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The European dimension for the mouse genome mutagenesis program

2004 Nature Genetics  
The sequencing of mammalian genomes has shown that their gene catalogs are unexpectedly small, with ∼28,000 genes.  ...  They will also facilitate the establishment of the complete null-allele set by targeting those genes that prove recalcitrant to mutagenesis by gene trapping.  ... 
doi:10.1038/ng0904-925 pmid:15340424 pmcid:PMC2716028 fatcat:5epqai3abrcjzmsnjpfkkzwyme

Mouse mutagenesis-systematic studies of mammalian gene function

S. Brown
1998 Human Molecular Genetics  
For the former, gene trap embryonic stem cell libraries appear set to deliver a large number of mutations around the mouse genome.  ...  The mouse will play a pivotal role in mammalian gene function studies as we enter the post-genomics era.  ...  This work was supported by the MRC and partly by funding from SmithKline Beecham and the EC (BMH4-CT97-2715).  ... 
doi:10.1093/hmg/7.10.1627 pmid:9735384 fatcat:3a3zupajgzcpvkmjepjonzrq4e

Global analysis of gene function in mammals: Integration of physical, mutational and expression strategies

John Schimenti
1998 Electronic Journal of Biotechnology  
The function of the genes represented by these ESTs is the great roadblock that we face. How to best go about evaluating the function of genes in a large-scale manner?  ...  New technologies are being developed to enable the simultaneous analysis of the expression patterns of thousands of genes on a large scale and under different circumstances (Chee et al., 1996; DeRisi  ... 
doi:10.2225/vol1-issue1-fulltext-5 fatcat:bdosdvyk6zfxdcfis7nflrluia

A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome

J. Hansen, T. Floss, P. Van Sloun, E.-M. Fuchtbauer, F. Vauti, H.-H. Arnold, F. Schnutgen, W. Wurst, H. von Melchner, P. Ruiz
2003 Proceedings of the National Academy of Sciences of the United States of America  
A major challenge of the postgenomic era is the functional characterization of every single gene within the mammalian genome.  ...  Thus, gene trapping allows a large-scale and cost-effective production of ES cell clones with mutations distributed throughout the genome, a resource likely to accelerate genome annotation and the in vivo  ...  We conclude that gene-trap mutagenesis is an efficient approach for annotating and dissecting the function of mammalian genes.  ... 
doi:10.1073/pnas.1633296100 pmid:12904583 pmcid:PMC187885 fatcat:arzpunfcwjbmxkkdinzlno5ay4

The mammalian gene function resource: the international knockout mouse consortium

Allan Bradley, Konstantinos Anastassiadis, Abdelkader Ayadi, James F. Battey, Cindy Bell, Marie-Christine Birling, Joanna Bottomley, Steve D. Brown, Antje Bürger, Carol J. Bult, Wendy Bushell, Francis S. Collins (+67 others)
2012 Mammalian Genome  
In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action.  ...  Now, 5 years later, the IKMC members have developed highthroughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones  ...  Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s)  ... 
doi:10.1007/s00335-012-9422-2 pmid:22968824 pmcid:PMC3463800 fatcat:4sk4ho4tinajhaje3erwogg73e

Viral Vectors as Part of an Integrated Functional Genomics Program

Janson G. Christopher, Matthew J. During
2001 Genomics  
We propose that viral vectors should be considered as part of an integrated functional genomics platform in the CNS.  ...  Over the past decade, viral vectors have slowly gained mainstream acceptance in the neuroscience and genetics communities for the in vivo study of gene function [1].  ...  The most high-throughput method of gene capture and analysis is large-scale, systematic disruption of genes, which has been implemented in both mammalian and non-mammalian systems.  ... 
doi:10.1006/geno.2001.6653 pmid:11707065 fatcat:246ksh5q3nhfxde56igpo4igiu

Genome wide functional genetics in haploid cells

Ulrich Elling, Josef M. Penninger
2014 FEBS Letters  
A single copy of the genome provides the basis for genetic analyses where any recessive mutation of essential genes will show a clear phenotype due to the absence of a second gene copy.  ...  Haploid stem cells open the possibility of combining the power of a haploid genome with pluripotency of embryonic stem cells to uncover fundamental biological processes in defined cell types at a genomic  ...  Genetic screens using haploid cells The first genome wide screens and thereby the proof of principle for functional genomics in near haploid mammalian KBM-7 cells were published by Jan Carette in the laboratory  ... 
doi:10.1016/j.febslet.2014.06.032 pmid:24950427 fatcat:k4qvlafrxzc6zag7dahq52pvay

Molecular Phenotyping of Mouse Mutant Resources by RNA Expression Profiling

J. Beckers, J. Hoheisel, W. Mewes, M. Vingron, M. de Angelis
2002 Current Genomics  
in regard of the large resources of mutant models that are currently being generated by gene-targeting and mutagenesis of the mouse genome.  ...  In yeast the true potential of large-scale transcriptome analysis in identifying regulatory units and understanding gene function has already been demonstrated by evaluating expression profiles of a comprehensive  ...  Large-scale projects have been initiated at the MRC, Harwell, United Kingdom and at the GSF, Munich, Germany, to systematically mutagenise the mouse genome either by means of chemical mutagenesis or by  ... 
doi:10.2174/1389202023350453 fatcat:2ozkqcee6zaxfgjgus5v63kgv4

Page 1631 of The Journal of Neuroscience Vol. 23, Issue 5 [page]

2003 The Journal of Neuroscience  
Of the possible routes to mutagenizing the mouse genome, gene-trapping vectors and chemical mutagenesis are be- ing used on a large scale to provide a significant number of new mutants for gene function  ...  Park, North Carolina 27709 The robotic mouse is an autosomal dominant mutant that arose from a large-scale chemical mutagenesis program.  ... 

Next-generation gene targeting in the mouse for functional genomics

Yoichi Gondo, Ryutaro Fukumura, Takuya Murata, Shigeru Makino
2009 BMB Reports  
The construction of the mutant mouse resources developed by the forward and reverse genetic mutagenesis is just the beginning of the annotation of mammalian genome.  ...  The knockout mouse mutagenesis projects with trapping/conditional mutagenesis have then followed as Phase II since 2006 by the gene-driven approach or reverse genetics.  ...  Fig. 1 . 1 Direct annotation of genome function by genetics. Fig. 2 . 2 Scheme of RIKEN gene-driven mutagenesis system (RGDMS).  ... 
doi:10.5483/bmbrep.2009.42.6.315 pmid:19558788 fatcat:nal6u6sgjzgptfn4jtueekg6tm

The mouse genetics toolkit: revealing function and mechanism

Louise van der Weyden, Jacqueline K White, David J Adams, Darren W Logan
2011 Genome Biology  
Large-scale projects are providing rapid global access to a wealth of mouse genetic resources to help discover disease genes and to manipulate their function.  ...  Acknowledgements We thank the Sanger Mouse Genetics Project for generating, managing and phenotyping the mice shown in Figure 2 . Published: 24 June 2011  ...  Gene trap mutagenesis Gene trapping in mouse ES cells is an efficient method for mutagenesis of the mammalian genome.  ... 
doi:10.1186/gb-2011-12-6-224 pmid:21722353 pmcid:PMC3218837 fatcat:72rkngkxfvbwzfxi6s5zlnaljm

Evidence for the Stochastic Integration of Gene Trap Vectors into the Mouse Germline

K. Chowdhury, P. Bonaldo, M. Torres, A. Stoykova, P. Gruss
1997 Nucleic Acids Research  
A large scale insertional mutagenesis experiment was performed in embryonic stem (ES) cells by introducing two types of gene trap vectors into the genome.  ...  In order to assess the feasibility of a large scale cloning of the targeted genes from these lines, we have isolated and characterized 55 trapped exons from the corresponding ES cells.  ...  This project was generously supported by Amgen Inc., Thousand Oak, CA and by the Max Planck Society. PB and MT were supported by EC fellowships.  ... 
doi:10.1093/nar/25.8.1531 pmid:9092658 pmcid:PMC146629 fatcat:qnh5pbkwufcvxd5micsd5b4iya

Hunting with Traps: Genome-Wide Strategies for Gene Discovery and Functional Analysis

K. Durick
1999 Genome Research  
ACKNOWLEDGMENTS We wish to acknowledge the many fine contributions to the study of genome function that could not be included in this review because of space constraints. We wish to thank Drs. D.  ...  Rink for critical review of this article. K.G.X. wishes to thank Dr. F. Craig for early inspirational discussions.  ...  It is believed that the use of random "knockout" mutagenesis by gene trapping in mice will result in further enhancements for functional analysis of the genome.  ... 
doi:10.1101/gr.9.11.1019 pmid:10568742 fatcat:7qctqjho5bc23e6pfkvkkzyo54

Genome-wide mutant collections: toolboxes for functional genomics

P Coelho
2000 Current Opinion in Microbiology  
Yeast genome research in the Snyder laboratory was supported by National Institutes of Health grant CA77808.  ...  Acknowledgements PSR Coelho was supported by the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil. A Kumar is supported by a postdoctoral fellowship from the American Cancer Society.  ...  In S. cerevisiae, two different systems of transposon insertional mutagenesis have been used for large-scale analysis of the genome.  ... 
doi:10.1016/s1369-5274(00)00095-3 pmid:10851164 fatcat:y2elgykmcjea3ptprjgv47gmbu

Germline mutagenesis mediated by Sleeping Beauty transposon system in mice

Junji Takeda, Vincent W Keng, Kyoji Horie
2007 Genome Biology  
This difference in transposition efficiency was found to be largely dependent on chromosomal status of the host genomic DNA and transposon vector design.  ...  Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice.  ...  This article has been published as part of Genome Biology Volume 8, Supplement 1, 2007: Transposons in vertebrate functional genomics.  ... 
doi:10.1186/gb-2007-8-s1-s14 pmid:18047691 pmcid:PMC2106844 fatcat:5gnb7dhjh5h6flbja43hhcoee4
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