Filters








3,192 Hits in 4.6 sec

High-throughput SNP genotyping by single-tube PCR with Tm-shift primers

Jun Wang, Karen Chuang, Mandeep Ahluwalia, Sarika Patel, Nanette Umblas, Daniel Mirel, Russell Higuchi, Soren Germer
2005 BioTechniques  
Most recently, several highly multiplexed and ultra-high-throughput genotyping systems have become generally available (22) (23) (24) .  ...  Despite many recent advances in high-throughput single nucleotide polymorphism (SNP) genotyping technologies, there is still a great need for inexpensive and flexible methods with a reasonable throughput  ... 
doi:10.2144/000112028 pmid:16382908 fatcat:37wwefztcba27mmzutmztpfuzu

High-throughput profiling and analysis of plant responses over time to abiotic stress

Kira M. Veley, Jeffrey C. Berry, Sarah J. Fentress, Daniel P. Schachtman, Ivan Baxter, Rebecca Bart
2017 Plant Direct  
Additionally, ionomic profiling was conducted as an independent, low-cost, and high-throughput option for characterizing G x E, identifying the elements most affected by either genotype or treatment and  ...  We subjected a panel of 30 genetically diverse sorghum genotypes to a spectrum of nitrogen deprivation and measured responses using high-throughput phenotyping technology followed by ionomic profiling.  ...  Genotypes that displayed greater than average (blue) or less than average (magenta) growth are indicated. Error bars: 95% con dence intervals for both graphs.  ... 
doi:10.1002/pld3.23 pmid:31245669 pmcid:PMC6508565 fatcat:od2jczde2rdk7lidpcih6lgupa

High-throughput profiling and analysis of plant responses over time to abiotic stress [article]

Kira M. Veley, Jeffrey C. Berry, Sarah J. Fentress, Daniel P. Schachtman, Ivan Baxter, Rebecca Bart
2017 bioRxiv   pre-print
Additionally, ionomic profiling was conducted as an independent, low cost and high throughput option for characterizing G x E, identifying the elements most affected by either genotype or treatment and  ...  We subjected a panel of 30 genetically diverse sorghum genotypes to a spectrum of nitrogen deprivation and measured responses using high-throughput phenotyping technology followed by ionomic profiling.  ...  Genotypes that displayed greater than average (blue) or less than average (magenta) growth are indicated. Error bars: 95% con dence intervals for both graphs.  ... 
doi:10.1101/132787 fatcat:kc7ts23drng2fcljc734c7wpja

Optimization problems in dotted interval graphs

Danny Hermelin, Julián Mestre, Dror Rawitz
2014 Discrete Applied Mathematics  
The class of D-dotted interval (D-DI) graphs is the class of intersection graphs of arithmetic progressions with jump (common difference) at most D.  ...  Our algorithms are based on classical results in algorithmic graph theory and new structural properties of D-DI graphs that may be of independent interest.  ...  [2] in the context of high throughput genotyping. They used dotted intervals to model microsatellite polymorphisms which are used in a genotyping technique called microsatellite genotyping.  ... 
doi:10.1016/j.dam.2014.04.014 fatcat:xsvkydhd7zhtdopiw67bzidwjy

Deploying QTL-seq for rapid delineation of a potential candidate gene underlying major trait-associated QTL in chickpea

S. Das, H. D. Upadhyaya, D. Bajaj, A. Kujur, S. Badoni, Laxmi, V. Kumar, S. Tripathi, C. L. L. Gowda, S. Sharma, S. Singh, A. K. Tyagi (+1 others)
2015 DNA Research  
high seed weight mapping parents and homozygous individuals during seed development.  ...  The use of QTL-seq and classical QTL mapping in combination narrowed down the 1.37 Mb (comprising 177 genes) major SW QTL (CaqSW1.1) region into a 35 kb genomic interval on desi chickpea chromosome 1 containing  ...  Acknowledgements We are thankful to the Editor and referees for critically evaluating the manuscript and providing constructive comments.  ... 
doi:10.1093/dnares/dsv004 pmid:25922536 pmcid:PMC4463844 fatcat:jtuupwmobfa75ols26umta2ldi

Optimization Problems in Dotted Interval Graphs [chapter]

Danny Hermelin, Julián Mestre, Dror Rawitz
2012 Lecture Notes in Computer Science  
The class of D-dotted interval (D-DI) graphs is the class of intersection graphs of arithmetic progressions with jump (common difference) at most D.  ...  Our algorithms are based on classical results in algorithmic graph theory and new structural properties of D-DI graphs that may be of independent interest.  ...  [2] in the context of high throughput genotyping. They used dotted intervals to model microsatellite polymorphisms which are used in a genotyping technique called microsatellite genotyping.  ... 
doi:10.1007/978-3-642-34611-8_8 fatcat:cllmwbmtlzbr5lbcwjcre6m2vi

Epithelial-Myeloid cell crosstalk regulates acinar cell plasticity and pancreatic remodeling in mice

Yaqing Zhang, Wei Yan, Esha Mathew, Kevin T Kane, Arthur Brannon, Maeva Adoumie, Alekya Vinta, Howard C Crawford, Marina Pasca di Magliano
2017 eLife  
Thus, the cross-talk between epithelial cells and infiltrating myeloid cells determines the balance between tissue repair and carcinogenesis in the pancreas.  ...  Myeloid cells infiltrate the pancreas during the onset of pancreatic cancer, and promote carcinogenesis.  ...  is registered in the UK with company number FC030576 and branch number BR015634 at the address 1st Floor, 24 Hills Road, Cambridge CB2 1JP | August 2014 3 • High-throughput sequence data should be uploaded  ... 
doi:10.7554/elife.27388 pmid:28980940 pmcid:PMC5690281 fatcat:utc2csukcfg3rhphhlakgdro64

Physical mapping and InDel marker development for the restorer gene Rf2 in cytoplasmic male sterile CMS-D8 cotton

Juanjuan Feng, Xuexian Zhang, Meng Zhang, Liping Guo, Tingxiang Qi, Huini Tang, Haiyong Zhu, Hailin Wang, Xiuqin Qiao, Chaozhu Xing, Jianyong Wu
2021 BMC Genomics  
BSA resequencing technology, high-throughput SNP genotyping, and InDel markers were used to identify Rf2 locus on candidate interval of 1.48 Mb on chromosome D05.  ...  The selection of 24 single nucleotide polymorphisms (SNP) through high-throughput genotyping and the development insertion and deletion (InDel) markers were conducted to narrow down the candidate interval  ...  JJF, XXZ, and MZ performed the fertility survey, data analysis and qRT-PCR. JJF and JYW contributed to the preparation of the manuscript. All authors read and approved the final manuscript.  ... 
doi:10.1186/s12864-020-07342-y pmid:33407111 pmcid:PMC7789476 fatcat:622mcfdkejcm5aq6nc6yxyrww4

Comparative quantitative trait loci for silique length and seed weight in Brassica napus

Ying Fu, Dayong Wei, Hongli Dong, Yajun He, Yixin Cui, Jiaqin Mei, Huafang Wan, Jiana Li, Rod Snowdon, Wolfgang Friedt, Xiaorong Li, Wei Qian
2015 Scientific Reports  
With the rapid development of next-generation sequencing (NGS) technologies, some new strategies were proposed to take advantages of the power of high-throughput genotyping, e.g.  ...  By high density selective genotyping of DH lines with extreme phenotypes, using a Brassica single-nucleotide polymorphism (SNP) array, the QTL on chromosome A09 was narrowed, and aligned into 1.14-Mb region  ...  (2014BAD01B07), NSFC (31471529, 31401455, 31301351) and cstc (2014yykfA80008).  ... 
doi:10.1038/srep14407 pmid:26394547 pmcid:PMC4585775 fatcat:i3buopopyzffhldz3eq6e5lujq

High-throughput pedigree drawing

Ville-Petteri Mäkinen, Maija Parkkonen, Maija Wessman, Per-Henrik Groop, Timo Kanninen, Kimmo Kaski
2005 European Journal of Human Genetics  
Family trees have long been a valuable visual tool for geneticists in identifying clusters of inherited traits and genotypes.  ...  As more data are collected, drawing the graphs by hand becomes impractical and, for this reason, we have developed the pedigree software CraneFoot.  ...  The second category was covered by Tores and Barillot, 4 who presented an interval graph interpretation of perfectly drawable pedigrees.  ... 
doi:10.1038/sj.ejhg.5201430 pmid:15870825 fatcat:kz34qqgbrjbp5bc6d4rx6gkpta

Highly-multiplexed SNP genotyping for genetic mapping and germplasm diversity studies in pea

Chrystel Deulvot, Hélène Charrel, Amandine Marty, Françoise Jacquin, Cécile Donnadieu, Isabelle Lejeune-Hénaut, Judith Burstin, Grégoire Aubert
2010 BMC Genomics  
Conclusion: Our results show that the Illumina GoldenGate assay can be used successfully for high-throughput SNP genotyping of diverse germplasm in pea.  ...  This genotyping approach will simplify genotyping procedures for association mapping or diversity studies purposes and open new perspectives in legume genomics.  ...  Rond and H. Houtin in the development of the 'China' X 'Cameor' population. We warmly thank V. Fontaine and C.Rameau for sharing sequence data with us and D.  ... 
doi:10.1186/1471-2164-11-468 pmid:20701750 pmcid:PMC3091664 fatcat:e7v3bnm7h5evbekaz5fpisx6uq

QTL Mapping for Gummy Stem Blight Resistance in Watermelon (Citrullus spp.)

Eun Su Lee, Do-Sun Kim, Sang Gyu Kim, Yun-Chan Huh, Chang-Gi Back, Ye-Rin Lee, Muhammad Irfan Siddique, Koeun Han, Hye-Eun Lee, Jundae Lee
2021 Plants  
High-throughput markers were developed and validated for the selection of resistant QTLs using watermelon accessions, and commercial cultivars.  ...  LL and SB traits, respectively.  ...  Francisco, CA, USA) high-throughput (HT) genotyping platform.  ... 
doi:10.3390/plants10030500 pmid:33800297 fatcat:q6mh2hcftfczzfor3hy4qldx5i

Analysis of Population Genetic Diversity of CynoglossusSemilaevis in Three Groups from Northern China Sea

Bo Zhang, KeFeng Liu, DeBin Zheng, Lei Jia
2018 IOP Conference Series: Earth and Environment  
Two dimensional clustering results show that a small part of the region in Tianjin wild population had a small intervals with that of Tianjin cultured population, but the two principal components generally  ...  Principal component analysis was carried out by using software GCTA, and PCA showed that the contribution rates of the first two principal components PC1 and PC2 were 16.25% and 9.54%, respectively, and  ...  Through genome-wide high-throughput SNP screening and genotyping analysis, SNPs can be found for the genetic diversity analysis of population ( [6] ) The principal component analysis found that the genetic  ... 
doi:10.1088/1755-1315/178/1/012048 fatcat:j4xvvrwvgbhirclrxod66m6v4i

ScreenSeed as a novel high throughput seed germination phenotyping method

Nicolas Merieux, Pierre Cordier, Marie-Hélène Wagner, Sylvie Ducournau, Sophie Aligon, Dominique Job, Philippe Grappin, Edwin Grappin
2021 Scientific Reports  
AbstractA high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs.  ...  This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavior by incubating seeds in 96-well microplates under defined conditions and detecting  ...  made the partnership with GEVES (the French Variety and Seed Study and Control Group; Beaucouzé, France) possible.  ... 
doi:10.1038/s41598-020-79115-2 pmid:33446694 fatcat:vedqlnsrnfdujgyj2sdg653oe4

Dynamic plant height QTL revealed in maize through remote sensing phenotyping using a high-throughput unmanned aerial vehicle (UAV)

Xiaqing Wang, Ruyang Zhang, Wei Song, Liang Han, Xiaolei Liu, Xuan Sun, Meijie Luo, Kuan Chen, Yunxia Zhang, Hao Yang, Guijun Yang, Yanxin Zhao (+1 others)
2019 Scientific Reports  
We investigated the PH of diverse maize inbred lines (117 temperate lines, 135 tropical lines) at four growth stages using unmanned aerial vehicle high-throughput phenotypic platforms (UAV-HTPPs).  ...  Based on gene annotations and expression profiles, we identified candidate genes controlling PH. The PH data collected by the UAV-HTPPs were credible and the genetic mapping power was high.  ...  Figure 1 . 1 Field high-throughput phenotyping for plant height. (A) Digitally designed graphs depicting maize plants at four growth stages. Graphs were constructed using PlantCAD-maize software.  ... 
doi:10.1038/s41598-019-39448-z pmid:30837510 pmcid:PMC6401315 fatcat:4y5cpxhptjhnpmen5z6pbrkebq
« Previous Showing results 1 — 15 out of 3,192 results