors across the triad of physical, affective and cognitive functions on falls in older people.Methods and analysis518 older people aged 65 years and over with high fall risk, defined as having a history of falls in the past 6 months, self-reported fear of falling or being aged 80 years or over, will be recruited via local advertisements, newsletters and presentations, and randomised to an intervention or health education control group. The intervention comprises balance exercise, cognitive-motor exercise and cognitive–behavioural therapy, with their dosage based on participant's baseline balance, executive function and mood. The primary outcome is the rate of falls in the 12 months after randomisation. Secondary outcomes at 6 and 12 months comprise programme adherence, healthcare use, physical activity, balance and mobility, cognitive function, psychological well-being, quality of life, health literacy and user experience and attitudes towards the programme. Data will be analysed following intention to treat to gauge real-world effectiveness. We will further determine complier averaged causal effects to correct for varying adherence and conduct economic analyses to gain insight into cost-effectiveness and cost–utility.Ethics and disseminationEthical approval was obtained from the University of New South Wales (UNSW) Human Research Ethics Committee in December 2017. Outcomes will be disseminated via peer-reviewed articles, conference presentations, community events and media releases.Trial registration numberACTRN12619000540112.
The projected mass profile flattens steadily towards the center with a shallow mean slope of dΣ/d r ≃ -0.55±0.1, over the observed range, r<250kpc/h, matching well an NFW profile, but with a relatively ... The resulting mass map is more circular in projection than the clumpy distribution of cluster galaxies and the light is more concentrated than the mass within r<50kpc/h. ... Integrating the mass along a column, z, where r 2 = (ξ r r s ) 2 + z 2 gives: M(ξ r ) = ρ s r 3 s (ξ r ) ξr o d 2 ξ r ∞ −∞ 1 (r/r s )(1 + r/r s ) 2 dz r s , (22) Using this mass, a bend-angle of α = 4GM ...doi:10.1086/426494 fatcat:qyqhtpcvqvhmhihypz7oz3j5am
npj Genomic Medicine
10 −3 ), compared to both MOA (r b MOA adj ¼ 0:99, s.e. = 1.6 × 10 −3 ) and MOMENT (r b MOMENT adj ¼ 0:97, s. e. = 5.7 × 10 −3 , Supplementary Fig. 6e, f, respectively) . ... Interestingly, the correlation of effect sizes is much higher between standard linear regression models and MOA (Supplementary Fig. 1 ,r b ¼ 1, s.e. = 3 × 10 −3 ) than with MOMENT (r b ¼ À0:2, s.e. = ...doi:10.1038/s41525-020-0118-3 pmid:32140259 pmcid:PMC7046630 fatcat:wciwalvrh5ax3gqsh7jdlidr7y
1.1, (r − i) ≤ 1 and i < 27. ... 1.1, (r − i) ≤ 1 and i < 27. ...doi:10.1038/nature02125 pmid:14702079 fatcat:ogyyyvqkxrewrbuugwvvjpvkbu
« Previous Showing results 1 — 15 out of 5,219 results