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Characterization of a Mouse-Adapted Staphylococcus aureus Strain
2013
PLoS ONE
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Cells were harvested and washed twice in ice-cold sterile 0.5 M sucrose with 0.5 and 0.25 times the original culture volume. ...
Electropo-ration was performed in 0.1 cm cuvettes at 100 V, 2500 V and 25 uF using a Biorad GenePulser XcellTM. 0.5 M sucrose in BHI medium was added directly into the cuvette immediately afterwards. ...
doi:10.1371/journal.pone.0071142
pmid:24023720
pmcid:PMC3759423
fatcat:eoumgttfxbbl7bw2koel7hu6wa
The World Avoided by the Montreal Protocol
2008
Geophysical Research Letters
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in global mean temperature of about 0.2 K/decade [Intergovernmental Panel on Climate Change (IPCC), 2007], while a three-fold increase in CFCs might give an additional radiative forcing of about 0.6 W/m ...
These global forcings could be large: perhaps +0.5 W/m 2 due to CO 2 increases by 2020-2030, assuming scenario A1b [IPCC, 2007] , and a similar forcing from the increase in the CFCs [Velders et al., ...
doi:10.1029/2008gl034590
fatcat:4oz64osn3nanpmfnxjnuxcif6u
Revisiting Pan Evaporation Trends in Australia a Decade on
2018
Geophysical Research Letters
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Data and Methods Data were obtained from the Bureau of Meteorology (BoM) for the 41 stations considered in RRFH2007, including daily accumulations of E pan (mm/day), incoming solar R s (MJ/m 2 /day), sunshine ...
As in Johnson and Sharma (2010) , we used sunshine hours to estimate incoming solar radiation when solar exposure data were unavailable. ...
doi:10.1029/2018gl079332
fatcat:dk5npzhyxzfwhgmo6lm6jjkqre
The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing
2020
Biomedicines
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M.W.F., A.W.S. and F.M.W. receive funding from the Department of Health, Perth Children's Hospital Foundation, the Fiona Wood Foundation, and National Health and Medical Research Council (NHMRC) grants ...
Subsequently, keratinocytes produce oncostatin M (another member of the IL-6 cytokine family), which acts in a paracrine manner to stimulate profibrotic STAT3 signaling in dermal fibroblasts [40, 41] ...
doi:10.3390/biomedicines8050101
pmid:32365896
fatcat:jecp7sz365hd7ajbtcs6kmxiay
Structural Consequences of Nucleophosmin Mutations in Acute Myeloid Leukemia
2008
Journal of Biological Chemistry
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Substituted residues are colored red and those forming the NES motif are underlined. 1 M-1.cm-1-
FIGURE 3. Biophysical analysis of nucleophosmin variants. ...
Differential scanning calorimetry was used to corroborate this result and agreed within experimental error to a T m of 62°C and ⌬H unfolding of 40 kcal⅐mol Ϫ1 . ...
doi:10.1074/jbc.m801706200
pmid:18511415
pmcid:PMC2516977
fatcat:kcvco7za3jhvzfnpt7dlun3eiu
Functional response of wolves to human development across boreal North America
2019
Ecology and Evolution
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ACK N OWLED G M ENTS We thank all our collaborators who provided wolf telemetry data for this study (Table S1 ). ...
., 2006) or select for it (Bowman, Ray, Magoun, Johnson, & Dawson, 2010; Lesmerises, Dussault, & St-Laurent, 2012; Paquet et al., 2010; Whittington, St. ...
doi:10.1002/ece3.5600
pmid:31624583
pmcid:PMC6787805
fatcat:6ampegdp6baavh2hokm5evd4ty
Predictors, treatment, and outcomes of non-Pseudomonas Gram-negative peritonitis
2010
Kidney International
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2 )
25.7±5.3
25.9±6.5
0.4
eGFR at dialysis start
(ml/min per 1.73 m 2 )
7.0±5.5
7.1±4.3
0.5
Late referral
156 (24%)
955 (24%)
0.8
ESRF cause
0.3
Chronic
glomerulonephritis
192 (29% ...
Caucasian
476 (73%)
3105 (77%)
Aboriginal/Torres
Strait Islander
58 (9%)
303 (8%)
Maori/Pacific
Islander
17 (3%)
90 (2%)
Asian
80 (12%)
361 (9%)
Other
24 (4%)
161 (4%)
BMI (kg/m ...
doi:10.1038/ki.2010.149
pmid:20505659
fatcat:2y7iyuqwbnhobbuae54klazece
Encapsulating peritoneal sclerosis: incidence, predictors, and outcomes
2010
Kidney International
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Kidney International (2010) 77, 904-912 o r i g i n a l a r t i c l e DW Johnson et al.: Encapsulating peritoneal sclerosis ...
2 )
25.8 ± 6.3
26.2 ± 5.5
0.7
eGFR at dialysis start (ml/min per 1.73 m 2 )
5.3 ± 1.4 (available n=18)
7.4 ± 6.8 (available n=6077)
0.2
Late referral
11 (33%)
1765 (23%)
0.2
ESRF cause
0.4 ...
doi:10.1038/ki.2010.16
pmid:20375981
fatcat:3plz2svaejewjdthubizig6yvm
Distinctions between Hydrophobic Helices in Globular Proteins and Transmembrane Segments as Factors in Protein Sorting
2008
Journal of Biological Chemistry
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Donald M. Engelman, Yale University (24) . ...
Emission spectra were recorded at room temperature from 305 to 405 nm using a peptide concentration of 5 M. ...
Fiona Cunningham, Arianna Rath, Rachel M. Johnson and Charles M. ...
doi:10.1074/jbc.m809017200
pmid:19095650
fatcat:waw3qjqx2jehfehliiuxj3jfke
Predictors and outcomes of fungal peritonitis in peritoneal dialysis patients
2009
Kidney International
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DISCLOSURE Professor David Johnson is a consultant for Baxter Healthcare Pty Ltd and has previously received research funds from this company. ...
2 )
26.0 ± 5.0
26.0 ± 6.1
1.0
eGFR at dialysis start (ml/min per 1.73 m 2 )
6.7 ± 6.4
7.1 ± 4.4
0.4
Late referral
43 (27%)
1068 (24%)
0.6
ESRF cause
0.4
Chronic glomerulonephritis
44 (28% ...
doi:10.1038/ki.2009.202
pmid:19516241
fatcat:svaoeo3jtjctvk7r3qq7eu2ysq
Glutamate, N-acetyl aspartate and psychotic symptoms in chronic ketamine users
2013
Psychopharmacology
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Mental State -CAARMS; * p < 0.05, ** p < 0.01, *** p < 0.005) Ketamine Users
(n=15)
Controls
(n=13)
Statistical Significance
analysis
Age mean(SD)
25.7(2.1)
24.5(2.6)
t = 1.3; p = 0.2
Sex (M/ ...
doi:10.1007/s00213-013-3354-8
pmid:24264567
fatcat:s4r6ce2pwfgc7hqepxij4byqdm
Providence virus: a new member of the tetraviridae that infects cultured insect cells
2003
Virology
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in 0.5% tannic acid in 0.1 M cacodylate buffer and a further three rinses with 0.1 M cacodylate buffer. ...
In other tetraviruses, immature provirions undergo cleavage of the precursor protein into the large and small capsid proteins that comprise mature virions (Agrawal and Johnson, 1995; Johnson and Reddy ...
doi:10.1016/s0042-6822(02)00052-1
pmid:12642108
fatcat:p6bcihyycnavxgvavvdhqks2fy
Equine seroprevalence of West Nile virus antibodies in the UK in 2019
2020
Parasites & Vectors
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WNV seroprevalence was determined using two enzyme-linked immunosorbent assays (ELISAs) to detect total flavivirus antibodies and WNV-specific immunoglobulin M (IgM) antibodies. ...
Here we use a combination of total antibody and immunoglobulin M (IgM; the first antibody to be produced in vertebrates in response to antigen exposure)-specific enzyme-linked immunosorbent assays (ELISAs ...
Abbreviations ELISA: Enzyme-linked immunosorbent assay; IgM: Immunoglobulin M; TBEV: Tick-borne encephalitis virus; WNV: West Nile virus. ...
doi:10.1186/s13071-020-04481-9
pmid:33243297
fatcat:2j4nrfjnpff7pafuq4dlf2zo7a
Consolidating emerging evidence surrounding HIVST and HIVSS: a rapid systematic mapping protocol
2017
Systematic Reviews
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HIV self-testing (HIVST) is becoming popular with policy makers and commissioners globally, with a key aim of expanding access through reducing barriers to testing for individuals at risk of HIV infection. HIV self-sampling (HIVSS) was available previously to self-testing but was confined mainly to the USA and the UK. It remains to be seen whether the momentum behind HIVST will also energise efforts to expand HIVSS. Recent years have seen a rapid growth in the type of evidence related to these
doi:10.1186/s13643-017-0452-4
pmid:28381283
pmcid:PMC5382504
fatcat:ufabwqrygvcpnammi4f2zuhmwe
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... nterventions as well as several systematic reviews. The vast majority of this evidence relates to acceptability as well as values and preferences, although new types of evidence are emerging. This systematic map aims to consolidate all emerging evidence related to HIVST and HIVSS to respond to this rapidly changing area. Methods: We will systematically search databases and the abstracts of five conferences from 2006 to the present date, with monthly-automated database searches. Searches will combine key terms relating to HIV (e.g. HIV, AIDS, human immune-deficiency syndrome) with terms related to self-testing (e.g. home-test, self-test, mail-test, home dried blood spot test). Abstracts will be reviewed against inclusion criteria in duplicate. Data will be manually extracted through a standard form and then entered to an open access relational map (HIVST.org). When new and sufficient evidence emerges which addresses existing knowledge gaps, we will complete a review on a relevant topic. Discussion: This innovative approach will allow rapid cataloguing, documenting and dissemination of new evidence and key findings as they emerge into the public domain. Systematic review registration: This protocol has not been registered with PROSPERO as they do not register systematic maps.
Comprehensive nucleosome mapping of the human genome in cancer progression
2015
OncoTarget
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Altered chromatin structure is a hallmark of cancer, and inappropriate regulation of chromatin structure may represent the origin of transformation. Important studies have mapped human nucleosome distributions genome wide, but the role of chromatin structure in cancer progression has not been addressed. We developed a MNase-Transcription Start Site Sequence Capture method (mTSS-seq) to map the nucleosome distribution at human transcription start sites genome-wide in primary human lung and colon
doi:10.18632/oncotarget.6811
pmid:26735342
pmcid:PMC4924652
fatcat:alfx3fc26fh4raohuuahs4xgdu
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... adenocarcinoma tissue. Here, we confirm that nucleosome redistribution is an early, widespread event in lung (LAC) and colon (CRC) adenocarcinoma. These altered nucleosome architectures are consistent between LAC and CRC patient samples indicating that they may serve as important early adenocarcinoma markers. We demonstrate that the nucleosome alterations are driven by the underlying DNA sequence and potentiate transcription factor binding. We conclude that DNAdirected nucleosome redistributions are widespread early in cancer progression. We have proposed an entirely new hierarchical model for chromatin-mediated genome regulation.
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