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Malaria: A 21st century solution for an ancient disease

Dyann Wirth
1998 Nature Medicine  
DYANN WIRTH 1360 NATURE MEDICINE • VOLUME 4 • NUMBER 12 • DECEMBER 1998 NEWS & VIEWS drugs promoting the recruitment of opioid-producing cells.  ... 
doi:10.1038/3943 pmid:9846567 fatcat:gewhsjnxubfhdbhhc6ddqrjdq4

Global action for training in malaria elimination

Dyann F. Wirth, Núria Casamitjana, Marcel Tanner, Michael R. Reich
2018 Malaria Journal  
The Rethinking Malaria Leadership Forum, held at Harvard Business School in February 2017 with collaboration of the Barcelona Institute for Global Health and the Swiss Tropical and Public Health Institute, identified this training gap as a high priority for both analysis and action. The gap in human resource training for malaria elimination needs to be addressed in order to assure continued progress. This paper identifies major gaps in skills and human resources, suggests institutions that can
more » ... ssist in filling the training gaps, and proposes global actions to implement expanded training for malaria elimination in endemic countries.
doi:10.1186/s12936-018-2199-3 pmid:29370810 pmcid:PMC5785838 fatcat:oynac7q4dneove4lw5p2vl2fgm

The cloned rRNA genes ofP. lophurae:a novel rDNA structure

Thomas R. Unnasch, Dyann F. Wirth
1983 Nucleic Acids Research  
This work was supported by a grant to Dyann F. Wirth from the National Institutes of Health. Dyann F.  ...  Wirth is a recipient of a special award in molecular parasitology from the Burroughs Wellcome Foundation. Thomas R.  ... 
doi:10.1093/nar/11.23.8461 pmid:6324085 pmcid:PMC326595 fatcat:o2pfynr42baqjpsqyfrebkfeki

MOESM1 of hmmIBD: software to infer pairwise identity by descent between haploid genotypes

Stephen Schaffner, Aimee Taylor, Wesley Wong, Dyann Wirth, Daniel Neafsey
2018 Figshare  
Additional file 1. The hidden Markov model of hmmIBD. Mathematical details of the model behind hmmIBD.
doi:10.6084/m9.figshare.6274361.v1 fatcat:cqixoe63bfayzaboopqriccfri

hmmIBD: software to infer pairwise identity by descent between haploid genotypes [article]

Stephen F. Schaffner, Aimee R. Taylor, Wesley Wong, Dyann F. Wirth, Daniel E. Neafsey
2017 biorxiv/medrxiv   pre-print
We introduce hmmIBD, software to estimate pairwise identity by decent between haploid genomes, such as those of the malaria parasite, sampled from one or more populations. We verified hmmIBD using simulated data, benchmarked it against a previously published method for detecting IBD within populations, and demonstrated its utility using Plasmodium falciparum data from Cambodia and Ghana. Availability and Implemetation: Source code written in C99/C11-compliant C and requiring no external
more » ... s, is freely available for download at https://github.com/glipsnort/hmmIBD/releases, alongside test datasets.
doi:10.1101/188078 fatcat:eyyerpxayjbqjaxnfjfwedtz5e

MOESM3 of hmmIBD: software to infer pairwise identity by descent between haploid genotypes

Stephen Schaffner, Aimee Taylor, Wesley Wong, Dyann Wirth, Daniel Neafsey
2018 Figshare  
Additional file 3. Comparative study of isoRelate and hmmIBD and impact of assumed uniform recombination under hmmIBD. Full details and results of comparative study and exploration of the impact of assumed uniform recombination under hmmIBD.
doi:10.6084/m9.figshare.6274397 fatcat:jwnfezs7sbdf3c6lbpsh7zmbwm

Within-infection diversity of Plasmodium falciparum antigens reflects host-mediated selection [article]

Angela M. Early, Marc Lievens, Bronwyn MacInnis, Christian F. Ockenhouse, Sarah K. Volkman, Dyann F. Wirth, Daniel E. Neafsey
2017 bioRxiv   pre-print
Aimee Taylor, Seth Redmond, and members of the Neafsey and Wirth research groups provided thoughtful comments and discussions.  ... 
doi:10.1101/212209 fatcat:znmn7de3x5etdjnsdkcf53mc2y

MOESM2 of Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing

Angela Early, Rachel Daniels, Timothy Farrell, Jonna Grimsby, Sarah Volkman, Dyann Wirth, Bronwyn MacInnis, Daniel Neafsey
2019 Figshare  
Additional file 2. Parallel amplicon sequencing error correction (PASEC) workflow.
doi:10.6084/m9.figshare.8426366.v1 fatcat:uvveyh3mhbbmdesawosftpkqhu

MOESM1 of Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing

Angela Early, Rachel Daniels, Timothy Farrell, Jonna Grimsby, Sarah Volkman, Dyann Wirth, Bronwyn MacInnis, Daniel Neafsey
2019 Figshare  
Additional file 1. Additional tables and figures.
doi:10.6084/m9.figshare.8426357 fatcat:bunpikiayff4vob565hvzay2z4

hmmIBD: software to infer pairwise identity by descent between haploid genotypes

Stephen F. Schaffner, Aimee R. Taylor, Wesley Wong, Dyann F. Wirth, Daniel E. Neafsey
2018 Malaria Journal  
We introduce hmmIBD, software to estimate pairwise identity by decent between haploid genomes, such as those of the malaria parasite, sampled from one or more populations. We verified hmmIBD using simulated data, benchmarked it against a previously published method for detecting IBD within populations, and demonstrated its utility using Plasmodium falciparum data from Cambodia and Ghana. Availability and Implemetation: Source code written in C99/C11-compliant C and requiring no external
more » ... s, is freely available for download at https://github.com/glipsnort/hmmIBD/releases, alongside test datasets. Contact: sfs@broadinstitute.org Supplementary information: Supplementary data include Appendices S1, S2 and S3, and are available online. Wootton,J.C. et al. (2002) Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. 418, 18-21.
doi:10.1186/s12936-018-2349-7 pmid:29764422 pmcid:PMC5952413 fatcat:zyoazbsgbrejtogpdcjd3lidlm

Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence

Samuel I. Miller, Scott M. Landfear, Dyann F. Wirth
1986 Nucleic Acids Research  
At the 5' end of this transcript is the 35 nucleotide sequence that is present on the 5' end of Leishmania a-and B-tubulin mRNAs (Landfear, Miller, and Wirth; Molecular and Biochemical Parasitology in  ...  Recently, we (Landfear, Miller, and Wirth; Molecular and Biochemical Parasitology in press) have demonstrated that the first 35 nucleotides at the 5' end of a-and $-tubulin mRNAs are identical to bases  ... 
doi:10.1093/nar/14.18.7341 pmid:2429261 pmcid:PMC311755 fatcat:k6inwgwerrfa3cxxk2ppgllh34

Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing [article]

Angela M. Early, Rachel F. Daniels, Timothy M. Farrell, Jonna Grimsby, Sarah K. Volkman, Dyann F. Wirth, Bronwyn L. MacInnis, Daniel E. Neafsey
2018 bioRxiv   pre-print
Acknowledgements We thank Anita Lerch for thoughtful comments on the manuscript, Katelyn Durfee for help preparing the mock samples, and members of the Neafsey and Wirth labs for helpful discussions throughout  ... 
doi:10.1101/453472 fatcat:xqgg7qlllbb2hcr46kutzjem5u

De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria [article]

Seth N. Redmond, Bronwyn M. MacInnis, Selina Bopp, Amy K. Bei, Daouda Ndiaye, Daniel L. Hartl, Dyann F. Wirth, Sarah K. Volkman, Daniel E. Neafsey
2017 bioRxiv   pre-print
Funding for this work at the 568 Harvard TH Chan School of Public Health and the Broad Institute was provided by a 569 grant to DF Wirth from the Global Health Program at the Bill and Melinda Gates 570  ... 
doi:10.1101/213397 fatcat:r7xg5zwwubezxi6nudm62hk2je

Development of a fluorescence polarization based assay for histone deacetylase ligand discovery

Ralph Mazitschek, Vishal Patel, Dyann F. Wirth, Jon Clardy
2008 Bioorganic & Medicinal Chemistry Letters  
Histone deacetylases (HDACs) regulate many important physiological processes and the discovery of small molecules that modulate HDAC activity has both academic and clinical relevance. HDAC inhibitors, most notably SAHA, have been pursued as cancer chemotherapeutics but may be useful in treating psychiatric disorders, malaria, and other diseases. Herein, we describe an inexpensive and robust assay, based on fluorescence polarization, for HDAC ligand discovery. The assay is well suited for
more » ... roughput screening and enzyme kinetic studies. † contributed equally Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers
doi:10.1016/j.bmcl.2008.04.007 pmid:18430569 pmcid:PMC2408937 fatcat:q5mpq4x72rhg7ddtlatf4zltlm

Inactivation of Plasmepsins 2 and 3 Sensitizes Plasmodium falciparum to the Antimalarial Drug Piperaquine

Angana Mukherjee, Dominic Gagnon, Dyann F. Wirth, Dave Richard
2018 Antimicrobial Agents and Chemotherapy  
Dihydroartemisinin-piperaquine (DHA-PPQ), the current frontline artemisinin combination therapy used to treat Plasmodium falciparum malaria in multiple Southeast Asian countries, is now increasingly failing in Cambodia, where artemisinin resistance is nearly fixed, which suggests that PPQ resistance has emerged and is spreading rapidly in the Greater Mekong Subregion. Recent reports have shown that amplification of the genes encoding plasmepsins 2 and 3 is a molecular marker of PPQ resistance;
more » ... owever, whether these enzymes play a role in the mechanism of resistance is currently unknown. We show here that inactivating the genes encoding plasmepsin 2 or 3 individually in P. falciparum reference strain 3D7 results in hypersusceptibility to PPQ. Interestingly, no significant differences in the susceptibility to other antimalarials were observed, which suggests specific roles of plasmepsins 2 and 3 in PPQ susceptibility. The piperaquine hyper-sensitivity of the plasmepsin-2-and-3-inactivated lines provides direct evidence that these enzymes modulate parasite susceptibility to PPQ in the context of a single copy of PfMDR1 and independent of Kelch13 mutations conferring artemisinin resistance.
doi:10.1128/aac.02309-17 pmid:29439977 fatcat:sfd7325cvjdq3mpvyx64bx6zmy
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