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A Case of a Patient Who Presented with Rapidly Progressive Dementia and Capricious RT-QuIC Results

Eunjoo Rhee, Sung Eun Chung, Hyung Ji Kim, Jae Hong Lee
2020 Dementia and Neurocognitive Disorders  
We should be mindful that even a highly specific diagnostic tool such as RT-QuIC test does not guarantee the diagnosis of sCJD and that it is safe to double check and repeat the test to allow for an alternative  ...  of prion disease with a sensitivity of 80%-95% and a specificity of 99% when applied in symptomatic CJD patients.  ... 
doi:10.12779/dnd.2020.19.1.36 pmid:32174052 pmcid:PMC7105714 fatcat:2w6adajqwjfg3dbyg2tb63kl7i

Formal Analysis of QUIC Handshake Protocol Using Symbolic Model Checking

Jingjing Zhang, Lin Yang, Xianming Gao, Gaigai Tang, Jiyong Zhang, Qiang Wang
2021 IEEE Access  
For a better understanding of the essential security properties, we have developed a formal model of the QUIC handshake protocol and perform a comprehensive formal security analysis by using two state-of-the-art  ...  As a newly proposed secure transport protocol, the purpose of QUIC is to improve the transport performance of HTTPS traffic and enable rapid deployment and evolution of transport mechanisms.  ...  In [25] , the authors develop a formal specification for wireless protocols and use this specification to generate an automatic randomization test tool.  ... 
doi:10.1109/access.2021.3052578 fatcat:wkbzuqn32rg3ve2qrhp6dwfpci

High Diagnostic Accuracy of RT-QuIC Assay in a Prospective Study of Patients with Suspected sCJD

Fiorini, Iselle, Perra, Bongianni, Capaldi, Sacchetto, Ferrari, Mombello, Vascellari, Testi, Monaco, Zanusso
2020 International Journal of Molecular Sciences  
); however, the combination of RT-QuIC testing of the CSF and OM samples resulted in 100% sensitivity and specificity, proving a significantly higher accuracy of RT-QuIC compared with the surrogate biomarkers  ...  The RT-QuIC assay on the CSF samples showed a 100% specificity and a 96% sensitivity, significantly higher compared with 14-3-3 (84% sensitivity and 46% specificity) and tau (85% sensitivity and 70% specificity  ...  We recently extended the RT-QuIC application to the olfactory mucosa samples of sCJD patients, reaching a high sensitivity and specificity, and we showed that the combination of CSF and OM testing by RT-QuIC  ... 
doi:10.3390/ijms21030880 pmid:32019068 pmcid:PMC7038328 fatcat:hxlqt7ibsjcxpbpcil6xffrrz4

Stability and Reproducibility Underscore Utility of RT-QuIC for Diagnosis of Creutzfeldt-Jakob Disease

Maria Cramm, Matthias Schmitz, André Karch, Eva Mitrova, Franziska Kuhn, Bjoern Schroeder, Alex Raeber, Daniela Varges, Yong-Sun Kim, Katsuya Satoh, Steven Collins, Inga Zerr
2015 Molecular Neurobiology  
However, to date, there has not been a formal multicentre assessment of the reproducibility, validity and stability of RT-QuIC in this context, an indispensable step for establishment as a diagnostic test  ...  The present study underlines the reproducibility and high stability of RT-QuIC across various CSF storage conditions with a remarkable sensitivity and specificity, suggesting RT-QuIC as an innovative and  ...  Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s)  ... 
doi:10.1007/s12035-015-9133-2 pmid:25823511 pmcid:PMC4789202 fatcat:f2a52qv5snge5pbkk7kndofceq

Prognosis: Closed-Box Analysis of Network Protocol Implementations [article]

Tiago Ferreira, Harrison Brewton, Loris D'Antoni, Alexandra Silva
2021 arXiv   pre-print
We use Prognosis to learn models of (parts of) three QUIC implementations -- Quiche (Cloudflare), Google QUIC, and Facebook mvfst -- and use these models to analyze the differences between the various  ...  techniques -- model checking temporal properties, computing differences between models of two implementations of the same protocol, or improving testing via model-based test generation.  ...  The authors would like to thank Hongqiang Liu for his help in preparing the final version of the paper.  ... 
arXiv:2201.02577v1 fatcat:ex5z5ngdd5d6xavgadv3rutuxy

Creutzfeldt–Jacob disease: new directions in diagnosis and therapeutics

Aseel Al-Ansari, N. P. Robertson
2017 Journal of Neurology  
and specific diagnostic test for sporadic CJD.  ...  However, many of the traditional diagnostic features are non-specific and only manifest later in the disease course.  ...  and specific diagnostic test for sporadic CJD.  ... 
doi:10.1007/s00415-017-8473-4 pmid:28353030 pmcid:PMC5413516 fatcat:dehmv6e7ajambjrkxs4agehew4

A Systematic Approach to Formal Analysis of QUIC Handshake Protocol Using Symbolic Model Checking

Jingjing Zhang, Xianming Gao, Lin Yang, Tao Feng, Dongyang Li, Qiang Wang, Ruhul Amin
2021 Security and Communication Networks  
To aim this safety requirement, we propose a formal analysis method to analyze the safety of QUIC handshake protocol by using model checker SPIN and cryptographic protocol verifier ProVerif.  ...  As a newly proposed secure transport protocol, QUIC aims to improve the transport performance of HTTPS traffic and enable rapid deployment and evolution of transport mechanisms.  ...  (C3) e QUIC protocol lacks a formal specification and hence is prone to ambiguity and underspecification.  ... 
doi:10.1155/2021/1630223 fatcat:b2c2f3rsdzbqpieipoa4i7cipe

Second-Generation RT-QuIC Assay for the Diagnosis of Creutzfeldt-Jakob Disease Patients in Brazil

Breno José Alencar Pires Barbosa, Bruno Batitucci Castrillo, Ricardo Pires Alvim, Marcelo Houat de Brito, Helio R. Gomes, Sônia M. D. Brucki, Jerusa Smid, Ricardo Nitrini, Michele C. Landemberger, Vilma R. Martins, Jerson L. Silva, Tuane C. R. G. Vieira
2020 Frontiers in Bioengineering and Biotechnology  
Our pilot results illustrate the feasibility of having CJD CSF samples tested in Brazilian centers and highlight the importance of interinstitutional collaboration to pursue a higher diagnostic accuracy  ...  Five out of seven suspected cases had positive tests; two cases showed inconclusive results.  ...  ACKNOWLEDGMENTS We would like to acknowledge all the patients and their families for their contributions to this study.  ... 
doi:10.3389/fbioe.2020.00929 pmid:32850757 pmcid:PMC7423993 fatcat:p2m7t3ukrjfchi543kjftytxju

State machine inference of QUIC [article]

Abdullah Rasool and Greg Alpár and Joeri de Ruiter
2019 arXiv   pre-print
This makes it a useful tool for testing and specification purposes, and to make various (future) implementations more easily comparable.  ...  QUIC is a recent transport protocol that provides reliable, secure and quick service on top of UDP in the internet.  ...  A protocol specification of a distributed program is important not only for implementation, but also interconnectivity and testing purposes.  ... 
arXiv:1903.04384v1 fatcat:rtp6moz6ubendheapn5ph3pjpy

PAD-Beads enrichment enhances detection of PrPSc using real-time quaking-induced conversion

Soyoun Hwang, Rohana P. Dassanayake, Eric M. Nicholson
2019 BMC Research Notes  
Coupling of RT-QuIC to PAD-Beads based cleanup allowed detection of PrPSc rapidly and without dilution of scrapie sheep brain homogenates prior to RT-QuIC.  ...  RT-QuIC has been used to detect PrPSc in a variety of biological tissues from brains to fluids.  ...  Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S.  ... 
doi:10.1186/s13104-019-4842-7 pmid:31836019 pmcid:PMC6911270 fatcat:p4rxuhcs3ncudptaxa233raa7u

Diagnostic accuracy of cerebrospinal fluid and blood biomarkers for the differential diagnosis of sporadic Creutzfeldt-Jakob disease: a (network) meta-analysis [article]

Nicole Rübsamen, Stephanie Pape, Stefan Konigorski, Antonia Zapf, Gerta Rücker, André Karch
2021 medRxiv   pre-print
Objective To conduct a systematic review of cerebrospinal fluid (CSF) and blood biomarkers as diagnostic tests for sporadic Creutzfeldt-Jakob disease (sCJD) in a specialised care setting and to compare  ...  RT-QuIC was the most specific biomarker (0.96 [0.86, 0.99]), and had the highest balanced accuracy (0.94). Heterogeneity in accuracy estimates was high between studies, especially for specificity.  ...  RT-QuIC was the most accurate test in among all levels of certainty of sCJD diagnosis.  ... 
doi:10.1101/2021.03.25.21254312 fatcat:z7skef53drb5rodpdury6l54t4

Prion Disease: A Challenging Diagnosis

Jeffrey Spindel, University of Louisville
2021 The University of Louisville Journal of Respiratory Infections  
Testing with Rt-QuIC was the most diagnostic laboratory test.  ...  Testing with Rt-QuIC should be performed in patients that are critically ill or may have diseases known to cause high false positive rates of 14-3-3 or total Tau.  ...  Sensitivity and specificity of lab testing were calculated with standard equations.  ... 
doi:10.18297/jri/vol5/iss1/29 fatcat:yexfctwyyvgftguq7xis7g6jnu

RT-QuIC Assays in Humans … and Animals

Steven Collins, Shannon Sarros
2016 Food Safety  
The enhanced specificity of the RT-QuIC for detection of PrPD in cerebrospinal fluid (CSF) has spawned international collaborations to rigorously assess and validate the assay for clinical diagnostic purposes  ...  Animal studies support the generic capacity of the RT-QuIC for PrPD detection, underpinning the utility of this assay for studying prion disease and the high likelihood of inter-convertibility of technical  ...  The Australian National Creuzfeldt-Jakob Disease Registry is funded by the Commonwealth Department of Health.  ... 
doi:10.14252/foodsafetyfscj.2016020 pmid:32231915 pmcid:PMC6989204 fatcat:ttmuvpugnje5rbjxud3vnamuiu

Real-Time Quaking-Induced Conversion Detection of PrPSc in Fecal Samples From Chronic Wasting Disease Infected White-Tailed Deer Using Bank Vole Substrate

Soyoun Hwang, Justin J Greenlee, Eric M Nicholson
2021 Frontiers in Veterinary Science  
More specifically, all fecal samples collected from 6 to 30 months post inoculation showed seeding activity under the conditions of study.  ...  In this study, we adapt real-time quacking-induced conversion (RT-QuIC) assays to detect the seeding activity of CWD prions in feces samples from clinical and preclinical white-tailed deer.  ...  ACKNOWLEDGMENTS The authors thank Semakaleng Lebepe-Mazur and Joseph Lesan for providing technical support for this project.  ... 
doi:10.3389/fvets.2021.643754 pmid:33748218 pmcid:PMC7969510 fatcat:bqxona7avnhp5nutnwwccq22xe

Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC

Soyoun Hwang, Trudy Tatum, Semakaleng Lebepe-Mazur, Eric M. Nicholson
2018 BMC Research Notes  
Real-time quaking induced conversion (RT-QuIC) can be used for the detection of PrP Sc in a variety of biological tissues from humans and animals.  ...  However, RT-QuIC requires a continuous supply of freshly purified prion protein and this necessity is not sustainable in a diagnostic laboratory setting.  ...  The funder of the work did not influence study design, data collection and analysis, decision to publish, and preparation of the manuscript.  ... 
doi:10.1186/s13104-018-3982-5 fatcat:mvo6pz6zkbgizk5ucdndgiddxe
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