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Malaria: A 21st century solution for an ancient disease
1998
Nature Medicine
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DYANN WIRTH 1360 NATURE MEDICINE • VOLUME 4 • NUMBER 12 • DECEMBER 1998
NEWS & VIEWS drugs promoting the recruitment of opioid-producing cells. ...
doi:10.1038/3943
pmid:9846567
fatcat:gewhsjnxubfhdbhhc6ddqrjdq4
Global action for training in malaria elimination
2018
Malaria Journal
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The Rethinking Malaria Leadership Forum, held at Harvard Business School in February 2017 with collaboration of the Barcelona Institute for Global Health and the Swiss Tropical and Public Health Institute, identified this training gap as a high priority for both analysis and action. The gap in human resource training for malaria elimination needs to be addressed in order to assure continued progress. This paper identifies major gaps in skills and human resources, suggests institutions that can
doi:10.1186/s12936-018-2199-3
pmid:29370810
pmcid:PMC5785838
fatcat:oynac7q4dneove4lw5p2vl2fgm
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... ssist in filling the training gaps, and proposes global actions to implement expanded training for malaria elimination in endemic countries.
The cloned rRNA genes ofP. lophurae:a novel rDNA structure
1983
Nucleic Acids Research
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This work was supported by a grant to Dyann F. Wirth from the National Institutes of Health. Dyann F. ...
Wirth is a recipient of a special award in molecular parasitology from the Burroughs Wellcome Foundation. Thomas R. ...
doi:10.1093/nar/11.23.8461
pmid:6324085
pmcid:PMC326595
fatcat:o2pfynr42baqjpsqyfrebkfeki
MOESM1 of hmmIBD: software to infer pairwise identity by descent between haploid genotypes
2018
Figshare
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Additional file 1. The hidden Markov model of hmmIBD. Mathematical details of the model behind hmmIBD.
doi:10.6084/m9.figshare.6274361.v1
fatcat:cqixoe63bfayzaboopqriccfri
hmmIBD: software to infer pairwise identity by descent between haploid genotypes
[article]
2017
biorxiv/medrxiv
pre-print
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We introduce hmmIBD, software to estimate pairwise identity by decent between haploid genomes, such as those of the malaria parasite, sampled from one or more populations. We verified hmmIBD using simulated data, benchmarked it against a previously published method for detecting IBD within populations, and demonstrated its utility using Plasmodium falciparum data from Cambodia and Ghana. Availability and Implemetation: Source code written in C99/C11-compliant C and requiring no external
doi:10.1101/188078
fatcat:eyyerpxayjbqjaxnfjfwedtz5e
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... s, is freely available for download at https://github.com/glipsnort/hmmIBD/releases, alongside test datasets.
MOESM3 of hmmIBD: software to infer pairwise identity by descent between haploid genotypes
2018
Figshare
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Additional file 3. Comparative study of isoRelate and hmmIBD and impact of assumed uniform recombination under hmmIBD. Full details and results of comparative study and exploration of the impact of assumed uniform recombination under hmmIBD.
doi:10.6084/m9.figshare.6274397
fatcat:jwnfezs7sbdf3c6lbpsh7zmbwm
Within-infection diversity of Plasmodium falciparum antigens reflects host-mediated selection
[article]
2017
bioRxiv
pre-print
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Aimee Taylor, Seth Redmond, and members of the Neafsey and Wirth research groups provided thoughtful comments and discussions. ...
doi:10.1101/212209
fatcat:znmn7de3x5etdjnsdkcf53mc2y
MOESM2 of Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing
2019
Figshare
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Additional file 2. Parallel amplicon sequencing error correction (PASEC) workflow.
doi:10.6084/m9.figshare.8426366.v1
fatcat:uvveyh3mhbbmdesawosftpkqhu
MOESM1 of Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing
2019
Figshare
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Additional file 1. Additional tables and figures.
doi:10.6084/m9.figshare.8426357
fatcat:bunpikiayff4vob565hvzay2z4
hmmIBD: software to infer pairwise identity by descent between haploid genotypes
2018
Malaria Journal
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We introduce hmmIBD, software to estimate pairwise identity by decent between haploid genomes, such as those of the malaria parasite, sampled from one or more populations. We verified hmmIBD using simulated data, benchmarked it against a previously published method for detecting IBD within populations, and demonstrated its utility using Plasmodium falciparum data from Cambodia and Ghana. Availability and Implemetation: Source code written in C99/C11-compliant C and requiring no external
doi:10.1186/s12936-018-2349-7
pmid:29764422
pmcid:PMC5952413
fatcat:zyoazbsgbrejtogpdcjd3lidlm
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... s, is freely available for download at https://github.com/glipsnort/hmmIBD/releases, alongside test datasets. Contact: sfs@broadinstitute.org Supplementary information: Supplementary data include Appendices S1, S2 and S3, and are available online. Wootton,J.C. et al. (2002) Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. 418, 18-21.
Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence
1986
Nucleic Acids Research
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At the 5' end of this transcript is the 35 nucleotide sequence that is present on the 5' end of Leishmania a-and B-tubulin mRNAs (Landfear, Miller, and Wirth; Molecular and Biochemical Parasitology in ...
Recently, we (Landfear, Miller, and Wirth; Molecular and Biochemical Parasitology in press) have demonstrated that the first 35 nucleotides at the 5' end of a-and $-tubulin mRNAs are identical to bases ...
doi:10.1093/nar/14.18.7341
pmid:2429261
pmcid:PMC311755
fatcat:k6inwgwerrfa3cxxk2ppgllh34
Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing
[article]
2018
bioRxiv
pre-print
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Acknowledgements We thank Anita Lerch for thoughtful comments on the manuscript, Katelyn Durfee for help preparing the mock samples, and members of the Neafsey and Wirth labs for helpful discussions throughout ...
doi:10.1101/453472
fatcat:xqgg7qlllbb2hcr46kutzjem5u
De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria
[article]
2017
bioRxiv
pre-print
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Funding for this work at the 568 Harvard TH Chan School of Public Health and the Broad Institute was provided by a 569 grant to DF Wirth from the Global Health Program at the Bill and Melinda Gates 570 ...
doi:10.1101/213397
fatcat:r7xg5zwwubezxi6nudm62hk2je
Development of a fluorescence polarization based assay for histone deacetylase ligand discovery
2008
Bioorganic & Medicinal Chemistry Letters
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Histone deacetylases (HDACs) regulate many important physiological processes and the discovery of small molecules that modulate HDAC activity has both academic and clinical relevance. HDAC inhibitors, most notably SAHA, have been pursued as cancer chemotherapeutics but may be useful in treating psychiatric disorders, malaria, and other diseases. Herein, we describe an inexpensive and robust assay, based on fluorescence polarization, for HDAC ligand discovery. The assay is well suited for
doi:10.1016/j.bmcl.2008.04.007
pmid:18430569
pmcid:PMC2408937
fatcat:q5mpq4x72rhg7ddtlatf4zltlm
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... roughput screening and enzyme kinetic studies. † contributed equally Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers
Inactivation of Plasmepsins 2 and 3 Sensitizes Plasmodium falciparum to the Antimalarial Drug Piperaquine
2018
Antimicrobial Agents and Chemotherapy
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Dihydroartemisinin-piperaquine (DHA-PPQ), the current frontline artemisinin combination therapy used to treat Plasmodium falciparum malaria in multiple Southeast Asian countries, is now increasingly failing in Cambodia, where artemisinin resistance is nearly fixed, which suggests that PPQ resistance has emerged and is spreading rapidly in the Greater Mekong Subregion. Recent reports have shown that amplification of the genes encoding plasmepsins 2 and 3 is a molecular marker of PPQ resistance;
doi:10.1128/aac.02309-17
pmid:29439977
fatcat:sfd7325cvjdq3mpvyx64bx6zmy
more »
... owever, whether these enzymes play a role in the mechanism of resistance is currently unknown. We show here that inactivating the genes encoding plasmepsin 2 or 3 individually in P. falciparum reference strain 3D7 results in hypersusceptibility to PPQ. Interestingly, no significant differences in the susceptibility to other antimalarials were observed, which suggests specific roles of plasmepsins 2 and 3 in PPQ susceptibility. The piperaquine hyper-sensitivity of the plasmepsin-2-and-3-inactivated lines provides direct evidence that these enzymes modulate parasite susceptibility to PPQ in the context of a single copy of PfMDR1 and independent of Kelch13 mutations conferring artemisinin resistance.
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