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A mRNA determinant of gRNA-directed kinetoplastid editing

A. L. Kabb
2001 Nucleic Acids Research  
The sequence adjacent to most mRNA editing sites has a high purine content which previously has been proposed to facilitate the editing reaction through base-pairing to a poly(U) tail at the 3′ end of  ...  We demonstrate here that gRNA binding alone is insufficient to create an editing site and that the mRNA sequence near an editing site is an additional determinant affecting the efficiency of the reaction  ...  The 3′ extended gRNA also directed editing of the mRNA transcript containing the wild-type purine sequence as well as the non-extended gRNA (Fig. 1C , lanes 4 and 5).  ... 
doi:10.1093/nar/29.12.2575 pmid:11410666 pmcid:PMC55732 fatcat:56wddkpswzboxihv25ayrwwt4y

RNA editing of apolipoprotein B mRNA. Sequence specificity determined by in vitro coupled transcription editing

S H Chen, X X Li, W S Liao, J H Wu, L Chan
1990 Journal of Biological Chemistry  
Among 22 different mutant apoB-100 minigene constructs containing mutations in the bases immediately flanking the edited C-6666, 20 were edited in the coupled transcription-editing reaction.  ...  Efficient RNA editing was demonstrated by using apoB mRNA segments as substrate or in a coupled transcription-editing reaction using apoB minigenes as template.  ...  edited somewhat more efficiently (-1.5fold) than wild type.  ... 
pmid:2324099 fatcat:fnzz7izzs5gfxbnkfr5gig2kru

DYW-type PPR proteins in a heterolobosean protist: Plant RNA editing factors involved in an ancient horizontal gene transfer?

Volker Knoop, Mareike Rüdinger
2010 FEBS Letters  
Several PLS proteins with such domain extensions (E, E+, DYW) were shown to be involved in plant organellar RNA editing but their evolutionary origin had remained enigmatic.  ...  A particular type of pentatricopeptide repeat (PPR) proteins with variable length of the 35 aa PPR motifs and conserved carboxyterminal extensions, named the PLS proteins, was so far exclusively identified  ...  The numerous P-type PPR proteins of Naegleria may have served as the evolutionary origin of the PLS type proteins with domain extensions.  ... 
doi:10.1016/j.febslet.2010.09.041 pmid:20888816 fatcat:xkpmy5gsvfgsfgag52e22at5wm

Page 1143 of SMPTE Motion Imaging Journal Vol. 96, Issue 11 [page]

1987 SMPTE Motion Imaging Journal  
fied file to replace the include directive in the edit decision list.  ...  , immediately following the VIRTUAL EDIT INDICATOR SUBFIELD, which may be empty. 4.1.4 Element Type Identifier Subfield. The ELE- MENT TYPE IDENTIFIER SUBFIELD indi- cates the type of ELEMENT.  ... 

Activation of Guide RNA-directed Editing of a CytochromebmRNA

Lisa M. Oppegard, Aaron L. Kabb, Gregory J. Connell
2000 Journal of Biological Chemistry  
Mutation of the sequence on the guide RNA stimulates directed editing and results in the loss of binding to at least one component within the editing extract.  ...  Although in vitro systems have been valuable in characterizing the editing mechanism, only a limited number of mRNAs are accurately edited in vitro.  ...  Templates RESULTS gRNA-directed Editing of a Cytochrome b Transcript-A primer extension assay was used to detect in vitro gRNAdirected U-insertions occurring within the first editing site of a pre-edited  ... 
doi:10.1074/jbc.m003002200 pmid:10940300 fatcat:w23dtp2aezh3xmpxefiuwnp4ie

Overexpression of APOBEC-1 Results in Mooring Sequence-dependent Promiscuous RNA Editing

Mark Sowden, Jonathan K. Hamm, Harold C. Smith
1996 Journal of Biological Chemistry  
Additional site editing was a direct consequence of overexpressing APOBEC-1.  ...  A macromolecular complex or "editosome" specifically assembles upon the mooring sequence (11) and directs editing of cytidine residues appropriately located in only the 5Ј direction (10).  ...  In this scenario, editing of the promiscuous sites could be directed from a single editosome assembled at the wild type mooring sequence (nt 6670 -6681).  ... 
doi:10.1074/jbc.271.6.3011 pmid:8621694 fatcat:yw7b73jmczgvlmiq3xzlxlt2km

Living it up with a live programming language

Sean McDirmid
2007 SIGPLAN notices  
A dynamic language improves programmer productivity through flexible typing, a focus on high-level programming, and by streamlining the edit-compile-debug cycle.  ...  A live language supports live programming that provides programmers with responsive and continuous feedback about how their edits affect program execution.  ...  When editing changes an extension rule that targets an inferred type, all symbols whose inferred types are subtypes of this type must be damaged.  ... 
doi:10.1145/1297105.1297073 fatcat:zijbucb3gbg4debgvxtsw7tima

Living it up with a live programming language

Sean McDirmid
2007 Proceedings of the 22nd annual ACM SIGPLAN conference on Object oriented programming systems and applications - OOPSLA '07  
A dynamic language improves programmer productivity through flexible typing, a focus on high-level programming, and by streamlining the edit-compile-debug cycle.  ...  A live language supports live programming that provides programmers with responsive and continuous feedback about how their edits affect program execution.  ...  When editing changes an extension rule that targets an inferred type, all symbols whose inferred types are subtypes of this type must be damaged.  ... 
doi:10.1145/1297027.1297073 dblp:conf/oopsla/McDirmid07 fatcat:h6bi6smcwvhwpesnkr7xmjmepy

Page 993 of SMPTE Motion Imaging Journal Vol. 95, Issue 9 [page]

1986 SMPTE Motion Imaging Journal  
causes the contents of the speci- fied file to replace the include directive in the edit decision list.  ...  -l Element Type Identifier Subfield. The ELE MENT TYPE IDENTIFIER SUBFIELD indi cates the type of ELEMENT.  ... 

Induction of RNA editing at heterologous sites by sequences in apolipoprotein B mRNA

D M Driscoll, S Lakhe-Reddy, L M Oleksa, D Martinez
1993 Molecular and Cellular Biology  
RNAs with mutations in this efficiency sequence were edited at 22 to 160% of wild-type levels.  ...  Editing was detected by a sensitive and linear primer extension assay. An upstream region (nt 6648 to 6661) which affected the efficiency of editing was identified.  ...  To analyze editing at nt 6597 and at the wild-type site at nt 6666, we performed primer extension using two different primers.  ... 
doi:10.1128/mcb.13.12.7288 pmid:8246950 pmcid:PMC364799 fatcat:zzcncgmxvnemtloxbooul3rhua

Sequence requirements for the editing of apolipoprotein B mRNA

R R Shah, T J Knott, J E Legros, N Navaratnam, J C Greeve, J Scott
1991 Journal of Biological Chemistry  
To establish the sequences required for editing we have used an in vitro conversion assay to monitor the editing of synthetic RNAs by rat intestinal extracts.  ...  These results suggest that editing is a highly sequence-specific process. We propose that this downstream region is a recognition and/or binding site for the editing enzyme.  ...  of wild type.  ... 
pmid:1885564 fatcat:x6gunrgiv5fnbftyx7alcixuvy

Mitochondrial RNA editing PPR proteins can tolerate protein tags at E as well as at DYW domain termini

Nadja Brehme, Anja Zehrmann, Daniil Verbitskiy, Barbara Härtel, Mizuki Takenaka
2014 Frontiers in Plant Science  
MOST E DOMAIN PPR EDITING PROTEINS ARE FULLY FUNCTIONAL WITH A C-TERMINAL GFP EXTENSION As representatives for the PPR type sitespecific mitochondrial RNA editing factors (MEF) terminating with an E domain  ...  PERSPECTIVE In plant organellar RNA editing, specific sites are addressed by PPR proteins which all contain C-terminal extension domains.  ... 
doi:10.3389/fpls.2014.00127 pmid:24772113 pmcid:PMC3982070 fatcat:drenhen4pzgrnjta2csdibbmlq

Page 112 of JEGP (Journal of English and Germanic Philology) Vol. 42, Issue 1 [page]

1943 JEGP (Journal of English and Germanic Philology)  
That he was likely to make changes after that date appears from his revisions of the poems between 1726 and 1730; his extensive revision in 1743—44 for the editions of 1744; his revision of the 1744 small  ...  octavo Type A for the 1744 Type B, producing a number of readings whose first appearance has been assigned to the 1746 Seasons ;'* and the still later changes made for the 1746 Seasons.  ... 

Page 37 of RE:view Vol. 6, Issue 2 [page]

1974 RE:view  
Editing for large type reproduction was not as extensive as for braille reproduction.  ...  The next task in the adaptation of these tests was the editing of them for braille and large type reproduction.  ... 

Armature windings of direct-current dynamos

W.
1902 Journal of the Franklin Institute  
Third edition, revised and enlarged; pp. 952. New York : Electrical World and Engineer, N.D. Armature Windines of Direct-Current Dynamos : Extension and application of a ~eneral winding rule.  ...  types -but also affords a general solution of the winding problem.  ... 
doi:10.1016/s0016-0032(02)90318-2 fatcat:l2fjkvtdongnrnyk22dslfm5mi
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