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Analysis of membrane bilayer asymmetry using parinaric acid fluorescent probes

Larry A. Sklar, Edward A. Dratz
1980 FEBS Letters  
Acknowledgements This work was supported in part by USPH grant EY00175 (to E.A.D.) and a Postdoctoral Fellowship from the Helen Hay Whitney Foundation (to L.A.S.).  ...  This mixture exhibited a thermal phase separation beginning -5°C higher than a typical preparation of disc phosphatidylcholine [5, 7] .  ...  The PnA polarization in a dispersion of the total membrane lipids (not shown) has a temperature range which is midway between the dispersions that are models for the 'outer' and 'inner' layers and does  ... 
doi:10.1016/0014-5793(80)80245-6 pmid:7418878 fatcat:5jzgoueghna5hkalx7thwl3jzq

Deuteron NMR study of ferroelectric transition in VF2/TrFE copolymer

V. Hugo Schmidt, Carole Perry, Edward A. Dratz, Yong Ke
1991 Ferroelectrics (Print)  
A calculation is presented for the deuteron quadrupolar NMR spin-lattice relaxation caused by kink-+bond motions along disordered helical chains in the paraelectric phase.  ...  Deuteron NMR results on a 70/30 mole Ri random copolymer of deuterated vinylidene fluoride and normal trifluoroethylene are reviewed, for temperatures on both sides of the ferroelectric-paraelectric phase  ...  PVFn, is a polymer which exhibits a strong and commercially important piezoelectric effect.  ... 
doi:10.1080/00150199108222412 fatcat:evvsonot3fbrfmoioif5qg6vge

Revealing protein structures

Brendan M. Mumey, Brian W. Bailey, Edward A. Dratz
2002 Proceedings of the sixth annual international conference on Computational biology - RECOMB '02  
A recent idea for determining the three-dimensional structure of a protein uses antibody recognition of surface structure and random peptide libraries to map antibody epitope combining sites.  ...  The binding site of an antibody is a surface, not just a linear sequence, so the epitope mapping alignment problem is outside the scope of classical string alignment algorithms, such as Smith-Waterman.  ...  Input: A probe string s, a target string t (over a common alphabet), a substitution score matrix M, a distance penalty function d, an objective score Q.  ... 
doi:10.1145/565196.565226 dblp:conf/recomb/MumeyBD02 fatcat:cazfci4ecnacdj4w37mwlo3rym

NMR structure of a receptor-bound G-protein peptide

Edward A. Dratz, Julie E. Furstenau, Christophe G. Lambert, Dennis L. Thireault, Helen Rarick, Theresa Schepers, Sergei Pakhlevaniants, Heidi E. Hamm
1997 Nature  
A. Leonard and V. A. Best for technical assistance.  ...  determined as described 28 Nature 363 , 363 276-280 (1993) .................................................................................................................................. Ⅺ 1 . 1 Dratz  ...  was without effect on adenosine A 1 or A 2 receptors.  ... 
doi:10.1038/37165 fatcat:swiaddsuvfbndeps5eqokwruzq

Antibody Imprint of a Membrane Protein Surface

James B. Burritt, Scott C. Busse, Dawit Gizachew, Daniel W. Siemsen, Mark T. Quinn, Clifford W. Bond, Edward A. Dratz, Algirdas J. Jesaitis
1998 Journal of Biological Chemistry  
PHE_5:HE a VAL_10:HG a 2.5 3.5 2. PHE_5:HD a VAL_10:HG a 2.5 3.5 3. PHE_5:HN THR_1:HN 2.5 3.5 4. PHE_5:HD a THR_1:HG a 2.5 3.5 5. PHE_5:HE a THR_1:HG a 2.5 3.5 6. PHE_5:HN THR_1:HG a 2.5 3.5 7.  ...  Spectra A and B were collected using a standard two-dimensional NOESY pulse sequence with a watergate solvent suppression and a 300 ms mixing time.  ...  Bond, Edward A. Dratz  ... 
doi:10.1074/jbc.273.38.24847 pmid:9733789 fatcat:jvm7tyt7rzgindgavl5m6t6mry

Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions

Gal Keren-Aviram, Fabien Dachet, Shruti Bagla, Karina Balan, Jeffrey A. Loeb, Edward A. Dratz, Giuseppe Biagini
2018 PLoS ONE  
This study is unique in that for each of these six patients the comparison of protein expression was made within the same patient: a more epileptic region was compared to a less epileptic brain region.  ...  A total of 397 protein spots of interest (SOI) were clustered by protein expression patterns across all samples.  ...  Writing -original draft: Gal Keren-Aviram, Jeffrey A. Loeb, Edward A. Dratz. Writing -review & editing: Gal Keren-Aviram, Fabien Dachet, Jeffrey A. Loeb, Edward A.  ... 
doi:10.1371/journal.pone.0195639 pmid:29634780 pmcid:PMC5892923 fatcat:gz3z44lf2jelxnrrpdz42bnyvi

Interpretation of 100- and 360-MHz proton magnetic resonance spectra of retinal rod outer segment disk membranes

Michael F. Brown, George P. Miljanich, Edward A. Dratz
1977 Biochemistry  
The spectra of both the ROS membranes and bilayer vesicles prepared from purified ROS phospholipids (liposomes) appear to represent a superposition of relatively sharp resonance components and a broad,  ...  A. Dratz, unpublished) .  ...  A. Dratz, in preparation) .  ... 
doi:10.1021/bi00631a010 pmid:889780 fatcat:pwghha5rljgctelgnyh35gvmuy

Elevating optimal human nutrition to a central goal of plant breeding and production of plant-based foods

David C. Sands, Cindy E. Morris, Edward A. Dratz, Alice L. Pilgeram
2009 Plant Science  
In this way we might circumvent a natural tendency for high yield and low production cost to effectively select against the best human nutrition.  ...  However, nutrition is currently not an important driver of most plant breeding efforts, and there are only a few well-known efforts to breed crops that are adapted to the needs of optimal human nutrition  ...  Acknowledgments We are grateful for the thoughtful remarks and suggestions of the seven reviewers, the editor, Constance Dratz, and our students, Anne Runkel and Brekke Petersen.  ... 
doi:10.1016/j.plantsci.2009.07.011 pmid:20467463 pmcid:PMC2866137 fatcat:453burzugnajvbvthtjk7jxshy

A New Method for Mapping Discontinuous Antibody Epitopes to Reveal Structural Features of Proteins

Brendan M. Mumey, Brian W. Bailey, Bonnie Kirkpatrick, Algirdas J. Jesaitis, Thomas Angel, Edward A. Dratz
2003 Journal of Computational Biology  
This paper presents a new method to align these antibody epitopes to discontinuous regions of the one-dimensional amino acid sequence of a target protein.  ...  The binding site of an antibody is a surface, not just a continuous linear sequence, so the epitope mapping alignment problem is outside the scope of classical string alignment algorithms, such as Smith-Waterman  ...  (Bailey and Dratz, unpublished) .  ... 
doi:10.1089/10665270360688183 pmid:12935344 fatcat:pxybraomvze33c3nfqn2pjiyfm

Thermotropic behavior of retinal rod membranes and dispersions of extracted phospholipids

George P. Miljanich, Michael F. Brown, Susan Mabrey-Gaud, Edward A. Dratz, Julian M. Sturtevant
1985 Journal of Membrane Biology  
ROS disk membranes were found to exhibit a broad peak of excess heat capacity with a maximum at less than about 3~ ascribable to a gel-to-liquid crystalline phase transition of a fraction of the phosphofipids  ...  A similar lhermotropic transition was observed for aqueous dispersions of the total extracted and purified ROS phosphofipids.  ...  ., 1976; Brown, Deese & Dratz, 1982) .  ... 
doi:10.1007/bf01872007 pmid:4020856 fatcat:co6w3ntccvfk3expubsvoxjjmm

1H-NMR studies of protein-lipid interactions in retinal rod outer segment disc membranes

Michael F. Brown, George P. Miljanich, Lawrence K. Franklin, Edward A. Dratz
1976 FEBS Letters  
Dratz, in preparation) indicate that our preparations contain < 2% by weight cholesterol in total ROS lipids. North-Holland Publishing Company -Amsterdam  ...  Supported by a Fight for Sight Fellowship ** Recent analyses in our laboratory (W. L. Stone and E. A.  ... 
doi:10.1016/0014-5793(76)80725-9 pmid:992076 fatcat:bsmyxamdjrgb7dqnw7rlrjym7m

A carboxyl-terminal tail peptide of neutrophil chemotactic receptor disrupts its physical complex with G protein

Rajani K. Bommakanti, Karl-Norbert Klotz, Edward A. Dratz, Algirdas J. Jesaitis
1993 Journal of Leukocyte Biology  
The peptide competition was assayed by dissociation of a GTP-sensitive, rapidly sedimenting (78) form of receptor-G protein compla as analyzed by velocity sedimentation on linear sucrose density gradienta  ...  A control peptide, with the reverse sequence, rCTUl ( 336 TATDST(lfSDETLAR' 2 2), did not perturb the sedimentation of the reconstituted receptor-G protein complex up to the highest tested concentration  ...  This conversion occurred with a GTP"YS EC 50 of approximately 20 nM and appeared to correlate with a reduction in sedimentation rate of G a and ß"Y subunits [18) .  ... 
doi:10.1002/jlb.54.6.572 pmid:8245709 fatcat:jfvx2czvqzbm3ohmwuh775jptm

The Quantum Biology of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics

Robert J. Usselman, Cristina Chavarriaga, Pablo R. Castello, Maria Procopio, Thorsten Ritz, Edward A. Dratz, David J. Singel, Carlos F. Martino
2016 Scientific Reports  
Here, we present results that reveal a novel domain of quantum biology: the control of the biological production of reactive oxygen species (ROS) via coherent spin dynamics in a radical pair (RP) reaction  ...  Either O 2 •− is released from the triplet RP state and escapes into the medium, or a hydroperoxy-flavin transient  ...  In the perpendicular orientation, we measured a 36% ± 4 reduction of O 2 •− (p < 0.002) and a 21% ± 5 reduction of H 2 O 2 (p < 0.02).  ... 
doi:10.1038/srep38543 pmid:27995996 pmcid:PMC5172244 fatcat:j2krsiel4vch7fqzy246mcnf7a

Predicting novel histopathological microlesions in human epileptic brain through transcriptional clustering

Fabien Dachet, Shruti Bagla, Gal Keren-Aviram, Andrew Morton, Karina Balan, Laleh Saadat, Tibor Valyi-Nagy, William Kupsky, Fei Song, Edward Dratz, Jeffrey A. Loeb
2014 Brain  
, a marker of epileptic activity, in superficial layers.  ...  We developed a method to identify cellular changes in human epileptic neocortex using transcriptional clustering.  ...  Using a statistical clustering algorithm, we found a subgroup of the differentially-expressed genes arranged into 11 tight clusters, each predicting a change in a specific cell type.  ... 
doi:10.1093/brain/awu350 pmid:25516101 pmcid:PMC4306820 fatcat:vnolbmblyvhuhje2btqz4sstra

Retinal rod outer segment lipids form bilayers in the presence and absence of rhodopsin: a31P NMR study

Alan J. Deese, Edward A. Dratz, Michael F. Brown
1981 FEBS Letters  
A. D. During the preliminary stages of this work, M. F.  ...  A. J. D. was supported during part of this work by the US National Institutes of Health (5SO7 RR 07135-10).  ...  behaviour'; and 'Rhodopsin, being the predominant membrane protein (>85%, w/w), must play a decisive role in organizing the lipids into a bilayer'.  ... 
doi:10.1016/0014-5793(81)80061-0 pmid:7215559 fatcat:c7rtzdzqvbhdveyurpqg24ltwu
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