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Characterization of a Mouse-Adapted Staphylococcus aureus Strain

Silva Holtfreter, Fiona J. Radcliff, Dorothee Grumann, Hannah Read, Sarah Johnson, Stefan Monecke, Stephen Ritchie, Fiona Clow, Christiane Goerke, Barbara M. Bröker, John D. Fraser, Siouxsie Wiles (+1 others)
2013 PLoS ONE  
Cells were harvested and washed twice in ice-cold sterile 0.5 M sucrose with 0.5 and 0.25 times the original culture volume.  ...  Electropo-ration was performed in 0.1 cm cuvettes at 100 V, 2500 V and 25 uF using a Biorad GenePulser XcellTM. 0.5 M sucrose in BHI medium was added directly into the cuvette immediately afterwards.  ... 
doi:10.1371/journal.pone.0071142 pmid:24023720 pmcid:PMC3759423 fatcat:eoumgttfxbbl7bw2koel7hu6wa

The World Avoided by the Montreal Protocol

Olaf Morgenstern, Peter Braesicke, Margaret M. Hurwitz, Fiona M. O'Connor, Andrew C. Bushell, Colin E. Johnson, John A. Pyle
2008 Geophysical Research Letters  
in global mean temperature of about 0.2 K/decade [Intergovernmental Panel on Climate Change (IPCC), 2007], while a three-fold increase in CFCs might give an additional radiative forcing of about 0.6 W/m  ...  These global forcings could be large: perhaps +0.5 W/m 2 due to CO 2 increases by 2020-2030, assuming scenario A1b [IPCC, 2007] , and a similar forcing from the increase in the CFCs [Velders et al.,  ... 
doi:10.1029/2008gl034590 fatcat:4oz64osn3nanpmfnxjnuxcif6u

Revisiting Pan Evaporation Trends in Australia a Decade on

Clare M. Stephens, Tim R. McVicar, Fiona M. Johnson, Lucy A. Marshall
2018 Geophysical Research Letters  
Data and Methods Data were obtained from the Bureau of Meteorology (BoM) for the 41 stations considered in RRFH2007, including daily accumulations of E pan (mm/day), incoming solar R s (MJ/m 2 /day), sunshine  ...  As in Johnson and Sharma (2010) , we used sunshine hours to estimate incoming solar radiation when solar exposure data were unavailable.  ... 
doi:10.1029/2018gl079332 fatcat:dk5npzhyxzfwhgmo6lm6jjkqre

The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing

Blair Z. Johnson, Andrew W. Stevenson, Cecilia M. Prêle, Mark W. Fear, Fiona M. Wood
2020 Biomedicines  
M.W.F., A.W.S. and F.M.W. receive funding from the Department of Health, Perth Children's Hospital Foundation, the Fiona Wood Foundation, and National Health and Medical Research Council (NHMRC) grants  ...  Subsequently, keratinocytes produce oncostatin M (another member of the IL-6 cytokine family), which acts in a paracrine manner to stimulate profibrotic STAT3 signaling in dermal fibroblasts [40, 41]  ... 
doi:10.3390/biomedicines8050101 pmid:32365896 fatcat:jecp7sz365hd7ajbtcs6kmxiay

Structural Consequences of Nucleophosmin Mutations in Acute Myeloid Leukemia

Charles G. Grummitt, Fiona M. Townsley, Christopher M. Johnson, Alan J. Warren, Mark Bycroft
2008 Journal of Biological Chemistry  
Substituted residues are colored red and those forming the NES motif are underlined. 1 M-1.cm-1- FIGURE 3. Biophysical analysis of nucleophosmin variants.  ...  Differential scanning calorimetry was used to corroborate this result and agreed within experimental error to a T m of 62°C and ⌬H unfolding of 40 kcal⅐mol Ϫ1 .  ... 
doi:10.1074/jbc.m801706200 pmid:18511415 pmcid:PMC2516977 fatcat:kcvco7za3jhvzfnpt7dlun3eiu

Functional response of wolves to human development across boreal North America

Tyler B. Muhly, Cheryl A. Johnson, Mark Hebblewhite, Eric W. Neilson, Daniel Fortin, John M. Fryxell, Andrew David M. Latham, Maria C. Latham, Philip D. McLoughlin, Evelyn Merrill, Paul C. Paquet, Brent R. Patterson (+3 others)
2019 Ecology and Evolution  
ACK N OWLED G M ENTS We thank all our collaborators who provided wolf telemetry data for this study (Table S1 ).  ...  ., 2006) or select for it (Bowman, Ray, Magoun, Johnson, & Dawson, 2010; Lesmerises, Dussault, & St-Laurent, 2012; Paquet et al., 2010; Whittington, St.  ... 
doi:10.1002/ece3.5600 pmid:31624583 pmcid:PMC6787805 fatcat:6ampegdp6baavh2hokm5evd4ty

Predictors, treatment, and outcomes of non-Pseudomonas Gram-negative peritonitis

Elizabeth M. Jarvis, Carmel M. Hawley, Stephen P. McDonald, Fiona G. Brown, Johan B. Rosman, Kathryn J. Wiggins, Kym M. Bannister, David W. Johnson
2010 Kidney International  
2 ) 25.7±5.3 25.9±6.5 0.4 eGFR at dialysis start (ml/min per 1.73 m 2 ) 7.0±5.5 7.1±4.3 0.5 Late referral 156 (24%) 955 (24%) 0.8 ESRF cause 0.3 Chronic glomerulonephritis 192 (29%  ...  Caucasian 476 (73%) 3105 (77%) Aboriginal/Torres Strait Islander 58 (9%) 303 (8%) Maori/Pacific Islander 17 (3%) 90 (2%) Asian 80 (12%) 361 (9%) Other 24 (4%) 161 (4%) BMI (kg/m  ... 
doi:10.1038/ki.2010.149 pmid:20505659 fatcat:2y7iyuqwbnhobbuae54klazece

Encapsulating peritoneal sclerosis: incidence, predictors, and outcomes

David W. Johnson, Yeoungjee Cho, Brian E.R. Livingston, Carmel M. Hawley, Stephen P. McDonald, Fiona G. Brown, Johan B. Rosman, Kym M. Bannister, Kathryn J. Wiggins
2010 Kidney International  
Kidney International (2010) 77, 904-912 o r i g i n a l a r t i c l e DW Johnson et al.: Encapsulating peritoneal sclerosis  ...  2 ) 25.8 ± 6.3 26.2 ± 5.5 0.7 eGFR at dialysis start (ml/min per 1.73 m 2 ) 5.3 ± 1.4 (available n=18) 7.4 ± 6.8 (available n=6077) 0.2 Late referral 11 (33%) 1765 (23%) 0.2 ESRF cause 0.4  ... 
doi:10.1038/ki.2010.16 pmid:20375981 fatcat:3plz2svaejewjdthubizig6yvm

Distinctions between Hydrophobic Helices in Globular Proteins and Transmembrane Segments as Factors in Protein Sorting

Fiona Cunningham, Arianna Rath, Rachel M. Johnson, Charles M. Deber
2008 Journal of Biological Chemistry  
Donald M. Engelman, Yale University (24) .  ...  Emission spectra were recorded at room temperature from 305 to 405 nm using a peptide concentration of 5 M.  ...  Fiona Cunningham, Arianna Rath, Rachel M. Johnson and Charles M.  ... 
doi:10.1074/jbc.m809017200 pmid:19095650 fatcat:waw3qjqx2jehfehliiuxj3jfke

Predictors and outcomes of fungal peritonitis in peritoneal dialysis patients

Rhianna Miles, Carmel M. Hawley, Stephen P. McDonald, Fiona G. Brown, Johan B. Rosman, Kathryn J. Wiggins, Kym M. Bannister, David W. Johnson
2009 Kidney International  
DISCLOSURE Professor David Johnson is a consultant for Baxter Healthcare Pty Ltd and has previously received research funds from this company.  ...  2 ) 26.0 ± 5.0 26.0 ± 6.1 1.0 eGFR at dialysis start (ml/min per 1.73 m 2 ) 6.7 ± 6.4 7.1 ± 4.4 0.4 Late referral 43 (27%) 1068 (24%) 0.6 ESRF cause 0.4 Chronic glomerulonephritis 44 (28%  ... 
doi:10.1038/ki.2009.202 pmid:19516241 fatcat:svaoeo3jtjctvk7r3qq7eu2ysq

Glutamate, N-acetyl aspartate and psychotic symptoms in chronic ketamine users

James M. Stone, Fiona Pepper, Johnson Fam, Hannah Furby, Emer Hughes, Celia Morgan, Oliver D. Howes
2013 Psychopharmacology  
Mental State -CAARMS; * p < 0.05, ** p < 0.01, *** p < 0.005) Ketamine Users (n=15) Controls (n=13) Statistical Significance analysis Age mean(SD) 25.7(2.1) 24.5(2.6) t = 1.3; p = 0.2 Sex (M/  ... 
doi:10.1007/s00213-013-3354-8 pmid:24264567 fatcat:s4r6ce2pwfgc7hqepxij4byqdm

Providence virus: a new member of the tetraviridae that infects cultured insect cells

Fiona M Pringle, Karyn N Johnson, Cynthia L Goodman, Arthur H McIntosh, L.Andrew Ball
2003 Virology  
in 0.5% tannic acid in 0.1 M cacodylate buffer and a further three rinses with 0.1 M cacodylate buffer.  ...  In other tetraviruses, immature provirions undergo cleavage of the precursor protein into the large and small capsid proteins that comprise mature virions (Agrawal and Johnson, 1995; Johnson and Reddy  ... 
doi:10.1016/s0042-6822(02)00052-1 pmid:12642108 fatcat:p6bcihyycnavxgvavvdhqks2fy

Equine seroprevalence of West Nile virus antibodies in the UK in 2019

Arran J. Folly, Elisabeth S. L. Waller, Fiona McCracken, Lorraine M. McElhinney, Helen Roberts, Nicholas Johnson
2020 Parasites & Vectors  
WNV seroprevalence was determined using two enzyme-linked immunosorbent assays (ELISAs) to detect total flavivirus antibodies and WNV-specific immunoglobulin M (IgM) antibodies.  ...  Here we use a combination of total antibody and immunoglobulin M (IgM; the first antibody to be produced in vertebrates in response to antigen exposure)-specific enzyme-linked immunosorbent assays (ELISAs  ...  Abbreviations ELISA: Enzyme-linked immunosorbent assay; IgM: Immunoglobulin M; TBEV: Tick-borne encephalitis virus; WNV: West Nile virus.  ... 
doi:10.1186/s13071-020-04481-9 pmid:33243297 fatcat:2j4nrfjnpff7pafuq4dlf2zo7a

Consolidating emerging evidence surrounding HIVST and HIVSS: a rapid systematic mapping protocol

T. Charles Witzel, Peter Weatherburn, Fiona M. Burns, Cheryl C. Johnson, Carmen Figueroa, Alison J. Rodger
2017 Systematic Reviews  
HIV self-testing (HIVST) is becoming popular with policy makers and commissioners globally, with a key aim of expanding access through reducing barriers to testing for individuals at risk of HIV infection. HIV self-sampling (HIVSS) was available previously to self-testing but was confined mainly to the USA and the UK. It remains to be seen whether the momentum behind HIVST will also energise efforts to expand HIVSS. Recent years have seen a rapid growth in the type of evidence related to these
more » ... nterventions as well as several systematic reviews. The vast majority of this evidence relates to acceptability as well as values and preferences, although new types of evidence are emerging. This systematic map aims to consolidate all emerging evidence related to HIVST and HIVSS to respond to this rapidly changing area. Methods: We will systematically search databases and the abstracts of five conferences from 2006 to the present date, with monthly-automated database searches. Searches will combine key terms relating to HIV (e.g. HIV, AIDS, human immune-deficiency syndrome) with terms related to self-testing (e.g. home-test, self-test, mail-test, home dried blood spot test). Abstracts will be reviewed against inclusion criteria in duplicate. Data will be manually extracted through a standard form and then entered to an open access relational map (HIVST.org). When new and sufficient evidence emerges which addresses existing knowledge gaps, we will complete a review on a relevant topic. Discussion: This innovative approach will allow rapid cataloguing, documenting and dissemination of new evidence and key findings as they emerge into the public domain. Systematic review registration: This protocol has not been registered with PROSPERO as they do not register systematic maps.
doi:10.1186/s13643-017-0452-4 pmid:28381283 pmcid:PMC5382504 fatcat:ufabwqrygvcpnammi4f2zuhmwe

Comprehensive nucleosome mapping of the human genome in cancer progression

Brooke R. Druliner, Daniel Vera, Ruth Johnson, Xiaoyang Ruan, Lynn M. Apone, Eileen T. Dimalanta, Fiona J. Stewart, Lisa Boardman, Jonathan H. Dennis
2015 OncoTarget  
Altered chromatin structure is a hallmark of cancer, and inappropriate regulation of chromatin structure may represent the origin of transformation. Important studies have mapped human nucleosome distributions genome wide, but the role of chromatin structure in cancer progression has not been addressed. We developed a MNase-Transcription Start Site Sequence Capture method (mTSS-seq) to map the nucleosome distribution at human transcription start sites genome-wide in primary human lung and colon
more » ... adenocarcinoma tissue. Here, we confirm that nucleosome redistribution is an early, widespread event in lung (LAC) and colon (CRC) adenocarcinoma. These altered nucleosome architectures are consistent between LAC and CRC patient samples indicating that they may serve as important early adenocarcinoma markers. We demonstrate that the nucleosome alterations are driven by the underlying DNA sequence and potentiate transcription factor binding. We conclude that DNAdirected nucleosome redistributions are widespread early in cancer progression. We have proposed an entirely new hierarchical model for chromatin-mediated genome regulation.
doi:10.18632/oncotarget.6811 pmid:26735342 pmcid:PMC4924652 fatcat:alfx3fc26fh4raohuuahs4xgdu
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