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Fully automatic evaluation of the corneal endothelium from in vivo confocal microscopy

Bettina Selig, Koenraad A Vermeer, Bernd Rieger, Toine Hillenaar, Cris L Luengo Hendriks
2015 BMC Medical Imaging  
Manual and semi-automatic analyses of images, acquired in vivo by confocal microscopy, are often used to determine the quality of corneal endothelium in the human eye.  ...  Here, we present two fully automatic methods to analyze and quantify corneal endothelium imaged by in vivo white light slit-scanning confocal microscopy.  ...  Conclusions In this paper we have introduced an algorithm that can automatically analyze the corneal endothelium, as imaged using in vivo confocal microscopy, with a reasonable accuracy.  ... 
doi:10.1186/s12880-015-0054-3 pmid:25928199 pmcid:PMC4451752 fatcat:zq5gj5v7iba2zawoczmcj7ddou

A fully automated cell segmentation and morphometric parameter system for quantifying corneal endothelial cell morphology

Shumoos Al-Fahdawi, Rami Qahwaji, Alaa S. Al-Waisy, Stanley Ipson, Maryam Ferdousi, Rayaz A. Malik, Arun Brahma
2018 Computer Methods and Programs in Biomedicine  
Conclusions: We demonstrate the effectiveness and robustness of the CEAS system, and the possibility of utilizing it in a real world clinical setting to enable rapid diagnosis and for patient follow-up  ...  densities using a separate database of 40 images from controls (n=11), obese subjects (n=16) and patients with diabetes (n=13).  ...  obtained by in vivo corneal confocal microscopy.  ... 
doi:10.1016/j.cmpb.2018.03.015 pmid:29728238 fatcat:tsm7hkhhmvawrkumkg56t4hvjm

Corneal Endothelial Cell Segmentation by Classifier-driven Merging of Oversegmented Images

Juan P. Vigueras-Guillen, Eleni-Rosalina Andrinopoulou, Angela Engel, Hans G. Lemij, Jeroen van Rooij, Koenraad A. Vermeer, Lucas J. van Vliet
2018 IEEE Transactions on Medical Imaging  
Corneal endothelium images obtained by in vivo specular microscopy provide important information to assess the health status of the cornea.  ...  We evaluated the automatic segmentation on a data set of in vivo specular microscopy images (Topcon SP-1P), obtaining 95.8% correctly merged cells and 2.0% undersegmented cells.  ...  Krijnen for acquiring the images of our dataset.  ... 
doi:10.1109/tmi.2018.2841910 pmid:29993573 fatcat:oulhbsvihnalldudjpukkghabu

Topical 1% 5-fluorouracil in ocular surface squamous neoplasia: a long-term safety study

R. Parrozzani, D. Lazzarini, E. Alemany-Rubio, F. Urban, E. Midena
2010 British Journal of Ophthalmology  
Background/aims The aim of this study was to evaluate the long-term corneal toxicity of topical chemotherapy with 1% 5-fluorouracil (5-FU) as a sole or adjuvant treatment of ocular surface squamous neoplasia  ...  Clinical confocal microscopy showed no long-term difference between the treated eye and fellow (control) eye in: endothelial cells count, pleomorphism and polymegatism, anterior stromal keratocyte density  ...  The aim of this study was to evaluate long-term corneal toxicity of topical chemotherapy with 1% 5-FU, as a sole or adjuvant treatment of OSSN, using clinical confocal microscopy (CCM).  ... 
doi:10.1136/bjo.2010.183244 pmid:20693564 fatcat:qp36e6kbf5fshgrhfx44uh43xi

Coupling of HRT II and AS-OCT to evaluate corneal endothelial cell loss and in vivo visualization of the Ahmed glaucoma valve implant

E Mendrinos, A Dosso, J Sommerhalder, T Shaarawy
2008 Eye (London. 1987)  
Aims To report corneal endothelial cell loss and in vivo visualization of the Ahmed glaucoma valve implant in eyes with refractory glaucoma.  ...  . 36 Hara et al 33 compared the clinical efficacy of confocal biomicroscopy with that of non-contact specular microscopy for the evaluation of the corneal endothelium.  ...  The aforementioned studies evaluated white light in vivo confocal microscopy. HRT II is a laser scanning confocal microscope.  ... 
doi:10.1038/eye.2008.321 pmid:18949003 fatcat:vrwynvdugfbpjgyuetliuilctu

Confocal Microscopy of Corneal Wound Healing After Deep Lamellar Keratoplasty in Rabbits

Almamoun Abdelkader
2010 Archives of Ophthalmology  
A full-thickness graft devoid of endothelium and Descemet membrane was sutured in place. Confocal examination of lamellar interface and wound edge was performed throughout 6 months.  ...  Results: Four days postoperatively, confocal microscopy revealed numerous highly reflective keratocytes at and adjacent to the interface in all eyes, fewer in the deep than the near group.  ...  A series of confocal images was recorded as the focal plane was advanced manually or automatically from the epithelium posterior to the endothelium.  ... 
doi:10.1001/archophthalmol.2009.357 pmid:20065221 fatcat:n7a7nro7nzcljofbytpe7ba5ra

Changes in corneal parameters at confocal microscopy in treated glaucoma patients

Stefano Ranno, Fogagnolo, Rossetti, Orzalesi, Paolo Nucci
2011 Clinical Ophthalmology  
The purpose of this study was to evaluate corneal parameters in treated glaucoma patients, nontreated glaucoma patients, and normal subjects using confocal microscopy.  ...  Methods: Forty patients with primary open-angle glaucoma and 22 untreated controls underwent confocal microscopy of the cornea using the Heidelberg retinal tomograph cornea module.  ...  in vivo laser confocal microscopy of cornea The Heidelberg retina tomograph (HRT) was initially designed to evaluate the optic nerve head in glaucoma.  ... 
doi:10.2147/opth.s22874 pmid:21845031 pmcid:PMC3151567 fatcat:qg56vdgiqvfnxnzm6gj6augrhm

Examination of corneal deposits in nephropathic cystinosis using in vivo confocal microscopy and anterior segment optical coherence tomography: an age-dependent cross sectional study

Anita Csorba, Erika Maka, Otto Alexander Maneschg, Attila Szabó, Nóra Szentmáry, Mária Csidey, Miklós Resch, László Imre, Krisztina Knézy, Zoltán Zsolt Nagy
2020 BMC Ophthalmology  
Crystals appeared as randomly oriented hyperreflective, elongated structures on in vivo confocal microscopy images in all corneal layers except the endothelium.  ...  In vivo confocal microscopy and anterior segment optical coherence tomography confirmed an age-related pattern of crystal deposition.  ...  EM recruited the patients, participated in study design. ASz and MCs helped in recruiting patients. NSz, OAM, MR, IL, KK: helped in formatting, language.  ... 
doi:10.1186/s12886-020-01336-w pmid:32102651 pmcid:PMC7045449 fatcat:4pnl3qufhve5dctwk6eve74jky

Assessment of Corneal Thickness and Keratocyte Density in a Rabbit Model of Laser In Situ Keratomileusis Using Scanning Laser Confocal Microscopy

Michael D. Twa, Michael J. Giese
2011 American Journal of Ophthalmology  
In this research, we evaluate the use of in vivo scanning laser confocal microscopy to evaluate the accuracy and repeatability of in vivo measurements of corneal thickness and keratocyte density to determine  ...  of the confocal microscope. 6 Likewise, others have shown that measurements of cell density and corneal thickness differ from other standard measurement techniques, e.g. specular microscopy 7 or ultrasonic  ...  Twa, NIH/NEI: P30EY07551; confocal microscopy instrumentation provided by Heidelberg Engineering; M. Giese, None. Other Acknowledgments: The authors wish to thank Dr.  ... 
doi:10.1016/j.ajo.2011.05.023 pmid:21871603 pmcid:PMC3304505 fatcat:6cydd4pkufc5nhgsbx3db7sjem

In Vivo Confocal Microscopy of the Cornea: New Developments in Image Acquisition, Reconstruction, and Analysis Using the HRT-Rostock Corneal Module

W. Matthew Petroll, Danielle M. Robertson
2015 The ocular surface  
In vivo confocal microscopy has been used in a variety of corneal research and clinical applications since its development over 25 years ago.  ...  The optical sectioning ability of confocal microscopy allows high magnification images to be obtained from different depths within a thick tissue specimen, and is thus ideally suited to the study of intact  ...  Acknowledgments This study was supported in part by NIH R01 EY013322, EY024433, EY024546, NIH P30 EY020799, OneSight Research Foundation (DMR), and an unrestricted grant from Research to Prevent Blindness  ... 
doi:10.1016/j.jtos.2015.05.002 pmid:25998608 pmcid:PMC4499020 fatcat:cdia7i772bbojgtxqjrskg652a

Morphologic study of the cornea by in vivo confocal microscopy and optical coherence tomography after bifocal refractive corneal inlay implantation

Alex Malandrini, Gianluca Martone, Annalisa Canovetti, Luca Menabuoni, Angelo Balestrazzi, Chiara Fantozzi, Chiara Lenzetti, Marco Fantozzi
2014 Journal of cataract and refractive surgery  
PURPOSE: To evaluate the biocompatibility of the Flexivue Microlens intracorneal inlay based on healing of corneal wounds and analysis of corneal structural features using in vivo confocal microscopy (  ...  Figure 7 . 7 In vivo confocal microscopy (reconstruction of image) shows the edge of the intracorneal inlay with its central hole (scale Z 100 mm). Figure 8 . 8 In vivo confocal microscopy.  ...  Patients were asked to look straight ahead, and an in vivo confocal microscope equipped with a standard 40.0 D water-immersion front lens automatically captured images of the full thickness of the central  ... 
doi:10.1016/j.jcrs.2013.08.057 pmid:24680518 fatcat:l5rty7al45bo7kskesyqdgpyni

Unbiased corneal tissue analysis using Gabor-domain optical coherence microscopy and machine learning for automatic segmentation of corneal endothelial cells

Cristina Canavesi, Andrea Cogliati, Holly B. Hindman
2020 Journal of Biomedical Optics  
To improve accuracy and reduce bias in endothelial cell density (ECD) quantification by combining Gabor-domain optical coherence microscopy (GDOCM) for three-dimensional, wide field-of-view (1 mm2) corneal  ...  ECD obtained from automatic cell segmentation of GDOCM images yielded a correlation of 0.94 (p < 0.001) with the manual segmentation on the same images, and correlation of 0.91 (p < 0.001) with the corresponding  ...  We thank the Sight Society of Northeastern NY for providing the specular microscope analysis for the corneal tissues used in this study.  ... 
doi:10.1117/1.jbo.25.9.092902 pmid:32770867 pmcid:PMC7413309 fatcat:jskplrmmwbevvk2gmxoolgtdau

Imaging and Quantification of Subbasal Nerve Plexus in Healthy Volunteers and Diabetic Patients with or without Retinopathy

Andrey Zhivov, Karsten Winter, Marine Hovakimyan, Sabine Peschel, Volker Harder, Hans-Christof Schober, Guenther Kundt, Simone Baltrusch, Rudolf F. Guthoff, Oliver Stachs, Friedemann Paul
2013 PLoS ONE  
in vivo confocal laser-scanning microscopy (CLSM).  ...  Although automatic adapted image analysis simplifies the evaluation of in vivo CLSM data, image acquisition and quantitative analysis should be optimised for the everyday clinical practice.  ...  Engineering of in vivo confocal laser-scanning microscopy (CLSM) in recent years has facilitated examination of the human cornea.  ... 
doi:10.1371/journal.pone.0052157 pmid:23341892 pmcid:PMC3546080 fatcat:6iv4o2muafalplfk7cfwncq4kq

Corneal Confocal Microscopy in the Diagnosis of Small Fiber Neuropathy: Faster, Easier, and More Efficient Than Skin Biopsy?

Mariia V. Lukashenko, Natalia Y. Gavrilova, Anna V. Bregovskaya, Lidiia A. Soprun, Leonid P. Churilov, Ioannis N. Petropoulos, Rayaz A Malik, Yehuda Shoenfeld
2021 Pathophysiology  
Corneal confocal microscopy (CCM) is a rapid, non-invasive, reproducible method for quantifying small nerve fiber degeneration and regeneration, and could be an important tool for diagnosing SFN.  ...  This review considers the advantages and disadvantages of CCM and highlights the evolution of this technique from a research tool to a diagnostic test for small fiber damage, which can be a valuable contribution  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/pathophysiology29010001 pmid:35366285 pmcid:PMC8954271 fatcat:ips5y5aennahpn46fzm57nxz2a

Potential Factors of Corneal Endothelial Cells for Progression in Children with Uveitis

Xu Chen, Yi Shao, Shi-Nan Wu, Shan-Bi Zhou, Yuzhen Xu
2021 Disease Markers  
Corneal endothelial microscopy is one of the important methods for the detection of uveitis with high sensitivity.  ...  There are four abnormal forms of the corneal endothelium in children with uveitis: enlarged cell area gap, irregular cell shape, blurred intercellular space, and cell loss.  ...  We will use confocal microscopy in vivo to study pediatric uveitis in more detail.  ... 
doi:10.1155/2021/8432774 pmid:34992695 pmcid:PMC8727170 fatcat:u7rdqrhrvfe45miw67kkkbn65u
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