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Metabolic differences between symbiont subpopulations in the deep-sea tubeworm Riftia pachyptila
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<span title="2020-04-09">2020</span>
<i title="Cold Spring Harbor Laboratory">
bioRxiv
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Introduction 51
61 enzymes for both, glycogen generation and glycogen degradation (Markert et al., 2007, 62 Markert et al., 2011, Gardebrecht et al., 2012, Hinzke et al., 2019). ...
In contrast to previous molecular analyses that studied metabolic 85 capabilities of the Riftia symbiont population as a mixture of all cell sizes (e.g., Markert et al., 86 2007, Markert et al., 2011, ...
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Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis
<span title="2021-01-05">2021</span>
<i title="eLife Sciences Publications, Ltd">
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Markert et al., 2007; Markert et al., 2011; Gardebrecht et al., 2012) , precluding comparisons between putative subpopulations, we used a more sensitive approach. ...
Proteins were extracted as described in Hinzke and Markert, 2017 . ...
Sulfur metabolism While many of the energy-generating reactions of the uncultured Riftia symbiont's sulfur metabolism have been elucidated previously (Markert et al., 2011) , several details remained ...
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Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta: Supplementary Materials
[article]
<span title="2019-01-23">2019</span>
<i title="Cold Spring Harbor Laboratory">
bioRxiv
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Marine sponges represent one of the few eukaryotic groups that frequently harbor symbiotic members of the Thaumarchaeota, which are important chemoautotrophic ammonia-oxidizers in many environments. However, in most studies, direct demonstration of ammonia-oxidation by these archaea within sponges is lacking, and little is known about sponge-specific adaptations of ammonia-oxidizing archaea (AOA). Here, we characterized the thaumarchaeal symbiont of the marine sponge Ianthella basta using
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... oteogenomics, fluorescence in situ hybridization, qPCR and isotope-based functional assays. Candidatus Nitrosospongia bastadiensis is only distantly related to cultured AOA. It is an abundant symbiont that is solely responsible for nitrite formation from ammonia in I. basta that surprisingly does not harbor nitrite-oxidizing microbes. Furthermore, this AOA is equipped with an expanded set of extracellular subtilisin-like proteases, a metalloprotease unique among archaea, as well as a putative branched-chain amino acid ABC transporter. This repertoire is strongly indicative of a mixotrophic lifestyle and is (with slight variations) also found in other sponge-associated, but not in free-living AOA. We predict that this feature as well as an expanded and unique set of secreted serpins (protease inhibitors), a unique array of eukaryotic-like proteins, and a DNA-phosporothioation system, represent important adaptations of AOA to life within these ancient filter-feeding animals.
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Effect of metallo-β-lactamase production and multidrug resistance on clinical outcomes in patients with Pseudomonas aeruginosabloodstream infection: a retrospective cohort study
<span title="2013-11-01">2013</span>
<i title="Springer Nature">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/gryfylmjtnau5b3kr62ex5g6yy" style="color: black;">BMC Infectious Diseases</a>
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Blood stream infections (BSI) with Pseudomonas aeruginosa lead to poor clinical outcomes. The worldwide emergence and spread of metallo-β-lactamase (MBL) producing, often multidrug-resistant organisms may further aggravate this problem. Our study aimed to investigate the effect of MBL-producing P. aeruginosa (MBL-PA) and various other resistance phenotypes on clinical outcomes. Methods: A retrospective cohort study was conducted in three German hospitals. Medical files from 2006 until 2012 were
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... studied, and a number of 113 patients with P. aeruginosa BSI were included. The presence of VIM, IMP and NDM genes was detected using molecular techniques. Genetic relatedness was assessed through multilocus sequence typing (MLST). The effect of resistance patterns or MBL production on clinical outcomes was investigated by using multivariate Cox regression models. Results: In-hospital mortality was significantly higher in patients with MBL-PA and multidrug-resistant P. aeruginosa. However, neither BSI with MBL-PA nor BSI with various resistance phenotypes of P. aeruginosa were independently associated with mortality or length of hospital stay. In multivariate models, the SAPS II score (HR 1.046), appropriate definitive treatment (HR range 0.25-0.26), and cardiovascular disease (HR range 0.44-0.46) were independent predictors of mortality. Concomitant infections were associated with an excess length of stay (HR < 1). Conclusions: Medication with appropriate antimicrobial agents at any time during the course of infection remains the key for improving clinical outcomes in patients with P. aeruginosa BSI and should be combined with a strict implementation of routine infection control measures.
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Pediatric cardiac transplantation: Three-dimensional printing of anatomic models for surgical planning of heart transplantation in patients with univentricular heart
<span title="">2008</span>
<i title="Elsevier BV">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bs2uaypzlretnhi7ckxfiz3zzm" style="color: black;">Journal of Thoracic and Cardiovascular Surgery</a>
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Orthotopic heart transplantation is definitive therapy for infants with failing staged palliation after univentricular repair. 1 Although the surgical management of these patients may be quite straightforward, the actual cardiac and vascular anatomy is sometimes unpredictable. To overcome this problem, we developed a new technique to fabricate a custom made replica from 128-slice computed tomographic (CT) and magnetic resonance imaging (MRI) angiography to create a realistic model of the
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<a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/18954663">pmid:18954663</a>
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... c situation for preoperative assessment and intraoperative orientation in 2 patients with failed staged palliation. In such situations, solid replicas may be helpful in choosing surgical treatment strategies before heart transplantation, for intraoperative orientation, and in demonstrating the planned procedure to patients and parents with lifelike models. CLINICAL SUMMARY We studied 2 patients with failing single-ventricle repair who had undergone multiple surgical interventions and were finally listed for heart transplantation. Patient 1 was a 2year-old boy who had failing staged palliation of hypoplastic left heart syndrome. He had undergone a Norwood stage 1 operation, a Glenn superior cavopulmonary anastomosis, and a repair of the tricuspid valve. The patient had severe tricuspid regurgitation and heart failure after the second stage of the reconstruction and was therefore listed for transplantation. Patient 2 was a 14-year-old girl who had pulmonary atresia and a hypoplastic right ventricle without ventricular septal defect. She had undergone modified Blalock-Taussig shunt placement on the right side during her first month of life. A central shunt was created when she was 16 months old. The patient did well for the next 5 years. When she was 9 years old, a bidirectional Glenn anastomosis was performed. One year later, a total cavopulmonary connection was performed with an 18-mm Gore-Tex vascular graft (W. L. Gore & Associates, Inc, Flagstaff, Ariz). Five years after this operation, the patient was admitted to our institution with signs and symptoms of a severe protein-losing enteropathy after the Fontan procedure. Medical treatment remained unsuccessful, and the girl was finally listed for heart transplantation. In these cases, the volumetric data were processed with MeVisLab-Environment (MeVis Research, Bremen, Germany). The image data were obtained from a single CT or MRI scan (Digital Imaging and Communications in Medicine [DICOM] format). After the volume was cropped, the images were segmented with application of a region-growing algorithm. To obtain completely the preliminarily selected vascular structures, the threshold values were adjusted manually to match the borders of all structures. For creation of a surface model, a marching cube algorithm was applied. Before export of the surface data to the stereolithography machine (ZCorp, Burlington, Mass), the surface was smoothed to compensate for coarseness caused by the segmentation. The smoothing did not have any effect on the characteristics of the vascular structures of the model. For 3-dimensional (3D) printing (inkjet printing technology), the size information from the DICOM file was applied. As the size of the printed 3D model was calibrated with less than 1 mm, the size of the model matched pictured structures. 2 With these data linked to the rapid-prototype stereolithography equipment, we were able to fabricate a luminal replica of the anatomy in these patients with failed single-ventricle repair. In the newly created models, the anatomic structures important for heart transplantation, such as the single ventricle, the ascending aorta and aortic arch, the pulmonary arteries and Glenn anastomosis, the brachiocephalic vein, the superior vena cava, the inferior vena cava, and the extracardiac Fontan tunnel (in patient 2) could be reconstructed (Figures 1 and 2) . On the basis of the added anatomic understanding, the procedures for the fourth operation (patient 1) and the sixth operation (patient 2) were determined. In addition, it was possible to sterilize the model and take it into the operating room for better intraoperative orientation. DISCUSSION We previously reported the use of stereolithographic models in the correction of congenital defects with complex vascular anatomy or reoperation, cases in which surgery is often complicated by excessive scar tissue formation. 3 In patients with failing staged palliation after Norwood stage I operation, a Glenn superior cavopulmonary anastomosis, or From the
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Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta
<span title="2019-07-04">2019</span>
<i title="Wiley">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/6qawiqshrnhhhmuhwhqiacqzmy" style="color: black;">Environmental Microbiology</a>
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Marine sponges represent one of the few eukaryotic groups that frequently harbour symbiotic members of the Thaumarchaeota, which are important chemoautotrophic ammonia-oxidizers in many environments. However, in most studies, direct demonstration of ammonia-oxidation by these archaea within sponges is lacking, and little is known about sponge-specific adaptations of ammonia-oxidizing archaea (AOA). Here, we characterized the thaumarchaeal symbiont of the marine sponge Ianthella basta using
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... roteogenomics, fluorescence in situ hybridization, qPCR and isotope-based functional assays. 'Candidatus Nitrosospongia ianthellae' is only distantly related to cultured AOA. It is an abundant symbiont that is solely responsible for nitrite formation from ammonia in I. basta that surprisingly does not harbour nitrite-oxidizing microbes. Furthermore, this AOA is equipped with an expanded set of extracellular subtilisin-like proteases, a metalloprotease unique among archaea, as well as a putative branched-chain amino acid ABC transporter. This repertoire is strongly indicative of a mixotrophic lifestyle and is (with slight variations) also found in other sponge-associated, but not in free-living AOA. We predict that this feature as well as an expanded and unique set of secreted serpins (protease inhibitors), a unique array of eukaryotic-like proteins, and a DNA-phosporothioation system, represent important adaptations of AOA to life within these ancient filter-feeding animals.
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A novel in situ multiplex immunofluorescence panel for the assessment of tumor immunopathology and response to virotherapy in pediatric glioblastoma reveals a role for checkpoint protein inhibition
<span title="2019-10-21">2019</span>
<i title="Informa UK Limited">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/mvghqzcikfdhlpmzpppdyowjue" style="color: black;">Oncoimmunology</a>
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Markert and Gillespie are founders of and own stock and stock options (<8% interest) in Aettis, Inc., a biotech company that has licensed M032 HSV from The Board of Trustees of the University of Alabama ...
Drs Markert and Gillespie were also founders of and owned stock and stock options (<8%) in Catherex Inc., a biotechnology company that had licensed additional intellectual property related to oHSV. ...
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Editorial
<span title="">2019</span>
<i title="Gesellschaft fur Musiktheorie (GMTH)">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/2r2d4kyebbbejeoi257v7wbhiy" style="color: black;">Zeitschrift der Gesellschaft für Musiktheorie [Journal of the German-Speaking Society of Music Theory]</a>
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Mit Die »Anleitung zur musikalischen Setzkunst« von Gottfried Heinrich Stölzel (1690-1749) (Neumünster 2018) hat Florian Vogt eine zentrale Quelle des früheren 18. ...
Untersuchungen aus historischer und pädagogischer Perspektive (Würzburg 2018) entwickelt Malte Markert, welche neuen Perspektiven sich aus dieser Richtung für das musikalische Verstehen ergeben können. ...
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Editorial Board
<span title="">2021</span>
<i title="Institute of Electrical and Electronics Engineers (IEEE)">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/erxfd52o45errblyxi5hcekfku" style="color: black;">IEEE transactions on microwave theory and techniques</a>
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, Danilo
Manzaneque, Tomas
Mao, Junfa
Mao, Shau-Gang
Maradei, Francescaromana
Mariani, Valter
Marindra, Adi Mahmud Jaya
Marini, Stephan
Marinkovic, Zlatica
Marinov, Ognian
Mariotti, Chiara
Markert ...
Maslovski, Stanislav
Masotti, Diego
Masoumi Farahabadi,
Payam
Massa, Andrea
Mateu, Jordi
Mathis, Wolfgang
Matsushima, Akira
Mattei, Jean-Luc
Maune, Holger
McCabe, Steven
Monsef, Florian
Monti ...
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Inkludering og tilpasset opplæring for de utvalgte?
<span title="2011-05-06">2011</span>
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<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/birdqx4bcrb2pex7icmkgdj2sa" style="color: black;">Norsk pedagogisk tidsskrift</a>
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Dette er også tema for artikkelen av Florian Kiuppis: «Mer enn én vei til framtiden: Om ulike tolkninger av inkluderende opplaering». ...
Et interessant moment i denne sammenhengen er den betydelige økningen av antall norske elever som mottar spesialundervisning, der det blant annet er en markert vekst i bruk av segregerte undervisingsformer ...
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EINLEITUNG
[chapter]
<span title="1965-12-31">1965</span>
<i title="De Gruyter">
Aus den Anfängen der Sozialistischen Dramatik II
</i>
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Er ist keine zentrale Führergestalt wie Sickingen oder Florian Geyer, solche Persönlichkeiten gab es im Ruhrbergarbeiterstreik auch nicht, er wurde entfacht von der Masse der klassenbewußten Arbeiter und ...
Andere wiederum erinnern ein wenig an die Moritatengeschichten wandernder Schausteller und Bänkelsänger, so die Szene mit der Ermordung des Kommerzienrats durch den alten Markert oder die andere, in der ...
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Werkstattkonzert: Bach / StudioChor der HfMDK
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<span title="2022-01-25">2022</span>
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Viola da Gamba ↪ Ena Markert, 2. ...
Florian Lohmann. BWV 106, "Gottes Zeit ist die allerbeste Zeit" (Actus tragicus ) 1. Sonatina 2a. Coro Gottes Zeit ist die allerbeste Zeit. ...
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Modelled Development. Practices of Human Embryology at Göttingen University in the Second Half of the Twentieth Century
Modellierte Individualentwicklung. Humanembryologische Praktiken an der Universität Göttingen in der zweiten Hälfte des 20. Jahrhunderts
<span title="2020-10-06">2020</span>
<i title="Springer-Verlag">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/lshw4ec3g5elhnvh4jp4qzx6ca" style="color: black;">NTM. International Journal of History and Ethics of Natural Sciences, Technology and Medicine</a>
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Modellierte Individualentwicklung. Humanembryologische Praktiken an der Universität Göttingen in der zweiten Hälfte des 20. Jahrhunderts
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Michael Markert Abb. 1 Blick in den Sammlungsraum im Jahre 2018. ...
(Foto: Michael Markert, Göttingen) nen Blechschmidts und die Dissertationen von dessen Doktorand*innen enthielt (Abb. ). ...
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EINLEITUNG
[chapter]
<span title="1965-12-31">1965</span>
<i title="De Gruyter">
Aus den Anfängen der Sozialistischen Dramatik II
</i>
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Markert wird noch innerhalb des Stückes als der Ar-Eine Verwandtschaft mit Schillers "Kabale und Liebe" die schokierende Sensation ging. ...
Er ist keine zentrale Führergestalt wie Sickingen oder Florian Geyer, solche Persönlichkeiten gab es im Ruhrbergarbeiterstreik auch nicht, er wurde entfacht von der Masse der klassenbewußten Arbeiter und ...
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Reviewer Acknowledgements
<span title="2013-06-05">2013</span>
<i title="Springer Nature">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/hheklop5kbcmhawhlxc5lzh2sa" style="color: black;">BMC Cancer</a>
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Markert Germany Nicholas Marko United Kingdom Athina Markou Greece Iván Márquez-Rodas Spain Guido Martignoni Italy Finian Martin Ireland Juan Martinez Spain Ramon Martinez Germany Victor Martinez Canada ...
Japan
Alastair Kyle
Canada
Maria Kyrgiou
United Kingdom
Anastasios Kyriazoglou
Greece
Caterina La Porta
Italy
Carlo La Vecchia
Italy
Manuela Lacerda
Portugal
Herwig Lackner
Austria
Florian ...
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