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Fluorescent Aptamer Immobilization on Inverse Colloidal Crystals

Andrea Chiappini, Laura Pasquardini, Somayeh Nodehi, Cristina Armellini, Nicola Bazzanella, Lorenzo Lunelli, Stefano Pelli, Maurizio Ferrari, Silvia Pietralunga
2018 Sensors  
Photoluminescence measurements evidenced the effective immobilization of DNA-aptamer molecules labelled with Cy3 throughout the entire sample thickness.  ...  In this paper, we described a versatile two steps approach for the realization of silica inverse opals functionalized with DNA-aptamers labelled with Cy3 fluorophore.  ...  Figure 1 . 1 Sketch of the fabrication process for the realization of inverse silica opal and its functionalization with DNA-aptamer Cy3: (a) deposition of colloidal crystal film on the substrate, (b)  ... 
doi:10.3390/s18124326 fatcat:uzuxmsf7gre6npoecutrvkhjve

Nucleic Acids as Detection Tools [chapter]

Jeffrey C. F. Lam, Sergio Aguirre, Yingfu Li
2010 The Chemical Biology of Nucleic Acids  
These observations suggest that DNA aptamers on a nanoparticle surface can be used to control colloid stability.  ...  Since target binding on a cantilever was comparable to aptamer performance in solution, this suggests that immobilization of aptamers on cantilevers has minimal interference on detection.  ... 
doi:10.1002/9780470664001.ch19 fatcat:jho4owq3k5ephfldcgq63qbptq

Photonic Crystal Barcode: An Emerging Tool For Cancer Diagnosis

Chuyuan Ye, Danna Liang, Yu Ruan, Xuanru Lin, Ying Yu, Rubin Nan, Yongdong Yi, Weijian Sun
2021 Smart Materials in Medicine  
Photonic crystal barcodes, as one of the most powerful and stable coding methods among encoded carriers, have achieved significant success in mimetic multiplex detection of various cancer biomarkers at  ...  Accompanied by sustainable development of novel signal amplification approaches and multiplex detection systems, photonic crystal barcodes are bound to be intensively employed in clinical cancer diagnosis  ...  (a) Silica colloidal crystal beads (SCCBs) with human, mouse and rabbit IgGs. (ⅰ) Scheme of the multiplexed assay; (ⅱ) reflection images and (ⅲ) fluorescence microscope images after FITC-tagged goat antihuman  ... 
doi:10.1016/j.smaim.2021.06.003 fatcat:eepgcgsjajardmkwty55y5m7n4

Responsive Janus Structural Color Hydrogel Micromotors for Label-Free Multiplex Assays

Huan Wang, Lijun Cai, Dagan Zhang, Luoran Shang, Yuanjin Zhao
2021 Research  
Besides, the SCMs were functionalized with ion-responsive aptamers, through which the interaction between the ions and aptamers resulted in the shift of the intrinsic color of the SCMs.  ...  It was demonstrated that the SCMs could realize multiplex label-free detection of ions based on their optical coding capacity and responsive behaviors.  ...  Pt and Fe 3 O 4 NPs were immobilized on the GO sheets by simple adsorption, as shown in Figure S1 and Table S1 .  ... 
doi:10.34133/2021/9829068 pmid:34888526 pmcid:PMC8628110 fatcat:nyqsifvjujg6njw3yxvlha2jea

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Lin Xu, Zhaowei Zhang, Qi Zhang, Peiwu Li
2016 Toxins  
Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing.  ...  The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors.  ...  Fluorescence-labeled aptamer was also used for the multiple mycotoxin detection based on the fluorescence recovery. By utilizing an aptamer-photonic crystal encoded suspension array, Sun et al.  ... 
doi:10.3390/toxins8080239 pmid:27529281 pmcid:PMC4999855 fatcat:7e73v4zh65fmjgd5rn3fvflt3e

Advances in Biosensors, Chemosensors and Assays for the Determination of Fusarium Mycotoxins

Xialu Lin, Xiong Guo
2016 Toxins  
In this review, we outlined the related advances in biosensors, chemosensors and assays based on the classical and novel recognition elements such as antibodies, aptamers and molecularly imprinted polymers  ...  [88] , the surface of silica photonic crystal microphere was immobilized with the OTA or FB1 aptamers.  ...  ., a QCM-based piezoelectric immunosensor for detection of AFB1, OTA and FB1 was tested, in which the antibodies were immobilized on the DSP-coated gold quartz crystals [87] .  ... 
doi:10.3390/toxins8060161 pmid:27231937 pmcid:PMC4926128 fatcat:qk56e4ftdjbztnkvvxiiw5sywm

Noble metal nanoparticles in biosensors: recent studies and applications

Hedieh Malekzad, Parham Sahandi Zangabad, Hamed Mirshekari, Mahdi Karimi, Michael R. Hamblin
2017 Nanotechnology Reviews  
The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells.  ...  These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility.  ...  On the other hand, PtNP-dotted CNTs were implemented on the electrode to immobilize the aptamer, improve the electronic transmission, and provide a large surface area.  ... 
doi:10.1515/ntrev-2016-0014 pmid:29335674 pmcid:PMC5766271 fatcat:t5crw56x2fgblobn3srnd65w4a

Oligonucleotide-functionalized hydrogels as stimuli responsive materials and biosensors

Juewen Liu
2011 Soft Matter  
DNA, on the other hand, is a special block copolymer. Incorporation of DNA within a hydrogel network can have several important effects.  ...  For example, the hydrogel can be dried for storage and rehydrated prior to use and the immobilized DNAs are protected from nuclease cleavage.  ...  Gu and co-workers reported an interesting system where a uniform hydrogel film was prepared using silica colloidal crystal beads as a template. 96 After removal of the beads, the gel had an ordered inverse  ... 
doi:10.1039/c1sm05284e fatcat:3zxd74z4urd2boamoap3iz4f3a

User-friendly point-of-care detection of influenza A (H1N1) virus using light guide in three-dimensional photonic crystal

Nuree Lee, Cong Wang, Jungyul Park
2018 RSC Advances  
(3D) photonic crystal (PC).  ...  This paper proposes a user-friendly and highly sensitive detection method for influenza A (H1N1) virus using the cooperation of quantum dot (Qdot)-aptamer beacons and light guide in a three-dimensional  ...  ” detection principle using Qdot-aptamer conjugates and 3D nanoporous photonic crystal.  ... 
doi:10.1039/c8ra02596g pmid:35540156 pmcid:PMC9081623 fatcat:4fiutnkonfhbndko6avoovep6q

Quantum Dots: Method of Preparation and Biological Application

Amol Gomase, Sagar Sangale, Akshay Mundhe, Pravin Gadakh, Vikrant Nikam
2019 Journal of Drug Delivery and Therapeutics  
Quantum dots are inorganic semiconductor crystal of nanometer size which having distinctive conductive property depend on its size & shape.  ...  Also quantum dots having light emitting property depend on size & shape.  ...  [4] Smaller the crystal means larger the band gap. Crystal size inversely proportional to band gap. Figure 1 : Energy Diagram Of Quantum Dot.  ... 
doi:10.22270/jddt.v9i4-s.3333 fatcat:62of7hjxzfatledtxdu4u5yxe4

Biosensors for Pesticide Detection: New Trends

Audrey Sassolas, Beatriz Prieto-Simón, Jean-Louis Marty
2012 American Journal of Analytical Chemistry  
The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed.  ...  In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA.  ...  Piezoelectric Immunosensors Piezoelectric immunosensors are devices based on materials such as quartz crystals with Ab or Ag immobilized on their surface.  ... 
doi:10.4236/ajac.2012.33030 fatcat:iyvqudyb7zfgbaxtzthkeyrrrm

MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing

Marcus Menger, Aysu Yarman, Júlia Erdőssy, Huseyin Yildiz, Róbert Gyurcsányi, Frieder Scheller
2016 Biosensors  
On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified  ...  aptamers and ionic strength-dependent changes of conformation may be challenging in some application. of proteins.  ...  Therefore, the aptamer has to be immobilized directly at the surface. MIP sensors mostly apply "surface imprinted" layers directly on the electrode, QCM-crystal or SPR-chip.  ... 
doi:10.3390/bios6030035 pmid:27438862 pmcid:PMC5039654 fatcat:a3kd3tmrkfg2dnnxxiarfywiru

Aptamer-Based Biosensors for Antibiotic Detection: A Review

Asol Mehlhorn, Parvaneh Rahimi, Yvonne Joseph
2018 Biosensors  
Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity.  ...  The use of various aptamer assays to determine different groups of antibiotics like β-lactams, Aminoglycosides, Anthracyclines, Chloramphenicol, (Fluoro)Quinolones, Lincosamide, Tetracyclines and Sulfonamides  ...  The recognition probe was formed by immobilizing tetracycline specific aptamers on polymethacrylic acid-functionalized magnetite colloid nanocrystal clusters via condensation reaction.  ... 
doi:10.3390/bios8020054 pmid:29891818 pmcid:PMC6023021 fatcat:wahoxbbiijhjnhazjak26rjrsu

Current and Potential Developments of Cortisol Aptasensing towards Point-of-Care Diagnostics (POTC)

Azrul Zainol Abidin, Ruslinda Rahim, Mohd Md Arshad, Mohd Fatin Nabilah, Chun Voon, Thean-Hock Tang, Marimuthu Citartan
2017 Sensors  
We have taken the endeavour to review the available aptamer-based biosensor (aptasensor) for cortisol detection.  ...  Biotinylated aptamer against cortisol can be immobilized on the surface of the Sensor chip streptavidin (SA).  ...  Biotinylated aptamer against cortisol can be immobilized on the surface of the Sensor chip streptavidin (SA).  ... 
doi:10.3390/s17051180 pmid:28531146 pmcid:PMC5470925 fatcat:u5pyj2j2p5bk3j2hssqdlp3r2m

Classical and Modern Approaches Used for Viral Hepatitis Diagnosis

Mohammad Heiat, Reza Ranjbar, Seyed Moayed Alavian
2014 Hepatitis Monthly  
Implication for health policy/practice/research/medical education: A review on different approaches for diagnosis of viral hepatitis, in order to compare and achieve a better understanding for selecting  ...  In a QCM detection method designed for hepatitis virus detection, a recognition element is immobilized on a coated quartz crystal electrode (52) .  ...  In this technology an aptamer was immobilized on microtitration plates and an enzyme conjugated monoclonal antibody was added after HCV core antigen capturing.  ... 
doi:10.5812/hepatmon.17632 pmid:24829586 pmcid:PMC4006100 fatcat:qz642puzdvdnbnn75twwwxuyti
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