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Compressed (Wu et al. 2018) ILSVRC2012-CLS 21.9 - CoViAR (Wu et al. 2018) - Compressed+Flow ILSVRC2012-CLS 24.1 - Asyn-TF (Sigurdsson et al. 2017) VGG16 RGB+Flow ImageNet 22.4 - MultiScale (TR) (Zhou ...arXiv:1911.09243v1 fatcat:satx6vo66fgvtneveg2trkc224
A new species, Bulbophyllum papuaense, was described and illustrated from Indonesia. Bulbophyllum papuaense is similar to Bulbophyllum tortuosum and B. muscohaerens but differs from them by having rhizome and pseudobulbs covered with papillose scales, caudate and ciliate petals, linear and ciliate lip.doi:10.3897/phytokeys.138.38714 pmid:31988607 pmcid:PMC6968976 fatcat:yp6rj5xmlzhqropd7snh442ng4
Genetic approaches such as retrovirus-mediated mutagenesis and cDNA expression libraries have contributed greatly to our understanding of signal transduction in mammalian cells. However, previously described methods for retroviral insertional mutagenesis are hindered by low mutagenesis rates and diculties in cloning mutated genes. cDNA expression library methods are usually celltype dependent and bias towards abundant and short messages. With the near completion of the genome projects,doi:10.1038/sj.onc.1203992 pmid:11146547 fatcat:kujhgvx4wfdqtmttnqscrlgixe
more »... ve genetic methods are needed where large numbers of genes can be more easily isolated and biochemically studied. We have developed a novel retrovirus-mediated genetic screening method in cultured cells. To achieve ecient and regulated mutagenesis, we constructed Enhanced Retroviral Mutagen (ERM) vectors that contained several engineered sequences (e.g., an ERM Tag and a splice donor) controlled by a tetracycline-responsive promoter. Endogenous genes can thus be randomly activated and tagged in a conditional system. NIH3T3 cells were used to screen for focusforming genes using the ERM strategy. We showed that these added sequences increased the screening eciency by 410-fold, and allowed more direct identi®cation of the genes targeted. Sequence analysis of *10% of the 4600 focus clones recovered revealed both known oncogenes and novel factors such as protein kinases and GTP/GDP exchange proteins. The ERM strategy should help to facilitate large-scale gene identi®cation in diverse pathways and integrate both genetic (with the completion of the genome projects) and functional information more readily. Oncogene (2000) 19, 5964 ± 5972. Enhanced retroviral mutagen-mediated genetic screens D Liu et al
The regulation of microRNA (miRNA) is closely related to methamphetamine (METH) addiction. Past studies have reported that miR-181a is associated with METH addiction, but the mechanism pathways remain elusive. On the basis of our past studies, which reported the endoplasmic reticulum-associated protein degradation (ERAD) mediated ubiquitin protein degradation of GABAAα1, which was involved in METH addiction. The present study, using qRT-PCR and bioinformatics analysis, further revealed thatdoi:10.3389/fnmol.2021.667725 pmid:34025353 pmcid:PMC8137846 fatcat:d2elprykwrgjjehlopthnfczsu
more »... 181a may be indirectly responsible for the METH addiction and downregulation of GABAAα1 through the regulation of ERAD.
In order to provide a reference for the correct forecasting of short-term heavy rainfall and better disaster prevention and mitigation services in Shanxi Province, China, it is very important to carry out systematic research on short-term heavy precipitation events in Shanxi Province. Based on hourly precipitation data during the flood season (May to September) from 109 meteorological stations in Shanxi, China in 1980-2015, the temporal and spatial variation characteristics of short-time heavydoi:10.4236/gep.2019.73011 fatcat:gvzlcxqkdzee7mkwgfwqzcs5zi
more »... ainfall during the flood season are analyzed by using wavelet analysis and Mann-Kendall test. The results show that the short-time heavy rainfall in the flood season in Shanxi Province is mainly at the grade of 20 -30 mm/h, with an average of 97 stations having short-time heavy rainfall each year, accounting for 89% of the total stations. The short-time heavy rainfall mainly concentrated in July and August, and the maximal rain intensity in history appeared at 23 -24 on June 17, 1991 in Yongji, Shanxi is 91.7 mm/h. During the flood season, the short-time heavy rainfalls always occur at 16 -18 pm, and have slightly different concentrated time in different months. The main peaks of June, July and August are at 16, 17 and 18 respectively, postponed for one hour. Short-time heavy rainfall overall has the distribution that the south is more than the north and the east less than the west in Shanxi area. In the last 36 years, short-time heavy rainfall has a slight increasing trend in Shanxi, but not significant. There is a clear 4-year period of oscillation and inter-decadal variation. It has a good correlation between the total precipitation and times of short-time heavy rainfall during the flood season. How to cite this paper: Tian, X.
In nature, fluid manipulations are ubiquitous in organisms, and they are crucial for many of their vital activities. Therefore, this process has also attracted widescale research attention. However, despite significant advances in fluid transportation research over the past few decades, it is still hugely challenging to achieve efficient and nondestructive droplet transportation owing to contamination effects and controllability problems in liquid transportation applications. To this end,doi:10.1002/advs.201900834 pmid:31508285 pmcid:PMC6724473 fatcat:6djljr4fgzbtvbxfpn4bvwsjzi
more »... ed by the motile microcilia of micro-organisms, the superhydrophobicity of lotus leaves, the underwater superoleophobicity of filefish skin, and pigeons' migration behavior, a novel manipulation strategy is developed for droplets motion. Specifically, herein, a superwettable magnetic microcilia array surface with a structure that is switchable by an external magnetic field is constructed for droplet manipulation. It is found that under external magnetic fields, the superhydrophobic magnetic microcilia array surface can continuously and directionally manipulate the water droplets in air and that the underwater superoleophobic magnetic microcilia array surface can control the oil droplets underwater. This work demonstrates that the nondestructive droplet transportation mechanism can be used for liquid transportation, droplet reactions, and micropipeline transmission, thus opening up an avenue for practical applications of droplet manipulation using intelligent microstructure surfaces.
The regions marked by the rectangle frames are the transposons (or remnant). 4508 ZHOU ET AL. ANTIMICROB. ...doi:10.1128/aac.01134-10 pmid:21788470 pmcid:PMC3187012 fatcat:nncoovunmbe33fjbp5yivf4ixe
Zhou, Y. Zhou, and J. ...doi:10.35833/mpce.2020.000501 fatcat:p3llwckhrfawzmltezrci2uyti
A study is conducted on a new airworthiness compliance verification method based on pilot-aircraft-environment complex system simulation. Verification scenarios are established by "block diagram" method based on airworthiness criteria. A pilot-aircraft-environment complex model is set up and a virtual flight testing method based on connection of MATLAB/Simulink and Flightgear is proposed. Special researches are conducted on the modeling of pilot manipulation stochastic parameters anddoi:10.1016/s1000-9361(11)60434-2 fatcat:omcmfp2rnbbpdldaixv4zuodoi
more »... n in critical situation. Unfavorable flight factors of certain scenario are analyzed, and reliability modeling of important system is researched. A distribution function of small probability event and the theory on risk probability measurement are studied. Nonlinear function is used to depict the relationship between the cumulative probability and the extremum of the critical parameter. A synthetic evaluation model is set up, modified genetic algorithm (MGA) is applied to ascertaining the distribution parameter in the model, and a more reasonable result is obtained. A clause about vehicle control functions (VCFs) verification in MIL-HDBK-516B is selected as an example to validate the practicability of the method.
On the contrary, the posture changes the surrounding ow eld and increases the exibility. 31 To further reveal the effect and exibility of studying the drag reduction of puffer, Zhou, Cui, et al. established ...doi:10.1039/d0ra08672j pmid:35424313 pmcid:PMC8694127 fatcat:66mswsg57ze6xonrlzz3vorgku
In traditional Chinese medical (TCM) science, tongue images can be observed for medical diagnosis; however, the tongue diagnosis of TCM is influenced by the subjective factors of doctors, and the diagnosis results vary from person to person. Quantitative TCM tongue diagnosis can improve the accuracy of diagnosis and increase the application value. In this paper, digital image processing and pattern recognition technologies are employed on mobile device to classify tongue images collected indoi:10.33851/jmis.2019.6.2.99 fatcat:s5qmac4qkbawrafbei44sgpxqm
more »... erent health states. First, through grayscale integral projection processing, the trough is found to localize the tongue body. Then the tongue body image is transferred from RGB color space to HSV color space, and the average H and S values are considered as the color features. Finally, the diagnosis results are obtained according to the relationship between the color characteristics and physical symptoms.
Molecular dynamics simulations are performed to study the growth mechanism of CH 4 -CO 2 mixed hydrate in x CO 2 = 75%, x CO 2 = 50%, and x CO 2 = 25% systems at T = 250 K, 255 K and 260 K, respectively. Our simulation results show that the growth rate of CH 4 -CO 2 mixed hydrate increases as the CO 2 concentration in the initial solution phase increases and the temperature decreases. Via hydrate formation, the composition of CO 2 in hydrate phase is higher than that in initial solution phasedoi:10.1016/s2095-4956(14)60208-4 fatcat:3dnnk4gksffxvilh63fwjffd3y
more »... d the encaging capacity of CO 2 in hydrates increases with the decrease in temperature. By analysis of the cage occupancy ratio of CH 4 molecules and CO 2 molecules in large cages to small cages, we find that CO 2 molecules are preferably encaged into the large cages of the hydrate crystal as compared with CH 4 molecules. Interestingly, CH 4 molecules and CO 2 molecules frequently replace with each other in some particular cage sites adjacent to hydrate/solution interface during the crystal growth process. These two species of guest molecules eventually act to stabilize the newly formed hydrates, with CO 2 molecules occupying large cages and CH 4 molecules occupying small cages in hydrate.
Hydrodynamic injection (HI) of hepatitis B virus (HBV) mouse model is an useful tool for HBV related research in vivo. However, only 40% of C57/BL6 mice injected with 10 μg HBV genome contained plasmid (pAAV-HBV1.2), serum HBsAg more than 6 months and none of the BALB/c mice injected with 10 μg pAAV-HBV1.2 plasmid DNA, serum HBsAg positive more than 4 weeks in the previous study. Methods: In this study, C57/BL6 and BALB/c mice were hydrodynamic injected with different doses of pAAV-HBV1.2doi:10.1186/s12985-017-0874-6 pmid:29070073 pmcid:PMC5657044 fatcat:35ec3zik5zbfnpxdvhzegmvld4
more »... d DNA. HBV related serum markers were detected by ELISA. ALT levels in the serum were measured using full automated biochemistry analyzer. HBcAg positive cells in the liver were detected by immunohistochemical staining. The mRNA levels of IRF3, ISGs including ISG15, OAS, PKR and immune factors including IFNγ, TNFα, TGFβ, IL-6, IL-10, PDL1 in liver of the mice were quantified by qRT-PCR. Results: The results showed that the mice injected with 100 μg high-concentration or 1 μg low-concentration of pAAV-HBV1.2 plasmid DNA did not excert dominant influence on HBV persistence. In contrast, injection of 5 μg intermediatedose of pAAV-HBV1.2 plasmid DNA led to significant prolonged HBsAg expression and HBV persistence in both C57/BL6 (80% of the mice with HBsAg positive more than 6 months) and BALB/c (60% of the mice with HBsAg positive more than 3 months) mice. IFNγ was significant up-regulated in liver of the mice injected with 1 μg or 100 μg pAAV-HBV1.2 plasmid DNA. TNFα was up-regulated significantly in liver of the mice injected with 100 μg pAAV-HBV1.2 plasmid DNA. Moreover, PDL1 was significant up-regulated in liver of the mice injected with 5 μg pAAV-HBV1.2 plasmid DNA. Conclusion: In this paper we demonstrated that, in the HBV HI mouse model, the concentration of injected pAAV-HBV1.2 plasmid DNA contributes to the diverse kinetics of HBsAg and HBeAg in the serum as well as HBcAg expression level in the liver, which then determined the HBV persisternce, while the antiviral factors IFNγ, TNFα as well as immune negative regulatory factor PDL1 play important roles on HBV persistence.
Bone fractures are common traumatic injuries of the musculoskeletal system. However, delayed union and non‑union fractures are a major clinical problem that present significant socioeconomic burden to patients and the public health sector. The bone‑resorbing osteoclasts and bone‑forming osteoblasts serve important roles in the fracture repair/healing process. Osteoclast deficiency or decreased osteoblast activity negatively impacts fracture healing. We previously demonstrated that the specificdoi:10.3892/mmr.2020.11209 pmid:32626968 pmcid:PMC7339621 fatcat:g3zoc6vlh5axbmeorhcpb4trfu
more »... eletion of the serine/threonine kinase 3‑phosphoinositide‑dependent protein kinase 1 (PDK1) in osteoclasts leads to abrogated osteoclast formation and bone resorption in response to receptor activator of nuclear factor‑κB in vitro and protected mice against ovariectomized‑induced bone loss and lipopolysaccharide‑induced osteolysis in vivo. Given the importance of osteoclasts in fracture repair, we hypothesized that the specific loss of PDK1 in osteoclasts will alter the fracture healing process. Mice of tibial fracture were constructed, and tibial specimens were sampled at 7‑, 14‑, 21‑ and 28‑days post‑fracture to observe the effect of PDK1 gene regulated osteoclasts on fracture healing process by X‑ray radiography, microcomputed tomography scanning, histomorphological staining and biomechanical testing. The present study revealed, using the tibial fracture model, that the specific deletion of the PDK1 gene in osteoclasts impeded the fracture healing process by delaying the resorption of the cartilaginous callus and subsequent remodeling of immature woven bone to structurally and mechanically ensure lamellar bone is stronger. No effect on osteoblast bone formation and osteogenesis was observed, thus indicating that delayed fracture healing is primarily due to defective osteoclast activity. These results provide important clinical implications for the use of anti‑resorptive agents, such as bisphosphonates, for the treatment of osteolytic conditions. Such anti‑resorptive therapies may detrimentally delay fracture healing and repair.
This paper investigates the effects of auditor-client disagreement disclosure on auditor resignations and audit fee charged by successor auditors. Using a matched sample of auditor changes over the period 2003-2016, we find that auditor resignations are more often accompanied by auditor-client disagreements. We also find that Big 4 auditors are more likely to resign from their engagements when they disagree with their clients. Further, we document that successor auditors charge higher auditdoi:10.19030/jabr.v36i1.10321 fatcat:3vf6nlaegnb7bmrtaa2wujvfem
more »... for firms that have disagreements with their predecessor auditors. Relative to non-Big 4 auditors, Big 4 successor auditors charge even higher audit fee for disagreement firms.
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