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Efficiency, Selectivity, and Robustness of Nucleocytoplasmic Transport

Anton Zilman, Stefano Di Talia, Brian T. Chait, Michael P. Rout, Marcelo O. Magnasco
2007 PLoS Computational Biology  
All materials enter or exit the cell nucleus through nuclear pore complexes (NPCs), efficient transport devices that combine high selectivity and throughput.  ...  We show that specific binding of transport factors to the FG-nups facilitates transport and provides the mechanism of selectivity.  ...  as FG nups, is a key feature of the transport process, and allows them to selectively and efficiently pass with their cargoes through the NPC.  ... 
doi:10.1371/journal.pcbi.0030125 pmid:17630825 pmcid:PMC1914370 fatcat:5zq6ksdadnd5zceqv2l3wndczq

Epigenetic Small Molecules Rescue Nucleocytoplasmic Transport and DNA Damage Phenotypes in C9ORF72 ALS/FTD

Melina Ramic, Nadja S. Andrade, Matthew J. Rybin, Rustam Esanov, Claes Wahlestedt, Michael Benatar, Zane Zeier
2021 Brain Sciences  
In addition to restoring nucleocytoplasmic transport efficiencies, hits from the screen also counteract the cytotoxic effects of PR.  ...  disruption of nucleocytoplasmic transport and increased DNA damage contributes to pathology.  ...  Acknowledgments: We thank the members of the Center for Therapeutic Innovation at the University of Miami Miller School of Medicine for their comments and critiques throughout this project.  ... 
doi:10.3390/brainsci11111543 pmid:34827542 pmcid:PMC8616043 fatcat:fkxzg4yfjzcbzdnwa56i4anohi

'Natively unfolded' nucleoporins in nucleocytoplasmic transport

Weidong Yang
2011 Nucleus  
T he nuclear pore complex (NPC) acts as a selective gate that mediates the bidirectional transport of macromolecules between the cytoplasm and the nucleus of eukaryotic cells.  ...  'Natively unfolded' nucleoporins (Nups) with domains rich in phenylalanine-glycine (FG) repeats form the selective permeability barrier and provide binding sites for mobile transport receptors in the NPC  ...  Acknowledgements We thank the foundation support from National Institute of Health (GM094041-01) and the Research Capacity Enhancement Grant (Bowling Green State University).  ... 
doi:10.4161/nucl.13818 pmid:21647294 pmcid:PMC3104804 fatcat:676d5lozlrg63l4ihh7zhx7zhu

Self-regulated viscous channel in the nuclear pore complex

J. Ma, A. Goryaynov, A. Sarma, W. Yang
2012 Proceedings of the National Academy of Sciences of the United States of America  
Nonetheless, due to our limited knowledge of the native configuration of the FG-Nup barrier and of the interactions of the transiting molecules with this barrier, the precise nucleocytoplasmic transport  ...  These FG-Nups form the selective permeability barrier in the NPC that allows for two transport modes: passive diffusion of small molecules (<40-60 kDa) and transport-receptor-facilitated translocation  ...  , efficient and robust nucleocytoplasmic transport in living cells.  ... 
doi:10.1073/pnas.1201724109 pmid:22529346 pmcid:PMC3358865 fatcat:zg6p6xgj2vfvzpd46ndf2truze

Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport

Laura J. Terry, Susan R. Wente
2009 Eukaryotic Cell  
This compartmentalization of eukaryotic cells allows spatial regulation of gene expression; however, it also necessitates a mechanism for transport between the nucleus and cytoplasm.  ...  The extensive repeats of phenylalanine-glycine (FG) in each FG-Nup directly bind to shuttling transport receptors moving through the NPC.  ...  ACKNOWLEDGMENT This study was supported by grant R01 GM051219 from the National Institutes of Health (S.R.W.).  ... 
doi:10.1128/ec.00225-09 pmid:19801417 pmcid:PMC2794212 fatcat:oevzogj3tveifkzmqyt5fdjvcm

Viral Subversion of Nucleocytoplasmic Trafficking

Melanie L. Yarbrough, Miguel A. Mata, Ramanavelan Sakthivel, Beatriz M. A. Fontoura
2013 Traffic : the International Journal of Intracellular Transport  
Conversely, many viruses co-opt nucleocytoplasmic trafficking to facilitate transport of viral RNAs.  ...  Trafficking of proteins and RNA into and out of the nucleus occurs through the nuclear pore complex (NPC).  ...  Acknowledgments We thank Angela Diehl for outstanding design of the figures.  ... 
doi:10.1111/tra.12137 pmid:24289861 pmcid:PMC3910510 fatcat:g6w35le7uve2jcbtmphctpikzy

Nucleocytoplasmic Transport

2008 Biophysical Journal  
Experimental observations have revealed that the NPC can maintain high rates of transport throughput for most cargo, regardless of the actual shape or, to within a limit, size of the cargo as long as the  ...  To get insight into the mechanism of cer-1-p function in cellular membranes and specifically its interaction with Ca 2+ , we present a detailed structure of monomolecular layers of cer-1-p at the airwater  ...  Cell Biol. 6, 197-206 (2004 Board B226 Large proteins and macromolecular complexes have to enter and leave the nucleus in an efficient and selective manner.  ... 
doi:10.1016/s0006-3495(08)79177-x fatcat:ftqppzldmzgilgpkx64edt4ghm

Kinetics of NF-κB nucleocytoplasmic transport probed by single-cell screening without imaging

Jun Wang, Bei Fei, Yihong Zhan, Robert L. Geahlen, Chang Lu
2010 Lab on a Chip  
Transport of protein and RNA cargoes between the nucleus and cytoplasm (nucleocytoplasmic transport) is vital for a variety of cellular functions.  ...  In this report, we describe using a novel method, microfluidic electroporative flow cytometry, to study kinetics of nucleocytoplasmic transport of an important transcription factor NF-kB.  ...  Acknowledgements We thank NSF CBET 1016547 and NIH NCI CA115465 for financial support of this research.  ... 
doi:10.1039/c0lc00094a pmid:20835431 pmcid:PMC2954252 fatcat:vxrwd43evbbsbiofex4iwaacwu

Nucleocytoplasmic transport of intrinsically disordered proteins studied by high-speed super-resolution microscopy

Samuel L. Junod, Joseph M. Kelich, Jiong Ma, Weidong Yang
2020 Protein Science  
A Ch/Hy-ratio mechanism was finally suggested for nucleocytoplasmic transport of IDPs.  ...  Furthermore, their diffusion efficiencies and routes are differentiated by their content ratio of charged (Ch) and hydrophobic (Hy) amino acids.  ...  The project was supported by grants from the US National Institutes of Health (NIH GM094041, GM097037, GM116204, and GM122552 to W.Y.).  ... 
doi:10.1002/pro.3845 pmid:32096308 fatcat:baqzdwfahvddtless7zp5da2im

Biology and Biophysics of the Nuclear Pore Complex and Its Components [chapter]

Roderick Y.H. Lim, Katharine S. Ullman, Birthe Fahrenkrog
2008 International Review of Cell and Molecular Biology  
Biophysical and imaging approaches have helped elucidate how nucleoporins act as a selective barrier in nucleocytoplasmic transport.  ...  Nucleocytoplasmic exchange of proteins and ribonucleoprotein particles occurs via nuclear pore complexes (NPCs) that reside in the double membrane of the nuclear envelope (NE).  ...  By solving a rigorous mathematical model of transport through the NPC, Zilman et al. showed that selectivity, efficiency, directionality, and robustness of nucleocytoplasmic transport could be explained  ... 
doi:10.1016/s1937-6448(08)00632-1 pmid:18544502 pmcid:PMC4366138 fatcat:zyu3adx2bzcqhevpaktillnd5q

The Ran GTPase System in Fission Yeast Affects Microtubules and Cytokinesis in Cells That Are Competent for Nucleocytoplasmic Protein Transport

S. S. Salus, J. Demeter, S. Sazer
2002 Molecular and Cellular Biology  
Microtubule function, cytokinesis, and nuclear envelope structure are the Ran-dependent processes most sensitive to the amount of Ran protein in the cell, whereas nucleocytoplasmic protein transport is  ...  the most robust.  ...  studies on nucleocytoplasmic transport in the pim1 mutant collection; Kathy Gould for the GFP-cdc4 fusion constructs and the anti-Arp3 antibody; Keith Gull for the TAT-1 antibody; and Ursula Fleig, Xiangwei  ... 
doi:10.1128/mcb.22.24.8491-8505.2002 pmid:12446769 pmcid:PMC139886 fatcat:ggbugoa5wndt7nb2dwdmtfuhlu

Probing the nucleoporin FG repeat network defines structural and functional features of the nuclear pore complex

Philipp Stelter, Ruth Kunze, Jessica Fischer, Ed Hurt
2011 Journal of Cell Biology  
These data suggest some dominant effect of these NupeDID constructs with respect to growth and/or nucleocytoplasmic transport.  ...  S3 shows analysis of growth and nucleocytoplasmic transport of NUP-eDID + pGAL-DYN2 strains transformed with the respective wild-type Nups.  ... 
doi:10.1083/jcb.201105042 pmid:21987633 pmcid:PMC3198172 fatcat:4eqozsuopfed7jyigyq4vg2c3i

Higher Nucleoporin-Importinβ Affinity at the Nuclear Basket Increases Nucleocytoplasmic Import

Mohammad Azimi, Mohammad R. K. Mofrad, Ivano Eberini
2013 PLoS ONE  
To shed light on the affinity gradient theory and better characterize how the existence of such an affinity gradient between the nuclear pore and the import receptor may influence the nucleocytoplasmic  ...  Several in vitro studies have shown the presence of an affinity gradient in nuclear pore complex proteins for the import receptor Importinb, at least partially contributing to nucleocytoplasmic transport  ...  of Molecular Cell Biomechanics Laboratory.  ... 
doi:10.1371/journal.pone.0081741 pmid:24282617 pmcid:PMC3840022 fatcat:567titwhfbbnpalsyygo5iqdr4

Constitutive p38 activation caused inactivation of CRE transcription is mediated by hyper-phosphorylation dependent CRTC2 nucleocytoplasmic transport

Huabin Ma, Zeyuan Liu, Chuan-Qi Zhong, Yifei Liu, Zhirong Zhang, Yaoji Liang, Jingxian Li, Shoufa Han, Jiahuai Han
2019 Molecular and Cellular Biology  
Collectively, our study uncovered a previously unknown mechanism of inactivation of selected transcription, which is resulted from hyper-phosphorylation driven nucleocytoplasmic transport of co-factors  ...  The hyper-phosphorylation driven nucleocytoplasmic transport of CRTC2 may not be a rare case of nuclear export of proteins, as we also observed that constitutively activated p38 promoted FOS nuclear export  ...  ACKNOWLEDGMENTS We thank Lu Zhou (Xiamen University) for critical reading of the manuscript. We thank Wei Han (Xiamen University) for providing cDNAs of cofactors.  ... 
doi:10.1128/mcb.00554-18 pmid:30782776 pmcid:PMC6469919 fatcat:hhcombo2knenjks55ngtsruv34

Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease-The Example of C9orf72 ALS

Marije F W Semmelink, Anton Steen, Liesbeth M Veenhoff
2021 International Journal of Molecular Sciences  
Taking a specific type of ALS caused by mutations in C9orf72 as an example, we illustrate these complications, and discuss how the current data do not firmly answer whether the kinetics of nucleocytoplasmic  ...  In this review, we summarize how nuclear transport works, which transport assays are available, and what matters complicate the interpretation of their results.  ...  Introduction Nucleocytoplasmic Transport Nucleocytoplasmic transport (NCT) occurs through nuclear pore complexes (NPCs), large protein complexes embedded in the nuclear membrane.  ... 
doi:10.3390/ijms22179217 pmid:34502125 pmcid:PMC8431710 fatcat:zuby5rj64ng4bmqwa43gynpjfe
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