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Diode clamped gate driver-based high voltage pulse generator for electroporation

2018 Turkish Journal of Electrical Engineering and Computer Sciences  
The present study aims to explore a high voltage pulse generator for electroporation study.  ...  PEF food processing requires a high voltage pulse generator that produces high intensive pulses to be delivered to the food product.  ...  The high voltage pulse generator consists of two parts: a diode clamped gate driver circuit (DCGD) and the high voltage pulse generator.  ... 
doi:10.3906/elk-1710-133 fatcat:cw6ovx4tizazlgimb3lvi2g45y

Review on Driving Circuits for Wide-Bandgap Semiconductor Switching Devices for Mid- to High-Power Applications

Chao-Tsung Ma, Zhen-Huang Gu
2021 Micromachines  
Next, considerations regarding the design of driving circuits for WBG switching devices are addressed, and commercial drivers designed for WBG switching devices are explored.  ...  Wide-bandgap (WBG) material-based switching devices such as gallium nitride (GaN) high electron mobility transistors (HEMTs) and silicon carbide (SiC) metal-oxide-semiconductor field-effect transistors  ...  Acknowledgments: The author would like to thank the Ministry of Science and Technology (MOST) of Taiwan for financially support the energy-related research regarding key technologies in microgrids and  ... 
doi:10.3390/mi12010065 pmid:33430093 pmcid:PMC7826992 fatcat:nuc4g5c4effoxg7gs4ytf4b5uy

New High Voltage Pulse Generators

Abbas Pourzaki, Hossein Mirzaee
2009 Recent Patents on Electrical Engineering  
Methods of high voltage pulse generators based on various shape of pulse are also presented.  ...  In this paper, the design and structure of high-voltage pulse generator will be described.  ...  There are a lot of methods for implementation of high voltage pulse generators, some of them are introduced as below: High Voltage Pulse Generator Utilizing Marx Cells A high voltage pulse generator  ... 
doi:10.2174/1874476110902010065 fatcat:ajj5feqf5nbplfvnaztctgzbcq

Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon

Jessica E Messier, Hongmei Chen, Zhao-Lin Cai, Mingshan Xue
2018 eLife  
Light-gated chloride channels are emerging as promising optogenetic tools for inhibition of neural activity.  ...  for optogenetic inhibition.  ...  (H134R)-EYFP-Kv2.1C plasmid, Edward Boyden for the pAAV-hSyn-soCoChR-EGFP plasmid, Paul Pfaffinger for the Kv4.  ... 
doi:10.7554/elife.38506 pmid:30091701 fatcat:t2l53zs4wbfqlmchl3lt6ceqba

Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon [article]

Jessica Messier, Hongmei Chen, Zhao-Lin Cai, Mingshan Xue
2018 bioRxiv   pre-print
Light-gated chloride channels are emerging as promising optogenetic tools for inhibition of neural activity.  ...  for optogenetic inhibition.  ...  for the pAAV-CaMKIIa-iC++-TS-EYFP and pAAV-EF1a-DIO-iC++-TS-EYFP plasmids, Matthew Caudill and Massimo Scanziani for the pAAV-EF1a-DIO-iChloC-T2A-mCherry plasmid, Zhuo-Hua Pan for the pAAV-EF1a-DIO-hChR2  ... 
doi:10.1101/331165 fatcat:tnayylorr5huhlwbbimskjq5ma

Methodological Approach for Optogenetic Manipulation of Neonatal Neuronal Networks

Sebastian H. Bitzenhofer, Joachim Ahlbeck, Ileana L. Hanganu-Opatz
2017 Frontiers in Cellular Neuroscience  
We show that brain area-, layer-and cell type-specific expression of opsins by in utero electroporation (IUE), as exemplified for the medial prefrontal cortex (PFC) and hippocampus (HP), permits the manipulation  ...  Coordinated patterns of electrical activity are critical for the functional maturation of neuronal networks, yet their interrogation has proven difficult in the developing brain.  ...  ACKNOWLEDGMENTS We thank Annette Marquardt, Iris Ohmert and Achim Dahlmann for excellent technical assistance. ILH-O is member of the FENS Kavli Network of Excellence.  ... 
doi:10.3389/fncel.2017.00239 pmid:28848399 pmcid:PMC5554786 fatcat:j5catjrpv5hzzlh2skz43vakqe

Cardiac optogenetics

Emilia Entcheva
2013 American Journal of Physiology. Heart and Circulatory Physiology  
Optogenetics is an emerging technology for optical interrogation and control of biological function with high specificity and high spatiotemporal resolution.  ...  Mammalian cells and tissues can be sensitized to respond to light by a relatively simple and well-tolerated genetic modification using microbial opsins (light-gated ion channels and pumps).  ...  This is due to the stimulus (the actual induced photocurrent) being shaped based on instant real-time feedback about the membrane voltage; i.e., unlike electrical pulse stimulation, the ChR2 current pulse  ... 
doi:10.1152/ajpheart.00432.2012 pmid:23457014 pmcid:PMC3652095 fatcat:qtuj3fmb5vb67c65yv4yvamffa

Optical techniques in optogenetics

Samarendra K. Mohanty, Vasudevan Lakshminarayananan
2015 Journal of Modern Optics  
Further, emphasis is placed on advancement in optogenetics-associated light-based methods for controlling gene expression, spatially-controlled optogenetic stimulation and detection of cellular activities  ...  Optogenetics is an innovative technique for optical control of cells. This field has exploded over the past decade or so and has given rise to great advances in neuroscience.  ...  Bryan Black (UTA) for help in preparation of some portion of the text and figures.  ... 
doi:10.1080/09500340.2015.1010620 pmid:26412943 pmcid:PMC4582796 fatcat:mw2p6wykxbdmzbwplawv7yczba

Optogenetic identification of a rapid eye movement sleep modulatory circuit in the hypothalamus

Sonia Jego, Stephen D Glasgow, Carolina Gutierrez Herrera, Mats Ekstrand, Sean J Reed, Richard Boyce, Jeffrey Friedman, Denis Burdakov, Antoine R Adamantidis
2013 Nature Neuroscience  
Using optogenetic tools in newly-generated Tg(Pmch-Cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM sleep episode  ...  Carter and L. de Lecea for helpful comments on a previous version of the manuscript. We thank the Tidis Lab members for their technical help and comments.  ...  Optogenetic stimulation All optogenetic stimulations (5-ms pulses; 1-20 Hz) were generated by a waveform generator (Master 9) that triggers two blue-diode lasers (473 nm, LaserGlow).  ... 
doi:10.1038/nn.3522 pmid:24056699 pmcid:PMC4974078 fatcat:6bl5bka7cjdezh2b3npqrorti4

The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1

Xiaoyi Mo, Qiang Liu, Luna Gao, Chang Xie, Xin Wei, Peiyuan Pang, Quan Tian, Yue Gao, Youjing Zhang, Yuanyuan Wang, Tianchen Xiong, Bo Zhong (+2 others)
2022 BMC Biology  
Our results further demonstrate that this residue exerts a gating effect through hydrophobic interactions and support the existence of discrete domains for multimodal gating of TRPV1 channel.  ...  Conclusions Our results suggest TRPV1 is a co-receptor for 1,4-dioxane and that this accounts for its ability to dysregulate body nociceptive sensation.  ...  We also would like to thank members of Yao lab for comments and discussions, and the core facilities of College of Life Sciences at Wuhan University for technical help.  ... 
doi:10.1186/s12915-021-01211-0 pmid:34996439 pmcid:PMC8742357 fatcat:7dwwnu5qe5exzgf2y7bndpvdse

Targeting and Excitation of Photoactivatable Molecules: Design Considerations for Neurophysiology Experiments [chapter]

Eugene F. Civillico, J. Peter Rickgauer, Samuel S.-H. Wang
2011 Neuromethods  
We also compare current mature and emerging technologies for patterned light delivery, referring as much as possible to broadly applicable general principles.  ...  In this chapter, we take up general prefatory questions: how to determine which molecules are appropriate to use, and what type of compound delivery and light-targeting apparatus for photoactivation is  ...  One caution is that the voltages used to electroporate can damage developing tissue, leading to low viability depending on the position and geometry of the target tissue and electrodes.  ... 
doi:10.1007/978-1-61779-031-7_2 fatcat:mvrxnq53vrf4bhizbl5myzqu5i

Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics

Yoav Adam, Jeong J. Kim, Shan Lou, Yongxin Zhao, Michael E. Xie, Daan Brinks, Hao Wu, Mohammed A. Mostajo-Radji, Simon Kheifets, Vicente Parot, Selmaan Chettih, Katherine J. Williams (+12 others)
2019 Nature  
Here we developed improved near-infrared voltage indicators, high-speed microscopes and targeted gene expression schemes that enabled simultaneous in vivo recordings of supra- and subthreshold voltage  ...  Genetically encoded voltage indicators are a promising tool for these purposes; however, these have so far been limited to single-cell recordings with a marginal signal-to-noise ratio in vivo3-5.  ...  3 , but did not attain adequate signal-to-noise ratio (SNR) for voltage imaging in brain in vivo due to poor GEVI trafficking and high background fluorescence.  ... 
doi:10.1038/s41586-019-1166-7 pmid:31043747 pmcid:PMC6613938 fatcat:wyxzayosj5ef5euopip7ljt5jm

Optogenetic manipulation of neural and non-neural functions

Hiromu Yawo, Toshifumi Asano, Seiichiro Sakai, Toru Ishizuka
2013 Development, Growth and Differentiation  
In this review we discuss the potential of optogenetics with a focus on three major requirements for its application: (i) selection of the light-sensitive proteins optimal for optogenetic investigation  ...  , (ii) targeted expression of these selected proteins in a specific group of neurons, and (iii) targeted irradiation with high spatiotemporal resolution.  ...  When ChR2 is exogenously expressed in neurons, blue light irradiation evokes an inward current with membrane depolarization, which opens voltage-gated sodium channels and calcium channels, and generates  ... 
doi:10.1111/dgd.12053 pmid:23550617 fatcat:mhnx4iqcsbdhbgw74ds566k5dq

Optogenetics in Neural Systems

Ofer Yizhar, Lief E. Fenno, Thomas J. Davidson, Murtaza Mogri, Karl Deisseroth
2011 Neuron  
The effects provided by a channelrhodopsin when present in an axon terminal may act via the combined influence of voltage-gated Na + channels and voltage-gated Ca 2+ channels (perhaps along with, and under  ...  For instance, 473 nm and 532 nm DPSS lasers can reliably generate 1 ms pulses (though for pulses < 100 ms in duration, the average power during a pulse may be significantly less than the steady-state output  ... 
doi:10.1016/j.neuron.2011.06.004 pmid:21745635 fatcat:3mt35dewvvc2beyxmwvdei64cu

Optogenetic tools for modulating and probing the epileptic network

Mingrui Zhao, Rose Alleva, Hongtao Ma, Andy G.S. Daniel, Theodore H. Schwartz
2015 Epilepsy Research  
Hence, there is a continued need for novel therapies for those patients. A new technique called "optogenetics" may offer a new hope for these refractory patients.  ...  Optogenetics is a technology based on the combination of optics and genetics, which can control or record neural activity with light.  ...  Based on their actions, current antiepileptic drugs are conveniently categorized into those that (1) modulate voltage-gated ion channels (including sodium, calcium and potassium channels); (2) enhance  ... 
doi:10.1016/j.eplepsyres.2015.06.010 pmid:26354163 pmcid:PMC4567692 fatcat:4s6cgpw2wrcl5fuwwxro7speuu
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