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Would you want to know? Public attitudes on early diagnostic testing for Alzheimer's disease
2013
Alzheimer's Research & Therapy
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Would they want to know whether they will get this fatal, untreatable disease? ...
Third, future surveys should measure whether people would want to take such a test if they were told that no treatment or cure is currently available. ...
doi:10.1186/alzrt206
pmid:24010759
pmcid:PMC3978817
fatcat:pvxh6772onb2phxaznd5odcsqa
Metabolomics in Advanced Liver Disease
2021
Current treatment options in gastroenterology
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Elizabeth J. Want declares that she has no conflict of interest. Mark JW McPhail declares that he has no conflict of interest. ...
Equal
Declaration
Ethics approval
N/A
Liver (J Bajaj, Section Editor) ...
doi:10.1007/s11938-021-00347-w
fatcat:62he6q5rzrfojbcldzoc3kjaum
"I just want to be skinny.": A content analysis of tweets expressing eating disorder symptoms
2019
PLoS ONE
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In addition to examining DSM ED criteria within tweets, we wanted to determine if a social analytics company could be used to successfully infer the demographics of Twitter users that would align with ...
doi:10.1371/journal.pone.0207506
pmid:30650072
pmcid:PMC6334988
fatcat:6iygty7al5hbpmy3g54vtsjssi
Does 'Wanting the Best' create more stress? The link between baby sign classes and maternal anxiety
2010
Infant and Child Development
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Does 'wanting the best' create more stress? The link between baby sign classes and maternal anxiety. ...
As long as there are parents who want the very best for their child, there will be companies keen to offer a vast range of products to promote the child's development. ...
doi:10.1002/icd.705
fatcat:micku7fl3nafjnkumn2qbndjyu
Will not want: Self-control rather than motivation explains the female advantage in report card grades
2015
Learning and Individual Differences
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Put succinctly, will, not want, explains the superior performance of girls in the classroom. ...
Do girls simply want to do well in school more than boys do, finding it either more interesting or important (i.e., a difference in motivation)? ...
doi:10.1016/j.lindif.2015.02.006
pmid:25883522
pmcid:PMC4395866
fatcat:5ae67hg2qfgzbgjhn6updsgrvu
The Expanding Role of Mass Spectrometry in Metabolite Profiling and Characterization
2005
ChemBioChem
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A Microdialysis Workflow for Metabotyping Superficial Pathologies: Application to Burn Injury
2019
Analytical Chemistry
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ORCID Elizabeth J. Want: 0000-0002-7433-8632 Notes The authors declare no competing financial interest. ■ ACKNOWLEDGMENTS E.J.W. acknowledges Waters Corporation for funding. ...
doi:10.1021/acs.analchem.8b05615
pmid:31021084
pmcid:PMC6533596
fatcat:xrkd5vxcgrbbtbzejl7v7ftiie
Can an old dog learn (and want to experience) new tricks? Cognitive training increases openness to experience in older adults
2012
Psychology and Aging
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The present study investigated whether an intervention aimed to increase cognitive ability in older adults also changes the personality trait of openness to experience. Older adults completed a 16-week program in inductive reasoning training supplemented by weekly crossword and Sudoku puzzles. Changes in openness to experience were modeled across four assessments over 30 weeks using latent growth curve models. Results indicate that participants in the intervention condition increased in the
doi:10.1037/a0025918
pmid:22251379
pmcid:PMC3330146
fatcat:z4tagszqhvgxfnogguvbby2k2u
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... t of openness compared with a waitlist control group. The study is one of the first to demonstrate that personality traits can change through nonpsychopharmocological interventions.
From Samples to Insights into Metabolism: Uncovering Biologically Relevant Information in LC-HRMS Metabolomics Data
2019
Metabolites
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Untargeted metabolomics (including lipidomics) is a holistic approach to biomarker discovery and mechanistic insights into disease onset and progression, and response to intervention. Each step of the analytical and statistical pipeline is crucial for the generation of high-quality, robust data. Metabolite identification remains the bottleneck in these studies; therefore, confidence in the data produced is paramount in order to maximize the biological output. Here, we outline the key steps of
doi:10.3390/metabo9120308
pmid:31861212
pmcid:PMC6950334
fatcat:6anqngosy5a6jgi5bjhyfdabpu
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... e metabolomics workflow and provide details on important parameters and considerations. Studies should be designed carefully to ensure appropriate statistical power and adequate controls. Subsequent sample handling and preparation should avoid the introduction of bias, which can significantly affect downstream data interpretation. It is not possible to cover the entire metabolome with a single platform; therefore, the analytical platform should reflect the biological sample under investigation and the question(s) under consideration. The large, complex datasets produced need to be pre-processed in order to extract meaningful information. Finally, the most time-consuming steps are metabolite identification, as well as metabolic pathway and network analysis. Here we discuss some widely used tools and the pitfalls of each step of the workflow, with the ultimate aim of guiding the reader towards the most efficient pipeline for their metabolomics studies.
Assignment of Endogenous Substrates to Enzymes by Global Metabolite Profiling†
2004
Biochemistry
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The product was then eluted using 2% HCl in methanol to afford C18:1 NAT (15 mg, 23%): 1 H NMR (500 MHz, CDCl 3 ) δ 5.34 (m, 2H), 3.66 (t, 2H, J ) 7 Hz), 3.00 (t, 2H, J ) 7 Hz), 2.31 (t, 2H, J ) 7.7 Hz ...
), 2.03 (m, 4H), 1.62 (m, 2H), 1.31 (m, 20 H), 0.90 (t, 3H, J ) 6.95 Hz). ...
doi:10.1021/bi0480335
pmid:15533037
fatcat:6h6qqwvyifcozp3n77rdy4czfq
Phospholipid capture combined with non-linear chromatographic correction for improved serum metabolite profiling
2006
Metabolomics
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All solvents, including the water for the LC/MS studies were HPLC grade (J. T. Baker, Phillipsburg, NJ). ...
., 2003; Want et al., 2006) . However, these approaches are not particularly effective at removing phospholipids. ...
doi:10.1007/s11306-006-0028-0
fatcat:2q5fth4cmvhuzhroleneacnmae
A Statistically Rigorous Test for the Identification of Parent−Fragment Pairs in LC-MS Datasets
2010
Analytical Chemistry
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O.; Zhang, O.; Page, J. S.; Shen, Y.; Callister, S. J.; Jacobs, J. M.; Smith, R. D. Biomark. Med. 2007, 1 (1), 159-185. (4) Want, E. J.; Nordstrom, A.; Morita, H.; Siuzdak, G. J. ...
A.; Want, E. J.; O'Maille, G.; Abagyan, R.; Siuzdak, G. Anal. Chem. 2006, 78, 779-787. (29) Lommen, A. Anal. Chem. 2009, 81 (8), 3079-3086. (30) Katajamaa, M.; Miettinen, J.; Oresic, M. ...
doi:10.1021/ac902361f
pmid:20143830
pmcid:PMC2829950
fatcat:xglbf3bznvcmvghyznyjwdk2uy
Untargeted UPLC-MS Profiling Pipeline to Expand Tissue Metabolome Coverage: Application to Cardiovascular Disease
2015
Analytical Chemistry
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Metabolic profiling studies aim to achieve broad metabolome coverage in specific biological samples. However, wide metabolome coverage has proven difficult to achieve, mostly because of the diverse physicochemical properties of small molecules, obligating analysts to seek multiplatform and multimethod approaches. Challenges are even greater when it comes to applications to tissue samples, where tissue lysis and metabolite extraction can induce significant systematic variation in composition. We
doi:10.1021/ac503775m
pmid:25664760
pmcid:PMC4407508
fatcat:k52aujy2hzgqnbsbziju2noy7m
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... have developed a pipeline for obtaining the aqueous and organic compounds from diseased arterial tissue using two consecutive extractions, followed by a different untargeted UPLC-MS analysis method for each extract. Methods were rationally chosen and optimized to address the different physicochemical properties of each extract: hydrophilic interaction liquid chromatography (HILIC) for the aqueous extract and reversed-phase chromatography for the organic. This pipeline can be generic for tissue analysis as demonstrated by applications to different tissue types. The experimental setup and fast turnaround time of the two methods contributed toward obtaining highly reproducible features with exceptional chromatographic performance (CV % < 0.5%), making this pipeline suitable for metabolic profiling applications. We structurally assigned 226 metabolites from a range of chemical classes (e.g., carnitines, α-amino acids, purines, pyrimidines, phospholipids, sphingolipids, free fatty acids, and glycerolipids) which were mapped to their corresponding pathways, biological functions and known disease mechanisms. The combination of the two untargeted UPLC-MS methods showed high metabolite complementarity. We demonstrate the application of this pipeline to cardiovascular disease, where we show that the analyzed diseased groups (n = 120) of arterial tissue could be distinguished based on their metabolic profiles. M etabolic profiling relies on the application of a range of analytical technologies to measure simultaneously differential levels of multiple metabolites in biological matrices. 1 It is important to ensure wide metabolome coverage and consequently enhance biomarker detection probability. For this an untargeted format is the approach of choice warranting the ability to detect unmapped metabolites and pathways, as well as compounds originating from environmental interactions unrelated to the biology of the system studied. 2,3 To compensate for the different capabilities of each technique/ method, their bias toward specific classes of metabolites, 4 and the wide physicochemical diversity of the metabolome in a biological matrix, multiple platforms 5−8 and methods 4 are required to expand metabolite coverage. Hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) is a relatively new chromatographic tool applied in the effort to expand metabolome coverage. The importance of HILIC is attributed to its ability to
Construction of Confidence Regions for Isotopic Abundance Patterns in LC/MS Data Sets for Rigorous Determination of Molecular Formulas
2010
Analytical Chemistry
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The development of a test of hypothesis with a known null distribution would be highly desirable, but for want of a detailed mathematical model which can rigorously account for the mixture of isotopologues ...
doi:10.1021/ac101278x
pmid:20690638
pmcid:PMC2930401
fatcat:6yylzv7bgrgolhw5f3yv3bawoy
Metabolic perturbations associated with the consumption of a ketogenic medium-chain TAG diet in dogs with idiopathic epilepsy
2018
British Journal of Nutrition
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AbstractConsumption of diets containing medium-chain TAG (MCT) has been shown to confer neuroprotective effects. We aim to identify the global metabolic perturbations associated with consumption of a ketogenic diet (medium-chain TAG diet (MCTD)) in dogs with idiopathic epilepsy. We used ultra-performance liquid chromatography-MS (UPLC-MS) to generate metabolic and lipidomic profiles of fasted canine serum and made comparisons between the MCTD and standardised placebo diet phases. We identified
doi:10.1017/s0007114518001617
pmid:30001753
fatcat:wlssw47dxnatxbq3mbiz4oiwji
more »
... etabolites that differed significantly between diet phases using metabolite fragmentation profiles generated by tandem MS (UPLC–MS/MS). Consumption of the MCTD resulted in significant differences in serum metabolic profiles when compared with the placebo diet, where sixteen altered lipid metabolites were identified. Consumption of the MCTD resulted in reduced abundances of palmitoylcarnitine, octadecenoylcarnitine, stearoylcarnitine and significant changes, both reduced and increased abundances, of phosphatidylcholine (PC) metabolites. There was a significant increase in abundance of the saturated C17 : 0 fatty acyl moieties during the MCTD phase. Lysophosphatidylcholine (17 : 0) (P=0·01) and PC (17:0/20:4) (P=0·03) were both significantly higher in abundance during the MCTD. The data presented in this study highlight global changes in lipid metabolism, and, of particular interest, in the C17 : 0 moieties, as a result of MCT consumption. Elucidating the global metabolic response of MCT consumption will not only improve the administration of current ketogenic diets for neurological disease models but also provides new avenues for research to develop better diet therapies with improved neuroprotective efficacies. Future studies should clarify the involvement and importance of C17 : 0 moieties in endogenous MCT metabolic pathways.
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